scholarly journals Biocompatibility and Cellular Behavior of TiNbTa Alloy with Adapted Rigidity for the Replacement of Bone Tissue

Metals ◽  
2021 ◽  
Vol 11 (1) ◽  
pp. 130
Author(s):  
Mercè Giner ◽  
Ernesto Chicardi ◽  
Alzenira de Fátima Costa ◽  
Laura Santana ◽  
María Ángeles Vázquez-Gámez ◽  
...  
Keyword(s):  
Cortical Bone ◽  
Bone Tissue ◽  
Stress Shielding ◽  
Mechanical Resistance ◽  
Cellular Behavior ◽  
Biomechanical Behavior ◽  
Functional Behavior ◽  
Bone Replacement ◽  
Osteoblast Adhesion

In this work, the mechanical and bio-functional behavior of a TiNbTa alloy is evaluated as a potential prosthetic biomaterial used for cortical bone replacement. The results are compared with the reference Ti c.p. used as biomaterials for bone-replacement implants. The estimated mechanical behavior for TiNbTa foams was also compared with the experimental Ti c.p. foams fabricated by the authors in previous studies. A TiNbTa alloy with a 20–30% porosity could be a candidate for the replacement of cortical bone, while levels of 80% would allow the manufacture of implants for the replacement of trabecular bone tissue. Regarding biocompatibility, in vitro TiNbTa, cellular responses (osteoblast adhesion and proliferation) were compared with cell growth in Ti c.p. samples. Cell adhesion (presence of filopodia) and propagation were promoted. The TiNbTa samples had a bioactive response similar to that of Ti c.p. However, TiNbTa samples show a better balance of bio-functional behavior (promoting osseointegration) and biomechanical behavior (solving the stress-shielding phenomenon and guaranteeing mechanical resistance).

In Vitro Characterization of 3D Beta-Tricalcium Phosphate Scaffolds Reinforced with Phosphate Based-Bioactive Glass for Bone Replacement

2016 ◽  
Vol 720 ◽  
pp. 108-113
Author(s):  
Criseida Ruiz-Aguilar ◽  
E.A. Aguilar-Reyes ◽  
Ana Edith Higareda-Mendoza ◽  
C.A. León-Patiño
Keyword(s):  
Heat Treatment ◽  
Tissue Engineering ◽  
Cell Proliferation ◽  
Bone Tissue ◽  
Present Contribution ◽  
Beta Tricalcium Phosphate ◽  
Bone Replacement ◽  
Factor Therapy

Bone tissue engineering is an excellent alternative to reduce bone disorders and conditions, by inducing new functional bone regeneration starting from the synthesis of the biomaterials and then the combination of cell and factor therapy. In the present contribution, the scaffolds were made with a ratio of 80 wt.% of β-TCP and 20 wt. % of phosphate-based bioglass, in addition the phosphate-based bioglass was reinforced with zirconia in different amounts (0, 0.5 and 1.0 mol%) with the aim to reduce the dissolution rate, improve the osteoconduction and the osteogenesis of the bone tissue. The results obtained by μCT of the scaffolds containing zirconia showed a wide pore size distribution between 1.5 and 303 μm. AlamarBlue assays showed that the cell proliferation of MC3T3-E1 preosteoblasts scaffolds were sixfold increase in relation to the number of the initial cells. FE-SEM helped to observe the cauliflower structure of HA and DRX showed that crystalline phases formed after heat treatment were (NaCaPO4 and NaZr5PO4) owing both to the crystallization and combination of the bioglass and β-TCP .

