scholarly journals Metabolism ofα-linolenic acid during incubations with strained bovine rumen contents: products and mechanisms

2016 ◽  
Vol 115 (12) ◽  
pp. 2093-2105 ◽  
Author(s):  
Anne M. Honkanen ◽  
Heidi Leskinen ◽  
Vesa Toivonen ◽  
Nest McKain ◽  
R. John Wallace ◽  
...  
Keyword(s):  
Fatty Acids ◽  
Linolenic Acid ◽  
Nutritional Value ◽  
Deuterium Oxide ◽  
Alternative Mechanism ◽  
Double Bonds ◽  
Bovine Rumen ◽  
Bioactive Fatty Acids

AbstractDescription ofα-linolenic acid (cis-9,cis-12,cis-15-18 : 3, ALA) metabolism in the rumen is incomplete. Ruminal digesta samples were incubated with ALA and buffer containing water or deuterium oxide to investigate the products and mechanisms of ALA biohydrogenation. Geometric Δ9,11,15-18 : 3 isomers were the main intermediates formed from ALA. An increase in then+1 isotopomers of Δ9,11,15-18 : 3 was due to2H labelling at C-13. Isomers of Δ9,11,13-18 : 3,cis-7,cis-12,cis-15-18 : 3 andcis-8,cis-12,cis-15-18 : 3 were also formed. No increase inn+1 isotopomers of Δ7,12,15-18 : 3 or Δ8,12,15-18 : 3 was detected. Enrichment inn+2 isotopomers of 18 : 2 products indicated that ALA metabolism continued via the reduction of 18 : 3 intermediates. Isomers of Δ9,11,15-18 : 3 were reduced to Δ11,15-18 : 2 labelled at C-9 and C-13. ALA resulted in the formation of Δ11,13-18 : 2 and Δ12,14-18 : 2 containing multiple2H labels. Enrichment of then+3 isotopomer of Δ12,15-18 : 2 was also detected. Metabolism of ALA during incubations with rumen contents occurs by one of three distinct pathways. Formation of Δ9,11,15-18 : 3 appears to be initiated by H abstraction on C-13. Octadecatrienoic intermediates containingcis-12 andcis-15 double bonds are formed without an apparent H exchange with water. Labelling of Δ9,11,13-18 : 3 was inconclusive, suggesting formation by an alternative mechanism. These findings explain the appearance of several bioactive fatty acids in muscle and milk that influence the nutritional value of ruminant-derived foods.

scholarly journals Effect of High-Pressure Processing (HPP) on the Fatty Acid Profile of Different Sized Ragworms (Hediste diversicolor) Cultured in an Integrated Multi-Trophic Aquaculture (IMTA) System

Molecules ◽  
2019 ◽  
Vol 24 (24) ◽  
pp. 4503 ◽  
Author(s):  
Bruna Marques ◽  
Ana Isabel Lillebø ◽  
Maria do Rosário M. Domingues ◽  
Jorge A. Saraiva ◽  
Ricardo Calado
Keyword(s):  
Fatty Acids ◽  
High Pressure ◽  
Circular Economy ◽  
Nutritional Value ◽  
High Pressure Processing ◽  
Double Bonds ◽  
Control Groups ◽  
Hediste Diversicolor ◽  
Lipid Quality ◽  
Quality Indexes

Ragworms (Hediste diversicolor) cultured under integrated multi-trophic aquaculture (IMTA) conditions display an improved fatty acids (FA) profile than conspecifics from the wild, thus being more suitable for maturation diets of marine fish and shrimp. Nonetheless, their use may represent a potential pathway for pathogens. The objective of the present study was to determine if high-pressure processing (HPP), as an approach to safeguard microbiological safety, could promote significant shifts on the FA profiles of different sized ragworms. An analysis of similarities (ANOSIM) revealed the existence of significant differences in the FA profile and lipid quality indexes (atherogenicity (AI), thrombogenicity (TI) and polyene (PI)) of control and HPP treated ragworms of all tested sizes (small, medium and large). Saturated (SFA) and monounsaturated FA (MUFA) increased after HPP, while polyunsaturated FA (PUFA; FA with 2 or 3 double bonds) and highly unsaturated FA (HUFA; FA with ≥ 4 double bonds) decreased. The amount of docosahexaenoic acid (DHA) in polychaetes exposed to HPP decreased an average of 25%, when compared with the levels recorded in control groups. The values of PI significantly decreased after HPP, while those of AI and TI displayed a significant increase. Despite the shifts in the FA profile of ragworms exposed to HPP, these still display a superior profile to that of wild specimens, namely the presence of DHA. Therefore, HPP can be considered as a suitable approach to safeguard the biosecurity of cultured polychaetes, without compromising their nutritional value, and support the principles of circular economy through the use of IMTA.

