scholarly journals Microbial Adhesion and Biofilm Formation on Bioactive Surfaces of Ti-35Nb-7Zr-5Ta Alloy Created by Anodization

2021 ◽  
Vol 9 (10) ◽  
pp. 2154
Author(s):  
Laiza Maria Grassi Fais ◽  
Luana de Sales Leite ◽  
Bárbara Araújo dos Reis ◽  
Ana Lúcia Roselino Ribeiro ◽  
Luis Geraldo Vaz ◽  
...  
Keyword(s):  
Biofilm Formation ◽  
Surface Modifications ◽  
Microbial Colonization ◽  
Periodontal Pathogen ◽  
Polar Component ◽  
Silver Deposition ◽  
Microbial Adhesion ◽  
Actinomyces Naeslundii ◽  
Modified Surfaces ◽  
Before And After

This study evaluated the microbial colonization (adhesion and biofilm) on modified surfaces of a titanium alloy, Ti-35Nb-7Zr-5Ta, anodized with Ca and P or F ions, with and without silver deposition. The chemical composition, surface topography, roughness (Ra), and surface free energy were evaluated before and after the surface modifications (anodizing). Adhesion and biofilm formation on saliva-coated discs by primary colonizing species (Streptococcus sanguinis, Streptococcus gordonii, Actinomyces naeslundii) and a periodontal pathogen (Porphyromonasgingivalis) were assessed. The surfaces of titanium alloys were modified after anodizing with volcano-shaped micropores with Ca and P or nanosized with F, both with further silver deposition. There was an increase in the Ra values after micropores formation; CaP surfaces became more hydrophilic than other surfaces, showing the highest polar component. For adhesion, no difference was detected for S. gordonii on all surfaces, and some differences were observed for the other three species. No differences were found for biofilm formation per species on all surfaces. However, S. gordonii biofilm counts on distinct surfaces were lower than S. sanguinis, A. naeslundii, and P. gingivalis on some surfaces. Therefore, anodized Ti-35Nb-7Zr-5Ta affected microbial adhesion and subsequent biofilm, but silver deposition did not hinder the colonization of these microorganisms.

scholarly journals The Impact of Dental Implant Surface Modifications on Osseointegration and Biofilm Formation

2021 ◽  
Vol 10 (8) ◽  
pp. 1641
Author(s):  
Stefanie Kligman ◽  
Zhi Ren ◽  
Chun-Hsi Chung ◽  
Michael Angelo Perillo ◽  
Yu-Cheng Chang ◽  
...  
Keyword(s):  
Dental Implant ◽  
Biofilm Formation ◽  
Bacterial Colonization ◽  
Surface Modifications ◽  
Implant Surface ◽  
Oral Rehabilitation ◽  
Surface Design ◽  
Polyether Ether Ketone ◽  
Dental Implant Surface ◽  
The Impact

Implant surface design has evolved to meet oral rehabilitation challenges in both healthy and compromised bone. For example, to conquer the most common dental implant-related complications, peri-implantitis, and subsequent implant loss, implant surfaces have been modified to introduce desired properties to a dental implant and thus increase the implant success rate and expand their indications. Until now, a diversity of implant surface modifications, including different physical, chemical, and biological techniques, have been applied to a broad range of materials, such as titanium, zirconia, and polyether ether ketone, to achieve these goals. Ideal modifications enhance the interaction between the implant’s surface and its surrounding bone which will facilitate osseointegration while minimizing the bacterial colonization to reduce the risk of biofilm formation. This review article aims to comprehensively discuss currently available implant surface modifications commonly used in implantology in terms of their impact on osseointegration and biofilm formation, which is critical for clinicians to choose the most suitable materials to improve the success and survival of implantation.

scholarly journals Biofilm-FormingStaphylococcus epidermidisExpressing Vancomycin Resistance Early after Adhesion to a Metal Surface

2015 ◽  
Vol 2015 ◽  
pp. 1-8 ◽  
Author(s):  
Toshiyuki Sakimura ◽  
Shiro Kajiyama ◽  
Shinji Adachi ◽  
Ko Chiba ◽  
Akihiko Yonekura ◽  
...  
Keyword(s):  
Metal Surface ◽  
Staphylococcus Epidermidis ◽  
Biofilm Formation ◽  
Bacterial Count ◽  
Fluorescence Staining ◽  
Biofilm Growth ◽  
Before And After ◽  
Viable Bacteria ◽  
Coverage Rates ◽  
Resistance Expression

