scholarly journals Creation of Artificial Cell-Like Structures Promoted by Microfluidics Technologies

Micromachines ◽  
2019 ◽  
Vol 10 (4) ◽  
pp. 216 ◽  
Author(s):  
Yusuke Sato ◽  
Masahiro Takinoue
Keyword(s):  
Low Cost ◽  
Sample Volume ◽  
Future Perspective ◽  
Biological Research ◽  
Artificial Cells ◽  
Research Fields ◽  
Artificial Cell ◽  
Biological Phenomena ◽  
The Creation ◽  
Oil Emulsions

The creation of artificial cells is an immensely challenging task in science. Artificial cells contribute to revealing the mechanisms of biological systems and deepening our understanding of them. The progress of versatile biological research fields has clarified many biological phenomena, and various artificial cell models have been proposed in these fields. Microfluidics provides useful technologies for the study of artificial cells because it allows the fabrication of cell-like compartments, including water-in-oil emulsions and giant unilamellar vesicles. Furthermore, microfluidics also allows the mimicry of cellular functions with chip devices based on sophisticated chamber design. In this review, we describe contributions of microfluidics to the study of artificial cells. Although typical microfluidic methods are useful for the creation of artificial-cell compartments, recent methods provide further benefits, including low-cost fabrication and a reduction of the sample volume. Microfluidics also allows us to create multi-compartments, compartments with artificial organelles, and on-chip artificial cells. We discuss these topics and the future perspective of microfluidics for the study of artificial cells and molecular robotics.

scholarly journals Artificial cell mimics as simplified models for the study of cell biology

2017 ◽  
Vol 242 (13) ◽  
pp. 1309-1317 ◽  
Author(s):  
Ali Salehi-Reyhani ◽  
Oscar Ces ◽  
Yuval Elani
Keyword(s):  
Cell Biology ◽  
Chemical Species ◽  
Experimental Models ◽  
Artificial Cells ◽  
Simplified Models ◽  
Future Directions ◽  
Artificial Cell ◽  
Biological Phenomena ◽  
Emerging Trends ◽  
Wide Range

Living cells are hugely complex chemical systems composed of a milieu of distinct chemical species (including DNA, proteins, lipids, and metabolites) interconnected with one another through a vast web of interactions: this complexity renders the study of cell biology in a quantitative and systematic manner a difficult task. There has been an increasing drive towards the utilization of artificial cells as cell mimics to alleviate this, a development that has been aided by recent advances in artificial cell construction. Cell mimics are simplified cell-like structures, composed from the bottom-up with precisely defined and tunable compositions. They allow specific facets of cell biology to be studied in isolation, in a simplified environment where control of variables can be achieved without interference from a living and responsive cell. This mini-review outlines the core principles of this approach and surveys recent key investigations that use cell mimics to address a wide range of biological questions. It will also place the field in the context of emerging trends, discuss the associated limitations, and outline future directions of the field. Impact statement Recent years have seen an increasing drive to construct cell mimics and use them as simplified experimental models to replicate and understand biological phenomena in a well-defined and controlled system. By summarizing the advances in this burgeoning field, and using case studies as a basis for discussion on the limitations and future directions of this approach, it is hoped that this minireview will spur others in the experimental biology community to use artificial cells as simplified models with which to probe biological systems.

scholarly journals Demonstration of a Label-Free and Low-Cost Optical Cavity-Based Biosensor Using Streptavidin and C-Reactive Protein

Biosensors ◽  
2020 ◽  
Vol 11 (1) ◽  
pp. 4
Author(s):  
Donggee Rho ◽  
Seunghyun Kim
Keyword(s):  
Limit Of Detection ◽  
Point Of Care ◽  
Low Cost ◽  
Optical Cavity ◽  
Sample Volume ◽  
Small Sample ◽  
Label Free ◽  
C Reactive Protein ◽  
Reactive Protein ◽  
Cavity Structure