Biomechanical evaluation of different implant–abutment connections, retention systems, and restorative materials in the implant-supported single crowns using 3D finite element

2021 ◽  
Author(s):  
Cleidiel Aparecido Araujo Lemos ◽  
Fellippo Ramos Verri ◽  
Pedro Yoshito Noritomi ◽  
Victor Eduardo Souza Batista ◽  
Ronaldo Silva Cruz ◽  
...  
Keyword(s):  
Finite Element ◽  
Cortical Bone ◽  
Bone Tissue ◽  
Von Mises Stress ◽  
Element Analysis ◽  
Biomechanical Behavior ◽  
Retention System ◽  
Implant Abutment ◽  
Fixation Screw ◽  
Metal Ceramic

This is an in silico study aimed to evaluate the biomechanical influence of different implant–abutment interfaces (external hexagon and Morse taper implants), retention systems (cement- and screw-retained), and restorative crowns (metal–ceramic and monolithic) using three-dimensional finite element analysis (3D-FEA). Eight 3D models were simulated for the maxillary first molar area using InVesalius, Rhinoceros, and SolidWorks and processed using the Femap and NEi Nastran softwares. Axial and oblique forces of 200 N and 100 N, respectively, were applied on the occlusal surface of the prostheses. Microstrain and von Mises stress maps were used to evaluate the deformation (cortical bone tissue) and stress (implants/fixation screws/crowns), respectively for each model. For both loadings, Morse taper implants had lower microstrain values than the external hexagon implants. The retention system did not affect microstrain on the cortical bone tissue under both loadings. However, the cemented prosthesis displayed higher stress with the fixation screw than the external hexagon implants. No difference was observed between the metal–ceramic and zirconia monolithic crowns in terms of microstrain and stress distribution on the cortical bone, implants or components. Morse taper implants can be considered as a good alternative for dental implant rehabilitation because they demonstrated better biomechanical behavior for the bone and fixation screw as compared to external hexagon implants. Cement-retained prosthesis increased the stress on the fixation screw of the external hexagon implants, thereby increasing the risk of screw loosening/fracture in the posterior maxillary area. The use of metal–ceramic or monolithic crowns did not affect the biomechanical behavior of the evaluated structures.

scholarly journals Biomechanical Loading Comparison between Titanium and Unsintered Hydroxyapatite/Poly-L-Lactide Plate System for Fixation of Mandibular Subcondylar Fractures

Materials ◽  
2019 ◽  
Vol 12 (9) ◽  
pp. 1557 ◽  
Author(s):  
Shintaro Sukegawa ◽  
Takahiro Kanno ◽  
Norio Yamamoto ◽  
Keisuke Nakano ◽  
Kiyofumi Takabatake ◽  
...  
Keyword(s):  
Shear Strength ◽  
Plate Fixation ◽  
In Vitro Study ◽  
Fixation Method ◽  
Mechanical Resistance ◽  
Titanium Plate ◽  
Biomechanical Behavior ◽  
Bioresorbable Plate ◽  
Plate System

Osteosynthesis absorbable materials made of uncalcined and unsintered hydroxyapatite (u-HA) particles, poly-l-lactide (PLLA), and u-HA/PLLA are bioresorbable, and these plate systems have feasible bioactive osteoconductive capacities. However, their strength and stability for fixation in mandibular subcondylar fractures remain unclear. This in vitro study aimed to assess the biomechanical strength of u-HA/PLLA bioresorbable plate systems after internal fixation of mandibular subcondylar fractures. Tensile and shear strength were measured for each u-HA/PLLA and titanium plate system. To evaluate biomechanical behavior, 20 hemimandible replicas were divided into 10 groups, each comprising a titanium plate and a bioresorbable plate. A linear load was applied anteroposteriorly and lateromedially to each group to simulate the muscular forces in mandibular condylar fractures. All samples were analyzed for each displacement load and the displacement obtained by the maximum load. Tensile and shear strength of the u-HA/PLLA plate were each approximately 45% of those of the titanium plates. Mechanical resistance was worst in the u-HA/PLLA plate initially loaded anteroposteriorly. Titanium plates showed the best mechanical resistance during lateromedial loading. Notably, both plates showed similar resistance when a lateromedially load was applied. In the biomechanical evaluation of mandibular condylar fracture treatment, the u-HA/PLLA plates had sufficiently high resistance in the two-plate fixation method.

scholarly journals Biomechanical Loading Comparison between Titanium and Bioactive Resorbable Screw Systems for Fixation of Intracapsular Condylar Head Fractures

