scholarly journals Exogenous Polyamines Influence In Vitro Microbial Adhesion to Human Mucus according to the Age of Mucus Donor

2021 ◽  
Vol 9 (6) ◽  
pp. 1239
Author(s):  
Anastasia Mantziari ◽  
Enni Mannila ◽  
Maria Carmen Collado ◽  
Seppo Salminen ◽  
Carlos Gómez-Gallego
Keyword(s):  
Bacterial Adhesion ◽  
Cronobacter Sakazakii ◽  
Adhesive Properties ◽  
E Coli ◽  
Bifidobacterium Animalis ◽  
Adhesion Ability ◽  
Intestinal Mucus ◽  
Early Infant ◽  
Healthy Infants

Adhesion to intestinal mucus is the first step for microbiota colonization in early life. Polyamines are polycations with important physiological functions in both procaryotic and eucaryotic cells. However, their role in intestinal mucus adhesion is not known. The objective of the present study was to evaluate whether exogenous polyamines (putrescine, spermidine, spermine, and their combination) would alter the adhesive properties of Lacticaseibacillus rhamnosus GG (LGG), Bifidobacterium animalis subs. lactis Bb12, Cronobacter sakazakii, and Escherichia coli. Human intestinal mucus was isolated from healthy infants (0–6-month-old and 6–12-month-old) and healthy adults (25–52 years old). Spermidine significantly increased Bb12 adhesion (p < 0.05) in the mucus of infants (0–6 months) but reduced the adhesion of LGG in adult mucus (p < 0.05) with no significant effect in any of the infant groups. Spermine was more effective than polyamine combinations in reducing C. sakazakii (p < 0.05) adhesion in early infant mucus (0–6 months). The adhesion ability of E. coli remained unaffected by exogenous polyamines at any age in the concentrations tested. Our data suggest that polyamines may modulate the bacterial adhesion to mucus depending on the bacterial strain and depending at what age the mucus has been generated.

scholarly journals Species- and Age/Generation-Dependent Adherence of Bifidobacterium bifidum to Human Intestinal Mucus In Vitro

2021 ◽  
Vol 9 (3) ◽  
pp. 542
Author(s):  
Gaku Harata ◽  
Kazutoyo Yoda ◽  
Ruipeng Wang ◽  
Kenji Miyazawa ◽  
Masayuki Sato ◽  
...  
Keyword(s):  
Type Strain ◽  
Critical Role ◽  
Bifidobacterium Bifidum ◽  
Adhesion Properties ◽  
Intestinal Microbes ◽  
Bifidobacterium Animalis ◽  
Intestinal Mucus ◽  
Healthy Infants ◽  
Strong Adhesion

Adhesion to intestinal mucus is the first event in the process by which intestinal microbes colonize the intestine. It plays a critical role in the initiation of interactions between gut microbes and host animals. Despite the importance, the adhesion properties of probiotics are generally characterized using porcine mucin; adhesion to human mucus has been poorly characterized. In the present study, human intestinal mucus samples were isolated from 114 fecal samples collected from healthy infants and adults. In initial screening, four out of the 13 beneficial microbes tested, including the type strain of Bifidobacterium bifidum, B. bifidum TMC3115, Lacticaseibacillus rhamnosus GG, and Bifidobacterium animalis subsp. lactis Bb12, showed strong adhesion abilities to human mucus. The type strain of B. bifidum and TMC3115 adhered more strongly to neonatal and infant mucus than to adult mucus, while L. rhamnosus GG and B. lactis Bb12 adhered more strongly to adult mucus than to infant mucus. Similar results were obtained for ten additional strains of B. bifidum. In conclusion, age/generation-related differences were observed in the adhesion properties of B. bifidum and other strains. A deeper symbiotic relationship may exist between infants, particularly neonates, and B. bifidum based on its enhanced adhesion to neonatal intestinal mucus.

scholarly journals Screening the ability of natural feed ingredients to interfere with the adherence of enterotoxigenicEscherichia coli(ETEC) K88 to the porcine intestinal mucus

