scholarly journals Effectiveness of Different Analytical Methods for the Characterization of Propolis: A Case of Study in Northern Italy

Molecules ◽  
2020 ◽  
Vol 25 (3) ◽  
pp. 504 ◽  
Author(s):  
Radmila Pavlovic ◽  
Gigliola Borgonovo ◽  
Valeria Leoni ◽  
Luca Giupponi ◽  
Giulia Ceciliani ◽  
...  
Keyword(s):  
Analytical Methods ◽  
Radical Scavenging Activity ◽  
Folk Medicine ◽  
Radical Scavenging ◽  
Analytical Techniques ◽  
Northern Italy ◽  
Spectrophotometric Analysis ◽  
Po Valley ◽  
Mountain Environments ◽  
Pharmaceutical Properties

Propolis is used as folk medicine due to its spectrum of alleged biological and pharmaceutical properties and it is a complex matrix not still totally characterized. Two batches of propolis coming from two different environments (plains of Po Valley and the hilly Ligurian–Piedmont Apennines) of Northern Italy were characterized using different analytical methods: Spectrophotometric analysis of phenols, flavones and flavonols, and DPPH radical scavenging activity, HPLC, NMR, HSPME and GC–MS and HPLC–MS Orbitrap. Balsam and moisture content were also considered. No statistical differences were found at the spectrophotometric analysis; balsam content did not vary significantly. The most interesting findings were in the VOCs composition, with the Po Valley samples containing compounds of the resins from leaf buds of Populus nigra L. The hills (Appennines) samples were indeed characterize by the presence of phenolic glycerides already found in mountain environments. HPLC–Q-Exactive-Orbitrap®–MS analysis is crucial in appropriate recognition of evaluate number of metabolites, but also NMR itself could give more detailed information especially when isomeric compounds should be identified. It is necessary a standardized evaluation to protect and valorize this production and more research on propolis characterization using different analytical techniques.

scholarly journals The Health-Promoting Potential of Salix spp. Bark Polar Extracts: Key Insights on Phenolic Composition and In Vitro Bioactivity and Biocompatibility

Antioxidants ◽  
2019 ◽  
Vol 8 (12) ◽  
pp. 609 ◽  
Author(s):  
Patrícia A. B. Ramos ◽  
Catarina Moreirinha ◽  
Sara Silva ◽  
Eduardo M. Costa ◽  
Mariana Veiga ◽  
...  
Keyword(s):  
High Performance ◽  
Radical Scavenging Activity ◽  
Folk Medicine ◽  
Radical Scavenging ◽  
Mass Spectrometry Analysis ◽  
Spectrometry Analysis ◽  
Phenolic Components ◽  
Polar Extracts ◽  
Salix Spp

Salix spp. have been exploited for energy generation, along with folk medicine use of bark extracts for antipyretic and analgesic benefits. Bark phenolic components, rather than salicin, have demonstrated interesting bioactivities, which may ensure the sustainable bioprospection of Salix bark. Therefore, this study highlights the detailed phenolic characterization, as well as the in vitro antioxidant, anti-hypertensive, Staphylococcus aureus growth inhibitory effects, and biocompatibility of Salix atrocinerea Brot., Salix fragilis L., and Salix viminalis L. bark polar extracts. Fifteen phenolic compounds were characterized by ultra-high-performance liquid chromatography-ultraviolet detection-mass spectrometry analysis, from which two flavan-3-ols, an acetophenone, five flavanones, and a flavonol were detected, for the first time, as their bark components. Salix bark extracts demonstrated strong free radical scavenging activity (5.58–23.62 µg mL−1 IC50 range), effective inhibition on angiotensin-I converting enzyme (58–84%), and S. aureus bactericidal action at 1250–2500 µg mL−1 (6–8 log CFU mL−1 reduction range). All tested Salix bark extracts did not show cytotoxic potential against Caco-2 cells, as well as S. atrocinerea Brot. and S. fragilis L. extracts at 625 and 1250 µg mL−1 against HaCaT and L929 cells. These valuable findings can pave innovative and safer food, nutraceutical, and/or cosmetic applications of Salix bark phenolic-containing fractions.