Materials ◽  
2020 ◽  
Vol 13 (14) ◽  
pp. 3153
Author(s):  
Shintaro Sukegawa ◽  
Norio Yamamoto ◽  
Keisuke Nakano ◽  
Kiyofumi Takabatake ◽  
Hotaka Kawai ◽  
...  
Keyword(s):  
In Vitro Study ◽  
Small Displacement ◽  
Mechanical Resistance ◽  
Biomechanical Behavior ◽  
Model Study ◽  
Linear Load ◽  
Titanium Screws ◽  
Significant Difference ◽  
Condylar Head

Osteosynthesis resorbable materials made of uncalcined and unsintered hydroxyapatite (u-HA) particles, poly-L-lactide (PLLA), are bioresorbable, and these materials have feasible bioactive/osteoconductive capacities. However, their strength and stability for fixation in mandibular condylar head fractures remain unclear. This in vitro study aimed to assess the biomechanical strength of u-HA/PLLA screws after the internal fixation of condylar head fractures. To evaluate their biomechanical behavior, 32 hemimandible replicas were divided into eight groups, each consisting of single-screw and double-screw fixations with titanium or u-HA/PLLA screws. A linear load was applied as vertical and horizontal load to each group to simulate the muscular forces in condylar head fractures. Samples were examined for 0.5, 1, 2, and 3-mm displacement loads. Two screws were needed for stable fixation of the mandibular condylar head fracture during biomechanical evaluation. After screw fixation for condylar head fractures, the titanium screws model was slightly more resistant to vertical and horizontal movement with a load for a small displacement than the u-HA/PLLA screws model. There was no statistically significant difference with load for large displacements. The u-HA/PLLA screw has a low mechanical resistance under small displacement loading compared with titanium within the limits of the mandibular head fracture model study.

scholarly journals Bone-like ceramic scaffolds designed with bioinspired porosity induce a different stem cell response

2021 ◽  
Vol 32 (1) ◽  
Author(s):  
Silvia Panseri ◽  
Monica Montesi ◽  
Dominique Hautcoeur ◽  
Samuele M. Dozio ◽  
Shaan Chamary ◽  
...  
Keyword(s):  
Cortical Bone ◽  
Bone Tissue ◽  
Interstitial Fluid Flow ◽  
Bone Maturation ◽  
Native Bone ◽  
Multi Scale ◽  
Bone Scaffolds ◽  
Biomimetic Scaffolds ◽  
Biomaterial Science

AbstractBiomaterial science increasingly seeks more biomimetic scaffolds that functionally augment the native bone tissue. In this paper, a new concept of a structural scaffold design is presented where the physiological multi-scale architecture is fully incorporated in a single-scaffold solution. Hydroxyapatite (HA) and β-tricalcium phosphate (β-TCP) bioceramic scaffolds with different bioinspired porosity, mimicking the spongy and cortical bone tissue, were studied. In vitro experiments, looking at the mesenchymal stem cells behaviour, were conducted in a perfusion bioreactor that mimics the physiological conditions in terms of interstitial fluid flow and associated induced shear stress. All the biomaterials enhanced cell adhesion and cell viability. Cortical bone scaffolds, with an aligned architecture, induced an overexpression of several late stage genes involved in the process of osteogenic differentiation compared to the spongy bone scaffolds. This study reveals the exciting prospect of bioinspired porous designed ceramic scaffolds that combines both cortical and cancellous bone in a single ceramic bone graft. It is prospected that dual core shell scaffold could significantly modulate osteogenic processes, once implanted in patients, rapidly forming mature bone tissue at the tissue interface, followed by subsequent bone maturation in the inner spongy structure.

scholarly journals In-Vitro Assessment of Synthesized Hydroxyapatite Specimen in Simulated Body Fluid (SBF)

