Comparative Analysis of Lipids and Fatty Acids in Beaked Redfish Sebastes mentella Travin, 1951 Collected in Wild and in Commercial Products

The lipid and fatty acid profile of muscles in beaked redfish, caught and fixed in the wild versus specimens from food supermarkets (“commercial”), were evaluated, as well as the health implications of this popular food for its consumers based on the calculation of nutritional quality indexes. The contents of the total lipids (TLs), total phospholipids (PLs), monoacylglycerols (MAGs), diacylglycerols (DAGs), triacylglycerols (TAGs), cholesterol (Chol), Chol esters, non-esterified fatty acids (NEFAs), and wax esters were determined by HPTLC; the phosphatidylserine (PS), phosphatidylethanolamine (PE), phosphatidylinositol (PI), phosphatidylcholine (PC), and lysophosphatidylcholine (LysoPC) were determined by HPLC; and fatty acids of total lipids were determined using GC. The TL content was higher in commercial products due to DA and NEFAs, among PL fractions the content of LysoPC was also higher. The results indicated multidirectional processes of slight degradation of lipids in commercial products in comparison to wild. The flesh lipid quality index was lower due to EPA and DHA in commercial specimens while the index of thrombogenicity was significantly higher. The differences in the quantities of lipid classes between muscle biopsy regions in fish apparently corroborate the morphology and physiology of deep-water fish.

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Estimation of Lipid Fractions of Blood Plasma

Clinical Chemistry ◽

10.1093/clinchem/1.5.317 ◽

1955 ◽

Vol 1 (5) ◽

pp. 317-323 ◽

Cited By ~ 1

Author(s):

Andre C Kibrick ◽

S J Skupp

Keyword(s):

Fatty Acids ◽

Oil Recovery ◽

Volumetric Method ◽

Total Lipids ◽

Average Value ◽

Esterified Fatty Acids ◽

Normal Individuals ◽

Lipid Fractions ◽

Total Fatty Acids ◽

Entire Procedure

Abstract The Allen volumetric method for plasma fat has been modified by calibration to include the percentage of olive-oil recovery. The values obtained from calibration with mercury are corrected for the 125 per cent recovery of the oil. The validity of the entire procedure has been discussed. A simple system of estimating the lipid fractions of plasma has been described. Total lipids are the total of Allen fat + (25 x lipid P). Neutral fat is Allen fat-cholesterol-cholesterol fatty acids. Total fatty acids are X neutral fat + cholesterol fatty acids + (0.64 x phospholipids). The values of total fatty acids have been compared with those obtained by oxidation with dichromate and by determining the esterified fatty acids with iron perchlorate. The values of neutral fat found in 13 apparently normal individuals are shown to range from 0 to 88 and to give an average value of 28 mg./100 ml.

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Effect of High-Pressure Processing (HPP) on the Fatty Acid Profile of Different Sized Ragworms (Hediste diversicolor) Cultured in an Integrated Multi-Trophic Aquaculture (IMTA) System

Molecules ◽

10.3390/molecules24244503 ◽

2019 ◽

Vol 24 (24) ◽

pp. 4503 ◽

Cited By ~ 1

Author(s):

Bruna Marques ◽

Ana Isabel Lillebø ◽

Maria do Rosário M. Domingues ◽

Jorge A. Saraiva ◽

Ricardo Calado

Keyword(s):

Fatty Acids ◽

High Pressure ◽

Circular Economy ◽

Nutritional Value ◽

High Pressure Processing ◽

Double Bonds ◽

Control Groups ◽

Hediste Diversicolor ◽

Lipid Quality ◽

Quality Indexes

Ragworms (Hediste diversicolor) cultured under integrated multi-trophic aquaculture (IMTA) conditions display an improved fatty acids (FA) profile than conspecifics from the wild, thus being more suitable for maturation diets of marine fish and shrimp. Nonetheless, their use may represent a potential pathway for pathogens. The objective of the present study was to determine if high-pressure processing (HPP), as an approach to safeguard microbiological safety, could promote significant shifts on the FA profiles of different sized ragworms. An analysis of similarities (ANOSIM) revealed the existence of significant differences in the FA profile and lipid quality indexes (atherogenicity (AI), thrombogenicity (TI) and polyene (PI)) of control and HPP treated ragworms of all tested sizes (small, medium and large). Saturated (SFA) and monounsaturated FA (MUFA) increased after HPP, while polyunsaturated FA (PUFA; FA with 2 or 3 double bonds) and highly unsaturated FA (HUFA; FA with ≥ 4 double bonds) decreased. The amount of docosahexaenoic acid (DHA) in polychaetes exposed to HPP decreased an average of 25%, when compared with the levels recorded in control groups. The values of PI significantly decreased after HPP, while those of AI and TI displayed a significant increase. Despite the shifts in the FA profile of ragworms exposed to HPP, these still display a superior profile to that of wild specimens, namely the presence of DHA. Therefore, HPP can be considered as a suitable approach to safeguard the biosecurity of cultured polychaetes, without compromising their nutritional value, and support the principles of circular economy through the use of IMTA.