Effect of wilting and type of additive on the fatty acid composition of grass silage

2002 ◽  
Vol 2002 ◽  
pp. 49-49
Author(s):  
H.E. Warren ◽  
J.K.S. Tweed ◽  
S.J. Youell ◽  
R. J. Dewhurst ◽  
J.D. Wood ◽  
...  
Keyword(s):  
Fatty Acids ◽  
Fatty Acid Composition ◽  
Fatty Acid ◽  
Polyunsaturated Fatty Acids ◽  
Linolenic Acid ◽  
Fatty Acid Profile ◽  
Acid Composition ◽  
Nutritional Value ◽  
Red Clover ◽  
Grass Silage

Forages, such as grass and red clover, are a rich source of n -3 polyunsaturated fatty acids, especially α-linolenic acid (C18:3n -3), and may be used as a method of improving the nutritional value of ruminant products. Silage is an important feed for cattle, therefore, a study was carried out to elucidate the effects of wilting and the use of additives on the fatty acid profile of the resultant silage.

Bioactive fatty acids and cerebrosides from the TCM drug Arisaema sp.

Planta Medica ◽  
2010 ◽  
Vol 76 (12) ◽  
Author(s):  
E Rozema ◽  
N Fakhrudin ◽  
A Atanasov ◽  
D Schuster ◽  
E Heiss ◽  
...  
Keyword(s):  
Fatty Acids ◽  
Bioactive Fatty Acids

scholarly journals Explore the gene network regulating the composition of fatty acids in cottonseed

2021 ◽  
Vol 21 (1) ◽  
Author(s):  
Lihong Ma ◽  
Xinqi Cheng ◽  
Chuan Wang ◽  
Xinyu Zhang ◽  
Fei Xue ◽  
...  
Keyword(s):  
Fatty Acids ◽  
Fatty Acid ◽  
Linoleic Acid ◽  
Linolenic Acid ◽  
Gene Network ◽  
Unsaturated Fatty Acids ◽  
Saturated Fatty Acids ◽  
Accumulation Pattern ◽  
Time Points ◽  
Expression Characteristics

Abstract Background Cottonseed is one of the major sources of vegetable oil. Analysis of the dynamic changes of fatty acid components and the genes regulating the composition of fatty acids of cottonseed oil is of great significance for understanding the biological processes underlying biosynthesis of fatty acids and for genetic improving the oil nutritional qualities. Results In this study, we investigated the dynamic relationship of 13 fatty acid components at 12 developmental time points of cottonseed (Gossypium hirsutum L.) and generated cottonseed transcriptome of the 12 time points. At 5–15 day post anthesis (DPA), the contents of polyunsaturated linolenic acid (C18:3n-3) and saturated stearic acid (C18:0) were higher, while linoleic acid (C18:2n-6) was mainly synthesized after 15 DPA. Using 5 DPA as a reference, 15,647 non-redundant differentially expressed genes were identified in 10–60 DPA cottonseed. Co-expression gene network analysis identified six modules containing 3275 genes significantly associated with middle-late seed developmental stages and enriched with genes related to the linoleic acid metabolic pathway and α-linolenic acid metabolism. Genes (Gh_D03G0588 and Gh_A02G1788) encoding stearoyl-ACP desaturase were identified as hub genes and significantly up-regulated at 25 DPA. They seemed to play a decisive role in determining the ratio of saturated fatty acids to unsaturated fatty acids. FAD2 genes (Gh_A13G1850 and Gh_D13G2238) were highly expressed at 25–50 DPA, eventually leading to the high content of C18:2n-6 in cottonseed. The content of C18:3n-3 was significantly decreased from 5 DPA (7.44%) to 25 DPA (0.11%) and correlated with the expression characteristics of Gh_A09G0848 and Gh_D09G0870. Conclusions These results contribute to our understanding on the relationship between the accumulation pattern of fatty acid components and the expression characteristics of key genes involved in fatty acid biosynthesis during the entire period of cottonseed development.

scholarly journals Polyunsaturated ω-3 fatty acids inhibit ACE2-controlled SARS-CoV-2 binding and cellular entry

2021 ◽  
Vol 11 (1) ◽  
Author(s):  
Anna Goc ◽  
Aleksandra Niedzwiecki ◽  
Matthias Rath
Keyword(s):  
Fatty Acids ◽  
Polyunsaturated Fatty Acids ◽  
Eicosapentaenoic Acid ◽  
Linolenic Acid ◽  
Cathepsin L ◽  
Saturated Fatty Acids ◽  
Monounsaturated Fatty Acids ◽  
In Vivo Experiments ◽  
Lipid Compounds