We investigated biofilm formation and time of vancomycin (VCM) resistance expression after adhesion to a metal surface inStaphylococcus epidermidis. Biofilm-formingStaphylococcus epidermidiswith a VCM MIC of 1 μg/mL was used. The bacteria were made to adhere to a stainless steel washer and treated with VCM at different times and concentrations. VCM was administered 0, 2, 4, and 8 hours after adhesion. The amount of biofilm formed was evaluated based on the biofilm coverage rates (BCRs) before and after VCM administration, bacterial viability in biofilm was visually observed using the fluorescence staining method, and the viable bacterial count in biofilm was measured. The VCM concentration required to decrease BCR significantly compared with that of VCM-untreated bacteria was 4 μg/mL, even in the 0 hr group. In the 4 and 8 hr groups, VCM could not inhibit biofilm growth even at 1,024 μg/mL. In the 8 hr group, viable bacteria remained in biofilm at a count of 104CFU even at a high VCM concentration (1,024 μg/mL). It was suggested that biofilm-formingStaphylococcus epidermidisexpresses resistance to VCM early after adhesion to a metal surface. Resistance increased over time after adhesion as the biofilm formed, and strong resistance was expressed 4–8 hours after adhesion.

Surface Modification of Cellulose Membrane by Air Plasma Treatment

2013 ◽  
Vol 770 ◽  
pp. 112-115
Author(s):  
Nawal Binhayeeniyi ◽  
Adinan Jehsu ◽  
Mancharee Sukpet ◽  
Safitree Nawae
Keyword(s):  
Surface Modification ◽  
Plasma Treatment ◽  
Surface Modifications ◽  
Contact Angles ◽  
Cellulose Membrane ◽  
Air Plasma ◽  
Before And After ◽  
Cellulose Membranes ◽  
Hydrophilic Membranes ◽  
Air Plasma Treatment

Low-temperature air plasma was used to treat the cellulose membranes by varying the period of time from 10 to 30 minutes. The surfaces of membranes were changed from hydrophobic to hydrophilic membranes. The contact angles of treated membranes were increased when increasing time to treat. The surface modifications of membrane before and after treated were characterized by SEM. It is shown that air plasma treatment is used to improve the roughness. The dielectric property was also studied.

scholarly journals Transient and Sustained Bacterial Adaptation following Repeated Sublethal Exposure to Microbicides and a Novel Human Antimicrobial Peptide

2014 ◽  
Vol 58 (10) ◽  
pp. 5809-5817 ◽  
Author(s):  
Sarah Forbes ◽  
Curtis B. Dobson ◽  
Gavin J. Humphreys ◽  
Andrew J. McBain
Keyword(s):  
Antimicrobial Peptide ◽  
Biofilm Formation ◽  
Bacterial Isolates ◽  
Exposure System ◽  
Polyhexamethylene Biguanide ◽  
Prevention And Treatment ◽  
Before And After ◽  
The Stability ◽  
Induced Changes ◽  
Laboratory Exposure

ABSTRACTMicrobicides (biocides) play an important role in the prevention and treatment of infections. While there is currently little evidence for in-use treatment failures attributable to acquired reductions in microbicide susceptibility, the susceptibility of some bacteria can be reduced by sublethal laboratory exposure to certain agents. In this investigation, a range of environmental bacterial isolates (11 genera, 18 species) were repeatedly exposed to four microbicides (cetrimide, chlorhexidine, polyhexamethylene biguanide [PHMB], and triclosan) and a cationic apolipoprotein E-derived antimicrobial peptide (apoEdpL-W) using a previously validated exposure system. Susceptibilities (MICs and minimum bactericidal concentrations [MBCs]) were determined before and after 10 passages (P10) in the presence of an antimicrobial and then after a further 10 passages without an antimicrobial to determine the stability of any adaptations. Bacteria exhibiting >4-fold increases in MBCs were further examined for alterations in biofilm-forming ability. Following microbicide exposure, ≥4-fold decreases in susceptibility (MIC or MBC) occurred for cetrimide (5/18 bacteria), apoEdpL-W (7/18), chlorhexidine (8/18), PHMB (8/18), and triclosan (11/18). Of the 34 ≥4-fold increases in the MICs, 15 were fully reversible, 13 were partially reversible, and 6 were nonreversible. Of the 26 ≥4-fold increases in the MBCs, 7 were fully reversible, 14 were partially reversible, and 5 were nonreversible. Significant decreases in biofilm formation in P10 strains occurred for apoEdpL-W (1/18 bacteria), chlorhexidine (1/18), and triclosan (2/18), while significant increases occurred for apoEdpL-W (1/18), triclosan (1/18), and chlorhexidine (2/18). These data indicate that the stability of induced changes in microbicide susceptibility varies but may be sustained for some combinations of a bacterium and a microbicide.

scholarly journals Highly variable effect of sonication to dislodge biofilm-embedded Staphylococcus epidermidis directly quantified by epifluorescence microscopy: an in vitro model study

