scholarly journals Bioactive Novel Indole Alkaloids and Steroids from Deep Sea-Derived Fungus Aspergillus fumigatus SCSIO 41012

Molecules ◽  
2018 ◽  
Vol 23 (9) ◽  
pp. 2379 ◽  
Author(s):  
Salendra Limbadri ◽  
Xiaowei Luo ◽  
Xiuping Lin ◽  
Shengrong Liao ◽  
Junfeng Wang ◽  
...  
Keyword(s):  
Mass Spectrometry ◽  
Nuclear Magnetic Resonance ◽  
Aspergillus Fumigatus ◽  
Deep Sea ◽  
Spectroscopic Data ◽  
Indole Alkaloids ◽  
Significant Activity ◽  
Inhibitory Activities ◽  
Antibacterial And Antifungal ◽  
Mic Value

Two new alkaloids, fumigatosides E (1) and F (2), and a new natural product, 3, 7-diketo-cephalosporin P1 (6) along with five known compounds (3–5, 7, 8) were isolated from deep-sea derived fungal Aspergillus fumigatus SCSIO 41012. Their structures were determined by extensive spectroscopic data analysis, including 1D, 2D nuclear magnetic resonance (NMR) and mass spectrometry (MS), and comparison between the calculated and experimental electronic circular dichroism (ECD) spectra. In addition, all compounds were tested for antibacterial and antifungal inhibitory activities. Compound 1 showed significant antifungal activity against Fusarium oxysporum f. sp. momordicae with MIC at 1.56 µg/mL. Compound 4 exhibited significant higher activity against S. aureus (16,339 and 29,213) with MIC values of 1.56 and 0.78 µg/mL, respectively, and compound 2 exhibited significant activity against A. baumanii ATCC 19606 with a MIC value of 6.25 µg/mL.

scholarly journals A new tetracyclic diterpenoid from the seeds of Euphorbia lathyris

2020 ◽  
Vol 44 (5-6) ◽  
pp. 322-325
Author(s):  
Lie-Feng Ma ◽  
Yue Zhang ◽  
Xuan Zhang ◽  
Meng-Jia Chen ◽  
Zha-Jun Zhan ◽  
...  
Keyword(s):  
Mass Spectrometry ◽  
Nuclear Magnetic Resonance ◽  
High Resolution ◽  
Spectroscopic Data ◽  
Resonance Spectroscopy ◽  
Ionization Mass ◽  
Two Dimensional ◽  
Detailed Interpretation ◽  
A Minor ◽  
Euphorbia Lathyris

Eupholathone, a minor diterpenoid with an unusual tetracyclic skeleton, was obtained from the seeds of Euphorbia lathyris, along with two known lathyrane diterpenoids, euphorbia factors L2 and L3. The structure of eupholathone was elucidated by detailed interpretation of its spectroscopic data, especially two-dimensional nuclear magnetic resonance spectroscopy and high-resolution electrospray ionization mass spectrometry.

scholarly journals Two New AChE Inhibitors Isolated from Li Folk Herb Heilaohu “Kadsura coccinea” Stems

Molecules ◽  
2019 ◽  
Vol 24 (19) ◽  
pp. 3628
Author(s):  
Sheng Zhuo Huang ◽  
Lin Ping Duan ◽  
Hao Wang ◽  
Wen Li Mei ◽  
Hao Fu Dai
Keyword(s):  
Mass Spectrometry ◽  
Nuclear Magnetic Resonance ◽  
Magnetic Resonance ◽  
Spectroscopic Data ◽  
Positive Control ◽  
Ache Inhibitors ◽  
Activity Test ◽  
Ache Inhibitory Activity ◽  
Kadsura Coccinea ◽  
Ellman’S Method

Two new triterpenoids, named kadsuricoccins A and B, together with three known ones, were isolated from the Li folk herb Heilaohu, the stems of Kadsura coccinea (Lem.) A. C. Smith, which was used for food and as a healthy supplement. Their structures were elucidated by comprehensive analyses of mass spectrometry (MS) and nuclear magnetic resonance (NMR) spectroscopic data. To search healthy components, an acetylcholinesterase (AChE) inhibitory activity test by Ellman’s Method was conducted, kadsuricoccins A and B showed activity with the AChE inhibit index (AII) up to 68.96% ± 0.19% and 57.8% ± 0.11% at 94 nM (compared with positive control tacrine AII 79.80% ± 0.20%, 9.4 nM), respectively.

scholarly journals Antimicrobial Lavandulylated Flavonoids from a Sponge-Derived Streptomyces sp. G248 in East Vietnam Sea