2020 ◽  
Vol 9 (4) ◽  
pp. 2782-2786
Keyword(s):  
Tissue Engineering ◽  
Simulated Body Fluid ◽  
Bone Tissue ◽  
Body Fluid ◽  
Bone Cancer ◽  
The Body ◽  
Precipitation Method ◽  
Bone Replacement ◽  
In Vitro Assessment

Recently, the patient that requires bone replacement has increase, especially the patients who suffering from bone cancer, trauma and ageing. This attracts attention of researchers related to biomaterial fields to synthesis materials from biomaterials waste for bone tissue replacements. Hydroxyapatite was identified as a suitable source for bone substitution due its excellent bioactivity and biocompatibility. The strategies for tissue engineering include developing those cells to form the required tissue/organ in-vitro before inserting them into the body. This study is aimed to investigate In-Vitro properties of hydroxyapatite (HAp) specimens synthesize from the clamshells via precipitation method. HAp moulded specimen were immersed in the simulated body fluid (SBF). It displayed that the development of apatite layers materialized in the surface of HAp after being immersed for 25 days in the 1.5SBF.

Directed Osteoblast Adhesion at Particle Boundaries: Promises for Nanophase Metals

2004 ◽  
Vol 823 ◽  
Author(s):  
Venu Perla ◽  
Jeremiah U. Ejiofor ◽  
Thomas J. Webster
Keyword(s):  
Unit Area ◽  
Stress Shielding ◽  
Bone Cell ◽  
Steady Growth ◽  
Osteoblast Adhesion ◽  
Surface Particle ◽  
Metallic Corrosion ◽  
Orthopedic Applications ◽  
Particle Boundary

AbstractIn spite of under performance, metals and metal alloys are currently being used in orthopedic implantable devices. Poor osseointegration, severe stress shielding, bone cell death and eventual necrotic bone resulting from the generation of wear debris are known to be some of the reasons responsible for their under performance. In addition, metallic corrosion products may also initiate cancer. Steady growth in the use of metals in orthopedic applications inspired researchers to deal with these problems in an integrated way. When conventional Ti, Ti6Al4V, and CoCrMo surfaces were modified to the nano-range, this study showed increased percentages of osteoblast (bone forming cell) adhesion on nanophase metals. Moreover, larger amounts of osteoblast adhesion was related to quantitative increases in the total length of particle boundaries per unit area and the total number of pores between surface particles per unit area, and the surface particle boundary index (SPBI) of nanophase metals. Additionally, we have developed a novel anticarcinogenic orthopedic metalloid, selenium (Se). When micron range surface particles of Se compacts were modified to the nano-range by chemical etching, we found positive relationships between directed osteoblast adhesion and various particle boundary parameters mentioned above under in vitro conditions. These results provided the first evidence to utilize nanosurface Se as an anticarcinogenic and bio-inspiring material for future applications in orthopedic metallic devices.

scholarly journals Strength and Biocompatibility of Heparin-Based Calcium Phosphate Cement Grafted with Ferulic Acid

Polymers ◽  
2021 ◽  
Vol 13 (13) ◽  
pp. 2219
Author(s):  
Kai-Chi Chang ◽  
Jian-Chih Chen ◽  
I-Tse Cheng ◽  
Ssu-Meng Haung ◽  
Shih-Ming Liu ◽  
...  
Keyword(s):  
Calcium Phosphate ◽  
Ferulic Acid ◽  
Bone Tissue ◽  
Calcium Phosphate Cement ◽  
Bone Mineralization ◽  
Stress Shielding ◽  
Biomimetic Synthesis ◽  
Bone Tissue Regeneration ◽  
Carbonated Apatites

