scholarly journals Hydrogen Production from Energy Poplar Preceded by MEA Pre-Treatment and Enzymatic Hydrolysis

Molecules ◽  
2018 ◽  
Vol 23 (11) ◽  
pp. 3029 ◽  
Author(s):  
Karolina Kucharska ◽  
Rafał Łukajtis ◽  
Edyta Słupek ◽  
Hubert Cieśliński ◽  
Piotr Rybarczyk ◽  
...  
Keyword(s):  
Enzymatic Saccharification ◽  
Enterobacter Aerogenes ◽  
Dark Fermentation ◽  
Batch Reactors ◽  
Alkaline Catalyst ◽  
Variable Parameters ◽  
Box Behnken Design ◽  
Concentration Time ◽  
Treatment Conditions ◽  
Pre Treatment

The need to pre-treat lignocellulosic biomass prior to dark fermentation results primarily from the composition of lignocellulose because lignin hinders the processing of hard wood towards useful products. Hence, in this work a two-step approach for the pre-treatment of energy poplar, including alkaline pre-treatment and enzymatic saccharification followed by fermentation has been studied. Monoethanolamine (MEA) was used as the alkaline catalyst and diatomite immobilized bed enzymes were used during saccharification. The response surface methodology (RSM) method was used to determine the optimal alkaline pre-treatment conditions resulting in the highest values of both total released sugars (TRS) yield and degree of lignin removal. Three variable parameters (temperature, MEA concentration, time) were selected to optimize the alkaline pre-treatment conditions. The research was carried out using the Box-Behnken design. Additionally, the possibility of the re-use of both alkaline as well as enzymatic reagents was investigated. Obtained hydrolysates were subjected to dark fermentation in batch reactors performed by Enterobacter aerogenes ATCC 13048 with a final result of 22.99 mL H2/g energy poplar (0.6 mol H2/mol TRS).

Optimisation of pre-treatment conditions for plantain (Musa parasidiaca) flour using Box-Behnken design

2018 ◽  
Vol 10 (3) ◽  
pp. 223-232
Author(s):  
G.J. Fadimu ◽  
L.O. Sanni ◽  
A.A. Adebowale ◽  
S.O. Kareem ◽  
O.P. Sobukola ◽  
...  
Keyword(s):  
Box Behnken Design ◽  
Treatment Conditions ◽  
Pre Treatment

Comparative study of the efficiency of pretreatment with alkaline hydrogen peroxide in pineapple bagasse in different granulometries submitted to acid and enzymatic saccharification

2021 ◽  
Vol 10 (1) ◽  
pp. e4921019902
Author(s):  
Danielle Pires Nogueira ◽  
Lorena Costa Vasconcelos ◽  
Gabriel Luis Castiglioni ◽  
Fernanda Ferreira Freitas ◽  
Araceli Aparecida Seolatto
Keyword(s):  
Hydrogen Peroxide ◽  
Enzymatic Saccharification ◽  
Raw Material ◽  
Particle Sizes ◽  
Alkaline Hydrogen Peroxide ◽  
Concentration Time ◽  
Total Reducing Sugars ◽  
Pre Treatment ◽  
High Concentrations ◽  
Acid Saccharification

This work had the purpose of evaluating the efficiency of the pretreatment with alkaline hydrogen peroxide of pineapple bagasse in order to obtain fermentable sugars by applying acid and enzymatic hydrolysis to said residue. Four experimental designs were applied to study the best conditions for the pre-treatment. Total reducing sugars (TRS) concentration was the response and hydrogen peroxide concentration, time and temperature were the independent variables. The studies were conducted using pineapple bagasse with particle sizes of 20 mesh and 48 mesh. Acid saccharification, with 2.9% sulfuric acid (v/v), following the pre-treatment, yielded TRS concentrations that reached 0.094 g of TRS/g of raw bagasse for 20 mesh and 0.101 g of TRS/g of raw bagasse for 48 mesh. The enzymatic saccharification, with 9 FPU/g cellulase and 2% (m/v) of bagasse, reached 0.063 g of TRS/g of raw bagasse for both particle sizes. The peroxide concentration showed a significant influence, the use of high concentrations reduced the TRS output in both hydrolysis. With the results found in this work, it is possible to infer the feasibility of applying pineapple bagasse as a lignocellulosic raw material. 

scholarly journals Exogenous 5-azaCitidine accelerates flowering and external GA3 increases ornamental value in Iranian Anemone accessions

2021 ◽  
Vol 11 (1) ◽  
Author(s):  
Vahideh Yari ◽  
Zeynab Roein ◽  
Atefeh Sabouri
Keyword(s):  
Morphological Characteristics ◽  
Flower Longevity ◽  
Cut Flowers ◽  
Stem Height ◽  
Flowering Stage ◽  
Potted Plants ◽  
Dormancy Period ◽  
Treatment Conditions ◽  
Number Of Leaves ◽  
Pre Treatment

AbstractThe Anemone genus is a tuberous geophyte which undergoes a dormancy period during unfavorable environmental conditions for growth. Five species of the Anemone genus naturally grow in several regions of Iran. The diverse uses of Anemone in gardens for landscaping, cut flowers, and potted plants indicate its high ornamental potential. Its dormancy and flowering are influenced by various factors. The present paper was conducted to explore the flowering behavior of Anemone accessions in response to different pre-treatments. For this purpose, tubers of 18 Anemone accessions (A. coronaria and A. biflora) were collected from natural regions of six provinces in Iran. These tubers were subjected to different conditions of non-chilling (20 °C, 90 days), chilling (4 °C, 90 days), GA3 (150 mgL-1; 24 h), and 5-azaCitidine (5-azaC; 40 µM; 24 h) prior to the cultivation. Most of the accessions were able to enter the flowering stage without chilling. The shortest period for the sprouting of tubers (16.89 ± 7.83 days) belonged to 5-azaC pre-treatment. In addition, this treatment accelerated the flowering time (about 30 days earlier) and diameter of the stem, bud, and flower. Morphological characteristics, such as stem height, number of leaves, bud, and petal and the longevity of flowers on the plant were significantly affected by GA3 pre-treatment. Our results indicated a positive correlation between flower length, stem height, and stem diameter with flower longevity under different pre-treatment conditions. The present study demonstrated that accessions Anm3, Anm12, and Anm18 had ornamental values higher than the population mean across four conditions.