Marine Drugs ◽  
2019 ◽  
Vol 17 (9) ◽  
pp. 529 ◽  
Author(s):  
Cao ◽  
Trinh ◽  
Mai ◽  
Vu ◽  
Le ◽  
...  
Keyword(s):  
Mass Spectrometry ◽  
Nuclear Magnetic Resonance ◽  
Antimicrobial Activity ◽  
Mycobacterium Tuberculosis ◽  
Spectroscopic Data ◽  
Inhibitory Concentration ◽  
Culture Broth ◽  
Ionization Mass ◽  
Streptomyces Sp ◽  
Mycobacterium Tuberculosis H37rv

Three new lavandulylated flavonoids, (2S,2′’S)-6-lavandulyl-7,4′-dimethoxy-5,2′-dihydroxylflavanone (1), (2S,2′’S)-6-lavandulyl-5,7,2′,4′-tetrahydroxylflavanone (2), and (2′’S)-5′-lavandulyl-2′-methoxy-2,4,4′,6′-tetrahydroxylchalcone (3), along with seven known compounds 4–10 were isolated from culture broth of Streptomyces sp. G248. Their structures were established by spectroscopic data analysis, including 1D and 2D nuclear magnetic resonance (NMR), and high-resolution electrospray ionization mass spectrometry (HR-ESI-MS). The absolute configurations of 1–3 were resolved by comparison of their experimental and calculated electronic circular dichroism spectra. Compounds 1–3 exhibited remarkable antimicrobial activity. Whereas, two known compounds 4 and 5 exhibited inhibitory activity against Mycobacterium tuberculosis H37Rv with minimum inhibitory concentration (MIC) values of 6.0 µg/mL and 11.1 µg/mL, respectively.

scholarly journals Neo-Aplysiatoxin A Isolated from Okinawan Cyanobacterium Moorea Producens

Molecules ◽  
2020 ◽  
Vol 25 (3) ◽  
pp. 457 ◽  
Author(s):  
Mioko Kawaguchi ◽  
Masayuki Satake ◽  
Bo-Tao Zhang ◽  
Yue-Yun Xiao ◽  
Masayuki Fukuoka ◽  
...  
Keyword(s):  
Mass Spectrometry ◽  
Nuclear Magnetic Resonance ◽  
Magnetic Resonance ◽  
High Resolution ◽  
Growth Inhibition ◽  
Spectroscopic Data ◽  
High Resolution Mass Spectrometry ◽  
High Resolution Mass ◽  
Resolution Mass ◽  
Nuclear Magnetic

A new aplysiatoxin derivative, neo-aplysiatoxin A (1), along with seven known compounds, neo-debromoaplysiatoxin A (2), dolastatin 3 (3), lyngbic acid (4), malyngamide M (5), hermitamide A (6), (−)-loliolide (7), and (+)-epiloliolide (8), was isolated from the Okinawan cyanobacterium Moorea producens. Their structures were elucidated on the basis of spectroscopic data, including high-resolution mass spectrometry and nuclear magnetic resonance. The compounds were evaluated for cytotoxic and diatom growth inhibition activities.

scholarly journals Anti-Acetylcholinesterase Compounds Isolated from the Leaves of Kigelia africana (LAM) Benth (Bignoniaceae)

2019 ◽  
pp. 1-9 ◽  
Author(s):  
John A. Falode ◽  
Olamide O. Crown ◽  
Samson O. Famuyiwa ◽  
Christianah A. Elusiyan ◽  
Ifedayo V. Ogungbe ◽  
...  
Keyword(s):  
Mass Spectrometry ◽  
Nuclear Magnetic Resonance ◽  
Ethyl Acetate ◽  
Spectroscopic Data ◽  
Ache Activity ◽  
Treatment Strategies ◽  
Ache Inhibition ◽  
Structural Activity Relationship ◽  
Vacuum Liquid Chromatography ◽  
Relationship Of