An optical cavity-based biosensor (OCB) has been developed for point-of-care (POC) applications. This label-free biosensor employs low-cost components and simple fabrication processes to lower the overall cost while achieving high sensitivity using a differential detection method. To experimentally demonstrate its limit of detection (LOD), we conducted biosensing experiments with streptavidin and C-reactive protein (CRP). The optical cavity structure was optimized further for better sensitivity and easier fluid control. We utilized the polymer swelling property to fine-tune the optical cavity width, which significantly improved the success rate to produce measurable samples. Four different concentrations of streptavidin were tested in triplicate, and the LOD of the OCB was determined to be 1.35 nM. The OCB also successfully detected three different concentrations of human CRP using biotinylated CRP antibody. The LOD for CRP detection was 377 pM. All measurements were done using a small sample volume of 15 µL within 30 min. By reducing the sensing area, improving the functionalization and passivation processes, and increasing the sample volume, the LOD of the OCB are estimated to be reduced further to the femto-molar range. Overall, the demonstrated capability of the OCB in the present work shows great potential to be used as a promising POC biosensor.

scholarly journals Toward Experimental Evolution with Giant Vesicles

Life ◽  
2018 ◽  
Vol 8 (4) ◽  
pp. 53 ◽  
Author(s):  
Hironori Sugiyama ◽  
Taro Toyota
Keyword(s):  
Chemical Evolution ◽  
Self Assembly ◽  
Experimental Evolution ◽  
Microfluidic Devices ◽  
Future Perspective ◽  
Cell Models ◽  
Giant Vesicles ◽  
Chemical Models ◽  
Recent Developments ◽  
Oil Emulsions

Experimental evolution in chemical models of cells could reveal the fundamental mechanisms of cells today. Various chemical cell models, water-in-oil emulsions, oil-on-water droplets, and vesicles have been constructed in order to conduct research on experimental evolution. In this review, firstly, recent studies with these candidate models are introduced and discussed with regards to the two hierarchical directions of experimental evolution (chemical evolution and evolution of a molecular self-assembly). Secondly, we suggest giant vesicles (GVs), which have diameters larger than 1 µm, as promising chemical cell models for studying experimental evolution. Thirdly, since technical difficulties still exist in conventional GV experiments, recent developments of microfluidic devices to deal with GVs are reviewed with regards to the realization of open-ended evolution in GVs. Finally, as a future perspective, we link the concept of messy chemistry to the promising, unexplored direction of experimental evolution in GVs.

scholarly journals A Survey on Leukemia Detection using Image Processing Techniques

2021 ◽  
Vol 309 ◽  
pp. 01111
Author(s):  
Mohammed Junaid Ahmed ◽  
Padmalaya Nayak
Keyword(s):  
Deep Learning ◽  
Low Cost ◽  
Research Papers ◽  
Survey Paper ◽  
Machine Learning Methods ◽  
Research Fields ◽  
Dataset Size ◽  
Detection And Diagnosis ◽  
Processing Techniques ◽  
Late Works

Leukemia detection and diagnosis by inspecting the blood cell images is an intriguing and dynamic exploration region in both the Artificial Intelligence and Medical research fields. There are numerous procedures created to look at blood tests to identify leukemia illness, these strategies are the customary methods and the deep learning (DL) strategy. This survey paper presents a review on the distinctive conventional strategies and Deep Learning and Machine Learning methods towards that have been utilized in leukemia illness diagnosis dependent on platelets images and to analyze between the two methodologies in nature of appraisal, exactness, cost and speed. This article covers 11 research papers, 9 of these examinations were in customary strategies which utilized image handling and AI (ML) calculations, such as, K-closest neighbor (KNN), K-means, SVM, Naïve Bayes, and 2 investigations in cutting edge procedures which utilized Deep Learning, especially Convolutional Neural Networks (CNNs) which is the most generally utilized in the field leukemia detection since it is profoundly precise, quick, and has the smallest expense. What's more, it dissects various late works that have been presented in the field including the dataset size, the pre-owned procedures, the acquired outcomes, and so forth. At last, in view of the led study, it very well may be reasoned that the proposed framework CNN was accomplishing immense triumphs in the field whether in regards to highlights extraction or classification time, precision and also a best low cost in the identification of leukemia.

scholarly journals Exploiting Textured 3D Models for Developing Serious Games

2015 ◽  
Vol XL-5/W7 ◽  
pp. 249-255 ◽  
Author(s):  
G. Kontogianni ◽  
A. Georgopoulos
Keyword(s):  
Serious Games ◽  
Laser Scanner ◽  
3D Models ◽  
Serious Game ◽  
Educational Game ◽  
Future Perspective ◽  
Production Methods ◽  
Military Applications ◽  
The Creation ◽  
Textured Models

