Durum wheat (Triticum durum Desf.) seed storage under controlled conditions

Author(s):  
O.A. Zadorozhna ◽  
T. P. Shyianova ◽  
M.Yu. Skorokhodov

Aim. The aim of this work was to determine the longevity of durum wheat seeds according to the results of seed germination monitoring after seed storage for up to 19 years under controlled conditions in the National Plant Gene Bank of Ukraine. Results and Discussion. 36 accessions of spring durum wheat were investigated. These accessions belonged to var. hordeiforme, var. leucurum, var. melanopus, var. alexandrinum, var. apulicum, var. australe from seven countries; seven samples of durum winter wheat belonged to var. hordeiforme, var. leucurum were from Ukraine. Accessions were received by the Ukrainian genebank from seven countries: Ukraine, Russia, Mexico, France, Portugal, Kazakhstan and Tunisia. Seed accessions for storage were grown mainly in the eastern forest-steppe of Ukraine, stored in the National depositary in this region at unregulated temperature and at 4°C with seed moisture content of 5.5-8.0%. The mode of seed drying, which took place at temperature not higher than 25°C is discussed. The obtained results indicate high seed longevity of durum wheat under these conditions with initial seed germination rate more than 90% even in a storage facility at unregulated temperature. There were no differences in seed longevity between varieties of durum wheat under the studied storage conditions. Conclusions. Seeds of durum wheat remain unchanged for at least 10 years with a high initial germination of seeds, storage in sealed containers with moisture content 5.5-8.0% even at unregulated temperature of the eastern forest-steppe of Ukraine. Durum wheat seeds should be storage at temperature 4°C to increase seed longevity at seed moisture 5.5-8.0%.

Author(s):  
M.Yu. Skorokhodov ◽  
R.L. Boguslavskyi ◽  
V.S. Lyutenko

Аim. To determine the optimum moisture content and comparative longevity of seeds of wheat species accessions for long-term storage according to the results of the “accelerated seed aging” experiment. Results and Discussion. There was estimated seed longevity of the representatives of wheat species Triticum aestivum L., T. spelta L., T. durum Desf., T. dicoccum (Schrank) Schuebl., T. monococcum L., T. sinskajae A. Filat. et Kurk. of three reproduction years – 2014, 2016 and 2017, at three moisture levels – 5 %, 6 % and 7 % in the experiment with accelerated aging. Germination energy and germination rate depended on the year conditions, seed moisture, the interactions year – genotype, year – seed moisture and all three factors: year conditions, seed moisture, genotype (influence power from 16.1 to 20.1). Accelerated aging caused decrease in seed germination energy and germination rate in almost all samples at all three moisture levels. Under the accelerated aging, with an increase in seed moisture content from 5 % to 7 %, on average by the accessions, the seed germination energy (from 83 % to 63 %) and germination rate (from 90 % to 68 %) decreased on samples. At all three moisture levels, relatively high germination energy and germination rate after accelerated aging had T.monococcum (86-95 % and 88-97 %) and Polba 3 (74-96 % and 85-98 %). A low germination energy (66-69 % и 68-77 %) and germination rate showed T.sinskajae UA0300224 (66-69 %) and T.durum Spadschyna (56-75 % и 61-92 %). At all three seed moisture levels, the high plasticity of germination energy had T.sinskajae UA0300224 and T.durum Spadschina, germination rate – T.sinskajae. With all three seed moisture levels, T.sinskajae UA0300224 and T.aestivum Harkivska 26 were characterized by stable germination rate. Conclusions. The highest seed longevity of the studied accessions is provided by moisture of 5%, less but close longevity – the moisture of 6%. The least longevity in three test options had seeds with 7 % moisture content. Among studied accessions of wheat species most longevous seeds had T.monococcum UA0300439 and Polba 3. The seeds of T.sinskajae UA0300224 and T.astivum PI619379 were less longevous.


2021 ◽  
Vol 21 (07) ◽  
pp. 18368-18390
Author(s):  
Sipho Sibanda ◽  
◽  
S Mdlalose ◽  
T Workneh ◽  
M Laing ◽  
...  

