scholarly journals Mineral Fibres and Asbestos Bodies in Human Lung Tissue: A Case Study

Minerals ◽  
2019 ◽  
Vol 9 (10) ◽  
pp. 618 ◽  
Author(s):  
Di Giuseppe ◽  
Zoboli ◽  
Vigliaturo ◽  
Gieré ◽  
Bonasoni ◽  
...  
Keyword(s):  
Lung Tissue ◽  
Animal Studies ◽  
Amorphous Layer ◽  
Microscopy Study ◽  
Electron Microscopy Study ◽  
The Body ◽  
Degree Of Crystallinity ◽  
Human Lung Tissue ◽  
Improved Model

One of the open questions regarding the asbestos problem is the fate of the mineral fibres in the body once inhaled and deposited in the deep respiratory system. In this context, the present paper reports the results of an electron microscopy study of both mineral fibres and asbestos bodies found in the lung tissue of a patient who died of malignant mesothelioma due to past occupational exposure. In concert with previous in vivo animal studies, our data provide evidence that amphibole asbestos fibres are durable in the lungs, whereas chrysotile fibres are transformed into a silica‐rich product, which can be easily cleared. Amphibole fibres recovered from samples of tissue of the deceased display a high degree of crystallinity but also show a very thin amorphous layer on their surface; 31% of the fibres are coated with asbestos bodies consisting of a mixture of ferroproteins (mainly ferritin). Here, we propose an improved model for the coating process. Formation of a coating on the fibres is a defence mechanism against fibres that are longer than 10 µm and thinner than 0.5 µm, which macrophages cannot engulf. The mature asbestos bodies show signs of degradation, and the iron stored in ferritin may be released and potentially increase oxidative stress in the lung tissue.

Cytological changes induced by platinum-thymine in sarcoma-180 cells: Electron microscopy study

1990 ◽  
Vol 48 (3) ◽  
pp. 290-291
Author(s):  
Gustav Ofosu
Keyword(s):  
Mammalian Cells ◽  
Antitumor Agent ◽  
Microscopy Study ◽  
Electron Microscopy Study ◽  
Limiting Factor ◽  
Sarcoma 180 ◽  
Electron Microscopic ◽  
Cytological Changes

Platinum-thymine has been found to be a potent antitumor agent, which is quite soluble in water, and lack nephrotoxicity as the dose-limiting factor. The drug has been shown to interact with DNA and inhibits DNA, RNA and protein synthesis in mammalian cells in vitro. This investigation was undertaken to elucidate the cytotoxic effects of piatinum-thymine on sarcoma-180 cells in vitro ultrastructurally, Sarcoma-180 tumor bearing mice were treated with intraperitoneal injection of platinum-thymine 40mg/kg. A concentration of 60μg/ml dose of platinum-thymine was used in in vitro experiments. Treatments were at varying time intervals of 3, 7 and 21 days for in vivo experiments, and 30, 60 and 120 min., 6, 12, and 24th in vitro. Controls were not treated with platinum-thymine.Electron microscopic analyses of the treated cells in vivo and in vitro showed drastic cytotoxic effect.

scholarly journals Ultrastructural alterations of intracellular stages and effects on blood forms of Trypanosoma cruzi induced in vivo by 2-amino-5-(1-methyil-5-nitro-2-imidazolyl)-1,3,4 - Thiadiazole

1984 ◽  
Vol 17 (2) ◽  
pp. 89-93 ◽  
Author(s):  
Thaisa de Almeida Maria ◽  
Leny de Sousa Filardi ◽  
Zigman Brener
Keyword(s):  
Electron Microscopy ◽  
Single Dose ◽  
Trypanosoma Cruzi ◽  
Drug Administration ◽  
Active Drug ◽  
Developmental Stages ◽  
Microscopy Study ◽  
Electron Microscopy Study ◽  
Ultrastructural Alterations

An electron microscopy study shows that the administration of a single dose (500 mg/kg, p.o.) of 2-amino-5-(1-methyl-5-nitro-2-imidazolyl)-1, 3, 4-thiadiazole induces in mice infected with Trypanosoma cruzi results in degenerative lesions of the intracellular stages. Ultrastructural alterations are detected as early as 6 hours after the drug administration and destruction of the parasites occurs within 18 - 36 hours. Trypomastigotes are cleared from the bloodstream 4 to 6 hours after treatment. The combined effect on both developmental stages is apparently responsible for the in vivo ejfects of this drug which is the most active drug ever tested in our laboratory in experimental Chagas' disease.

The effect of benzalkonium chloride on rabbit nasal mucosa in vivo: an electron microscopy study

2002 ◽  
Vol 259 (7) ◽  
pp. 362-364 ◽  
Author(s):  
Sebahattin Cüreoğlu ◽  
Murat Akkuş ◽  
Üstün Osma ◽  
Mehmet Yaldiz ◽  
Faruk Oktay ◽  
...  
Keyword(s):  
Electron Microscopy ◽  
Nasal Mucosa ◽  
Benzalkonium Chloride ◽  
Microscopy Study ◽  
Electron Microscopy Study

Challenges on the effect of cell phone radiation on mammalian embryos and fetuses: a review of the literature

Zygote ◽  
2021 ◽  
pp. 1-7
Author(s):  
Maryam Mahaldashtian ◽  
Mohammad Ali Khalili ◽  
Fatemeh Anbari ◽  
Mohammad Seify ◽  
Manuel Belli
Keyword(s):  
Embryo Development ◽  
Animal Studies ◽  
Cell Phones ◽  
Cellular Phone ◽  
The Body ◽  
Human Embryos ◽  
Success Rates ◽  
Wide Range