2013 ◽  
Vol 111 (4) ◽  
pp. 633-642 ◽  
Author(s):  
Gemma González-Ortiz ◽  
José Francisco Pérez ◽  
Rafael Gustavo Hermes ◽  
Francesc Molist ◽  
Rufino Jiménez-Díaz ◽  
...  
Keyword(s):  
Adhesion Test ◽  
Weaned Piglets ◽  
Adhesive Properties ◽  
Feed Ingredients ◽  
Recognition Process ◽  
Intestinal Mucus ◽  
Novel Approach ◽  
Carbohydrate Components ◽  
Water Extractable

The inhibition of the attachment of bacteria to the intestine by receptor analogues could be a novel approach to prevent enterotoxigenicEscherichia coli(ETEC) K88-induced diarrhoea in piglets. The objective of the present study was to screen the ability of different feed ingredients (FI) to bind to ETEC K88 (adhesion test, AT) and to block its attachment to the porcine intestinal mucus (blocking test, BT) usingin vitromicrotitration-based models. In the AT, wheat bran (WB), casein glycomacropeptide (CGMP) and exopolysaccharides exhibited the highest adhesion to ETEC K88 (P< 0·001). In the BT, WB, CGMP and locust bean (LB) reduced the number of ETEC K88 attached to the intestinal mucus (P< 0·001). For WB and LB, fractionation based on their carbohydrate components was subsequently carried out, and each fraction was evaluated individually. None of the WB fractions reduced the adhesion of ETEC K88 to the mucus as did the original extract, suggesting that a protein or glycoprotein could be involved in the recognition process. With regard to the LB fractions, the water-extractable material reduced the adhesion of ETEC K88 (P< 0·001) to the mucus similar to the original extract (P< 0·001), indicating, in this case, that galactomannans or phenolic compounds could be responsible for the recognition process. In conclusion, among the FI screened, the soluble extracts obtained from WB, LB and CGMP exhibited the highest anti-adhesive properties against ETEC K88 in the BT. These results suggest that they may be good candidates to be included in diets of weaned piglets for the prevention of ETEC K88-induced diarrhoea.

scholarly journals Justification of sorption composite usage on the basis of nanodisperse silica for sepsis prevention in complex treatment of purulent-inflammatory processes

2019 ◽  
Vol 41 part 3 (2) ◽  
pp. 45-50
Author(s):  
O. A. Viltsaniuk ◽  
P. V. Belyaev ◽  
T. P. Osolodchenko ◽  
O. O. Viltsaniuk
Keyword(s):  
Antimicrobial Activity ◽  
Antimicrobial Properties ◽  
Statistical Processing ◽  
Toxic Substances ◽  
Adhesive Properties ◽  
Adhesion Properties ◽  
E Coli ◽  
Adhesion Ability ◽  
Treatment And Prevention ◽  
Inflammatory Processes

Introduction. The problem of purulent-inflammatory processes treatment and the prevention of sepsis remains one of the most pressing problems of surgery.Purpose of the study. Is to estimate the effectiveness of sorption composition with antimicrobial properties on the basis of nanodisperse silica on the adhesive properties of microorganisms and to substantiate the expediency of its use for the purulent and inflammatory processes treatment and prevention of sepsis.Materials and methods.To determine the antimicrobial activity and the influence of the antiseptics of decasan, myramistin, ethonium, chlorhexidine and the sorption on the microorganisms adhesion properties composition was used the method of diffusion in agar of the museum strains of microorganisms S. Aureus ATCC 25923, E. Coli ATCC 25922, R. Vulgaris 4636, R. Aureginosa ATCC 27853, S. AIbicans 855/653, SI. Perfringens 28, Peptostreptococcus anaerobicus 22, Bacteroides fragilis 13/83, and clinical strains of S. Aureus, R. Aeuroginosa, E. Coli. Adhesive properties of microorganisms were studied according to the Brillis method et al. (1986) with the subsequent statistical processing of the data.Results. Studies have shown that antiseptics have high antimicrobial activity and reduce the adhesion ability of microorganisms. But the use of only antiseptics or only antibiotics for the purification of purulent foci can lead to toxins absorption and tissue decay products from purulent foci. That processes could be the trigger mechanism for sepsis development. The sorption composition is able to reduce the adhesive activity of bacteria, allows to absorb toxic substances from pathological foci, what preventing their resorption and thereby prevent the development of sepsis.Conclusions. The composition made from sorbents and antiseptics is not inferior to antimicrobial activity to antiseptics, which can reduce the virulence of bacteria, by reducing their adhesive activity, and absorb toxins of microorganisms and tissue decomposition products on their surface, thus prevent their absorption, which will prevent the purulent-inflammatory process progression and sepsis development.Keywords: sorption composition, nanodisperse silica, antiseptics, adhesive properties of microorganisms, prevention of sepsis.