scholarly journals Muscari comosum L. Bulb Extracts Modulate Oxidative Stress and Redox Signaling in HepG2 Cells

Molecules ◽  
2021 ◽  
Vol 26 (2) ◽  
pp. 416
Author(s):  
Fabiana Giglio ◽  
Maria Antonietta Castiglione Morelli ◽  
Ilenia Matera ◽  
Chiara Sinisgalli ◽  
Rocco Rossano ◽  
...  
Keyword(s):  
Antioxidant Activity ◽  
Hepg2 Cells ◽  
Biological Activities ◽  
Radical Scavenging Activity ◽  
Redox Homeostasis ◽  
Folk Medicine ◽  
Radical Scavenging ◽  
In Vitro Assays ◽  
South Italy

Muscari comosum L. bulbs are commonly used as food in South Italy and also in folk medicine. By evaluating in vitro antioxidant activity and biological activities of their aqueous and methanol extracts, we shed light on the potential role, including both the nutraceutical and health benefits, of this plant. Total polyphenol content (TPC) and total flavonoid content (TFC) were evaluated by the Folin–Ciocalteu method and by the aluminum chloride method, respectively. Antioxidant activity was investigated by three in vitro assays and relative antioxidant capacity index (RACI) was calculated to compare results obtained by different tests. The extracts were tested to evaluate their possible involvement in redox homeostasis, using the human hepatoma (HepG2) cell line used as model. The extracts exhibited concentration/solvent dependent radical scavenging activity, as well as dysregulation of some genes involved in redox pathways by promoting Nrf2, SOD-2, GPX1, ABCC6 and ABCG2 expression. NMR metabolomics analysis suggests that HepG2 cells treated with Muscari comosum extracts experience changes in some metabolites involved in various metabolic pathways.

scholarly journals Anti-inflammatory and antinociceptive features of Bryonia alba L.: As a possible alternative in treating rheumatism

2019 ◽  
Vol 17 (1) ◽  
pp. 23-30 ◽  
Author(s):  
Mert Ilhan ◽  
Fatma Tuğçe Gürağaç Dereli ◽  
Ibrahim Tümen ◽  
Esra Küpeli Akkol
Keyword(s):  
Antioxidant Activities ◽  
Radical Scavenging Activity ◽  
Folk Medicine ◽  
Radical Scavenging ◽  
Total Phenolic ◽  
Tail Flick ◽  
Scavenging Activity ◽  
Tail Flick Test ◽  
Etoac Extract ◽  
Anti Inflammatory

AbstractBryonia species have traditionally been used as a diuretic and laxative, to reduce edema and relieve joint pain. B. alba roots are used especially to treat rheumatic pain and applied to painful joints in Turkish folk medicine. B. alba roots were extracted with n-hexane, ethyl acetate (EtOAc), and methanol, successively. The carrageenan-induced hind paw edema model and the Whittle method were used to evaluate the anti-inflammatory activity of the extracts in mice. The p-benzoquinone-induced abdominal constriction test and tail flick test were used to evaluate the antinociceptive activity of the extracts in mice. DPPH-radical-scavenging activity, ABTS radical–scavenging activity, total antioxidant activity, and hydroxyl radical– scavenging activity were assessed. Furthermore, the total phenolic and total flavonoid contents were calculated for all the extracts. The EtOAc extract prepared from B. alba roots had the most promising anti-inflammatory, antinociceptive, and antioxidant activities. Moreover, it had high phenolic and flavonoid contents. Therefore, B. alba roots could be alternative treatment method for rheumatic disorders.

scholarly journals Discovery of Some Emerging Free Radical Scavenging Candidates Bearing 2-(P-hydroxyphenyl)-4–(Substitutedphenyl)-1H-1,5–Benzodiazepines Pharmacophore

