Self-Renewal and Differentiation of Adipose-Derived Stem Cells (ADSCs) Stimulated by Multi-Axial Tensile Strain in a Pneumatic Microdevice

Adipose-derived stem cells (ADSCs) were suggested for treating degenerative osteoarthritis, suppressing inflammatory responses, and repairing damaged soft tissues. Moreover, the ADSCs have the potential to undergo self-renewal and differentiate into bone, tendon, cartilage, and ligament. Recently, investigation of the self-renewal and differentiation of the ADSCs has become an attractive area. In this work, a pneumatic microdevice has been developed to study the gene expression of the ADSCs after the stimulation of multi-axial tensile strain. The ADSCs were cultured on the microdevice and experienced multi-axial tensile strain during a three-day culture course. Self-renewal and differentiation abilities were investigated by mRNA expressions of NANOG, sex determining region Y-box 2 (SOX2), octamer-binding transcription factor 4 (OCT4), sex determining region Y-box9 (SOX9), peroxisome proliferator-activated receptor gamma (PPAR-γ), and runt-related transcription factor 2 (RUNX2). The result showed that the genes related self-renewal were significantly up-regulated after the tensile stimulation. Higher proliferation ratio of the ADSCs was also shown by cell viability assay. The microdevice provides a promising platform for cell-based study under mechanical tensile stimulation.

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Self-Renewal and Differentiation of Adipose-Derived Stem Cells (ADSCs) Stimulated by Multi-Axial Tensile Strain in a Pneumatic Microdevice

10.20944/preprints201809.0503.v1 ◽

2018 ◽

Author(s):

Chih-Hao Chiu ◽

Yun-Wen Tong ◽

Wen-Ling Yeh ◽

Kin Fong Lei ◽

Alvin Chao-Yu Chen

Keyword(s):

Stem Cells ◽

Tensile Strain ◽

Soft Tissues ◽

Inflammatory Responses ◽

Adipose Derived Stem Cells ◽

Cell Viability Assay ◽

Self Renewal ◽

Viability Assay ◽

Axial Tensile ◽

Mechanical Tensile

Adipose-derived stem cells (ADSCs) were suggested for treating degenerative osteoarthritis, suppressing inflammatory responses, and repairing damaged soft tissues. Moreover, the ADSCs have the potential to undergo self-renewal and differentiate into bone, tendon, cartilage, and ligament. Recently, investigation of the self-renewal and differentiation of the ADSCs becomes an attractive area. In this work, a pneumatic microdevice has been developed to study the gene expression of the ADSCs after the stimulation of multi-axial tensile strain. The ADSCs were cultured on the microdevice and experienced multi-axial tensile strain during a 3-day culture course. Self-renewal and differentiation abilities were investigated by mRNA expressions of NANOG, SOX2, OCT4, SOX9, PPAR-γ, and RUNX2. The result showed that the genes related self-renewal were significantly up-regulated after the tensile stimulation. Higher proliferation ratio of the ADSCs was also shown by cell viability assay. The microdevice provides a promising platform for cell-based study under mechanical tensile stimulation.

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Piceatannol Is Superior to Resveratrol at Suppressing Adipogenesis in Human Visceral Adipose-Derived Stem Cells

Plants ◽

10.3390/plants10020366 ◽

2021 ◽

Vol 10 (2) ◽

pp. 366

Author(s):

In Sil Park ◽

Youngjin Han ◽

HyunA Jo ◽

Ki Won Lee ◽

Yong Sang Song

Keyword(s):

Stem Cells ◽

Metabolic Diseases ◽

Binding Protein ◽

Visceral Obesity ◽

Peroxisome Proliferator ◽

Adipose Derived Stem Cells ◽

Fatty Acid Binding ◽

Chemical Structures ◽

Visceral Adipose ◽

Adipogenic Effect

Resveratrol (3,4′,5-trans-trihydroxystilbene) and piceatannol (3,3′,4′,5-trans-tetraphydroxystilbene) are major stilbene compounds that are predominantly present in various natural foods, such as berries and fruits. Both phytochemical compounds are consumed as dietary supplements to prevent various metabolic diseases and for their anti-aging properties. Adipose-derived stem cells from human visceral adipose tissue (vASCs) are a useful in vitro model for evaluating their adipogenic effect. Treatment with resveratrol and piceatannol significantly inhibited lipid accumulation in vASCs. Their effective concentrations were 5, 10, and 20 μM for inhibiting adipogenesis of vASCs. Interestingly, despite the similar chemical structures of the two compounds, piceatannol showed a higher anti-adipogenic effect at 20 μM than resveratrol in vASCs. Moreover, the inhibitory capacity of lipid droplet generation was higher for piceatannol at 20 μM than that of resveratrol. Piceatannol significantly attenuated the expression level of adipogenic markers (e.g., CCAAT/enhanced binding protein α (C/EBPα), peroxisome proliferator-activated receptor γ (PPARγ), and adipocyte fatty acid binding protein (aP2)) compared to resveratrol at the mRNA and protein levels. These results suggest that piceatannol is a superior anti-adipogenic compound compared to resveratrol in the vASC model of visceral obesity.

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Enhanced Therapeutic Potential of the Secretome Released from Adipose-Derived Stem Cells by PGC-1α-Driven Upregulation of Mitochondrial Proliferation

International Journal of Molecular Sciences ◽

10.3390/ijms20225589 ◽

2019 ◽

Vol 20 (22) ◽

pp. 5589

Author(s):

Jaeim Lee ◽

Ok-Hee Kim ◽

Sang Chul Lee ◽

Kee-Hwan Kim ◽

Jin Sun Shin ◽

...

