scholarly journals Integration of Ultra-Low Volume Pneumatic Microfluidics with a Three-Dimensional Electrode Network for On-Chip Biochemical Sensing

Micromachines ◽  
2021 ◽  
Vol 12 (7) ◽  
pp. 762
Author(s):  
Saurabh Tomar ◽  
Charlotte Lasne ◽  
Sylvain Barraud ◽  
Thomas Ernst ◽  
Carlotta Guiducci
Keyword(s):  
Field Effect Transistors ◽  
Scale Up ◽  
Three Dimensional ◽  
Microfluidic Channel ◽  
Reference Electrode ◽  
Open Circuit ◽  
Cmos Scaling ◽  
Pneumatic Valves ◽  
On Chip ◽  
The Stability

This paper reports a novel miniaturized pseudo reference electrode (RE) design for biasing Ion Sensitive Field Effect Transistors (ISFETs). It eliminates the need for post-CMOS processing and can scale up in numbers with the CMOS scaling. The presented design employs silane-mediated transfer of patterned gold electrode lines onto PDMS microfluidics such that the gold conformally coats the inside of microfluidic channel. Access to this electrode network is made possible by using “through-PDMS-vias” (TPV), which consist of high metal-coated SU-8 pillars manufactured by a novel process that employs a patterned positive resist layer as SU-8 adhesion depressor. When integrated with pneumatic valves, TPV and pseudo-RE network were able to bias 1.5 nanoliters (nL) of isolated electrolyte volumes. We present a detailed characterization of our pseudo-RE design demonstrating ISFET operation and its DC characterization. The stability of pseudo-RE is investigated by measuring open circuit potential (OCP) against a commercial Ag/AgCl reference electrode.

scholarly journals Selective Retrieval of Individual Cells from Microfluidic Arrays Combining Dielectrophoretic Force and Directed Hydrodynamic Flow

Micromachines ◽  
2020 ◽  
Vol 11 (3) ◽  
pp. 322
Author(s):  
Pierre-Emmanuel Thiriet ◽  
Joern Pezoldt ◽  
Gabriele Gambardella ◽  
Kevin Keim ◽  
Bart Deplancke ◽  
...  
Keyword(s):  
Single Cell ◽  
Single Cells ◽  
Three Dimensional ◽  
Microfluidic Channel ◽  
Microfluidic System ◽  
Transcriptional Analysis ◽  
Molecular Profile ◽  
Cell Phenotype ◽  
Pneumatic Valves ◽  
On Chip

Hydrodynamic-based microfluidic platforms enable single-cell arraying and analysis over time. Despite the advantages of established microfluidic systems, long-term analysis and proliferation of cells selected in such devices require off-chip recovery of cells as well as an investigation of on-chip analysis on cell phenotype, requirements still largely unmet. Here, we introduce a device for single-cell isolation, selective retrieval and off-chip recovery. To this end, singularly addressable three-dimensional electrodes are embedded within a microfluidic channel, allowing the selective release of single cells from their trapping site through application of a negative dielectrophoretic (DEP) force. Selective capture and release are carried out in standard culture medium and cells can be subsequently mitigated towards a recovery well using micro-engineered hybrid SU-8/PDMS pneumatic valves. Importantly, transcriptional analysis of recovered cells revealed only marginal alteration of their molecular profile upon DEP application, underscored by minor transcriptional changes induced upon injection into the microfluidic device. Therefore, the established microfluidic system combining targeted DEP manipulation with downstream hydrodynamic coordination of single cells provides a powerful means to handle and manipulate individual cells within one device.

scholarly journals Fabrication of a Pneumatic Microparticle Concentrator

Micromachines ◽  
2019 ◽  
Vol 11 (1) ◽  
pp. 40
Author(s):  
Jun Ho Jang ◽  
Ok Chan Jeong
Keyword(s):  
Three Dimensional ◽  
Fem Simulation ◽  
Particle Diameter ◽  
Microfluidic Channel ◽  
Solid Particles ◽  
Working Fluid ◽  
Operating Pressure ◽  
Fluidic Channel ◽  
Outlet Port ◽  
Pneumatic Valves

We developed a microfluidic platform employing (normally open) pneumatic valves for particle concentration. The device features a three-dimensional network with a curved fluidic channel and three pneumatic valves (a sieve valve (Vs) that concentrates particles and two ON/OFF rubber-seal pneumatic valves that block the working fluid). Double-sided replication employing polydimethylsiloxane (PDMS) was used to fabricate the network, channel, and chamber. Particles were blocked by deformation of the Vs diaphragm, and then accumulated in the curved microfluidic channel. The working fluid was discharged via operation of the two ON/OFF valves. After concentration, particles were released to an outlet port. The Vs pressure required to block solid particles varying in diameter was determined based on the height of the curved microchannel and a finite element method (FEM) simulation of Vs diaphragm displacement. Our method was verified according to the temporal response of the fluid flow rate controlled by the pneumatic valves. Furthermore, all particles with various diameters were successfully blocked, accumulated, and released. The operating pressure, time required for concentration, and concentration ratio were dependent on the particle diameter. The estimated concentration percentage of 24.9 µm diameter polystyrene particles was about 3.82% for 20 min of operation.

