scholarly journals Cannabinoids and Terpenes as an Antibacterial and Antibiofouling Promotor for PES Water Filtration Membranes

Molecules ◽  
2020 ◽  
Vol 25 (3) ◽  
pp. 691 ◽  
Author(s):  
Ismara Nadir ◽  
Nosheen Fatima Rana ◽  
Nasir Mahmood Ahmad ◽  
Tahreem Tanweer ◽  
Amna Batool ◽  
...  
Keyword(s):  
Contact Angle ◽  
Water Retention ◽  
Hybrid Membrane ◽  
Water Filtration ◽  
Gram Negative Bacteria ◽  
Hybrid Membranes ◽  
Gram Positive ◽  
Gram Negative ◽  
X Ray ◽  
Filtration Membranes

Plant phytochemicals have potential decontaminating properties, however, their role in the amelioration of hydrophobic water filtration membranes have not been elucidated yet. In this work, phytochemicals (i.e., cannabinoids (C) and terpenes (T) from C. sativa) were revealed for their antibacterial activity against different Gram-positive and Gram-negative bacteria. As such, a synergistic relationship was observed between the two against all strains. These phytochemicals individually and in combination were used to prepare polyethersulfone (PES) hybrid membranes. Membrane characterizations were carried out using scanning electron microscopy, Fourier transform infrared spectroscopy, energy-dispersive X-ray spectroscopy. Moreover, contact angle, water retention, surface roughness, mechanical testing, and X-ray florescence analysis were also carried out. According to results, the CT-PES hybrid membrane exhibited the lowest contact angle (40°), the highest water retention (70%), and smallest average pore size (0.04 µm). The hybrid membrane also exhibited improved water flux with no surface leaching. Quantitative bacterial decline analysis of the CT-PES hybrid membranes confirmed an effective antibacterial performance against Gram-positive and Gram-negative bacteria. The results of this study established cannabinoids and terpenes as an inexpensive solution for PES membrane surface modification. These hybrid membranes can be easily deployed at an industrial scale for water filtration purposes.

scholarly journals Rational Design of Ag/ZnO Hybrid Nanoparticles on Sericin/Agarose Composite Film for Enhanced Antimicrobial Applications

2020 ◽  
Vol 22 (1) ◽  
pp. 105
Author(s):  
Wanting Li ◽  
Zixuan Huang ◽  
Rui Cai ◽  
Wan Yang ◽  
Huawei He ◽  
...  
Keyword(s):  
Mechanical Properties ◽  
Antimicrobial Activity ◽  
Composite Film ◽  
Water Absorption ◽  
Rational Design ◽  
Hybrid Nanoparticles ◽  
Gram Negative Bacteria ◽  
Gram Positive ◽  
Gram Negative ◽  
X Ray

Silver-based hybrid nanomaterials are receiving increasing attention as potential alternatives for traditional antimicrobial agents. Here, we proposed a simple and eco-friendly strategy to efficiently assemble zinc oxide nanoparticles (ZnO) and silver nanoparticles (AgNPs) on sericin-agarose composite film to impart superior antimicrobial activity. Based on a layer-by-layer self-assembly strategy, AgNPs and ZnO were immobilized on sericin-agarose films using the adhesion property of polydopamine. Scanning electron microscopy, energy-dispersive X-ray spectroscopy, and X-ray powder diffraction spectroscopy were used to show the morphology of AgNPs and ZnO on the surface of the composite film and analyze the composition and structure of AgNPs and ZnO, respectively. Water contact angle, swelling ratio, and mechanical property were determined to characterize the hydrophilicity, water absorption ability, and mechanical properties of the composite films. In addition, the antibacterial activity of the composite film was evaluated against Gram-positive and Gram-negative bacteria. The results showed that the composite film not only has desirable hydrophilicity, high water absorption ability, and favorable mechanical properties but also exhibits excellent antimicrobial activity against both Gram-positive and Gram-negative bacteria. It has shown great potential as a novel antimicrobial biomaterial for wound dressing, artificial skin, and tissue engineering.