Keyword(s):

Stem Cells ◽

Liver Injury ◽

Therapeutic Potential ◽

Tissue Expression ◽

In Vitro Models ◽

Peroxisome Proliferator ◽

Adipose Derived Stem Cells ◽

Peroxisome Proliferator Activated Receptor

Peroxisome proliferator activated receptor λ coactivator 1α (PGC-1α) is a potent regulator of mitochondrial biogenesis and energy metabolism. In this study, we investigated the therapeutic potential of the secretome released from the adipose-derived stem cells (ASCs) transfected with PGC-1α (PGC-secretome). We first generated PGC-1α-overexpressing ASCs by transfecting ASCs with the plasmids harboring the gene encoding PGC-1α. Secretory materials released from PGC-1α-overexpressing ASCs were collected and their therapeutic potential was determined using in vitro (thioacetamide (TAA)-treated AML12 cells) and in vivo (70% partial hepatectomized mice) models of liver injury. In the TAA-treated AML12 cells, the PGC-secretome significantly increased cell viability, promoted expression of proliferation-related markers, such as PCNA and p-STAT, and significantly reduced the levels of reactive oxygen species (ROS). In the mice, PGC-secretome injections significantly increased liver tissue expression of proliferation-related markers more than normal secretome injections did (p < 0.05). We demonstrated that the PGC-secretome does not only have higher antioxidant and anti-inflammatory properties, but also has the potential of significantly enhancing liver regeneration in both in vivo and in vitro models of liver injury. Thus, reinforcing the mitochondrial antioxidant potential by transfecting ASCs with PGC-1α could be one of the effective strategies to enhance the therapeutic potential of ASCs.

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Unravelling evolution of Nanog, the key transcription factor involved in self-renewal of undifferentiated embryonic stem cells, by pattern recognition in nucleotide and tandem repeats characteristics

Gene ◽

10.1016/j.gene.2015.12.023 ◽

2016 ◽

Vol 578 (2) ◽

pp. 194-204 ◽

Cited By ~ 12

Author(s):

Maryam Pashaiasl ◽

Khodadad Khodadadi ◽

Amir Hossein Kayvanjoo ◽

Roghiyeh Pashaei-asl ◽

Esmaeil Ebrahimie ◽

...

Keyword(s):

Stem Cells ◽

Pattern Recognition ◽

Transcription Factor ◽

Embryonic Stem Cells ◽

Tandem Repeats ◽

Embryonic Stem ◽

Self Renewal

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Hypoxia inducible factor down-regulation, cancer and cancer stem cells (CSCs): ongoing success stories

MedChemComm ◽

10.1039/c6md00432f ◽

2017 ◽

Vol 8 (1) ◽

pp. 21-52 ◽

Cited By ~ 8

Author(s):

Anthony R. Martin ◽

Cyril Ronco ◽

Luc Demange ◽

Rachid Benhida

Keyword(s):

Stem Cells ◽

Transcription Factor ◽

Cancer Stem Cells ◽

Hypoxia Inducible Factor ◽

Large Set ◽

Down Regulation ◽

Self Renewal ◽

Hypoxia Inducible Factor 1 ◽

Success Stories ◽

Tumour Vascularization

In cancers, hypoxia inducible factor 1 (HIF-1) is an over-expressed transcription factor, which regulates a large set of genes involved in tumour vascularization, metastases, and cancer stem cells (CSCs) formation and self-renewal.

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Pdcd4 restrains the self-renewal and white-to-beige transdifferentiation of adipose-derived stem cells

Cell Death and Disease ◽

10.1038/cddis.2016.75 ◽

2016 ◽

Vol 7 (3) ◽

pp. e2169-e2169 ◽

Cited By ~ 7

Author(s):

Y Bai ◽

Q Shang ◽

H Zhao ◽

Z Pan ◽

C Guo ◽

...

Keyword(s):

Stem Cells ◽

The Self ◽

Adipose Derived Stem Cells ◽

Self Renewal

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Fgf and Esrrb integrate epigenetic and transcriptional networks that regulate self-renewal of trophoblast stem cells

Nature Communications ◽

10.1038/ncomms8776 ◽

2015 ◽

Vol 6 (1) ◽

Cited By ~ 54

Author(s):

Paulina A. Latos ◽

Angela Goncalves ◽

David Oxley ◽

Hisham Mohammed ◽

Ernest Turro ◽

...

Keyword(s):

Stem Cells ◽

Transcription Factor ◽

Transcription Factors ◽

Rna Polymerase Ii ◽

Es Cells ◽

Embryonic Stem ◽

Transcriptional Networks ◽

Self Renewal ◽

Downstream Target ◽

Trophoblast Stem

Abstract Esrrb (oestrogen-related receptor beta) is a transcription factor implicated in embryonic stem (ES) cell self-renewal, yet its knockout causes intrauterine lethality due to defects in trophoblast development. Here we show that in trophoblast stem (TS) cells, Esrrb is a downstream target of fibroblast growth factor (Fgf) signalling and is critical to drive TS cell self-renewal. In contrast to its occupancy of pluripotency-associated loci in ES cells, Esrrb sustains the stemness of TS cells by direct binding and regulation of TS cell-specific transcription factors including Elf5 and Eomes. To elucidate the mechanisms whereby Esrrb controls the expression of its targets, we characterized its TS cell-specific interactome using mass spectrometry. Unlike in ES cells, Esrrb interacts in TS cells with the histone demethylase Lsd1 and with the RNA Polymerase II-associated Integrator complex. Our findings provide new insights into both the general and context-dependent wiring of transcription factor networks in stem cells by master transcription factors.

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