scholarly journals On-Chip Fabrication of Cell-Attached Microstructures using Photo-Cross-Linkable Biodegradable Hydrogel

2020 ◽  
Vol 11 (1) ◽  
pp. 18 ◽  
Author(s):  
Masaru Takeuchi ◽  
Taro Kozuka ◽  
Eunhye Kim ◽  
Akihiko Ichikawa ◽  
Yasuhisa Hasegawa ◽  
...  
Keyword(s):  
Cell Culture ◽  
Microfluidic Chip ◽  
Three Dimensional ◽  
Microfluidic Channel ◽  
Hydrophobic Coating ◽  
Cell Structures ◽  
Rat Liver Cells ◽  
Biodegradable Hydrogel ◽  
On Chip

We developed a procedure for fabricating movable biological cell structures using biodegradable materials on a microfluidic chip. A photo-cross-linkable biodegradable hydrogel gelatin methacrylate (GelMA) was used to fabricate arbitrary microstructure shapes under a microscope using patterned ultraviolet light. The GelMA microstructures were movable inside the microfluidic channel after applying a hydrophobic coating material. The fabricated microstructures were self-assembled inside the microfluidic chip using our method of fluid forcing. The synthesis procedure of GelMA was optimized by changing the dialysis temperature, which kept the GelMA at a suitable pH for cell culture. RLC-18 rat liver cells (Riken BioResource Research Center, Tsukuba, Japan) were cultured inside the GelMA and on the GelMA microstructures to check cell growth. The cells were then stretched for 1 day in the cell culture and grew well on the GelMA microstructures. However, they did not grow well inside the GelMA microstructures. The GelMA microstructures were partially dissolved after 4 days of cell culture because of their biodegradability after the cells were placed on the microstructures. The results indicated that the proposed procedure used to fabricate cell structures using GelMA can be used as a building block to assemble three-dimensional tissue-like cell structures in vitro inside microfluidic devices.

Organic Three-dimensional Field-effect Transistors

2008 ◽  
Author(s):  
M. Uno ◽  
I. Doi ◽  
K. Takimiya ◽  
Jun Takeya
Keyword(s):  
Field Effect ◽  
Field Effect Transistors ◽  
Three Dimensional

A Liquid Metal Flow Between Two Coaxial Cylinders System

2019 ◽  
Vol 11 (1) ◽  
pp. 79-86
Author(s):  
Abdelkrim Merah ◽  
Ridha Kelaiaia ◽  
Faiza Mokhtari
Keyword(s):  
Couette Flow ◽  
Metal Flow ◽  
Three Dimensional ◽  
Liquid Sodium ◽  
Taylor Vortex ◽  
Turbulent Regime ◽  
Coaxial Cylinders ◽  
Concentric Cylinders ◽  
Ideal Tool ◽  
The Stability

Abstract The Taylor-Couette flow between two rotating coaxial cylinders remains an ideal tool for understanding the mechanism of the transition from laminar to turbulent regime in rotating flow for the scientific community. We present for different Taylor numbers a set of three-dimensional numerical investigations of the stability and transition from Couette flow to Taylor vortex regime of a viscous incompressible fluid (liquid sodium) between two concentric cylinders with the inner one rotating and the outer one at rest. We seek the onset of the first instability and we compare the obtained results for different velocity rates. We calculate the corresponding Taylor number in order to show its effect on flow patterns and pressure field.

Diagnostic System of Gravitational Solid Flow Based on Weight and Accelerometer Signal Analysis Using Wireless Data Transmission Technology

2012 ◽  
Vol 17 (4) ◽  
pp. 319-326 ◽  
Author(s):  
Zbigniew Chaniecki ◽  
Krzysztof Grudzień ◽  
Tomasz Jaworski ◽  
Grzegorz Rybak ◽  
Andrzej Romanowski ◽  
...  
Keyword(s):  
Signal Analysis ◽  
Scale Up ◽  
Three Dimensional ◽  
Diagnostic System ◽  
Wireless Data ◽  
Stick Slip ◽  
Wireless Data Transmission ◽  
Accelerometer Signal ◽  
Flow Investigation ◽  
Material Information

Abstract The paper presents results of the scale-up silo flow investigation in based on accelerometer signal analysis and Wi-Fi transmission, performed in distributed laboratory environment. Prepared, by the authors, a set of 8 accelerometers allows to measure a three-dimensional acceleration vector. The accelerometers were located outside silo, on its perimeter. The accelerometers signal changes allowed to analyze dynamic behavior of solid (vibrations/pulsations) at silo wall during discharging process. These dynamic effects are caused by stick-slip friction between the wall and the granular material. Information about the material pulsations and vibrations is crucial for monitoring the interaction between silo construction and particle during flow. Additionally such spatial position of accelerometers sensor allowed to collect information about nonsymmetrical flow inside silo.