The performance of a solar energy-assisted seed storage room was evaluated through an ordinary 22-m3 room that was retrofitted with a solar collector, inlets and chimney. The structure was made of a solar collector to heat the ambient air before entering the chimney. The chimney circulated the air inside the structure and inlets. To compare the performance of the modified storage room, a room with a similar capacity and without the retrofitted components (control storage room) was used. Twelve 8 kg bags of maize were stored in each storage room for a period of three months. Samples were taken every two weeks to determine germination rate, moisture content and seed vigour. The temperature and relative humidity (RH) was measured during storage. The RH in the control storage was significantly higher (P≤0.05) (60.6 ± 5.87%) than in the modified storage (40.1 ± 3.21%) during the day. However, at night, the RH in the control storage room was significantly lower (P≤0.05) (58.5 ± 7.32%) than in the modified storage (63.7 ± 6.28%). The RH in the modified storage room increased from 40.1% during the day to 63.7% at night. The RH in the control storage room decreased slightly from 60.6% to 58.5% during the day and night. The seed moisture content in the modified storage facility was significantly lower (P≤0.05) (12.6 ± 0.21%) than in the control storage room (13.3 ± 0.52%). The moisture content in the modified storage room decreased from 12.6% to 12.4%, whereas in the control room, moisture content increased from 12.6% to 13.8% in three months. The seed germination rate obtained after three months of storage in the modified storage room was significantly higher (P≤0.05) (98.5 ± 0.85%) than in the control storage room (96.8 ± 1.49%).The seed vigour obtained in the modified storage room was significantly higher (93.6 ± 0.35%) than in the control room (91.7 ± 2.08%) (P≤0.05). Seed stored in the control storage lost vigour at a faster rate, compared to the seeds stored in the modified storage room. Therefore, the modified naturally-ventilated seed storage room maintained seed quality better than the control storage room.


2019 ◽  
Vol 6 (2) ◽  
pp. 243-251
Author(s):  
M Anto ◽  
M Angala ◽  
P S Jothish ◽  
C Anilkumar

Garcinia imberti seeds were collected during 2015-2017 from Shangili, Cheenikkala and Bonaccord evergreen forests of Agasthyamala Biosphere Reserve, the only abode of this endangered endemic species. Germinability of seeds were analysed through decoating, Gibberellic acid (GA3) and light inductive pre-treatments on fresh (62.8 % moisture content; MC) and desiccated (fast; 23.3% MC and slow; 30.5 % MC) seeds. The seed germination with impermeable coat (0.7-1.2 mm) was restricted which on decoating got enhanced. Application of GA3 along with exposure to light breaked dormancy within 4-6 days compared to non-treated seeds that took 238-254 days to germinate. Stored seeds behaviour revealed that seed moisture content and rate of germination were negatively correlated. Seed storage was found to be more efficient only up to 80 days at controlled seed banking conditions (20 ± 20C, 20 % relative humidity; RH). Both fast and slow desiccated seeds stored for 60 days in seed bank conditions exhibited 50.4 and 43.4 % of germination compared 39.4% germination of non-desiccated seeds. Hence fast desiccated and decoated G. imberti seeds pre-treated with GA3 on subsequent exposure to light alleviated dormancy. For seed banking, fast desiccated seeds with MC in between 40-20% are found to be promising.


Silva Fennica ◽  
2021 ◽  
Vol 55 (3) ◽  
Author(s):  
Chuan Xie ◽  
Tianfeng Liu ◽  
Song Guo ◽  
Jian Peng ◽  
Zailiu Li

(H. Lév.) Rehder is a rare, endangered, and therefore, protected tree species native to China. However, there are serious limitations to the effective protection of the species, including a low seed germination-rate and difficult storage due to a high seed oil-content. Here, we evaluated the feasibility of ultra-dry seed storage and its effects on seedling growth. We used the silica gel method to prepare ultra-dry seeds with different moisture contents to find an optimal moisture content range (2.54%–4.77%). Ultra-dry treatment improved storability of . Furthermore, seeds with a moisture content of 4.77% stored at room temperature, and seeds with a moisture content of 3.97% stored at 4 °C yielded the best results. Priming with an appropriate concentration of polyethylene glycol had a certain repairing effect on ultra-dry stored seeds and improved seed vigor, with a two-day priming treatment with 20% polyethylene glycol having the best effect. Finally, compared with sand storage at 4 °C, ultra-dry storage promoted seedling growth and root development; furthermore, it alleviated storage damage to seeds, promoted soluble sugar and soluble protein accumulation, and increased seedling nitrogen, phosphorus, and potassium uptake. Therefore, ultra-dry storage can be effectively used to preserve seeds. Specifically, low-temperature storage of ultra-dry seeds with a moisture content of 3.97% enhanced seed vigor, and seedling growth and development.Handeliodendron bodinieriH. bodinieri seedsH. bodinieriH. bodinieriH. bodinieri


