scholarly journals Effect of High-Pressure Processing (HPP) on the Fatty Acid Profile of Different Sized Ragworms (Hediste diversicolor) Cultured in an Integrated Multi-Trophic Aquaculture (IMTA) System

Molecules ◽  
2019 ◽  
Vol 24 (24) ◽  
pp. 4503 ◽  
Author(s):  
Bruna Marques ◽  
Ana Isabel Lillebø ◽  
Maria do Rosário M. Domingues ◽  
Jorge A. Saraiva ◽  
Ricardo Calado

Ragworms (Hediste diversicolor) cultured under integrated multi-trophic aquaculture (IMTA) conditions display an improved fatty acids (FA) profile than conspecifics from the wild, thus being more suitable for maturation diets of marine fish and shrimp. Nonetheless, their use may represent a potential pathway for pathogens. The objective of the present study was to determine if high-pressure processing (HPP), as an approach to safeguard microbiological safety, could promote significant shifts on the FA profiles of different sized ragworms. An analysis of similarities (ANOSIM) revealed the existence of significant differences in the FA profile and lipid quality indexes (atherogenicity (AI), thrombogenicity (TI) and polyene (PI)) of control and HPP treated ragworms of all tested sizes (small, medium and large). Saturated (SFA) and monounsaturated FA (MUFA) increased after HPP, while polyunsaturated FA (PUFA; FA with 2 or 3 double bonds) and highly unsaturated FA (HUFA; FA with ≥ 4 double bonds) decreased. The amount of docosahexaenoic acid (DHA) in polychaetes exposed to HPP decreased an average of 25%, when compared with the levels recorded in control groups. The values of PI significantly decreased after HPP, while those of AI and TI displayed a significant increase. Despite the shifts in the FA profile of ragworms exposed to HPP, these still display a superior profile to that of wild specimens, namely the presence of DHA. Therefore, HPP can be considered as a suitable approach to safeguard the biosecurity of cultured polychaetes, without compromising their nutritional value, and support the principles of circular economy through the use of IMTA.

Author(s):  
Roberta Stefanini ◽  
Giuseppe Vignali ◽  
Fabio Coloretti

Today consumers demand fresh foods without additives, preservatives and health risks: that is why non-thermal food preservation methods are receiving more interest, among them High Pressure Processing is able to avoid thermal degradation of food components, extend their shelf life and preserve colour, flavour and nutritional value. HPP is often used on dairy products because of its impact on physicochemical and sensory characteristics, its ability to improve their structure and texture and inactivate some microorganisms. The aim of this work is to evaluate the effect of HPP on a packaged ricotta rich in Conjugated Linoleic Acid (CLA) and Omega-3, resulting from cows fed with linseed in the Parmigiano Reggiano area, and processed with a hydrostatic pressure of 600 MPa for 5 minutes. The ultimate goal is to find a mathematical model able to show the treatment’s effect on spoilage microorganisms that grow spontaneously in this product during a month of refrigerated storage.


2022 ◽  
Vol 10 (1) ◽  
pp. 59
Author(s):  
Svetlana A. Murzina ◽  
Viktor P. Voronin ◽  
Tatjana R. Ruokolainen ◽  
Dmitrii V. Artemenkov ◽  
Alexei M. Orlov

The lipid and fatty acid profile of muscles in beaked redfish, caught and fixed in the wild versus specimens from food supermarkets (“commercial”), were evaluated, as well as the health implications of this popular food for its consumers based on the calculation of nutritional quality indexes. The contents of the total lipids (TLs), total phospholipids (PLs), monoacylglycerols (MAGs), diacylglycerols (DAGs), triacylglycerols (TAGs), cholesterol (Chol), Chol esters, non-esterified fatty acids (NEFAs), and wax esters were determined by HPTLC; the phosphatidylserine (PS), phosphatidylethanolamine (PE), phosphatidylinositol (PI), phosphatidylcholine (PC), and lysophosphatidylcholine (LysoPC) were determined by HPLC; and fatty acids of total lipids were determined using GC. The TL content was higher in commercial products due to DA and NEFAs, among PL fractions the content of LysoPC was also higher. The results indicated multidirectional processes of slight degradation of lipids in commercial products in comparison to wild. The flesh lipid quality index was lower due to EPA and DHA in commercial specimens while the index of thrombogenicity was significantly higher. The differences in the quantities of lipid classes between muscle biopsy regions in fish apparently corroborate the morphology and physiology of deep-water fish.


2016 ◽  
Vol 115 (12) ◽  
pp. 2093-2105 ◽  
Author(s):  
Anne M. Honkanen ◽  
Heidi Leskinen ◽  
Vesa Toivonen ◽  
Nest McKain ◽  
R. John Wallace ◽  
...  