scholarly journals Screening of Escherichia coli Species Biodiversity Reveals New Biofilm-Associated Antiadhesion Polysaccharides

mBio ◽  
2011 ◽  
Vol 2 (3) ◽  
Author(s):  
Olaya Rendueles ◽  
Laetitia Travier ◽  
Patricia Latour-Lambert ◽  
Thierry Fontaine ◽  
Julie Magnus ◽  
...  
Keyword(s):  
Escherichia Coli ◽  
Staphylococcus Aureus ◽  
Bacterial Adhesion ◽  
Bioactive Molecules ◽  
Bacterial Biofilms ◽  
Gram Positive ◽  
Content Type ◽  
E Coli ◽  
Species Biodiversity

ABSTRACTBacterial biofilms often form multispecies communities in which complex but ill-understood competition and cooperation interactions occur. In light of the profound physiological modifications associated with this lifestyle, we hypothesized that the biofilm environment might represent an untapped source of natural bioactive molecules interfering with bacterial adhesion or biofilm formation. We produced cell-free solutions extracted fromin vitromature biofilms formed by 122 naturalEscherichia coliisolates, and we screened these biofilm extracts for antiadhesion molecules active on a panel of Gram-positive and Gram-negative bacteria. Using this approach, we showed that 20% of the tested biofilm extracts contained molecules that antagonize bacterial growth or adhesion. We characterized a compound, produced by a commensal animalE. colistrain, for which activity is detected only in biofilm extract. Biochemical and genetic analyses showed that this compound corresponds to a new type of released high-molecular-weight polysaccharide whose biofilm-associated production is regulated by the RfaH protein. We demonstrated that the antiadhesion activity of this polysaccharide was restricted to Gram-positive bacteria and that its production reduced susceptibility to invasion and provided rapid exclusion ofStaphylococcus aureusfrom mixedE. coliandS. aureusbiofilms. Our results therefore demonstrate that biofilms contain molecules that contribute to the dynamics of mixed bacterial communities and that are not or only poorly detected in unconcentrated planktonic supernatants. Systematic identification of these compounds could lead to strategies that limit pathogen surface colonization and reduce the burden associated with the development of bacterial biofilms on medical devices.IMPORTANCEWe sought to demonstrate that bacterial biofilms are reservoirs for unknown molecules that antagonize bacterial adhesion. The use of natural strains representative ofEscherichia colispecies biodiversity showed that nonbiocidal antiadhesion polysaccharides are frequently found in mature biofilm extracts (bacterium-free suspensions which contain soluble molecules produced within the biofilm). Release of an antiadhesion polysaccharide confers a competitive advantage upon the producing strain against clinically relevant pathogens such asStaphylococcus aureus. Hence, exploring the biofilm environment provides a better understanding of bacterial interactions within complex communities and could lead to improved control of pathogen colonization.

Bioactive and Antibacterial Ag-AgCl-TiO2 Coating on Titanium Implants

2016 ◽  
Vol 852 ◽  
pp. 1213-1219 ◽  
Author(s):  
Tian Tian ◽  
Liu Hui ◽  
Gu Ming Jun ◽  
Jin Ying
Keyword(s):  
Bacterial Adhesion ◽  
Antibacterial Property ◽  
Titanium Implants ◽  
Material Surface ◽  
X Ray Diffraction ◽  
E Coli ◽  
Implant Material ◽  
Titanium Substrates ◽  
Soaking Test

In this work, Ag-AgCl-TiO2 coating was fabricated on titanium substrates to obtain an implant material having excellent antibacterial property and bioactivity. The coating was investigated by scanning electron microscopy and X-ray diffraction. The bioactivity of coatings was examined by simulated body fluid soaking test. To verify the susceptibility of implant material surface to bacterial adhesion, S. aureus (Sau), E. coli (Eco), K. pneumoniae (Kpn), P. Aeruginosa (Pae), four types of major pathogen were chosen for in vitro antibacterial analyses. The results showed that Ag-AgCl-TiO2 coating had excellent antibacterial property and bioactivity.