2004 ◽  
Vol 22 (1) ◽  
pp. 62
Author(s):  
A P Tyagi ◽  
M Taylor ◽  
P C Deo

Two taro (Colocasia esculenta (L.) Schott var. esculenta) cultivars from Fiji and Papua New Guinea were grown at the University of the South Pacific, Laucala Campus, Fiji to produce seeds for seed storage experiments. Gibberellic acid at a 500ppm concentration was used to induce flowering. Very few flowering shoots (inflorescence) were observed in the Fiji cultivar and all pollinations were unsuccessful. However the PNG cultivar flowered well and was used to obtain seed after hand pollination. Hand pollination was carried out to ensure seed setting in developing fruits in the inflorescence. Seeds were extracted in the laboratory after harvesting mature inflorescences. Experiments were conducted on seed moisture content, desiccation, germination, seedling development and seed storage behaviour of taro (Colocasia esculenta) seeds. Seed moisture content was determined using oven methods and air-drying. Results demonstrated that taro seeds have a moisture content of 12-13% after air-drying for three to four weeks. Seeds were dried to desired moisture contents in a desiccator over silica gel. After drying to 5% moisture content seed viability was tested by germinating seeds on moist filter paper at room temperature with 65% relative humidity and seven to eight hours day length. Preliminary seed germination tests demonstrated up to 83% germination for seeds with 13% moisture content. Germination occurred within five to seven days. Maximum germination was achieved within 21 days. The highest germination (80%) was achieved with seeds with 12% moisture content. Results indicated there was no relationship between moisture content and seed germination. Normal seedling development and growth was recorded after germination.


Author(s):  
OA Zadorozhna ◽  
TP Shyianova ◽  
MYu Skorokhodov ◽  
TM Shpak

Viability of rice (Orysa sativa L.) seeds accessions: subsp. japonica: var. italica, var. nigro-apiculata, var. subvulgaris; subsp. indica, var. mutica and var. fortuna was analised. The seed accessions were grown in the southern steppe of Ukraine and then stored at 4°C and −20°C. According to the research results, it is established that rice seeds in favorable cultivation years are able to maintain the original germination without significant changes for 10 years and longer with 6-8% moisture content even at 4°C storage temperature. There were no differences of rice seeds longevity of the same accessions stored at 4°C and −20°C. Differences of rice seed longevity of subsp. japonica and subsp. indica are discussed.


2016 ◽  
Vol 4 (4) ◽  
pp. 406-413
Author(s):  
Mohammad Ali JAHANBIN ◽  
◽  
Hasan Hüseyin GEÇİT ◽  
ÜNVER İKİNCİKARAKAYA ◽  
◽  
...  

2021 ◽  
Vol 27 (3) ◽  
pp. 162-166
Author(s):  
Abdul Azeez Hussain ◽  
◽  
Ramachandra Kurup Rajvikraman ◽  

Detailed study on seed storage and germination trailed in Knema attenuata (Wall. ex Hook. f. & Thomson) Warb.– the IUCN Red Listed ‘least concern’ medicinal tree species revealed that seeds were of recalcitrant nature. Viability of the seeds could be maintained for a longer period of up to 6 months with 47% Moisture content (mc) when kept in closed polycarbonate bottles at seed bank condition [20±20C Temp. and 40% Relative Humidity (RH)]. The 55% seed germination under normal climatic condition could be enhanced to a much higher percentage (75±5) inside the mist house chamber (34±30C Temp. and 70-80% RH).


2006 ◽  
Vol 16 (1) ◽  
pp. 83-88 ◽  
Author(s):  
Juvet Razanameharizaka ◽  
Michel Grouzis ◽  
Didier Ravelomanana ◽  
Pascal Danthu

The Adansonia (baobab) genus comprises seven species in Africa, six of which are endemic to Madagascar. Depending on the species, baobabs develop in widely varying ecosystems, including arid zones and savannahs, as well as dry and wet forests. Seeds from all species exhibited orthodox behaviour, tolerating dehydration to a moisture content of around 5%. There was no physical dormancy in the two species belonging to the Brevitubae section, A. grandidieri and A. suarezensis. Their seeds germinated without any prior scarification. The five other species, belonging to Adansonia and Longitubae section, have seeds with water-impermeable coats. In the case of A. digitata and A. za, the proportion of water-impermeable seeds was around two-thirds, whereas with A. rubrostipa, A. madagascariensis and A. perrieri, the proportion was >90%. Treatments allowing for the removal of physical dormancy needed to be markedly more severe with A. madagascariensis than with the other species. None the less, it seems impossible to link these characteristics and the interspecific differences to a strategy for adaptation by these species to their environment.


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