AbstractDescription ofα-linolenic acid (cis-9,cis-12,cis-15-18 : 3, ALA) metabolism in the rumen is incomplete. Ruminal digesta samples were incubated with ALA and buffer containing water or deuterium oxide to investigate the products and mechanisms of ALA biohydrogenation. Geometric Δ9,11,15-18 : 3 isomers were the main intermediates formed from ALA. An increase in then+1 isotopomers of Δ9,11,15-18 : 3 was due to2H labelling at C-13. Isomers of Δ9,11,13-18 : 3,cis-7,cis-12,cis-15-18 : 3 andcis-8,cis-12,cis-15-18 : 3 were also formed. No increase inn+1 isotopomers of Δ7,12,15-18 : 3 or Δ8,12,15-18 : 3 was detected. Enrichment inn+2 isotopomers of 18 : 2 products indicated that ALA metabolism continued via the reduction of 18 : 3 intermediates. Isomers of Δ9,11,15-18 : 3 were reduced to Δ11,15-18 : 2 labelled at C-9 and C-13. ALA resulted in the formation of Δ11,13-18 : 2 and Δ12,14-18 : 2 containing multiple2H labels. Enrichment of then+3 isotopomer of Δ12,15-18 : 2 was also detected. Metabolism of ALA during incubations with rumen contents occurs by one of three distinct pathways. Formation of Δ9,11,15-18 : 3 appears to be initiated by H abstraction on C-13. Octadecatrienoic intermediates containingcis-12 andcis-15 double bonds are formed without an apparent H exchange with water. Labelling of Δ9,11,13-18 : 3 was inconclusive, suggesting formation by an alternative mechanism. These findings explain the appearance of several bioactive fatty acids in muscle and milk that influence the nutritional value of ruminant-derived foods.


Author(s):  
Łuczyńska ◽  
Paszczyk

The objectives of study were to determine heavy metals content (Zn, Cu, Mn, Fe and Hg) and fatty acids in selected organs of roach, Rutilus rutilus (L.); bream, Abramis brama (L.); pike, Esox lucius (L.); Eurasian perch, Perca fluviatilis (L.) collected from reservoirs of Warmia and Mazury region (northeastern Poland). Heavy metals were determined with atomic absorption spectrometry AAS. The fatty acids were analyzed using gas chromatography. In a few cases, differences in the content of heavy metals and fatty acids were not significant between species. The muscles of fish characterized significantly higher values of mercury than other organs (p ≤ 0.05), except for bream. The reverse regularity was observed in the case of content of Cu, Zn, Mn and Fe. Fatty acids having a desirable dietary effect in humans (DFA-Hypocholesterolaemic fatty acids) (74.00–74.84) were more than OFA (hypercholesterolaemic fatty acids), i.e., those undesirable (24.03–24.79). The lipid quality indexes AI (index of atherogenicity) (0.40–0.44) and TI (index of thrombogenicity) (0.18–0.24) in muscles of fish were low, which means that the meat of the fish may be recommended for human health. THQ (target hazard quotient) and HI (hazard index) as individual foodstuff were below 1, whereas HI for a specific receptor/pathway combination exceeded 1. This may suggest that eating meat from a given species is safe from a health point of view.


2011 ◽  
Vol 76 (4) ◽  
pp. C618-C625 ◽  
Author(s):  
Jesús García-Parra ◽  
Francisco González-Cebrino ◽  
Jonathan Delgado ◽  
Mercedes Lozano ◽  
Teresa Hernández ◽  
...  

Author(s):  
Sylwia Jarzynka ◽  
Kamila Strom ◽  
Olga Barbarska ◽  
Emilia Pawlikowska ◽  
Anna Minkiewicz-Zochniak ◽  
...  

Background: Human milk banks have a pivotal role in provide optimal food for those infants who are not fully breastfeed, by allowing human milk from donors to be collected, processed and appropriately distributed. Donor human milk (DHM) is usually preserved by Holder pasteurization, considered to be the gold standard to ensure the microbiology safety and nutritional value of milk. However, as stated by the European Milk Banking Association (EMBA) there is a need to implement the improvement of the operating procedure of human milk banks including preserving and storing techniques. Aim: The purpose of this study was to assess the effectiveness and safety of the selected new combination of methods for preserving donor human milk in comparison with thermal treatment (Holder pasteurization). Methods: We assessed (1) the concentration of bioactive components (insulin, adiponectin, leptin, activity of pancreatic lipase, and hepatocyte growth factor) and (2) microbiological safety in raw and pasteurized, high-pressure processed and lyophilization human breast milk. Results: The combination of two techniques, high-pressure processing and freeze-drying, showed the best potential for preserving the nutritional value of human milk and were evaluated for microbiological safety. Microbiological safety assessment excluded the possibility of using freeze-drying alone for human milk sample preservation. However, it can be used as a method for long-term storage of milk samples, which have previously been preserved via other processes. Conclusion: The results show that high-pressure treatment is the best method for preservation that ensures microbiological safety and biological activity but subsequent freeze-drying allowed long-term storage without loss of properties.


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