Acetylcholinesterase (AChE) is an enzyme that is involved in the breakdown of some neurotransmitters. Its inhibition is one of the treatment strategies employed in the management Alzheimer diseases. Flavonoids isolated from the leaves of Kigelia africana were investigated for their comparative AChE inhibition. The extract of the leaves was subjected to vacuum liquid chromatography (VLC) to obtain four fractions using n-hexane (n-hex, 100%), n-hexane/dichloromethane (hex/DCM, 1:1), dichloromethane/ethyl acetate (DCM/EtOAc, 1:1) and ethyl acetate/methanol (EtOAc/MeOH, 1:1). The four fractions were subjected to AChE inhibitory study with DCM/EtOAc (1:1) fraction showing the highest inhibitory activity. Three flavonoids were isolated from this fraction and their structures were elucidated and characterised using 1D- and 2D-nuclear magnetic resonance (NMR) spectroscopy and mass spectrometry (MS) techniques. Their spectroscopic data compared well with literature. The compounds demonstrated considerable inhibition of AChE activity with luteolin (1), rutin (2) and quercetin (3) that showed IC50 of 945.0, 282.1, 254.8 μg/ml respectively as against the IC50 of 38.93 μg/ml for rivastigmine, a well-known cholinesterase agent. Compound 3 showed 17.89 ± 0.57 and 7.70 ± 0.64 μ/l/mg protein at 200 and 400 μg/ml respectively, for AChE activity as against 10.37 ± 0.99 and 6.24 ± 1.24 μ/l/mg protein showed by rivastigmine at 200 and 400 μg/ml respectively. This study showed that the phytochemical responsible for the AChE inhibition in the crude extract as reported by Falode et al., 2017 resided in the DCM/EtOAc (1:1) fraction. The structural-activity relationship of the flavonoids revolves around substitution in position 3 of the compounds. 

EXTRACTION, ISOLATION, CHARACTERIZATION, SEMISYNTHESIS AND BIOLOGICAL EVALUATION OF BERBERINE FROM ROOTS OF BERBERIS ARISTATA

INDIAN DRUGS ◽  
2013 ◽  
Vol 50 (09) ◽  
pp. 18-24
Author(s):  
J Mittal ◽  
◽  
K. Chaudhary ◽  
N Kumar ◽  
D Pathak
Keyword(s):  
Mass Spectrometry ◽  
Elemental Analysis ◽  
Biological Evaluation ◽  
Diffusion Method ◽  
Significant Activity ◽  
Bacterial Strains ◽  
Percolation Method ◽  
Acetophenone Derivatives ◽  
Antibacterial And Antifungal

A methanolic extract of roots of Berberis aristata was prepared by using hot percolation method. Berberine was isolated from the above extract and characterized by IR, 1HNMR, mass spectrometry and elemental analysis. A series of 8-(substituted acetophenone) berberine derivatives was synthesized by introducing various acetophenone derivatives at 8th position of berberine and analyzed by melting point and TLC. The structures of all the synthesized compounds were characterized by IR, 1HNMR, mass spectrometry and elemental analysis. The semisynthesized compounds were screened for in vitro antibacterial and antifungal activity using disc (5mm) diffusion method on nutrient agar medium against Gram-positive (Staphylococcus aureus and Bacillus subtilis), Gram-negative bacterial strains (Pseudomonas aeruginosa and Escherichia coli) and fungal strains (Aspergillus niger and Candida albicans) respectively. The zone of inhibition was compared with standard drugs berberine and kanamycin. All the above compounds showed significant activity in comparison to the standards berberine and kanamycin.

scholarly journals Monoterpene Indole Alkaloids from the Twigs of Kopsia arborea

2014 ◽  
Vol 9 (10) ◽  
pp. 1934578X1400901
Author(s):  
Sarot Cheenpracha ◽  
Achara Raksat ◽  
Thunwadee Ritthiwigrom ◽  
Surat Laphookhieo
Keyword(s):  
Antibacterial Activity ◽  
Nmr Spectroscopy ◽  
Indole Alkaloids ◽  
2D Nmr ◽  
Positive Control ◽  
Ache Inhibition ◽  
2D Nmr Spectroscopy ◽  
E Coli ◽  
Inhibitory Activities ◽  
Mic Value

The phytochemistry of Kopsia arborea Blume has received considerable attention, which has resulted in the isolation of a number of new unusual indole alkaloids with intriguing structures. In this study, a new eburnane-type alkaloid, phutdonginin (1), together with eight known alkaloids: 19-OH-(-)-eburnamonine (2), melodinine E (3), kopsinine (4), kopsilongine (5), kopsamine (6), (-)-methylenedioxy-11,12-kopsinaline (7), decarbomethoxykopsiline (8), and vincadifformine (9), were isolated from the twigs of K. arborea. Their structures were characterized extensively by 1D and 2D NMR spectroscopy and HR-ESI-MS. All compounds were submitted to TLC screening for acetylcholinesterase inhibitory activities. Only kopsamine and decarbomethoxykopsiline showed AChE inhibition with MIR values of 12.5 and 6.25 μg, respectively, compared with galanthamine (positive control, 0.004 μg). In addition, compounds 1 and 2 inhibited moderate antibacterial activity against E. coli TISTR 780 with the MIC value of 32 μg/mL.

