scholarly journals The Darkest Place Is under the Candlestick-Healthy Urogenital Tract as a Source of Worldwide Disseminated Extraintestinal Pathogenic Escherichia coli Lineages

2021 ◽  
Vol 10 (1) ◽  
pp. 27
Author(s):  
Magdalena Ksiezarek ◽  
Ângela Novais ◽  
Luísa Peixe
Keyword(s):  
Escherichia Coli ◽  
Population Diversity ◽  
Sample Type ◽  
Antibiotic Resistant ◽  
E Coli ◽  
Pathogenic Escherichia Coli ◽  
Amoxicillin Clavulanate ◽  
Urinary Microbiome ◽  
Close Relatedness ◽  
Sequence Types

Since the discovery of the urinary microbiome, including the identification of Escherichia coli in healthy hosts, its involvement in UTI development has been a subject of high interest. We explored the population diversity and antimicrobial resistance of E. coli (n = 22) in the urogenital microbiome of ten asymptomatic women (representing 50% of the sample tested). We evaluated their genomic relationship with extraintestinal pathogenic E. coli (ExPEC) strains from healthy and diseased hosts, including the ST131 lineage. E. coli prevalence was higher in vaginal samples than in urine samples, and occasionally different lineages were observed in the same individual. Furthermore, B2 was the most frequent phylogenetic group, with the most strains classified as ExPEC. Resistance to antibiotics of therapeutic relevance (e.g., amoxicillin-clavulanate conferred by blaTEM-30) was observed in ExPEC widespread lineages sequence types (ST) 127, ST131, and ST73 and ST95 clonal complexes. Phylogenomics of ST131 and other ExPEC lineages revealed close relatedness with strains from gastrointestinal tract and diseased host. These findings demonstrate that healthy urogenital microbiome is a source of potentially pathogenic and antibiotic resistant E. coli strains, including those causing UTI, e.g., ST131. Importantly, diverse E. coli lineages can be observed per individual and urogenital sample type which is relevant for future studies screening for this uropathogen.

scholarly journals The darkest place is under the candlestick - healthy urogenital tract as a source of UTI-related Escherichia coli lineages

2021 ◽  
Author(s):  
Magdalena Ksiezarek ◽  
Angela Novais ◽  
Luisa Peixe
Keyword(s):  
Escherichia Coli ◽  
Population Diversity ◽  
Genomic Relationship ◽  
Antibiotic Resistant ◽  
E Coli ◽  
Recurrent Uti ◽  
Urinary Microbiome ◽  
Close Relatedness ◽  
Sequence Types ◽  
Asymptomatic Women

Since the discovery of the urinary microbiome, including identification of Escherichia coli in healthy host, its involvement in UTI development is a subject of high interest. We explored population diversity and antimicrobial resistance of E. coli from urogenital microbiome of asymptomatic and recurrent UTI (rUTI) women. We also evaluated the genomic relationship between extraintestinal pathogenic E. coli (ExPEC) strains from healthy and diseased host, particularly of the ST131 lineage. E. coli was highly prevalent in asymptomatic women (48%) with slightly higher prevalence in vaginal samples comparing to urine, and occasionally with multiclonal population in the same individual. B2 was the most frequent phylogenetic group, with most strains classified as ExPEC. We demonstrated that virulence associated genes profile does not allow to distinguish strains isolated from healthy and rUTI host. We identified E. coli widespread lineages e.g., sequence types (ST) 127, ST131 (asymptomatic cohort) and ST73, ST131 (rUTI), frequently resistant to at least one antibiotic. Phylogenomics of ST131 and other ExPEC lineages revealed close relatedness between healthy and diseased host. These findings demonstrate that healthy urogenital microbiome is a source of potentially pathogenic and antibiotic resistant E. coli strains, including globally spread E. coli lineages causing UTI including ST131.

scholarly journals Population genomics and antimicrobial resistance dynamics of Escherichia coli in wastewater and river environments

2021 ◽  
Vol 4 (1) ◽  
Author(s):  
Jose F. Delgado-Blas ◽  
Cristina M. Ovejero ◽  
Sophia David ◽  
Natalia Montero ◽  
William Calero-Caceres ◽  
...  
Keyword(s):  
Escherichia Coli ◽  
Antimicrobial Resistance ◽  
Population Genomics ◽  
Population Diversity ◽  
Genomic Diversity ◽  
Resistant Bacteria ◽  
Aquatic Environments ◽  
E Coli ◽  
Water Ecosystems ◽  
Sequence Types

