Seed biopriming of durum wheat with purified and combined halotolerant bacteria improved seed germination under salinity conditions

Abstract Background The use of bioinoculants based on plant growth-promoting bacteria (PGPB) to promote plant growth under biotic and abiotic stresses is in full expansion. To our knowledge much work has not been, thus far, done on seed-biopriming of durum wheat for tolerance to biotic and abiotic stresses. In the present work, we report detailed account of the effectiveness a potent bacterial strain with proven plant growth-promoting ability and antimicrobial activity. The isolate was selected following screening of several bacterial strains isolated from halophytes that grow in a coastal saline soil in Tunisia for their role in enhancing durum wheat tolerance to both salinity stress and head blight disease.Results Accordingly, Bacillus strains MA9, MA14, MA17 and MA19 were found to have PGPB characteristics as they produced indole-3-acetic acid, siderophores and lytic enzymes, fixed free atmospheric nitrogen, and solubilized inorganic phosphate, in vitro . The in vivo study that involved in planta inoculation assays under control (25 mM NaCl) and stress (125 mM NaCl) conditions indicated that all PGPB strains significantly ( P < 0.05) increased the total plant length, dry weight, root area, seed weight, nitrogen, protein and total mineral content. On the other hand, strain MA17 reduced Fusarium Head Blight (FHB) disease incidence in wheat explants by 64.5%, showing that the strain has antifungal activity as was also displayed by in vitro inhibition study.Conclusions Both in vitro and in vivo studies showed that MA9, MA14 MA9, MA14, MA17 and MA19 strains were able to play the PGPB role. Yet, biopriming with Bacillus strain MA17 offered the highest bioprotection against FHB, plant growth promotion, and salinity tolerance. Hence, the MA17 strain should further be evaluated under field condition and formulated for commercial production. Besides, the strain could further be evaluated for its potential role in bioprotection and growth promotion of other crop plants. We believe, the strain has potential to significantly contribute to wheat production in the arid and semi-arid region, especially the salt affected Middle Eastern Region, besides its potential role in improving wheat production under biotic and abiotic stresses in other parts of the world.

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Durum Wheat Seed Germination Response to Hydrogel Coatings and Moisture under Drought Stress

American Journal of Agricultural and Biological Sciences ◽

10.3844/ajabssp.2016.67.75 ◽

2016 ◽

Vol 11 (2) ◽

pp. 67-75 ◽

Cited By ~ 3

Author(s):

Maya Hotta ◽

James Kennedy ◽

Clement Higginbotham ◽

Noreen Morris

Keyword(s):

Drought Stress ◽

Seed Germination ◽

Durum Wheat ◽

Wheat Seed ◽

Germination Response ◽

Hydrogel Coatings

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Effects of Osmotic Stress Induced by Polyethylene Glycol (Peg) 6000 and Mannitol on Seed Germination and Seedling Growth of Durum Wheat

Journal of Bioresource Management ◽

10.35691/jbm.1202.0195 ◽

2021 ◽

Vol 8 (3) ◽

pp. 57-66

Author(s):

Ratiba Bousba ◽

Rabah Bounar ◽

Narimene Sedrati ◽

Randa Lekhal ◽

Chourouk Hamla ◽

...

Keyword(s):