scholarly journals Grapevine extracts and their effect on selected gut-associated microbiota: In vitro study

2020 ◽  
Vol 38 (No. 3) ◽  
pp. 137-143
Author(s):  
Michaela Rollová ◽  
Lucia Gharwalova ◽  
Aleš Krmela ◽  
Věra Schulzová ◽  
Jana Hajšlová ◽  
...  
Keyword(s):  
Inhibitory Concentration ◽  
In Vitro Study ◽  
Probiotic Strain ◽  
Individual Species ◽  
Biofilm Growth ◽  
E Coli ◽  
Bifidobacterium Animalis ◽  
The Individual ◽  
Opportunistic Pathogenic

The biological activity of polyphenol substances contained in food supplements prepared from Vitis vinifera can affect the microorganisms present in the digestive tract in terms of their representation and activity of the individual species. This study deals with resveratrol and two polyphenol-rich extracts (extract from V. vinifera canes and the commercial product Regrapex-R-forte) and their effect on selected gut microbiota (Bifidobacterium animalis subsp. lactis Bb-12, Lactobacillus acidophilus LA-5, Lactobacillus casei Lafti L-26, Citrobacter freundii DBM 3127, Escherichia coli DBM 3125). The effect of the studied agents on planktonic and biofilm growth of the microorganisms was determined as minimum inhibitory concentration (MIC80) and minimum biofilm inhibitory concentration (MBIC80), respectively. The extracts induced metabolic activity as well as total biofilm biomass production in probiotic strain L. acidophilus LA-5 while successfully inhibiting the growth of opportunistic pathogenic microorganisms C. freundii DBM 3127 and E. coli DBM 3125.

Adhesion of Dairy Propionibacteria to Intestinal Epithelial Tissue In Vitro and In Vivo

2002 ◽  
Vol 65 (3) ◽  
pp. 534-539 ◽  
Author(s):  
GABRIELA ZÁRATE ◽  
VILMA I. MORATA de AMBROSINI ◽  
ADRIANA PEREZ CHAIA ◽  
SILVIA N. GONZÁLEZ
Keyword(s):  
Surface Characteristics ◽  
Epithelial Tissue ◽  
Intestinal Epithelial ◽  
Adhesive Properties ◽  
Bacterial Surface ◽  
Beneficial Effects ◽  
Adhesion Ability ◽  
Dairy Propionibacteria

Adhesion to the intestinal mucosa is a desirable property for probiotic microorganisms and has been related to many of their health benefits. In the present study, 24 dairy Propionibacterium strains were assessed with regard to their hydrophobic characteristics and their autoaggregation and hemagglutination abilities, since these traits have been shown to be indicative of adherence in other microorganisms. Six strains were further tested for their capacity to adhere to ileal epithelial cells in vitro and in vivo. The results of the study showed that propionibacteria were highly hydrophilic, and hemagglutination and autoaggregation were properties not commonly found among these microorganisms. No relationship was found between surface characteristics and adhesion ability, since hemagglutinating, autoaggregating, and nonautoaggregating bacteria were able to adhere to intestinal cells both in vitro and in vivo. Microscopic examination revealed that autoaggregating cells adhered in clusters, with adhesion being mediated by only a few bacteria, whereas the hemagglutinating and nonautoaggregating strains adhered individually or in small groups making contact with each epithelial cell with the entire bacterial surface. The in vitro assessment of adhesion was a good indication of the in vivo association of propionibacteria with the intestinal epithelium. Therefore, the in vitro method presented here should be valuable in screening routinely adhesive properties of propionibacteria for probiotic purposes. The adhesion ability of dairy propionibacteria would prolong their maintenance in the gut and increase the duration of their provision of beneficial effects in the host, supporting the potential of Propionibacterium in the development of new probiotic products.

scholarly journals A Novel Strategy for Creating an Antibacterial Surface Using a Highly Efficient Electrospray-Based Method for Silica Deposition

2022 ◽  
Vol 23 (1) ◽  
pp. 513
Author(s):  
Odelia Levana ◽  
Soonkook Hong ◽  
Se Hyun Kim ◽  
Ji Hoon Jeong ◽  
Sung Sik Hur ◽  
...  
Keyword(s):  
Bacterial Adhesion ◽  
Cost Effective ◽  
Dermal Fibroblasts ◽  
Root Mean Square Roughness ◽  
Adhesive Properties ◽  
Average Roughness ◽  
Water Contact ◽  
Biomedical Implant ◽  
E Coli ◽  
Antibacterial Surface