AbstractAquatic environments are key niches for the emergence, evolution and dissemination of antimicrobial resistance. However, the population diversity and the genetic elements that drive the dynamics of resistant bacteria in different aquatic environments are still largely unknown. The aim of this study was to understand the population genomics and evolutionary events of Escherichia coli resistant to clinically important antibiotics including aminoglycosides, in anthropogenic and natural water ecosystems. Here we show that less different E. coli sequence types (STs) are identified in wastewater than in rivers, albeit more resistant to antibiotics, and with significantly more plasmids/cell (6.36 vs 3.72). However, the genomic diversity within E. coli STs in both aquatic environments is similar. Wastewater environments favor the selection of conserved chromosomal structures associated with diverse flexible plasmids, unraveling promiscuous interplasmidic resistance genes flux. On the contrary, the key driver for river E. coli adaptation is a mutable chromosome along with few plasmid types shared between diverse STs harboring a limited resistance gene content.

scholarly journals Synergism of the Combination of Traditional Antibiotics and Novel Phenolic Compounds against Escherichia coli

Pathogens ◽  
2020 ◽  
Vol 9 (10) ◽  
pp. 811
Author(s):  
Md. Akil Hossain ◽  
Hae-Chul Park ◽  
Sung-Won Park ◽  
Seung-Chun Park ◽  
Min-Goo Seo ◽  
...  
Keyword(s):  
Escherichia Coli ◽  
Rattus Norvegicus ◽  
Inhibitory Concentration ◽  
Epicatechin Gallate ◽  
Antibiotic Resistant ◽  
E Coli ◽  
Pathogenic Escherichia Coli ◽  
Resistant Strains ◽  
Novel Approaches ◽  
Rapid Emergence

Pathogenic Escherichia coli (E. coli)-associated infections are becoming difficult to treat because of the rapid emergence of antibiotic-resistant strains. Novel approaches are required to prevent the progression of resistance and to extend the lifespan of existing antibiotics. This study was designed to improve the effectiveness of traditional antibiotics against E. coli using a combination of the gallic acid (GA), hamamelitannin, epicatechin gallate, epigallocatechin, and epicatechin. The fractional inhibitory concentration index (FICI) of each of the phenolic compound-antibiotic combinations against E. coli was ascertained. Considering the clinical significance and FICI, two combinations (hamamelitannin-erythromycin and GA-ampicillin) were evaluated for their impact on certain virulence factors of E. coli. Finally, the effects of hamamelitannin and GA on Rattus norvegicus (IEC-6) cell viability were investigated. The FICIs of the antibacterial combinations against E. coli were 0.281–1.008. The GA-ampicillin and hamamelitannin-erythromycin combinations more effectively prohibited the growth, biofilm viability, and swim and swarm motilities of E. coli than individual antibiotics. The concentration of hamamelitannin and GA required to reduce viability by 50% (IC50) in IEC-6 cells was 988.54 μM and 564.55 μM, correspondingly. GA-ampicillin and hamamelitannin-erythromycin may be potent combinations and promising candidates for eradicating pathogenic E. coli in humans and animals.

scholarly journals Global Extraintestinal Pathogenic Escherichia coli (ExPEC) Lineages

2019 ◽  
Vol 32 (3) ◽  
Author(s):  
Amee R. Manges ◽  
Hyun Min Geum ◽  
Alice Guo ◽  
Thaddeus J. Edens ◽  
Chad D. Fibke ◽  
...  
Keyword(s):  
Escherichia Coli ◽  
Surveillance Systems ◽  
Inclusion Criteria ◽  
Prevention Strategies ◽  
Content Type ◽  
E Coli ◽  
Treatment And Prevention ◽  
Pathogenic Escherichia Coli ◽  
Sequence Types ◽  
Study Inclusion

SUMMARY Extraintestinal pathogenic Escherichia coli (ExPEC) strains are responsible for a majority of human extraintestinal infections globally, resulting in enormous direct medical and social costs. ExPEC strains are comprised of many lineages, but only a subset is responsible for the vast majority of infections. Few systematic surveillance systems exist for ExPEC. To address this gap, we systematically reviewed and meta-analyzed 217 studies (1995 to 2018) that performed multilocus sequence typing or whole-genome sequencing to genotype E. coli recovered from extraintestinal infections or the gut. Twenty major ExPEC sequence types (STs) accounted for 85% of E. coli isolates from the included studies. ST131 was the most common ST from 2000 onwards, covering all geographic regions. Antimicrobial resistance-based isolate study inclusion criteria likely led to an overestimation and underestimation of some lineages. European and North American studies showed similar distributions of ExPEC STs, but Asian and African studies diverged. Epidemiology and population dynamics of ExPEC are complex; summary proportion for some STs varied over time (e.g., ST95), while other STs were constant (e.g., ST10). Persistence, adaptation, and predominance in the intestinal reservoir may drive ExPEC success. Systematic, unbiased tracking of predominant ExPEC lineages will direct research toward better treatment and prevention strategies for extraintestinal infections.