scholarly journals Cytotoxic Sesquiterpenoids From Soft Soral Capnella imbricata

2019 ◽  
Vol 14 (6) ◽  
pp. 1934578X1985749
Author(s):  
Chin-Soon Phan ◽  
Takashi Kamada ◽  
Takahiro Ishii ◽  
Toshiyuki Hamada ◽  
Charles Santhanaraju Vairappan
Keyword(s):  
Mass Spectrometry ◽  
Nuclear Magnetic Resonance ◽  
Magnetic Resonance ◽  
High Resolution ◽  
Spectroscopic Data ◽  
Soft Coral ◽  
Inhibitory Concentration ◽  
Ionization Mass ◽  
Cell Leukemia ◽  
Adult T Cell Leukemia

A new sesquiterpene, capgermacrene H (1) was isolated together with 7 other compounds, capgermacrenes A-G (2-8), from a population of soft coral Capnella imbricata collected from Mantanani Island, Sabah, Malaysia. The structure of this metabolite was elucidated based on spectroscopic data such as nuclear magnetic resonance and high-resolution electrospray ionization mass spectrometry. Capgermacrene A (2) showed potent cytotoxicity against adult T-cell leukemia, S1T cells at half-maximal inhibitory concentration of 0.79 µg/mL.

scholarly journals Chemical Composition and Enzyme Inhibitory Activities of Turkish Pancratium maritimum Bulbs

2019 ◽  
Vol 14 (10) ◽  
pp. 1934578X1987290 ◽  
Author(s):  
Buket Bozkurt ◽  
Gulen I. Kaya ◽  
Nehir U. Somer
Keyword(s):  
Mass Spectrometry ◽  
Gas Chromatography ◽  
Chemical Composition ◽  
Inhibitory Activity ◽  
Indole Alkaloids ◽  
Gas Chromatography Mass Spectrometry ◽  
Prolyl Oligopeptidase ◽  
Amaryllidaceae Alkaloids ◽  
Inhibitory Activities ◽  
Pancratium Maritimum

The chemical composition of the bulbs of Pancratium maritimum L. (Amaryllidaceae) from Turkey (Pamucak, Aydın) has been determined by gas chromatography-mass spectrometry. A total of 29 compounds belonging to different skeletal types of Amaryllidaceae alkaloids were identified. Lycorine, galanthamine, crinine, and pancracine were found as major constituents. Interestingly, indole alkaloids (1-acetyl-β -carboline and galanthindole) were also detected. Acetylcholinesterase, butyrylcholinesterase, and prolyl oligopeptidase inhibitory activity potentials of the alkaloidal extract were also determined. The results of the present study show that Turkish P. maritimum comprises a rich repository for bioactive alkaloids with intriguing structural diversities.

scholarly journals Three New Quinazoline-Containing Indole Alkaloids From the Marine-Derived Fungus Aspergillus sp. HNMF114

2021 ◽  
Vol 12 ◽  
Author(s):  
Sha-Sha Liu ◽  
Li Yang ◽  
Fan-Dong Kong ◽  
Jia-Hui Zhao ◽  
Li Yao ◽  
...  
Keyword(s):  
Nuclear Magnetic Resonance ◽  
Spodoptera Frugiperda ◽  
Quantum Chemical ◽  
Spectroscopic Data ◽  
Chemical Shifts ◽  
Indole Alkaloids ◽  
Amide Group ◽  
Activation Effect ◽  
Aspergillus Sp ◽  
New Compounds

By feeding tryptophan to the marine-derived fungus Aspergillus sp. HNMF114 from the bivalve mollusk Sanguinolaria chinensis, 3 new quinazoline-containing indole alkaloids, named aspertoryadins H–J (1–3), along with 16 known ones (4–19), were obtained. The structures of the new compounds were elucidated by the analysis of spectroscopic data combined with quantum chemical calculations of nuclear magnetic resonance (NMR) chemical shifts and electron capture detector (ECD) spectra. Structurally, compound 3 represents the first example of this type of compound, bearing an amide group at C-3. Compounds 10 and 16 showed potent α-glucosidase inhibitory activity with IC50 values of 7.18 and 5.29 μM, and compounds 13 and 14 showed a clear activation effect on the ryanodine receptor from Spodoptera frugiperda (sfRyR), which reduced the [Ca2+]ER by 37.1 and 36.2%, respectively.

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