Adhesion of bacteria on biomedical implant surfaces is a prerequisite for biofilm formation, which may increase the chances of infection and chronic inflammation. In this study, we employed a novel electrospray-based technique to develop an antibacterial surface by efficiently depositing silica homogeneously onto polyethylene terephthalate (PET) film to achieve hydrophobic and anti-adhesive properties. We evaluated its potential application in inhibiting bacterial adhesion using both Gram-negative Escherichia coli (E. coli) and Gram-positive Staphylococcus aureus (S. aureus) bacteria. These silica-deposited PET surfaces could provide hydrophobic surfaces with a water contact angle greater than 120° as well as increased surface roughness (root mean square roughness value of 82.50 ± 16.22 nm and average roughness value of 65.15 ± 15.26 nm) that could significantly reduce bacterial adhesion by approximately 66.30% and 64.09% for E. coli and S. aureus, respectively, compared with those on plain PET surfaces. Furthermore, we observed that silica-deposited PET surfaces showed no detrimental effects on cell viability in human dermal fibroblasts, as confirmed by MTT (3-(4,5-dimethylthiazol-2-yl)-2,5 diphenyl tetrazolium bromide and live/dead assays. Taken together, such approaches that are easy to synthesize, cost effective, and efficient, and could provide innovative strategies for preventing bacterial adhesion on biomedical implant surfaces in the clinical setting.

scholarly journals A SimpleIn VitroGut Model for Studying the Interaction betweenEscherichia coliand the Intestinal Commensal Microbiota in Cecal Mucus

2018 ◽  
Vol 84 (24) ◽  
Author(s):  
Matthew E. Mokszycki ◽  
Mary Leatham-Jensen ◽  
Jon L. Steffensen ◽  
Ying Zhang ◽  
Karen A. Krogfelt ◽  
...  
Keyword(s):  
Escherichia Coli ◽  
Gene Profiling ◽  
Rrna Gene ◽  
Mucus Layer ◽  
Content Type ◽  
E Coli ◽  
Commensal Microbiota ◽  
Intestinal Mucus

ABSTRACTA novelin vitrogut model was developed to better understand the interactions betweenEscherichia coliand the mouse cecal mucus commensal microbiota. The gut model is simple and inexpensive while providing an environment that largely replicates the nonadherent mucus layer of the mouse cecum. 16S rRNA gene profiling of the cecal microbial communities of streptomycin-treated mice colonized withE. coliMG1655 orE. coliNissle 1917 and the gut model confirmed that the gut model properly reflected the community structure of the mouse intestine. Furthermore, the results from thein vitrogut model mimic the results of publishedin vivocompetitive colonization experiments. The gut model is initiated by the colonization of streptomycin-treated mice, and then the community is serially transferred in microcentrifuge tubes in an anaerobic environment generated in anaerobe jars. The nutritional makeup of the cecum is simulated in the gut model by using a medium consisting of porcine mucin, mouse cecal mucus, HEPES-Hanks buffer (pH 7.2), Cleland’s reagent, and agarose. Agarose was found to be essential for maintaining the stability of the microbial community in the gut model. The outcome of competitions betweenE. colistrains in thein vitrogut model is readily explained by the “restaurant hypothesis” of intestinal colonization. This simple model system potentially can be used to more fully understand how different members of the microbiota interact physically and metabolically during the colonization of the intestinal mucus layer.IMPORTANCEBoth commensal and pathogenic strains ofEscherichia coliappear to colonize the mammalian intestine by interacting physically and metabolically with other members of the microbiota in the mucus layer that overlays the cecal and colonic epithelium. However, the use of animal models and the complexity of the mammalian gut make it difficult to isolate experimental variables that might dictate the interactions betweenE. coliand other members of the microbiota, such as those that are critical for successful colonization. Here, we describe a simple and relatively inexpensivein vitrogut model that largely mimicsin vivoconditions and therefore can facilitate the manipulation of experimental variables for studying the interactions ofE. coliwith the intestinal microbiota.

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