Digital technologies have affected significantly many fields of computer graphics such as Games and especially the field of the Serious Games. These games are usually used for educational proposes in many fields such as Health Care, Military applications, Education, Government etc. Especially Digital Cultural Heritage is a scientific area that Serious Games are applied and lately many applications appear in the related literature. Realistic 3D textured models which have been produced using different photogrammetric methods could be a useful tool for the creation of Serious Game applications in order to make the final result more realistic and close to the reality. The basic goal of this paper is how 3D textured models which are produced by photogrammetric methods can be useful for developing a more realistic environment of a Serious Game. The application of this project aims at the creation of an educational game for the Ancient Agora of Athens. The 3D models used vary not only as far as their production methods (i.e. Time of Flight laser scanner, Structure from Motion, Virtual historical reconstruction etc.) is concerned, but also as far as their era as some of them illustrated according to their existing situation and some others according to how these monuments looked like in the past. The Unity 3D<sup>®</sup> game developing environment was used for creating this application, in which all these models were inserted in the same file format. For the application two diachronic virtual tours of the Athenian Agora were produced. The first one illustrates the Agora as it is today and the second one at the 2nd century A.D. Finally the future perspective for the evolution of this game is presented which includes the addition of some questions that the user will be able to answer. Finally an evaluation is scheduled to be performed at the end of the project.

scholarly journals Bouwen aan jezelf door te bouwen voor een ander. Een architecturale analyse van de graven uit de noordelijke begraafplaats van Ayios Vasilios

Paleo-aktueel ◽  
2019 ◽  
pp. 11-22
Author(s):  
Youp van den Beld
Keyword(s):  
Building Materials ◽  
Low Cost ◽  
Human Interaction ◽  
Construction Process ◽  
Architectural Analysis ◽  
The North ◽  
Labour Costs ◽  
The Creation ◽  
Architectural Energetics ◽  
Architectural Unit

Building on yourself to build for another: An architectural analysis of the graves from the North Cemetery of Ayios Vasilios. For this paper I have systematically examined the construction process of the tombs from the North Cemetery of Ayios Vasilios (Laconia, Greece) using a method called architectural energetics. This method of analysis translates an architectural unit into labour costs. The calculated workhours have been combined with a precedence diagram in order to closely examine the level of human interaction, rather than to just compare labour costs. The study concludes that the transportation of the building materials that were used for the tombs was the most labour-intensive task. It also concludes that the materials that came from farthest away seem to have played the biggest role in the creation of meaning in the funerary rituals. Furthermore, two distinct strategies of construction were identified, one that focussed on scale, using low-cost materials that would have been locally available, and one that focussed on elaboration, using high-cost materials that must have come from elsewhere. The high-cost materials are theorized to have been involved in the creation of social networks among groups.

Intelligent product manuals

2000 ◽  
Vol 214 (5) ◽  
pp. 411-419 ◽  
Author(s):  
D T Pham ◽  
S S Dimov ◽  
B J Peat
Keyword(s):  
Selection Criteria ◽  
Low Cost ◽  
General Design ◽  
Design Considerations ◽  
Knowledge Based ◽  
Operation And Maintenance ◽  
Support Costs ◽  
Intelligent Product ◽  
The Creation ◽  
Active Assistance

Intelligent product manuals are designed to allow product users to utilize a product as easily, effectively and with as little additional care as possible while minimizing support costs for manufacturers and suppliers. It is first shown how intelligent product manuals address these objectives by utilizing electronic, multimedia and knowledge-based technologies to provide active assistance to the user of the product during tasks such as installation, operation and maintenance. An architecture for the creation and deployment of an intelligent product manual is then proposed and general design considerations are outlined. Finally, four implementation approaches, based on XML, SGML, HTML and PDF technologies, are compared against a set of selection criteria. It is concluded that simple, low-cost solutions are available, which can provide significant benefits for appropriate businesses, including smaller companies.