2017 ◽  
Vol 9 (04) ◽  
Author(s):  
Animeshchandra G. M. Haldar ◽  
Santosh S. Chhajed ◽  
Debarshi Kar Mahapatra ◽  
Debarshi Kar Mahapatra
Keyword(s):  
Antioxidant Activity ◽  
Free Radical ◽  
Radical Scavenging Activity ◽  
Radical Scavenging ◽  
Analytical Techniques ◽  
Free Radical Scavenging ◽  
Free Radical Scavengers ◽  
Scavenging Activity ◽  
In Vitro Antioxidant Activity

In the present investigation, the synthesis of few novel leads bearing 2-(p-hydroxyphenyl)-4–(substitutedphenyl)-1H-1,5–benzodiazepine pharmacophore is described. The substituted chalcone and their derivatives 3(a-j) were synthesized by base catalyzed Claisen-Schmidt condensation between p-hydroxy-acetophenone and appropriate aldehydes. The dibromostyryl ketones 4(a-j) were obtained by the reaction the chalcone with bromine in acetic acid. The dibromostyryl ketone were reacted with methanol in presence of sodium methoxide followed by acidic hydrolysis give 1-(4-hydroxyphenyl)-3-(substitutedphenyl)-1,3-propanediones. The targeted compounds; the substituted 1,5-benzodiazepines were synthesized with o-phenylenediamine and synthesized 1,3-propanediones. The structures of synthesized compounds were confirmed by spectroscopic and analytical techniques (IR, 1H-NMR, and MS). The free radical scavenging activity of the synthesized analogs was monitored by in vitro antioxidant activity protocol. The derivatives 6f, 6g, 6i, and 6j were found to exhibit good antioxidant activity with 59.07%, 41.33%, 68.3% and 60.4% scavenging activity respectively as compared to standard ascorbic acid which demonstrated 79.73% activity. The current research revealed the potential of 2-(p-hydroxyphenyl)-4–(substituted-phenyl)-1H-1,5–benzodiazepine as emerging free radical scavengers. The study helped to establish a structure-activity relationship (SAR) where the substitution on the phenyl moiety of the 1,5-benzodiazepine was found to play profound role and influence over biological activity. The research will open new avenues for the development of antioxidant moieties having perspectives in cancer, inflammation, and several other ailments.

FREE RADICAL SCAVENGING ACTIVITY OF ACACIA NILOTICA PODS

INDIAN DRUGS ◽  
2014 ◽  
Vol 51 (07) ◽  
pp. 14-22
Author(s):  
T. A Khan ◽  
◽  
A. Gohel
Keyword(s):  
Lipid Peroxidation ◽  
Free Radical ◽  
Radical Scavenging Activity ◽  
Folk Medicine ◽  
Radical Scavenging ◽  
Acacia Nilotica ◽  
Free Radical Scavengers ◽  
Radical Scavengers ◽  
Radical Production ◽  
Medicinal Value

Biomolecules can be oxidized by free radicals. This oxidative damage has an important etiological role in aging and the development of diseases like cancer, atherosclerosis, and other inflammatory disorders. Synthetic antioxidants, such as butylated hydroxyl anisole, are good free radical scavengers; however, they can be carcinogenic. Therefore, there is an increasing interest in searching for antioxidants of natural origin. Acacia nilotica has a versatile medicinal value as folk medicine. The present study aims at screening the methanol and aqueous extracts of the pods of Acacia nilotica for antioxidant activity. The effect of these extracts on inhibition of 1, 1-diphenyl-2-picrylhydrazyl (DPPH), nitric oxide radicals and of lipid peroxidation was studied. The results indicated that both the extracts exhibited a significant reduction in the DPPH and NO radical production along with a significant reduction in lipid peroxidation. The results indicate the pods of Acacia nilotica to be a cheap and easily available source of antioxidants

scholarly journals Identification and Quantitation of Flavanols and Proanthocyanidins in Foods: How Good are the Datas?