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Mercury, Fatty Acids Content and Lipid Quality Indexes in Muscles of Freshwater and Marine Fish on the Polish Market. Risk Assessment of Fish Consumption

International Journal of Environmental Research and Public Health ◽

10.3390/ijerph14101120 ◽

2017 ◽

Vol 14 (10) ◽

pp. 1120 ◽

Cited By ~ 14

Author(s):

Joanna Łuczyńska ◽

Beata Paszczyk ◽

Joanna Nowosad ◽

Marek Łuczyński

Keyword(s):

Risk Assessment ◽

Fatty Acids ◽

Marine Fish ◽

Fish Consumption ◽

Market Risk ◽

Lipid Quality ◽

Quality Indexes

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The absorption of long-chain fatty acids from the small intestine of the sheep

British Journal Of Nutrition ◽

10.1079/bjn19680030 ◽

1968 ◽

Vol 22 (2) ◽

pp. 247-254 ◽

Cited By ~ 25

Author(s):

Aileen M. Lennox ◽

G. A. Garton

Keyword(s):

Fatty Acids ◽

Small Intestine ◽

Neutral Lipids ◽

Long Chain Fatty Acids ◽

Linseed Meal ◽

Total Lipids ◽

Esterified Fatty Acids ◽

Total Fatty Acids ◽

Long Chain ◽

Different Parts

1. Three sheep, each of which was fitted with a rumen cannula and with two pairs of reentrant cannulas in different parts of the small intestine, were used in this study. They were fed on dried grass cubes or hay plus linseed meal and oats: an aqueous solution of polyethylene glycol (PEG) was infused continuously into the rumen.2. Total lipids were extracted from samples of the chyme entering and leaving the different lengths of the small intestine embraced by the respective cannulas. The lipids were fractionated into unesterified fatty acids, neutral lipids and phospholipids and the contribution of each fraction to the total fatty acids was determined. The samples were also analysed for their PEG content, thus affording an index of the extent to which water had been absorbed from each particular length of intestine.3. From the above findings and a knowledge of the flow-rate of the digesta, the uptake of unesterified fatty acids and the degree of dissimilation or uptake, or of both, of esterified fatty acids was calculated.4. The results indicated that, by the time the digesta reached the ileum (i.e. the distal half of the small intestine), the uptake of fatty acids was almost complete, as was also the hydrolytic release of esterified fatty acids.5. Though there were no gross differences in the overall composition of the unesterified and esterified fatty acids in different parts of the small intestine, it appeared that C18 mono-unsaturated acid, the principal unsaturated unesterified acid, was absorbed somewhat more efficiently than were the major saturated acids (palmitic acid and stearic acid).

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Health Risk Assessment of Heavy Metals and Lipid Quality Indexes in Freshwater Fish from Lakes of Warmia and Mazury Region, Poland

International Journal of Environmental Research and Public Health ◽

10.3390/ijerph16193780 ◽

2019 ◽

Vol 16 (19) ◽

pp. 3780 ◽

Cited By ~ 4

Author(s):

Łuczyńska ◽

Paszczyk

Keyword(s):

Heavy Metals ◽

Fatty Acids ◽

Hazard Quotient ◽

Hazard Index ◽

Perca Fluviatilis ◽

Absorption Spectrometry ◽

Target Hazard Quotient ◽

Roach Rutilus Rutilus ◽

Lipid Quality ◽

Quality Indexes

The objectives of study were to determine heavy metals content (Zn, Cu, Mn, Fe and Hg) and fatty acids in selected organs of roach, Rutilus rutilus (L.); bream, Abramis brama (L.); pike, Esox lucius (L.); Eurasian perch, Perca fluviatilis (L.) collected from reservoirs of Warmia and Mazury region (northeastern Poland). Heavy metals were determined with atomic absorption spectrometry AAS. The fatty acids were analyzed using gas chromatography. In a few cases, differences in the content of heavy metals and fatty acids were not significant between species. The muscles of fish characterized significantly higher values of mercury than other organs (p ≤ 0.05), except for bream. The reverse regularity was observed in the case of content of Cu, Zn, Mn and Fe. Fatty acids having a desirable dietary effect in humans (DFA-Hypocholesterolaemic fatty acids) (74.00–74.84) were more than OFA (hypercholesterolaemic fatty acids), i.e., those undesirable (24.03–24.79). The lipid quality indexes AI (index of atherogenicity) (0.40–0.44) and TI (index of thrombogenicity) (0.18–0.24) in muscles of fish were low, which means that the meat of the fish may be recommended for human health. THQ (target hazard quotient) and HI (hazard index) as individual foodstuff were below 1, whereas HI for a specific receptor/pathway combination exceeded 1. This may suggest that eating meat from a given species is safe from a health point of view.