The biomimetic synthesis of carbonated apatites by biomolecule-based templates is a promising way for broadening apatite applications in bone tissue regeneration. In this work, heparin was used as an organic template to prepare uniform carbonate-based apatite nanorods (CHA) and graft ferulic acid (F-CHA) for enhanced bone mineralization. Next, by combining calcium phosphate cement (CPC) with different F-CHA/CPC ratios, a new type of injectable bone cement combined with F-CHA bioactive apatite was developed (CPC + F-CHA). The physicochemical properties, biocompatibility, and mineralization potential of the CPC + F-CHA composites were determined in vitro. The experimental results confirmed the preparation of highly biocompatible CHA and the compatibility of F-CHA with CPC. Although CPC + F-CHA composites with F-CHA (2.5 wt%, 5 wt%, and 10 wt%) showed a significant reduction in compressive strength (CS), compositing CPC with 10 wt% F-CHA yielded a CS suitable for orthopedic repair (CS still larger than 30 MPa). Spectroscopic and phase analyses revealed that the phase of the hydrothermally synthesized CHA product was not modified by the heparin template. Injection and disintegration tests indicated that the CPC + F-CHA composites have good biocompatibility even at 10 wt% F-CHA. D1 osteoprogenitor cells were cultured with the composites for 7 days in vitro, and the CPC + 10%F-CHA group demonstrated significantly promoted cell mineralization compared with other groups. Given these results, the use of over 10% F-CHA in CPC composites should be avoided if the latter is to be applied to load-bearing areas. A stress-shielding device may also be recommended to stabilize these areas. These newly developed biocompatible CPC + F-CHA have great potential as osteoconductive bone fillers for bone tissue engineering.

Pullout Strength of Screws in Foal Third Metacarpal Bone after Overdrilling a 4.5 mm Hole

1998 ◽  
Vol 11 (04) ◽  
pp. 200-204 ◽  
Author(s):  
K. Kelly ◽  
G. S. Martin ◽  
D. J. Burba ◽  
S. A. Sedrish ◽  
R. M. Moore
Keyword(s):  
Cortical Bone ◽  
Metacarpal Bone ◽  
Pullout Strength ◽  
Cancellous Screw ◽  
Bone Screw ◽  
Screw Insertion ◽  
Holding Power ◽  
Cortical Screw ◽  
Metaphyseal Bone

SummaryThe purpose of the study was to determine and to compare the in vitro pullout strength of 5.5 mm cortical versus 6.5 mm cancellous bone screws inserted in the diaphysis and metaphysis of foal third metacarpal (MCIII) bones in threaded 4.5 mm cortical bone screw insertion holes that were then overdrilled with a 4.5 mm drill bit. This information is relevant to the selection of a replacement screw if a 4.5 mm cortical screw is stripped during orthopaedic surgery. In vitro pullout tests were performed in two independent cadaver studies, each consisting of 12 foal MCIII bones. Two 4.5 mm cortical screws were placed either in the mid-diaphysis (study 1) or distal metaphysis (study 2) of MCIII bones. The holes were then overdrilled with a 4.5 mm bit and had either a 5.5 mm cortical or a 6.5 mm cancellous screw inserted; screw pullout tests were performed at a rate of 0.04 mm/s until screw or bone failure occurred.The bone failed in all of the tests in the diaphyseal and metaphyseal bone. The holding power for 6.5 mm cancellous screws was significantly (p <0.05) greater than for 5.5 mm cortical screws in both the diaphysis and metaphysis. There was not any difference in the holding power of screws in either the diaphysis or the metaphysis between proximal and distal screw holes.If a 4.5 mm cortical bone screw strips in MCIII diaphyseal or metaphyseal bone of foals, a 6.5 mm cancellous screw would provide greater holding power than a 5.5 mm cortical screw.In order to provide information regarding selection of a replacement screw if a 4.5 mm cortical screw is stripped, the in vitro pullout strength was determined for 5.5 mm cortical and 6.5 mm cancellous screws inserted in third metacarpal diaphyseal and metaphyseal bone of foals in which threaded 4.5 mm cortical bone screw insertion holes had been overdrilled with a 4.5 mm bit. The holding power of the 6.5 mm cancellous screw was significantly greater than the 5.5 mm cortical screw in both the diaphysis and metaphysis of foal third metacarpal bone. Thus, it appears that if a 4.5 mm cortical screw is stripped during orthopaedic surgery in foals, a 6.5 mm cancellous screw would provide superior holding power.

Sign in / Sign up

Export Citation Format

Share Document