Is Culture Subjective? On the Issue of Russian Culturology Tradition

2021 ◽  
Vol 64 ◽  
pp. 69-76
Author(s):  
Nikolai A. Shchipkov
Keyword(s):  
Cultural Tradition ◽  
Research Fields ◽  
History Of ◽  
The Creation

The article examines the main stages of the history of the ideological development of the preconditions of the culturological methodology. The views of N.Ya. Danilevsky and A.N. Veselovsky on the concept “culture” are considered. The article describes the early Soviet experiments of the 1920s regarding the creation of new humanitarian research fields on the example of the GAHN. The main goal of the article is to trace the historical predecessors of the original attitude to the concept of “culture” within the framework of the Russian philosophical and cultural tradition.

scholarly journals Self-Propulsion Strategies for Artificial Cell-Like Compartments

Nanomaterials ◽  
2019 ◽  
Vol 9 (12) ◽  
pp. 1680 ◽  
Author(s):  
Ibon Santiago ◽  
Friedrich C. Simmel
Keyword(s):  
Synthetic Biology ◽  
Biomedical Engineering ◽  
Recent Progress ◽  
Emulsion Droplet ◽  
Artificial Cells ◽  
Droplet Motion ◽  
Active Particles ◽  
Artificial Cell ◽  
Catalytically Active ◽  
A Current

Reconstitution of life-like properties in artificial cells is a current research frontier in synthetic biology. Mimicking metabolism, growth, and sensing are active areas of investigation; however, achieving motility and directional taxis are also challenging in the context of artificial cells. To tackle this problem, recent progress has been made that leverages the tools of active matter physics in synthetic biology. This review surveys the most significant achievements in designing motile cell-like compartments. In this context, strategies for self-propulsion are summarized, including, compartmentalization of catalytically active particles, phoretic propulsion of vesicles and emulsion droplet motion driven by Marangoni flows. This work showcases how the realization of motile protocells may impact biomedical engineering while also aiming at answering fundamental questions in locomotion of prebiotic cells.

scholarly journals Biosensing and Actuation—Platforms Coupling Body Input-Output Modalities for Affective Technologies

Sensors ◽  
2020 ◽  
Vol 20 (21) ◽  
pp. 5968
Author(s):  
Miquel Alfaras ◽  
William Primett ◽  
Muhammad Umair ◽  
Charles Windlin ◽  
Pavel Karpashevich ◽  
...  
Keyword(s):  
Low Cost ◽  
Body Awareness ◽  
Health Condition ◽  
The Body ◽  
First Person ◽  
Self Awareness ◽  
Affective Experience ◽  
Health Domains ◽  
The Creation ◽  
Feedback Modalities

Research in the use of ubiquitous technologies, tracking systems and wearables within mental health domains is on the rise. In recent years, affective technologies have gained traction and garnered the interest of interdisciplinary fields as the research on such technologies matured. However, while the role of movement and bodily experience to affective experience is well-established, how to best address movement and engagement beyond measuring cues and signals in technology-driven interactions has been unclear. In a joint industry-academia effort, we aim to remodel how affective technologies can help address body and emotional self-awareness. We present an overview of biosignals that have become standard in low-cost physiological monitoring and show how these can be matched with methods and engagements used by interaction designers skilled in designing for bodily engagement and aesthetic experiences. Taking both strands of work together offers unprecedented design opportunities that inspire further research. Through first-person soma design, an approach that draws upon the designer’s felt experience and puts the sentient body at the forefront, we outline a comprehensive work for the creation of novel interactions in the form of couplings that combine biosensing and body feedback modalities of relevance to affective health. These couplings lie within the creation of design toolkits that have the potential to render rich embodied interactions to the designer/user. As a result we introduce the concept of “orchestration”. By orchestration, we refer to the design of the overall interaction: coupling sensors to actuation of relevance to the affective experience; initiating and closing the interaction; habituating; helping improve on the users’ body awareness and engagement with emotional experiences; soothing, calming, or energising, depending on the affective health condition and the intentions of the designer. Through the creation of a range of prototypes and couplings we elicited requirements on broader orchestration mechanisms. First-person soma design lets researchers look afresh at biosignals that, when experienced through the body, are called to reshape affective technologies with novel ways to interpret biodata, feel it, understand it and reflect upon our bodies.

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