Summary Cell phones operate with a wide range of frequency bands and emit radiofrequency-electromagnetic radiation (RF-EMR). Concern on the possible health hazards of RF-EMR has been growing in many countries because these RF-EMR pulses may be absorbed into the body cells, directly affecting them. There are some in vitro and in vivo animal studies related to the consequences of RF-EMR exposure from cell phones on embryo development and offspring. In addition, some studies have revealed that RF-EMR from cellular phone may lead to decrease in the rates of fertilization and embryo development, as well as the risk of the developmental anomalies, other studies have reported that it does not interfere with in vitro fertilization or intracytoplasmic sperm injection success rates, or the chromosomal aberration rate. Of course, it is unethical to study the effect of waves generated from cell phones on the forming human embryos. Conversely, other mammals have many similarities to humans in terms of anatomy, physiology and genetics. Therefore, in this review we focused on the existing literature evaluating the potential effects of RF-EMR on mammalian embryonic and fetal development.

scholarly journals Distribution of inhaled fluticasone propionate between human lung tissue and serum in vivo

1997 ◽  
Vol 10 (7) ◽  
pp. 1496-1499 ◽  
Author(s):  
N. Esmailpour ◽  
P. Högger ◽  
K.F. Rabe ◽  
U. Heitmann ◽  
M. Nakashima ◽  
...  
Keyword(s):  
Fluticasone Propionate ◽  
Lung Tissue ◽  
Human Lung ◽  
Human Lung Tissue

scholarly journals Gap junction internalization and processing in vivo: a 3D immuno-electron microscopy study

2020 ◽  
pp. jcs.252726
Author(s):  
Rachael P. Norris ◽  
Mark Terasaki
Keyword(s):  
Gap Junctions ◽  
Gap Junction ◽  
Connexin 43 ◽  
New Technology ◽  
Cell Communication ◽  
Membrane Vesicles ◽  
Microscopy Study ◽  
Electron Microscopy Study ◽  
Annular Gap

Gap junctions have well-established roles in cell-cell communication by way of forming permeable intercellular channels. Less is understood about their internalization, which forms double membrane vesicles containing cytosol and membranes from another cell, called connexosomes or annular gap junctions. Here, we systematically investigated the fate of connexosomes in intact ovarian follicles. High pressure frozen, serial sectioned tissue was immunogold labeled for Connexin 43. Within a volume corresponding to ∼35 cells, every labeled structure was categorized and its surface area was measured. Measurements support the concept that multiple connexosomes form from larger invaginated gap junctions. Subsequently, the inner and outer membranes separate, Cx43 immunogenicity is lost from the outer membrane, and the inner membrane appears to undergo fission. One pathway for processing involves lysosomes, based on localization of Cathespin B to some processed connexosomes. In summary, this study demonstrates new technology for high-resolution analyses of gap junction processing.

High-Resolution Transmission Electron Microscopy Study of Metallic Spin-Glass/Amorphous Silicon Multilayers

1995 ◽  
Vol 382 ◽  
Author(s):  
David A. Howell ◽  
Martin A. Crimp ◽  
Lilian M. Hoines ◽  
J. Bass
Keyword(s):  
Electron Microscopy ◽  
Transmission Electron Microscopy ◽  
High Resolution ◽  
Spin Glass ◽  
Amorphous Silicon ◽  
Microscopy Study ◽  
Electron Microscopy Study ◽  
Degree Of Crystallinity ◽  
Multilayer Systems ◽  
Transmission Electron

ABSTRACTHigh-resolution transmission electron microscopy has been used to investigate the structure and growth behavior of three separate multilayer systems composed of spin-glass alloys(AuFe.03,CuMn.15, and AgMn.09) alternating with amorphous silicon. Each of the three systems was fabricated with two different sample configurations. The first consisted of bilayers with 3 nm spinglass alloy and 7 nm amorphous siliconlayers. The second consisted of 7 nm spin-glass alloy and 7 nm amorphous silicon layers. HRTEM images of ion-milled cross-sectioned samples revealed variations in the degree of crystallinity of the spin-glass material. Variations in the amount and symmetry of interlayer formation were also observed. Systematic studies of such variations should help to explain differences in their measured spin-glass properties.

Transmission Electron Microscopy Study on the Surface Properties of CNTs and Fullerites Exposed to CF4 Plasma

2007 ◽  
Vol 1018 ◽  
Author(s):  
Kaoru Shoda ◽  
Seiji Takeda
Keyword(s):  
Electron Microscopy ◽  
Transmission Electron Microscopy ◽  
Photoelectron Spectroscopy ◽  
Amorphous Layer ◽  
Microscopy Study ◽  
Electron Microscopy Study ◽  
Rf Power ◽  
Multi Wall Carbon Nanotubes ◽  
Transmission Electron ◽  
Bonding Properties

AbstractVarious types of CNTs, i.e. single-wall, double-wall, triple-wall, quadruple-wall and multi-wall carbon nanotubes (CNTs), and fullerites were fluorinated in inductive coupled radio-frequency (RF) CF4 plasma at 13.56 MHz, and their structural and bonding properties were investigated by transmission electron microscopy (TEM) and X-ray photoelectron spectroscopy (XPS). We have discussed the correlation between the number of graphene sheets in a CNT and the stability against the fluorination. TEM and XPS analysis clearly state that increase of the number leads to the gain of fluorinated stability. The fluorination of CNTs is initiated at outer tubes and proceeds to inner tubes with increasing RF power, but fluorination depth is limited to only surface area. The fluorination of fullerites forms amorphous layer at the surface, and increases the depth of the layer with RF power.

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