2005 ◽  
Vol 12 (1) ◽  
pp. 35-41 ◽  
Author(s):  
Mark A. Kelm ◽  
John F. Hammerstone ◽  
Harold H. Schmitz
Keyword(s):  
High Performance ◽  
Cardiovascular Health ◽  
Radical Scavenging Activity ◽  
Radical Scavenging ◽  
Analytical Techniques ◽  
Food Composition ◽  
Standard Material ◽  
Dietary Polyphenols ◽  
Limited Food ◽  
Food Composition Data

Evidence suggesting that dietary polyphenols, flavanols, and proanthocyanidins in particular offer significant cardiovascular health benefits is rapidly increasing. Accordingly, reliable and accurate methods are needed to provide qualitative and quantitative food composition data necessary for high quality epidemiological and clinical research. Measurements for flavonoids and proanthocyanidins have employed a range of analytical techniques, with various colorimetric assays still being popular for estimating total polyphenolic content in foods and other biological samples despite advances made with more sophisticated analyses. More crudely, estimations of polyphenol content as well as antioxidant activity are also reported with values relating to radical scavenging activity. High-performance liquid chromatography (HPLC) is the method of choice for quantitative analysis of individual polyphenols such as flavanols and proanthocyanidins. Qualitative information regarding proanthocyanidin structure has been determined by chemical methods such as thiolysis and by HPLC-mass spectrometry (MS) techniques at present. The lack of appropriate standards is the single most important factor that limits the aforementioned analyses. However, with ever expanding research in the arena of flavanols, proanthocyanidins, and health and the importance of their future inclusion in food composition databases, the need for standards becomes more critical. At present, sufficiently well-characterized standard material is available for selective flavanols and proanthocyanidins, and construction of at least a limited food composition database is feasible.

scholarly journals Chemical Characterization of Fruit Wine Made from Oblačinska Sour Cherry

2014 ◽  
Vol 2014 ◽  
pp. 1-9 ◽  
Author(s):  
Milica Pantelić ◽  
Dragana Dabić ◽  
Saša Matijašević ◽  
Sonja Davidović ◽  
Biljana Dojčinović ◽  
...  
Keyword(s):  
Radical Scavenging Activity ◽  
Radical Scavenging ◽  
Chemical Characterization ◽  
Sour Cherry ◽  
Analytical Techniques ◽  
Anthocyanin Content ◽  
Total Anthocyanin ◽  
Comprehensive Information ◽  
Total Anthocyanin Content

This paper was aimed at characterizing the wine obtained from Oblačinska, a native sour cherry cultivar. To the best of our knowledge, this is the first paper with the most comprehensive information on chemical characterization of Oblačinska sour cherry wine. The chemical composition was characterized by hyphenated chromatographic methods and traditional analytical techniques. A total of 24 compounds were quantified using the available standards and another 22 phenolic compounds were identified based on the accurate mass spectrographic search. Values of total phenolics content, total anthocyanin content, and radical scavenging activity for cherry wine sample were 1.938 mg gallic acid eqv L−1, 0.113 mg cyanidin-3-glucoside L−1, and 34.56%, respectively. In general, cherry wine polyphenolics in terms of nonanthocyanins and anthocyanins were shown to be distinctive when compared to grape wines. Naringenin and apigenin were characteristic only for cherry wine, and seven anthocyanins were distinctive for cherry wine.

scholarly journals Glochidion littorale Leaf Extract Exhibits Neuroprotective Effects in Caenorhabditis elegans via DAF-16 Activation

Molecules ◽  
2021 ◽  
Vol 26 (13) ◽  
pp. 3958
Author(s):  
Abdel Fawaz Bagoudou ◽  
Yifeng Zheng ◽  
Masahiro Nakabayashi ◽  
Saroat Rawdkuen ◽  
Hyun-Young Park ◽  
...  
Keyword(s):  
Caenorhabditis Elegans ◽  
Leaf Extract ◽  
Radical Scavenging Activity ◽  
Folk Medicine ◽  
Radical Scavenging ◽  
Neuroprotective Activity ◽  
The Novel ◽  
Leaf Extracts ◽  
Neuroprotective Effects