scholarly journals Optimization of oxalic acid pre-treatment and enzymatic saccharification in Typha latifolia for production of reducing sugar

2020 ◽  
Vol 18 (1) ◽  
Author(s):  
Sunil Kodishetty Ramaiah ◽  
Girisha Shringala Thimappa ◽  
Lokesh Kyathasandra Nataraj ◽  
Proteek Dasgupta
Keyword(s):  
Oxalic Acid ◽  
Enzymatic Saccharification ◽  
Typha Latifolia ◽  
Reducing Sugar ◽  
Pre Treatment

Propionic acid production from food waste in batch reactors: Effect of pH, types of inoculum, and thermal pre-treatment

2021 ◽  
Vol 319 ◽  
pp. 124166
Author(s):  
Rowayda Ali ◽  
Florencia Saravia ◽  
Andrea Hille-Reichel ◽  
Johannes Gescher ◽  
Harald Horn
Keyword(s):  
Propionic Acid ◽  
Food Waste ◽  
Acid Production ◽  
Batch Reactors ◽  
Effect Of Ph ◽  
Propionic Acid Production ◽  
Pre Treatment

scholarly journals Synthesis of Bio-Oilphenol-Formaldehyde Resins under Alkali Conditions

2020 ◽  
Vol 71 (1) ◽  
pp. 19-27
Author(s):  
Günay Özbay ◽  
Caglar Cekic ◽  
Muhammad Syarhabil Ahmad ◽  
Erkan Sami Kokten
Keyword(s):  
Shear Strength ◽  
Phenol Formaldehyde ◽  
Bonding Performance ◽  
Performance Requirements ◽  
Vacuum Pyrolysis ◽  
Treatment Conditions ◽  
Bio Oil ◽  
Dry Conditions ◽  
Ft Ir ◽  
Pre Treatment

In the present study, bio-oil produced from vacuum pyrolysis of woody biomass has been investigated as a source of chemical feedstock. Bio-based resins were produced using the bio- oil with phenol substitutions ranging from 10 to 30 wt%. The conventional GC/MS analysis was carried out for the evaluation of the chemical composition of bio-oil. TGA, DSC and FT-IR analyses were used in order to characterize the bio-oil-phenol-formaldehyde (BPF) resins. The bonding quality of wood samples bonded with the BPF resins was investigated under different pre-treatment conditions. The highest shear strength was observed for the control samples bonded with the laboratory PF resin. As the amount of bio-oil was increased up to 30 wt%, the shear strength of the samples decreased from 12.08 to 11.76 N/mm2. The bonding performance was not negatively affected by the combination of bio-oil under dry conditions. According to TS EN 12765 standard, the relevant performance requirements for bonded samples under dry conditions must be at least 10 N/mm2. Relating to the standard, all samples bonded with BPF resins obtained the requirements for durability class C1. Under wet conditions, the bonding performance was negatively affected by the addition of bio-oil. However, the BPF resins fulfilled the durability requirements for C1, C2, and C3 specified in EN 12765 (2002).

scholarly journals Bioethanol Production from UK Seaweeds: Investigating Variable Pre-treatment and Enzyme Hydrolysis Parameters

2019 ◽  
Vol 13 (1) ◽  
pp. 271-285 ◽  
Author(s):  
Emily T. Kostas ◽  
Daniel A. White ◽  
David J. Cook
Keyword(s):  
Large Scale ◽  
Method Development ◽  
Reaction Times ◽  
Enzyme Hydrolysis ◽  
Bioethanol Production ◽  
Laminaria Digitata ◽  
Treatment Conditions ◽  
Water Based ◽  
Ethanol Yields ◽  
Pre Treatment

Abstract This study describes the method development for bioethanol production from three species of seaweed. Laminaria digitata, Ulva lactuca and for the first time Dilsea carnosa were used as representatives of brown, green and red species of seaweed, respectively. Acid thermo-chemical and entirely aqueous (water) based pre-treatments were evaluated, using a range of sulphuric acid concentrations (0.125–2.5 M) and solids loading contents (5–25 % [w/v]; biomass: reactant) and different reaction times (5–30 min), with the aim of maximising the release of glucose following enzyme hydrolysis. A pre-treatment step for each of the three seaweeds was required and pre-treatment conditions were found to be specific to each seaweed species. Dilsea carnosa and U. lactuca were more suited with an aqueous (water-based) pre-treatment (yielding 125.0 and 360.0 mg of glucose/g of pre-treated seaweed, respectively), yet interestingly non pre-treated D. carnosa yielded 106.4 g g−1 glucose. Laminaria digitata required a dilute acid thermo-chemical pre-treatment in order to liberate maximal glucose yields (218.9 mg glucose/g pre-treated seaweed). Fermentations with S. cerevisiae NCYC2592 of the generated hydrolysates gave ethanol yields of 5.4 g L−1, 7.8 g L−1 and 3.2 g L−1 from D. carnosa, U. lactuca and L. digitata, respectively. This study highlighted that entirely aqueous based pre-treatments are effective for seaweed biomass, yet bioethanol production alone may not make such bio-processes economically viable at large scale.

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