Molecular detection of multidrug and colistin-resistant Escherichia coli isolated from house flies in various environmental settings

2019 ◽  
Vol 14 (10) ◽  
pp. 847-858 ◽  
Author(s):  
Abdus Sobur ◽  
Zobayda F Haque ◽  
Abdullah AM Sabuj ◽  
Samina Ievy ◽  
AMM Taufiqur Rahman ◽  
...  
Keyword(s):  
Escherichia Coli ◽  
Multidrug Resistant ◽  
Diffusion Test ◽  
Isolation And Identification ◽  
Antibiotic Resistant ◽  
Disk Diffusion Test ◽  
House Flies ◽  
E Coli ◽  
Pathogenic Escherichia Coli ◽  
Antimicrobial Resistance Genes

Aim: To detect antibiotic-resistant pathogenic Escherichia coli in house flies captured from various environmental settings. Materials & methods: Isolation and identification of E. coli was performed based on culture and PCR. Antimicrobial susceptibility testing was conducted using disk diffusion test. Detection of virulence and antimicrobial resistance genes was carried out using PCR methods. Results: The prevalence of E. coli in flies was 61% with highest in dairy farms (70.67%) followed by food centers (65.33%), dustbins (64%) and area close to hospital (44%). The prevalence rates of tetA, tetB, ereA, CITM, SHV, mcr-3 and catA1 resistance determinants were 88.75, 48.52, 41.67, 37.17, 27.77, 18.37 and 14.81%, respectively. Conclusion: Multidrug-resistant pathogenic E. coli are widely distributed in house flies, which requires a one-health approach to delineate their control.

Detection of Virulence-Associated Genes in Escherichia coli O157 and Non-O157 Isolates from Beef Cattle, Humans, and Chickens

2008 ◽  
Vol 71 (9) ◽  
pp. 1774-1784 ◽  
Author(s):  
BRIGITTE LEFEBVRE ◽  
MOUSSA S. DIARRA ◽  
HÉLÈNE MOISAN ◽  
FRANÇOIS MALOUIN
Keyword(s):  
Escherichia Coli ◽  
Beef Cattle ◽  
Broiler Chickens ◽  
Animal Origin ◽  
Pathogenic Potential ◽  
Antibiotic Resistant ◽  
E Coli ◽  
Pathogenic Escherichia Coli ◽  
Type 3 Secretion System ◽  
Type 3

Food-producing animals can be reservoirs of pathogenic Escherichia coli strains that can induce diseases in animals or humans. Contamination of food by E. coli O157:H7 raises immediate concerns about public health, although it is not clear whether all E. coli O157 isolates of animal origin are equally harmful to humans. Inversely, the pathogenic potential of atypical E. coli O157 isolates and several non-O157 serotypes often is ignored. We used a DNA microarray capable of detecting a subset of 346 genes to compare the virulence-associated genes present in eight E. coli O157 isolates from human cases, 14 antibiotic-resistant and/or hypermutable E. coli O157 isolates from beef cattle, and four antibiotic-resistant, sorbitol-negative, non-O157 E. coli isolates from healthy broiler chickens. Hybridization on arrays (HOA) revealed that O157 isolates from beef cattle and humans were genetically distinct, although they possessed most of the same subset of virulence genes. HOA allowed discrimination between hypermutable and antibiotic-resistant O157 isolates from beef cattle based on hybridization results for the stx2 and ycgG genes (hypermutable) or ymfL, stx1, stx2, and hlyEavian genes (resistant). However, the absence of hybridization to gene yfdR characterized human isolates. HOA also revealed that an atypical sorbitol-fermenting bovine O157 isolate lacked some genes of the type 3 secretion system, plasmid pO157, and the stx1 and stx2 genes. This isolate had a particular pathotype (eaeAβ tirα espAα espBα espDα) not found in typical E. coli O157:H7. HOA revealed that some non-O157 E. coli isolates from healthy chickens carried genes responsible for salmochelin- and yersiniabactin-mediated iron uptake generally associated with pathogenic strains.

scholarly journals Pathogenic Escherichia coli Strains Recovered from Selected Aquatic Resources in the Eastern Cape, South Africa, and Its Significance to Public Health

2018 ◽  
Vol 15 (7) ◽  
pp. 1506 ◽  
Author(s):  
Kingsley Ebomah ◽  
Martins Adefisoye ◽  
Anthony Okoh
Keyword(s):  
Public Health ◽  
Escherichia Coli ◽  
South Africa ◽  
Neonatal Meningitis ◽  
Eastern Cape ◽  
Antibiotic Resistant ◽  
E Coli ◽  
Pathogenic Escherichia Coli ◽  
Susceptibility Profiles ◽  
The Impact