Polyethylene Glycol ◽

Seed Germination ◽

Durum Wheat ◽

Crop Production ◽

Germination Rate ◽

Inhibitory Effect ◽

Wheat Genotype ◽

Peg 6000 ◽

Germination Parameters

Seed germination is generally the critical step in seed establishment and thus the determination of successful crop production. This study was focused at examination of the biochemical and germination parameters effected by low water potential which was generated by polyethylene glycol (PEG) 6000 and mannitol, related to drought stress and growth of Waha durum wheat genotype. Two tests were carried out in a growth chamber; the first comprises seed germination into Petri dishes in the presence of different concentrations of the two osmoticums (0, 5, 10, 15 and 20 % of PEG6000 and mannitol). The second test was carried out in nutrient solution BD medium. Our results shows that Both PEG -6000 and mannitol reduced germination. Therefore, a rapid increase was observed in the rate of germination both for the control plants and the plants subjected to a concentration of 5 g/L and 10 g/L and changes in proportion to the time. For the concentration of 15 g/L and 20 g/L, this phase is very short, which explains the reduced germination rate due to the inhibitory effect of the two osmoticums on germination. In this study, PEG-6000 treatments resulted in an increase of some proteins and a decrease of others. Waha displayed 12 bands for control plants, 40 bands for PEG-6000 stressed plants (all treatments) and 35 bands for mannitol treatments.

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Coating seeds with Trichoderma strains promotes plant growth and enhance the systemic resistance against Fusarium crown rot in durum wheat

Egyptian Journal of Biological Pest Control ◽

10.1186/s41938-020-00338-6 ◽

2020 ◽

Vol 30 (1) ◽

Author(s):

Zayneb Kthiri ◽

Maissa Ben Jabeur ◽

Myriam Machraoui ◽

Samia Gargouri ◽

Khaled Hiba ◽

...

Keyword(s):

Seed Germination ◽

Durum Wheat ◽

Crop Production ◽

Growth Promotion ◽

Disease Incidence ◽

Crown Rot ◽

Systemic Resistance ◽

Main Body ◽

Seed Coating ◽

Fusarium Crown Rot

Abstract Background Fusarium crown rot is one of the major diseases that cause significant yield losses of wheat, and Trichoderma strains were known as an effective biocontrol agent. Main body The aim of this study was to evaluate the potential of coating durum wheat seeds of the cultivar “Karim” with 3 different Tunisian strains of Trichoderma sp. (S.INAT, SIO1, SIO2) and the Trichoderma-based commercial product Trianum-T22 on seed germination, seedling growth, and plant defense response against the pathogen Fusarium culmorum. The strains were identified using molecular tools based on sequencing ITS region of ribosomal DNA. The results confirmed at 99% of homology that the strains were T. harzianum. Under controlled conditions, the coating seeds were released with 400 μl of spore suspension at 107 spores/ml. The seed coating with Trianum-P, and S.INAT showed the highest seed germination rates ranging from 85 to 90% while S.IO1 and S.IO2 presented the lowest germination rates with 66 and 68%, respectively. At 20 days post-infection (dpi) with F. culmorum, the treated plants with S.INAT and Trianum-T22 reduced the disease incidence by 53.59 and 51.79%, respectively than the control. Besides, S.INAT induced two-folds the phenolic compounds level compared to infected control. Further, the peroxidase activity was enhanced by 50% in average since 10 dpi in plants treated with S.INAT and SIO2 than the control. Conclusion The results suggest that seed coating with T. harzianum S.INAT was a promising tool for crop production and protection under field conditions due to both direct antagonist activity and the indirect growth promotion. This strain seems to induce the systemic resistance of plants against foot crown rot disease.

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Seed biostimulant Bacillus sp. MGW9 improves the salt tolerance of maize during seed germination

AMB Express ◽

10.1186/s13568-021-01237-1 ◽

2021 ◽

Vol 11 (1) ◽

Author(s):

Heqin Li ◽

Haiwang Yue ◽

Li Li ◽

Yu Liu ◽

Haiyan Zhang ◽

...

Keyword(s):