ANTIMICROBIAL ACTIVITY OF COPPER KAOLINITE AND SURFACTANT MODIFIED COPPER KAOLINITE AGAINST GRAM POSITIVE AND GRAM NEGATIVE BACTERIA

2016 ◽  
Vol 78 (3-2) ◽  
Author(s):  
Nor Syafawani Sarah Md Saad ◽  
Nik Ahmad Nizam Nik Malek ◽  
Chun Shiong Chong
Keyword(s):  
Antimicrobial Activity ◽  
Synergistic Effects ◽  
Wide Spectrum ◽  
Copper Nitrate ◽  
Gram Negative Bacteria ◽  
Gram Positive ◽  
Gram Negative ◽  
X Ray ◽  
Gram Positive Bacteria ◽  
Diffusion Technique

The aim of this research was to determine the antimicrobial activity of kaolinite modified with antimicrobial compounds against Gram positive and Gram negative bacteria. Copper kaolinite (Cu-kaolinite) was prepared by loading raw kaolinite with copper nitrate trihydrate (CuNO3) while surfactant modified Cu-kaolinite (SM-Cu-kaolinite) was prepared by adding cationic surfactants hexadecyltrimethyl ammonium (HDTMA) on Cu-Kaolinite. Samples was characterized by Fourier transform infrared spectroscopy (FTIR), X-ray diffraction (XRD), scanning electron microscopy (SEM) and energy dispersive X-ray (EDX) analyzer. The antimicrobial activity of the samples was tested against Gram negative bacteria (Escherichia coli ATCC 11229 and Pseudomonas aeruginosa ATCC 15442), and Gram positive bacteria (Staphylococcus aureus ATCC 6538 and Enterococcus faecalis ATCC 29212) through disc diffusion technique (DDT) and minimum inhibition concentration (MIC). The results showed that the antimicrobial activity of Cu-kaolinite increased after modified with HDTMA due to the synergistic effects of Cu ions and HDTMA molecules on the kaolinite. The antimicrobial activity for surfactant modified Cu-kaolinite was greater for Gram positive bacteria compared to Gram negative bacteria. In conclusion, the attachment of HDTMA on Cu-kaolinite contributed to the enhanced antimicrobial activity against wide spectrum of bacteria (Gram positive and Gram negative bacteria).

Mannich Reaction Product of Quinolin-8-ol (Oxine) and its Antibacterial Activity

1997 ◽  
Vol 50 (8) ◽  
pp. 861 ◽  
Author(s):  
Shaheen Faizi ◽  
Bina Shaheen Siddiqui ◽  
Rubeena Saleem ◽  
Farzana Akhtar ◽  
Khursheed Ali Khan ◽  
...  
Keyword(s):  
Antibacterial Activity ◽  
Single Crystal ◽  
Mannich Reaction ◽  
Gram Negative Bacteria ◽  
X Ray Diffraction ◽  
Gram Positive ◽  
Gram Negative ◽  
Antimicrobial Screening ◽  
X Ray

Quinolin-8-ol (1) on reaction with 2,6-dichloroaniline and m-nitrobenzaldehyde afforded 7-(α-hydroxy- m-nitrobenzyl)quinolin-8-ol (2), which is an unusual Mannich reaction product. The structure of (2) was determined by spectroscopic and single-crystal X-ray diffraction studies. Preliminary antimicrobial screening showed that (1) and (2) have activity against a large number of Gram-positive and Gram-negative bacteria.

Structural comparison of the histidine-containing phosphocarrier protein HPr

1994 ◽  
Vol 72 (5-6) ◽  
pp. 202-217 ◽  
Author(s):  
Zongchao Jia ◽  
J. Wilson Quail ◽  
Louis T. J. Delbaere ◽  
E. Bruce Waygood
Keyword(s):  
Conformational Change ◽  
Active Center ◽  
Resonance Structure ◽  
Nuclear Magnetic Resonance Structure ◽  
Gram Negative Bacteria ◽  
Structural Comparison ◽  
Phosphotransferase System ◽  
Gram Positive ◽  
Gram Negative ◽  
X Ray