Constructive Optimisation of the Propeller Type Mixing Devices Using the Virtual and Rapid Prototyping

2017 ◽  
Vol 68 (3) ◽  
pp. 453-458 ◽  
Author(s):  
Daniel Besnea ◽  
Alina Spanu ◽  
Iuliana Marlena Prodea ◽  
Gheorghita Tomescu ◽  
Iolanda Constanta Panait
Keyword(s):  
Rapid Prototyping ◽  
Low Cost ◽  
Scale Up ◽  
Three Dimensional ◽  
Rapid Identification ◽  
Multidisciplinary Optimization ◽  
External Diameter ◽  
Process Industries ◽  
Parametric Cad ◽  
Shaft Torque

The paper points out the advantages of rapid prototyping for improving the performances/constructive optimization of mixing devices used in process industries, here exemplified to propeller types ones. The multidisciplinary optimization of the propeller profile affords its design using parametric CAD methods. Starting from the mathematical curve equations proposed for the blade profile, it was determined its three-dimensional virtual model. The challenge has been focused on the variation of propeller pitch and external diameter. Three dimensional ranges were manufactured using the additive manufacturing process with Marker Boot 3D printer. The mixing performances were tested on the mixing equipment measuring the minimum rotational speed and the correspondent shaft torque for complete suspension achieved for each of the three models. The virtual and rapid prototyping method is newly proposed by the authors to obtain the basic data for scale up of the mixing systems, in the case of flexible production (of low quantities), in which both the nature and concentration of the constituents in the final product varies often. It is an efficient and low cost method for the rapid identification of the optimal mixing device configuration, which contributes to the costs reduction and to the growing of the output.

Handheld Laser Scanner Research

2019 ◽  
Vol 952 (10) ◽  
pp. 47-54
Author(s):  
A.V. Komissarov ◽  
A.V. Remizov ◽  
M.M. Shlyakhova ◽  
K.K. Yambaev
Keyword(s):  
Point Cloud ◽  
Three Dimensional ◽  
Laser Scanner ◽  
Test Site ◽  
Optical Systems ◽  
Advantages And Disadvantages ◽  
Site Survey ◽  
Laser Scanners ◽  
Three Dimensional Models ◽  
The Stability

The authors consider hand-held laser scanners, as a new photogrammetric tool for obtaining three-dimensional models of objects. The principle of their work and the newest optical systems based on various sensors measuring the depth of space are described in detail. The method of simultaneous navigation and mapping (SLAM) used for combining single scans into point cloud is outlined. The formulated tasks and methods for performing studies of the DotProduct (USA) hand-held laser scanner DPI?8X based on a test site survey are presented. The accuracy requirements for determining the coordinates of polygon points are given. The essence of the performed experimental research of the DPI?8X scanner is described, including scanning of a test object at various scanner distances, shooting a test polygon from various scanner positions and building point cloud, repeatedly shooting the same area of the polygon to check the stability of the scanner. The data on the assessment of accuracy and analysis of research results are given. Fields of applying hand-held laser scanners, their advantages and disadvantages are identified.

scholarly journals Polarization-sensitive photodetectors based on three-dimensional molybdenum disulfide (MoS2) field-effect transistors

Nanophotonics ◽  
2020 ◽  
Vol 9 (16) ◽  
pp. 4719-4728
Author(s):  
Tao Deng ◽  
Shasha Li ◽  
Yuning Li ◽  
Yang Zhang ◽  
Jingye Sun ◽  
...  
Keyword(s):  
Molybdenum Disulfide ◽  
Field Effect ◽  
High Performance ◽  
Field Effect Transistors ◽  
Three Dimensional ◽  
Polarized Light ◽  
Optical Field ◽  
Two Dimensional ◽  
Polarization Ratio ◽  
Two Dimensional Materials

AbstractThe molybdenum disulfide (MoS2)-based photodetectors are facing two challenges: the insensitivity to polarized light and the low photoresponsivity. Herein, three-dimensional (3D) field-effect transistors (FETs) based on monolayer MoS2 were fabricated by applying a self–rolled-up technique. The unique microtubular structure makes 3D MoS2 FETs become polarization sensitive. Moreover, the microtubular structure not only offers a natural resonant microcavity to enhance the optical field inside but also increases the light-MoS2 interaction area, resulting in a higher photoresponsivity. Photoresponsivities as high as 23.8 and 2.9 A/W at 395 and 660 nm, respectively, and a comparable polarization ratio of 1.64 were obtained. The fabrication technique of the 3D MoS2 FET could be transferred to other two-dimensional materials, which is very promising for high-performance polarization-sensitive optical and optoelectronic applications.

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