Salt Tolerance ◽

Seed Germination ◽

Salinity Stress ◽

Sugar Content ◽

Soluble Sugar ◽

Primary Root ◽

Dry Weight ◽

Fresh Weight ◽

Acetic Acid Production ◽

Seed Biopriming

AbstractCrop performance is seriously affected by high salt concentrations in soils. To develop improved seed pre-sowing treatment technologies, it is crucial to improve the salt tolerance of seed germination. Here, we isolated and identified the strain Bacillus sp. MGW9 and developed the seed biostimulant MGW9. The effects of seed biopriming with the seed biostimulant MGW9 in maize (Zea mays L.) under saline conditions were studied. The results show that the strain Bacillus sp. MGW9 has characteristics such as salt tolerance, nitrogen fixation, phosphorus dissolution, and indole-3-acetic acid production. Seed biopriming with the seed biostimulant MGW9 enhanced the performance of maize during seed germination under salinity stress, improving the germination energy, germination percentage, shoot/seedling length, primary root length, shoot/seedling fresh weight, shoot/seedling dry weight, root fresh weight and root dry weight. Seed biostimulant MGW9 biopriming also alleviated the salinity damage to maize by improving the relative water content, chlorophyll content, proline content, soluble sugar content, root activity, and activities of superoxide dismutase, catalase, peroxidase and ascorbate peroxidase, while decreasing the malondialdehyde content. In particular, the field seedling emergence of maize seeds in saline-alkali soil can be improved by biopriming with the seed biostimulant MGW9. Therefore, maize seed biopriming with the seed biostimulant MGW9 could be an effective approach to overcoming the inhibitory effects of salinity stress and promoting seed germination and seedling growth.

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Seed Biostimulant MGW9 (SB-MGW9) Biopriming Improves Salt Tolerance during Maize Seed Germination

10.21203/rs.3.rs-266586/v1 ◽

2021 ◽

Author(s):

Heqin Li ◽

Haiwang Yue ◽

Li Li ◽

Yu Liu ◽

Haiyan Zhang ◽

...

Keyword(s):

Salt Tolerance ◽

Seed Germination ◽

Salinity Stress ◽

Soluble Sugar ◽

Dry Weight ◽

Strain Effect ◽

Bacillus Sp ◽

Maize Seed ◽

Fresh Weight ◽

Seed Biopriming

Abstract Crop performance is seriously affected by high salt concentrations in soils. To develop more new seed pre-sowing treatment technologies it is crucial to improve the salt tolerance of seed germination. Here we isolated and identified the strain Bacillus sp. MGW9 and developed the seed biostimulant MGW9 (SB-MGW9) by the strain. Effect of seed biopriming with SB-MGW9 in maize (Zea mays L.) under saline condition were studied. The results showed that the Bacillus sp. MGW9 has the characteristics of salt tolerance, nitrogen fixation, phosphorus dissolution, indole-3-acetic acid production and the like. Seed biopriming with SB-MGW9 enhanced the performance of maize during seed germination under salinity stress to improve the germination energy, germination percentage, shoot/seedling length, primary root length, shoot/seedling fresh weight, shoot/seedling dry weight, root fresh weight and root dry weight. SB-MGW9 biopriming also alleviates the salinity damage to maize by improving relative water content, chlorophyll content, proline content, soluble sugar content, root activity, activities of superoxide dismutase, catalase, peroxidase and ascorbate peroxidase, decreasing the malondialdehyde content. Especially, the field seedling emergence of maize seeds in saline-alkali soil can be improved by SB-MGW9 biopriming. Therefore, maize seed biopriming with SB-MGW9 can be an effective approach to resist the inhibitory effects of salinity stress and promote seed germination and seedling growth.

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Halotolerant bacteria with ACC deaminase activity alleviate salt stress effect in canola seed germination

Journal of the Korean Society for Applied Biological Chemistry ◽

10.1007/s13765-015-0025-y ◽

2015 ◽

Vol 58 (2) ◽

pp. 237-241 ◽

Cited By ~ 23

Author(s):

Md. Ashaduzzaman Siddikee ◽

Subbiah Sundaram ◽

Murugesan Chandrasekaran ◽

Kiyoon Kim ◽

Gopal Selvakumar ◽

...