The prevalence of pathogenic microorganisms, as well as the proliferation of antimicrobial resistance, pose a significant threat to public health. However, the magnitude of the impact of aquatic environs concerning the advent and propagation of resistance genes remains vague. Escherichia coli (E. coli) are widespread and encompass a variety of strains, ranging from non-pathogenic to highly pathogenic. This study reports on the incidence and antibiotic susceptibility profiles of E. coli isolates recovered from the Nahoon beach and its canal waters in South Africa. A total of 73 out of 107 (68.2%) Polymerase chain reaction confirmed E. coli isolates were found to be affirmative for at least one virulence factor. These comprised of enteropathogenic E. coli 11 (10.3%), enteroinvasive E. coli 14 (13.1%), and neonatal meningitis E. coli 48 (44.9%). The phenotypic antibiogram profiles of the confirmed isolates revealed that all 73 (100%) were resistant to ampicillin, whereas 67 (91.8%) of the pathotypes were resistant to amikacin, gentamicin, and ceftazidime. About 61 (83.6%) and 51 (69.9%) were resistant to tetracycline and ciprofloxacin, respectively, and about 21.9% (16) demonstrated multiple instances of antibiotic resistance, with 100% exhibiting resistance to eight antibiotics. The conclusion from our findings is that the Nahoon beach and its canal waters are reservoirs of potentially virulent and antibiotic-resistant E. coli strains, which thus constitute a potent public health risk.

scholarly journals Effect of a lytic bacteriophage on rabbits experimentally infected with pathogenic Escherichia coli

2017 ◽  
Vol 25 (3) ◽  
pp. 273 ◽  
Author(s):  
J. Zhao ◽  
L. He ◽  
L. Pan ◽  
Y. Liu ◽  
H. Yao ◽  
...  
Keyword(s):  
Escherichia Coli ◽  
Burst Size ◽  
Lethal Dose ◽  
Resistant Bacteria ◽  
Lytic Bacteriophage ◽  
Antibiotic Resistant ◽  
E Coli ◽  
Pathogenic Escherichia Coli ◽  
One Step ◽  
The One

Pathogenic <em>Escherichia coli</em> (<em>E. coli</em>) is severely threatening the rabbit industry in China, and the concern over antibiotic-resistant bacteria has given rise to an urgent need for antibiotic alternatives. In this study, a member (ZRP1) of the <em>Myoviridae</em> family was isolated from rabbit faeces using a strain of rabbit atypical enteropathogenic <em>E. coli</em> (ZR1) as host. The one-step growth curve indicated that the latent period was around 25 to 30 min and the burst size was 144±31 plaque-forming unit/cell. The rate of phage-resistant mutation was 7×10<sup>–5</sup>±4×10<sup>–5</sup>. When the bacteriophage input at the multiplicity of infection (MOI) was 0.1, 1 or 10, the growth of host <em>E. coli</em> in broth was inhibited for 5 h. A single intravenous injection of ZRP1 at MOI 0.1, 1 or 10 significantly prolonged the survival time of rabbits which simultaneously received a lethal dose of ZR1.

scholarly journals Sub-lethal Concentrations of Phytochemicals (Carvacrol and Oregano) Select for Reduced Susceptibility Mutants of Escherichia coli O23:H52

2020 ◽  
Vol 69 (1) ◽  
pp. 121-125
Author(s):  
AFNAN A. AL-MNASER ◽  
MARTIN J. WOODWARD
Keyword(s):  
Escherichia Coli ◽  
Antibiotic Resistance ◽  
Coli Strain ◽  
Whole Genome ◽  
Avian Pathogenic Escherichia Coli ◽  
Antibiotic Resistant ◽  
E Coli ◽  
Pathogenic Escherichia Coli ◽  
Survival Mechanisms

In vitro studies aimed at studying the mechanism of action of carvacrol and oregano as natural anti-bacterial agents to control multiple antibiotic-resistant avian pathogenic Escherichia coli (APEC) strain O23:H52 isolated from chicken were performed. Derivatives with increased minimum inhibitory concentrations (MIC) to the phytochemicals were selected after growing Escherichia coli (E. coli) strain O23:H52 at sub-lethal concentrations of carvacrol and oregano for a period of 60 days. Whole-genome sequencing (WGS) of two derivatives revealed a missense mutation in cadC and marR: the genes responsible for survival mechanisms and antibiotic resistance by efflux, respectively.

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