2020 ◽  
Vol 15 (1) ◽  
Author(s):  
Erik T. Sandbakken ◽  
Eivind Witsø ◽  
Bjørnar Sporsheim ◽  
Kjartan W. Egeberg ◽  
Olav A. Foss ◽  
...  
Keyword(s):  
Staphylococcus Epidermidis ◽  
Biofilm Formation ◽  
In Vitro Model ◽  
Laser Scanning Microscopy ◽  
Epifluorescence Microscopy ◽  
Variable Effect ◽  
Prosthetic Joint Infections ◽  
Before And After ◽  
Vitro Model

Abstract Background In cases of prosthetic joint infections, culture of sonication fluid can supplement culture of harvested tissue samples for correct microbial diagnosis. However, discrepant results regarding the increased sensitivity of sonication have been reported in several studies. To what degree bacteria embedded in biofilm are dislodged during the sonication process has to our knowledge not been fully elucidated. In the present in vitro study, we have evaluated the effect of sonication as a method to dislodge biofilm by quantitative microscopy. Methods We used a standard biofilm method to cover small steel plates with biofilm forming Staphylococcus epidermidis ATCC 35984 and carried out the sonication procedure according to clinical practice. By comparing area covered with biofilm before and after sonication with epifluorescence microscopy, the effect of sonication on biofilm removal was quantified. Two series of experiments were made, one with 24-h biofilm formation and another with 72-h biofilm formation. Confocal laser scanning microscopy (CLSM) and scanning electron microscopy (SEM) were used to confirm whether bacteria were present after sonication. In addition, quantitative bacteriology of sonication fluid was performed. Results Epifluorescence microscopy enabled visualization of biofilm before and after sonication. CLSM and SEM confirmed coccoid cells on the surface after sonication. Biofilm was dislodged in a highly variable manner. Conclusion There is an unexpected high variation seen in the ability of sonication to dislodge biofilm-embedded S. epidermidis in this in vitro model.

scholarly journals Comparison of microbial adhesion and biofilm formation on orthodontic wax materials; an in vitro study

2020 ◽  
Vol 15 (4) ◽  
pp. 493-499
Author(s):  
Aylin Pasaoglu Bozkurt ◽  
Özge Ünlü ◽  
Mehmet Demirci
Keyword(s):  
Biofilm Formation ◽  
In Vitro Study ◽  
Vitro Study ◽  
Microbial Adhesion

scholarly journals Polymicrobial biofilm formation byCandida albicans, Actinomyces naeslundii, andStreptococcus mutansisCandida albicansstrain and medium dependent

2016 ◽  
Vol 54 (8) ◽  
pp. 856-864 ◽  
Author(s):  
Mohd Hafiz Arzmi ◽  
Ali D. Alnuaimi ◽  
Stuart Dashper ◽  
Nicola Cirillo ◽  
Eric C. Reynolds ◽  
...  
Keyword(s):  
Biofilm Formation ◽  
Actinomyces Naeslundii

scholarly journals Predictability of Microbial Adhesion to Dental Materials by Roughness Parameters

Coatings ◽  
2019 ◽  
Vol 9 (7) ◽  
pp. 456 ◽  
Author(s):  
Andrea Schubert ◽  
Torsten Wassmann ◽  
Mareike Holtappels ◽  
Oliver Kurbad ◽  
Sebastian Krohn ◽  
...  
Keyword(s):  
Surface Roughness ◽  
Titanium Alloy ◽  
Biofilm Formation ◽  
Dental Materials ◽  
Roughness Parameter ◽  
In Vitro Study ◽  
Mean Deviation ◽  
Roughness Parameters ◽  
Microbial Adhesion

Microbial adhesion to intraoral biomaterials is associated with surface roughness. For the prevention of oral pathologies, smooth surfaces with little biofilm formation are required. Ideally, appropriate roughness parameters make microbial adhesion predictable. Although a multitude of parameters are available, surface roughness is commonly described by the arithmetical mean roughness value (Ra). The present study investigates whether Ra is the most appropriate roughness parameter in terms of prediction for microbial adhesion to dental biomaterials. After four surface roughness modifications using standardized polishing protocols, zirconia, polymethylmethacrylate, polyetheretherketone, and titanium alloy specimens were characterized by Ra as well as 17 other parameters using confocal microscopy. Specimens of the tested materials were colonized by C. albicans or S. sanguinis for 2 h; the adhesion was measured via luminescence assays and correlated with the roughness parameters. The adhesion of C. albicans showed a tendency to increase with increasing the surface roughness—the adhesion of S. sanguinis showed no such tendency. Although Sa, that is, the arithmetical mean deviation of surface roughness, and Rdc, that is, the profile section height between two material ratios, showed higher correlations with the microbial adhesion than Ra, these differences were not significant. Within the limitations of this in-vitro study, we conclude that Ra is a sufficient roughness parameter in terms of prediction for initial microbial adhesion to dental biomaterials with polished surfaces.

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