The phosphocarrier protein HPr is a central component of the bacterial phosphoenolpyruvate: sugar phosphotransferase system (PTS) that is responsible for carbohydrate uptake in many bacterial species. A number of three-dimensional structures of HPrs from both Gram-positive and Gram-negative bacteria have been determined; the overall folding topology of HPr is an open-faced β-sandwich composed of three α-helices and a β-sheet. A detailed structural comparison of these HPrs has been carried out. Besides the overall main chain folding, many detailed structural features are well conserved in all HPr structures. The three x-ray structures of HPrs from Escherichia coli, Streptococcus faecalis, and Bacillus subtilis show considerable overall similarity with respect to the positions of the Cα atoms. A significant structural difference between HPrs from Gram-positive and Gram-negative bacteria is found in the region of Gly54, owing to the steric effects of Tyr37 in HPrs from the Gram-positive species. The region around Gly54 is involved in the binding of HPr to other PTS proteins and the differences in this region may be responsible for some of the poor functional complementation between HPrs from Gram-positive and Gram-negative species. The active center region, residues 12–18, appears to have significant differences in the comparisons between the overall structures. These differences support the proposal that phosphorylation and dephosphorylation of the active site His 15 is accompanied by conformational changes. However, a local structural comparison of residues 12–18 from the x-ray structures of HPrs from E. coli and B. subtilis, and the two-dimensional nuclear magnetic resonance structure of B. subtilis HPr suggests that there is a conserved active center involving residues His15, Arg 17, and Pro18, which shows little conformational change during the phosphorylation cycle. The results of other experimental approaches, including site-directed mutagenesis and NMR spectroscopy, are in some cases difficult to rationalize with some of the details of the structures, but do appear to favour the conclusion that little conformational change occurs.Key words: phosphoenolpyruvate: sugar phosphotransferase system, histidine-containing protein, active center, phosphorylation, protein structure, structural homology.

Al2O3 Nanopowders, a Suitable Compound for Active Control of Biofouling

2015 ◽  
Vol 32 ◽  
pp. 71-80
Author(s):  
Mohsen Mehregan ◽  
Hossein Soltaninejad ◽  
Behnaz Toluei Nia ◽  
Hadi Zare-Zardini ◽  
Masoud Zare-Shehneh ◽  
...  
Keyword(s):  
Electron Microscopy ◽  
Antibacterial Activity ◽  
Blood Cells ◽  
Gram Negative Bacteria ◽  
Bacterial Strains ◽  
X Ray Diffraction ◽  
Gram Positive ◽  
Gram Negative ◽  
X Ray

The formation of biofilm (Biofouling) in different surface is the great concern in types of fields, especially in medical and health system as well as in membrane technology. The present study deals with the synthesis and characterization of Al2O3 nanopowders with antibacterial activity which can be a potentially utilized material for biocompatible implants. Nanostructure was synthesized based on sol-gel method and then, crystallite size, and microstructural and morphological characterization of nanostructure were determined by X-ray diffraction, electron-microscopic techniques - scanning electron microscopy (SEM) and transmission electron microscopy (TEM). According to X-ray diffraction, the value of particle size for Al2O3 nanopowders is 20.85 nm. In the following, the antibacterial activity of Al2O3 nanoparticles was assessed on three gram positive and three gram negative bacteria by radial diffusion assay and measurement of minimum inhibitory concentration (MIC). The toxicity of Al2O3 nanopowders on blood cells was also assessed. The results showed that this nanostructure has potent antibacterial activity against gram positive and gram negative bacteria. The synthesized Al2O3 nanopowders showed the antimicrobial activity against antibiotic resistant bacterium, Staphylococcus aureus. Significant antibacterial activity of this nanostructure was seen to have a greatest effect on Bacillus cereus with the MIC value of 9.2 μg/ml; while, among bacterial strains, Salmonellatyphimurium was investigated to be the most resistant one with the MIC of 35.6 μg/ml. Al2O3 nanopowders showed no toxicity on blood cells. according to acquired data in this study, Al2O3 nanopowders may be a good material for inhibition of biofilm formation.