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Into the Deep: New Data on the Lipid and Fatty Acid Profile of Redfish Sebastes mentella Inhabiting Different Depths in the Irminger Sea

Biomolecules ◽

10.3390/biom11050704 ◽

2021 ◽

Vol 11 (5) ◽

pp. 704

Author(s):

Viktor P. Voronin ◽

Nina N. Nemova ◽

Tatjana R. Ruokolainen ◽

Dmitrii V. Artemenkov ◽

Aleksei Y. Rolskii ◽

...

Keyword(s):

Fatty Acids ◽

Fatty Acid ◽

Wax Ester ◽

Total Lipids ◽

Irminger Sea ◽

Physicochemical State ◽

Different Depths ◽

Males And Females ◽

Species Specific ◽

Sebastes Mentella

New data on lipid and fatty acid profiles are presented, and the dynamics of the studied components in muscles in the males and females of the beaked redfish, Sebastes mentella, in the depth gradient of the Irminger Sea (North Atlantic) is discussed. The contents of the total lipids (TLs), total phospholipids (PLs), monoacylglycerols (MAGs), diacylglycerols (DAGs), triacylglycerols (TAGs), cholesterol (Chol), Chol esters, non-esterified fatty acids (NEFAs), and wax esters were determined by HPTLC; the phosphatidylserine (PS), phosphatidylethanolamine (PE), phosphatidylinositol (PI), phosphatidylcholine (PC), and lysophosphatidylcholine (LPC) were determined by HPLC; and fatty acids of total lipids were determined using GC. The Chol esters prevailed in muscles over the storage TAGs, and the wax ester content was high, which is a characteristic trait of vertically migrating species. Specific dynamics in certain PL in redfish were found to be depended on depth, suggesting that PLs are involved in the re-arrangement of the membrane physicochemical state and the maintenance of motor activity under high hydrostatic pressure. The high contents of DHA and EPA were observed in beaked redfish muscles is the species’ characteristic trait. The MUFAs in muscles include dietary markers of zooplankton (copepods)—20:1(n-9) and 22:1(n-11), whose content was found to be lower in fish sampled from greater depths.

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Quantification of Essential Fatty Acids and Assessment of the Nutritional Quality Indexes of Lipids in Tilapia Alevins and Juvenile Tilapia Fish (Oreochromis niloticus)

Journal of Food Research ◽

10.5539/jfr.v3n3p105 ◽

2014 ◽

Vol 3 (3) ◽

pp. 105 ◽

Cited By ~ 12

Author(s):

Ivane Benedetti Tonial ◽

Debora Francielly Oliveira ◽

Alexandre Rodrigo Coelho ◽

Makoto Matsushita ◽

Fabio Augusto Garcia Coró ◽

...

Keyword(s):

Fatty Acids ◽

Nutritional Quality ◽

Essential Fatty Acids ◽

Juvenile Fish ◽

Atherogenic Index ◽

Total Lipids ◽

Beneficial Effects ◽

Thrombogenic Index ◽

Quality Indexes

<p>To determine the nutritional quality of the lipid segment in tilapia (<em>Orechromis niloticus</em>) during different periods of development (alevins and juveniles), the total lipids (TL), linolenic (LNA), eicosapentaenoic (EPA), docosapentaenoic (DHA), linoleic (LA) and arachidonic (AA) acids were quantified , and the lipid nutritional quality indexes were calculated for the tilapia. The lipid profile showed that the species present high indexes of monounsaturated (MUFA) and polyunsaturated fatty acids (PUFA) in both development phases. The indexes of nutritional quality of lipids, atherogenic index (AI) and thrombogenic index (TI) present low values and represent beneficial health effects, both in the alevin and juvenile fish. The quantifications of LNA, EPA, DHA, LA and AA show the greatest values (mg/g of total lipids) for the juveniles. However, regardless of the development phase in which the tilapia is, the fish may be considered a source of essential fatty acids a kind of potential and nutritional food, reflecting beneficial effects for consumer’s health.</p>

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