A number of plants used in folk medicine in Thailand and Eastern Asia are attracting interest due to the high bioactivities of their extracts. The aim of this study was to screen the edible leaf extracts of 20 plants found in Thailand and investigate the potential neuroprotective effects of the most bioactive sample. The total phenol and flavonoid content and 2,2-diphenyl-1-picrylhydrazyl radical-scavenging activity were determined for all 20 leaf extracts. Based on these assays, Glochidion littorale leaf extract (GLE), which showed a high value in all tested parameters, was used in further experiments to evaluate its effects on neurodegeneration in Caenorhabditis elegans. GLE treatment ameliorated H2O2-induced oxidative stress by attenuating the accumulation of reactive oxygen species and protected the worms against 1-methyl-4-phenylpyridinium-induced neurodegeneration. The neuroprotective effects observed may be associated with the activation of the transcription factor DAF-16. The characterization of this extract by LC-MS identified several phenolic compounds, including myricetin, coumestrin, chlorogenic acid, and hesperidin, which may play a key role in neuroprotection. This study reports the novel neuroprotective activity of GLE, which may be used to develop treatments for neurodegenerative diseases such as Parkinson’s syndrome.

scholarly journals In-vitro antioxidant and anti-inflammatory potential of Ethanol extracts (root and aerial parts) of Barleria noctiflora

2018 ◽  
Vol 7 (2) ◽  
pp. 1997
Author(s):  
Manjula M.S. ◽  
Saravana Ganthi
Keyword(s):  
Protein Denaturation ◽  
Radical Scavenging Activity ◽  
Folk Medicine ◽  
Radical Scavenging ◽  
Aerial Parts ◽  
Root Sample ◽  
Anti Inflammatory ◽  
Ethanol Extracts ◽  
High Level

Barleria noctiflora L.f. (Acanthaceae) is widely used as folk medicine. In the present study ethanol extracts of root and aerial parts of Barleria noctiflora were prepared using soxhlet extractor. Anti-oxidant potential was evaluated by free radical scavenging activity on DPPH and in vitro anti- inflammation activity of the selected samples stood determined by inhibition of protein denaturation experiment and inhibition of α-amylase studies. Among the root and aerial parts of Barleria noctiflora samples root sample shows significantly high level of antioxidant and anti-inflammatory activity.

scholarly journals Climate change effects on phytochemical compounds and antioxidant activity of Olea europaea

2020 ◽  
Vol 48 (1) ◽  
pp. 436-455 ◽  
Author(s):  
Samia BEN MANSOUR-GUEDDES ◽  
Dhouha SAIDANA-NAIJA ◽  
Ameni BCHIR ◽  
Mohamed BRAHAM
Keyword(s):  
Oxidative Stress ◽  
Antioxidant Activity ◽  
Radical Scavenging Activity ◽  
Radical Scavenging ◽  
Olive Tree ◽  
Water Shortage ◽  
Climatic Conditions ◽  
Spectrophotometric Analysis ◽  
Antioxidant Compounds ◽  
Steroid Synthesis

To tolerate water shortage and high temperature, olive cv. ‘Meski’, the main variety of table olives in Tunisia, developed several biochemical changes. The hereby study focused on the adaptation of the olive tree to the climatic conditions, considering the evaluation of phenolic compounds, chlorophylls, carotenoids, saponin and steroid synthesis, as well as the evaluation of the antioxidant activity. The analyses were made upon fresh leaves collected from three coastal areas of Tunisia (North (sub-humid), center (higher semi-arid) and South (lower arid)) and using different leaves’ extracts. The results emphasized that Southern ‘Meski’ trees had leaves with more polyphenols, flavonoids, o-diphenols and tannins compared with the Northern ones. From the North to the South, ‘Meski’ leaves showed an increase of carotenoids and a decrease of chlorophyll a and b contents. The highest level of antioxidant compounds of Southern leaves could be contributed to reduce the oxidative stress of the olive tree. The spectrophotometric analysis of the antioxidant capacity of leaves collected from central and Southern areas, based on DPPH and ABTS radical-scavenging activity, showed a higher value of antioxidant activity than the Northern ones, at different extract concentrations. Therefore, the increase of the analyzed bioactive compounds can be considered as a response of the tree to surround aggressions and to oppose the oxidative stress that results from the severity of climatic conditions, characteristic of the Southern area.

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