Facile Synthesis of SiO2/CMC/Ag Hybrids Derived from Waste Biomass (Sugarcane Bagasse) Having Special Medical Application

2020 ◽  
Vol 20 (10) ◽  
pp. 6413-6421
Author(s):  
Harish Kumar ◽  
Avneesh Kumar Gehlaut ◽  
Ankur Gaur ◽  
Jin-Won Park ◽  
Sanjeev Maken
Keyword(s):  
Sugarcane Bagasse ◽  
Sol Gel ◽  
Ultra Violet ◽  
Gram Negative Bacteria ◽  
Waste Biomass ◽  
Ag Nps ◽  
Gram Positive ◽  
Gram Negative ◽  
X Ray ◽  
Gram Positive Bacteria

This research is focused on the use of sol gel technique to synthesize amorphous SiO2 hybrids derived from lab made CMC (made from sugarcane bagasse) and tetraethoxysilane (TEOS) comprising silver nanoparticles (Ag-NPs). The dominant absorption peak in the order of 425 nm confirms the presence of Ag-NPs hybrid group owing to the surface Plasmon resonance (SPR). Ag-NPs hybrid characterization of was performed by Ultra violet-visible spectra (UV-Vis), Scanning electronic microscopy (SEM), Transmission electron microscopy (TEM), Particle size analyser (PSA), Energy Dispersive X-ray spectroscopy (EDX), X-ray diffractometer (XRD) and Fourier transform infrared spectroscopy (FTIR). The antibacterial action of Ag-NPs in contrast to Gram-negative bacteria (Escherichia coli) (ATCC 433) and Gram-positive bacteria (Bacillus subtilis) (ATCC 1688) was analyzed by using the method of agar disk diffusion technique. Ag-NPs hybrids extracted from lab-made CMC confirm higher adverse bacterial action through Gram-positive bacteria as well as Gram-negative bacteria related to synthetic CMC acquired from the market.

Nano-zirconium oxide and nano-silver oxide/cotton gauze fabrics for antimicrobial and wound healing acceleration

2011 ◽  
Vol 41 (3) ◽  
pp. 222-240 ◽  
Author(s):  
Mohamed Gouda
Keyword(s):  
Zirconium Oxide ◽  
Silver Oxide ◽  
Skin Irritation ◽  
Reduction Rate ◽  
Colony Count ◽  
Gram Negative Bacteria ◽  
Gram Positive ◽  
Gram Negative ◽  
X Ray ◽  
Nano Silver

Nano-zirconium-oxide (nano-Zr-oxide) and nano-silver-oxide (nano-Ag-oxide) were in situ synthesized and deposited into cotton gauze fabrics by reduction of zirconium oxychloride or silver nitrate solutions, in the presence of fabric samples using sodium hydroxide-hydrogen peroxide mixture solution at pH 9.5. The resulted homogenous distribution of nano-Zr-oxide and nano-Ag-oxide inside the fabric were characterized by scanning electron microscopy coupled with energy dispersive X-ray (SEM-EDX), transmission electron microscopy (TEM) for fabric cross-section. The formed nano-Zr-oxide and nano-Ag-oxide in the prepared solution was characterized by TM, X-ray diffraction (XRD), and UV visible spectroscopy. Antibacterial activity of prepared samples was evaluated using reduction rate % in bacterial count (RBC %) against gram-negative bacteria ( Salmonella typhimurium) and gram-positive bacteria ( Staphylococcus aureous), while antifungal activity of prepared samples was evaluated according to clear inhibition zone diameter against filamentous fungus ( Candida albicans), as well as nonfilamentous fungus ( Aspergillus flavus) the data show that, the reduction rate of colony count of treated fabric samples with nano-silver oxide against gram positive and gram negative bacteria were 99.9% and 97% respectively. Gave 99.9% and 97% reduction in colony count against gram-positive and gram-negative, espectively, while sample fabrics treated with nano-Zr-oxide gave 98% and 95% reduction rate in colony count against gram-positive and gram-negative, respectively. Antifungal activity of fabric treated with nano-silver oxide was greater than that of fabrics treated with nano-Zr-oxide. Skin irritation test of treated fabric samples was evaluated and the data show that, there is no skin irritation was observed on the skin of tested rabbit. Skin wound of tested rabbit was healed after 4 days sample application. All prepared samples were durable to wash and killed the bacteria even after 30 laundering wash cycles.