AbstractThe strain SARS-CoV-2, newly emerged in late 2019, has been identified as the cause of COVID-19 and the pandemic declared by WHO in early 2020. Although lipids have been shown to possess antiviral efficacy, little is currently known about lipid compounds with anti-SARS-CoV-2 binding and entry properties. To address this issue, we screened, overall, 17 polyunsaturated fatty acids, monounsaturated fatty acids and saturated fatty acids, as wells as lipid-soluble vitamins. In performing target-based ligand screening utilizing the RBD-SARS-CoV-2 sequence, we observed that polyunsaturated fatty acids most effectively interfere with binding to hACE2, the receptor for SARS-CoV-2. Using a spike protein pseudo-virus, we also found that linolenic acid and eicosapentaenoic acid significantly block the entry of SARS-CoV-2. In addition, eicosapentaenoic acid showed higher efficacy than linolenic acid in reducing activity of TMPRSS2 and cathepsin L proteases, but neither of the fatty acids affected their expression at the protein level. Also, neither reduction of hACE2 activity nor binding to the hACE2 receptor upon treatment with these two fatty acids was observed. Although further in vivo experiments are warranted to validate the current findings, our study provides a new insight into the role of lipids as antiviral compounds against the SARS-CoV-2 strain.

scholarly journals Prepubertal Dietary and Plasma Phospholipid Fatty Acids Related to Puberty Timing: Longitudinal Cohort and Mendelian Randomization Analyses

Nutrients ◽  
2021 ◽  
Vol 13 (6) ◽  
pp. 1868
Author(s):  
Tuck Seng Cheng ◽  
Felix R. Day ◽  
John R. B. Perry ◽  
Jian’an Luan ◽  
Claudia Langenberg ◽  
...  
Keyword(s):  
Fatty Acids ◽  
Linolenic Acid ◽  
Mendelian Randomization ◽  
Saturated Fatty Acids ◽  
Plasma Phospholipid ◽  
Height Velocity ◽  
Breast Development ◽  
Dietary Intakes ◽  
Linear Regression Models ◽  
Peak Height Velocity

Dietary intakes of polyunsaturated, monounsaturated and saturated fatty acids (FAs) have been inconsistently associated with puberty timing. We examined longitudinal associations of prepubertal dietary and plasma phospholipid FAs with several puberty timing traits in boys and girls. In the Avon Longitudinal Study of Parents and Children, prepubertal fat intakes at 3–7.5 years and plasma phospholipid FAs at 7.5 years were measured. Timings of Tanner stage 2 genital or breast development and voice breaking or menarche from repeated reports at 8–17 years, and age at peak height velocity (PHV) from repeated height measurements at 5–20 years were estimated. In linear regression models with adjustment for maternal and infant characteristics, dietary substitution of polyunsaturated FAs for saturated FAs, and higher concentrations of dihomo-γ-linolenic acid (20:3n6) and palmitoleic acid (16:1n7) were associated with earlier timing of puberty traits in girls (n = 3872) but not boys (n = 3654). In Mendelian Randomization models, higher genetically predicted circulating dihomo-γ-linolenic acid was associated with earlier menarche in girls. Based on repeated dietary intake data, objectively measured FAs and genetic causal inference, these findings suggest that dietary and endogenous metabolic pathways that increase plasma dihomo-γ-linolenic acid, an intermediate metabolite of n-6 polyunsaturated FAs, may promote earlier puberty timing in girls.

The effect of supplemental bioactive fatty acids on growth performance and immune function of milk-fed Holstein dairy calves during heat stress

2021 ◽  
pp. 1-38
Author(s):  
B. Mohtashami ◽  
H. Khalilvandi-Behroozyar ◽  
R. Pirmohammadi ◽  
M. Dehghan-Banadaky ◽  
M. Kazemi-Bonchenari ◽  
...  
Keyword(s):  
Fatty Acids ◽  
Immune Function ◽  
Growth Performance ◽  
Average Daily Gain ◽  
Dairy Calves ◽  
Necrosis Factor Alpha ◽  
Amyloid A ◽  
Bioactive Fatty Acids ◽  
Hot Season ◽  
Experimental Treatments

Abstract This study aimed to evaluate the effects of different supplemental fat sources [soybean oil (SBO) as a source of n-6 fatty acid and fish oil (FO) as a source of n-3 fatty acids] in the starter feed of milk-fed dairy calves during the hot season. Forty Holstein calves (3 d of age; 39.67 kg of body weight; ten calves per group) were randomly assigned to the experimental treatments as follows: (1) starter feed supplemented with no fat source (CON), (2) starter feed supplemented with 3% SBO (DM basis), (3) starter feed supplemented with 3% FO (DM basis), and (4) starter feed supplemented with an equal mixture of SBO and FO (1.5% each, DM basis). The milk feeding schedule was constant for treatments and all calves were weaned on d 65 of age. Results show that calves had greater starter intake, average daily gain, and body length when fed SBO compared to other treatments. However, feed efficiency was increased and inflammatory indicators (tumor necrosis factor-alpha, serum amyloid A and haptoglobin) concentrations were reduced in the calves fed FO compared to the other treatments. In summary, it was revealed that SBO rich in n-6 FA improved starter intake and growth performance, while FO rich in n-3 FA could improve the immune function of calves. Due to the current experimental condition, an equal mixture of SBO and FO (1.5% each, DM basis) can be recommended to have an optimum growth performance and immune function while the calves are reared under the heat conditions.

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