Keyword(s):

Salt Stress ◽

Seed Germination ◽

Acc Deaminase ◽

Stress Effect ◽

Canola Seed ◽

Halotolerant Bacteria

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Durum wheat (Triticum durum Desf.) seed storage under controlled conditions

Genetičnì resursi roslin (Plant Genetic Resources) ◽

10.36814/pgr.2020.26.10 ◽

2020 ◽

pp. 105-115

Author(s):

O.A. Zadorozhna ◽

T. P. Shyianova ◽

M.Yu. Skorokhodov

Keyword(s):

Seed Germination ◽

Moisture Content ◽

Durum Wheat ◽

Germination Rate ◽

Seed Storage ◽

Seed Longevity ◽

Forest Steppe ◽

Controlled Conditions ◽

Seed Moisture ◽

Wheat Seeds

Aim. The aim of this work was to determine the longevity of durum wheat seeds according to the results of seed germination monitoring after seed storage for up to 19 years under controlled conditions in the National Plant Gene Bank of Ukraine. Results and Discussion. 36 accessions of spring durum wheat were investigated. These accessions belonged to var. hordeiforme, var. leucurum, var. melanopus, var. alexandrinum, var. apulicum, var. australe from seven countries; seven samples of durum winter wheat belonged to var. hordeiforme, var. leucurum were from Ukraine. Accessions were received by the Ukrainian genebank from seven countries: Ukraine, Russia, Mexico, France, Portugal, Kazakhstan and Tunisia. Seed accessions for storage were grown mainly in the eastern forest-steppe of Ukraine, stored in the National depositary in this region at unregulated temperature and at 4°C with seed moisture content of 5.5-8.0%. The mode of seed drying, which took place at temperature not higher than 25°C is discussed. The obtained results indicate high seed longevity of durum wheat under these conditions with initial seed germination rate more than 90% even in a storage facility at unregulated temperature. There were no differences in seed longevity between varieties of durum wheat under the studied storage conditions. Conclusions. Seeds of durum wheat remain unchanged for at least 10 years with a high initial germination of seeds, storage in sealed containers with moisture content 5.5-8.0% even at unregulated temperature of the eastern forest-steppe of Ukraine. Durum wheat seeds should be storage at temperature 4°C to increase seed longevity at seed moisture 5.5-8.0%.

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Indole-3-acetic acid (IAA) producing Pseudomonas isolates inhibit seed germination and α-amylase activity in durum wheat (Triticum turgidum L.)

Spanish Journal of Agricultural Research ◽

10.5424/sjar/2016141-8859 ◽

2016 ◽

Vol 14 (1) ◽

pp. e0802 ◽

Cited By ~ 12

Author(s):

Samira Tabatabaei ◽

Parviz Ehsanzadeh ◽

Hassan Etesami ◽

Hossein A. Alikhani ◽

Bernard R. Glick

Keyword(s):

Acetic Acid ◽

Seed Germination ◽

Durum Wheat ◽

Triticum Turgidum ◽

Amylase Activity ◽

Inhibitory Effect ◽

Bacterial Isolates ◽

Indole 3 Acetic Acid

The role of plant-associated bacteria in plant physiology and metabolism is well documented, but little has been known about the roles played by Pseudomonas in durum wheat (Triticum turgidum L. var durum) growth and development. An in vitroexperiment was conducted to observe the effect of the inoculation of four indole-3-acetic acid (IAA)-producing Pseudomonas isolates and exogenous IAA on seed germination traits and α-amylase activity of durum wheat. The results showed inoculation with all bacterial isolates led to a decrease in the germination percent, although the extent of the depression varied with the isolate. A significant relationship between concentrations of bacterial IAA and the germination inhibition percent in durum wheat seeds by different bacteria strains was observed. The results of this assay showed the effect of bacterial isolates on α-amylase activity after six and 8 days of inoculation was significant, while effect of these isolates on α-amylase activity after two and 4 days of inoculation was not meaningful. In addition, the exogenously applied IAA displayed a concentration-dependent effect on seed germination attributes and α-amylase activity, consistent with the possibility that the inhibitory effect of bacterial inoculation on seed germination was in consequence of bacteria-produced IAA. Therefore, it may suggested that the inhibitory role of IAA in seed germination and α-amylase activity should be taken into account during the screening of IAA-producing Pseudomonas isolates for durum wheat growth promoting agents.

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