scholarly journals PHOTODYNAMIC RESPONSE IN BACTERIA

1959 ◽  
Vol 42 (5) ◽  
pp. 917-922 ◽  
Author(s):  
C. L. Baugh ◽  
J. B. Clark
Keyword(s):  
Genetic Factors ◽  
Gram Negative Bacteria ◽  
Gram Positive ◽  
Gram Negative ◽  
Photodynamic Action ◽  
X Ray ◽  
Kinetics Of

The photodynamic lethal response of Gram negative bacteria was found to be a one event phenomenon as previously reported. However, the kinetics of the lethal response in Gram-positive microorganisms was found to be more complex. The survivor response of the latter was dividable into at least two distinct parts, each of which could be influenced by various environmental and genetic factors. Some correlation was found between sensitivity or resistance to x-ray and ultraviolet radiations and sensitivity or resistance to photodynamic action.

Rapid demonstration of septic or infectious arthritis due to Gram-negative bacteria

1992 ◽  
Vol 50 (1) ◽  
pp. 686-687
Author(s):  
Jacob S. Hanker ◽  
Paul R. Gross ◽  
Beverly L. Giammara
Keyword(s):  
Chronic Arthritis ◽  
Blood Cultures ◽  
Gram Negative Bacteria ◽  
Infectious Arthritis ◽  
Bacterial Arthritis ◽  
Gram Positive ◽  
Gram Negative ◽  
Gram Positive Bacteria

Blood cultures are positive in approximately only 50 per cent of the patients with nongonococcal bacterial infectious arthritis and about 20 per cent of those with gonococcal arthritis. But the concept that gram-negative bacteria could be involved even in chronic arthritis is well-supported. Gram stains are more definitive in staphylococcal arthritis caused by gram-positive bacteria than in bacterial arthritis due to gram-negative bacteria. In the latter situation where gram-negative bacilli are the problem, Gram stains are helpful for 50% of the patients; they are only helpful for 25% of the patients, however, where gram-negative gonococci are the problem. In arthritis due to gram-positive Staphylococci. Gramstained smears are positive for 75% of the patients.

In-vitro Antioxidant and Antibacterial Activity of Methanolic Extract of Shorea robusta Gaertn. F. Resin

2016 ◽  
Vol 6 (4) ◽  
pp. 68
Author(s):  
Sushma Vashisht ◽  
Manish Pal Singh ◽  
Viney Chawla
Keyword(s):  
Antibacterial Activity ◽  
Methanolic Extract ◽  
Diffusion Method ◽  
Gram Negative Bacteria ◽  
Disc Diffusion ◽  
Gram Positive ◽  
Gram Negative ◽  
Shorea Robusta ◽  
Dose Dependent ◽  
Resin Extract

The methanolic extract of the resin of Shorea robusta was subjected to investigate its antioxidant and antibacterial properties its utility in free radical mediated diseases including diabetic, cardiovascular, cancer etc. The methanol extract of the resin was tested for antioxidant activity using scavenging activity of DPPH (1,1-diphenyl-2-picrylhydrazil) radical method, reducing power by FeCl3 and antibacterial activity against gram positive and gram negative bacteria using disc diffusion method. The phytochemical screening considered the presence of triterpenoids, tannins and flavoniods. Overall, the plant extract is a source of natural antioxidants which might be helpful in preventing the progress of various oxidative stress mediated diseases including aging. The half inhibition concentration (IC50) of resin extract of Shorea robusta and ascorbic acid were 35.60 µg/ml and 31.91 µg/ml respectively. The resin extract exhibit a significant dose dependent inhibition of DPPH activity. Antibacterial activity was observed against gram positive and gram negative bacteria in dose dependent manner.Key Words: Shorea robusta, antioxidant, antibacterial, Disc-diffusion, DPPH.

Sign in / Sign up

Export Citation Format

Share Document