scholarly journals Ceiba speciosa (A. St.-Hil.) Seeds Oil: Fatty Acids Profiling by GC-MS and NMR and Bioactivity

Molecules ◽  
2020 ◽  
Vol 25 (5) ◽  
pp. 1037
Author(s):  
Sergio Rosselli ◽  
Rosa Tundis ◽  
Maurizio Bruno ◽  
Mariarosaria Leporini ◽  
Tiziana Falco ◽  
...  
Keyword(s):  
Antioxidant Activities ◽  
Sterculic Acid ◽  
In Vitro Assays ◽  
Gas Chromatography Mass Spectrometry ◽  
Inhibitory Effects ◽  
Bioactive Constituents ◽  
Reducing Ability ◽  
Ic50 Value ◽  
Β Carotene

This study aimed to evaluate the chemical composition by gas chromatography-mass spectrometry (GC-MS) and Nuclear Magnetic Resonance (NMR) analyses, the antioxidant activities evaluated by different in vitro assays namely 2,2-diphenyl-1-picrylhydrazyl (DPPH), 2,2′-azino-bis(3-ethylbenzothiazoline-6-sulphonic acid) (ABTS), Ferric Reducing Ability Power (FRAP), and β-carotene bleaching tests, and the inhibitory effects of enzymes linked to obesity (lipase, α-amylase, and α-glucosidase) of fixed seed oil of Ceiba speciosa (A. St.-Hil.). Fourteen compounds were identified. Linoleic acid (28.22%) was the most abundant followed by palmitic acid (19.56%). Malvalic acid (16.15%), sterculic acid (11.11%), and dihydrosterculic acid (2.74%) were also detected. C. speciosa fixed oil exerted a promising ABTS radicals scavenging activity with an IC50 value of 10.21 µg/mL, whereas an IC50 of 77.44 µg/mL against DPPH+ radicals was found. C. speciosa fixed oil inhibited lipase with an IC50 value of 127.57 µg/mL. The present investigation confirmed the functional properties of C. speciosa fixed oil, and proposes its use as valuable source of bioactive constituents.

scholarly journals Evaluation of antioxidant activities of saffron stigma and spath as by-product of Crocus sativus L

2018 ◽  
Vol 3 (4) ◽  
pp. 154-158
Author(s):  
Lahmass I ◽  
Ouahhoud S ◽  
Elyoubi M ◽  
Benabbas R ◽  
Sabouni A ◽  
...  
Keyword(s):  
Antioxidant Activities ◽  
Radical Scavenging ◽  
Reducing Power ◽  
Crocus Sativus ◽  
In Vitro Assays ◽  
Antioxidant Compounds ◽  
Total Antioxidant ◽  
Β Carotene ◽  
Crocus Sativus L

The present study aimed to estimate and compare, using in vitro assays, the antioxidant activities of alcoholic extracts of saffron stigma and spath as by-products of the saffron crop. The antioxidant activity was evaluated using in vitro assays such as total antioxidant capacity, DPPH free radical, Scavenging of H2O2, FRAP and β-carotene bleaching assay. The results allowed us to evidence the presence of products with antioxidant and bioactive capacities in spaths and stigmas of Crocus sativus. The response of each extract depends on the type of the test. Extract from spaths protect β-carotene from bleaching more than the extract of stigmas and extract from stigmas revealed a significant reducing power activity than that of spaths. As a result, we can consider these extracts especially that of spaths like a new source of antioxidant compounds.

scholarly journals Antioxidant activities and antidiabetic potential of extract of fruits from the Myrtaceae family: inhibitory effects on α-amylase and α-glucosidase activities

2021 ◽  
Vol 18 (1) ◽  
pp. 25
Author(s):  
Simone Muniz Pacheco ◽  
Rejane Giacomelli Tavares ◽  
Mauricio Seifert ◽  
Leonardo Nora ◽  
Rafael de Almeida Schiavon
Keyword(s):  
Phenolic Compounds ◽  
Antioxidant Activities ◽  
Amylase Activity ◽  
In Vitro Assays ◽  
Phenolic Antioxidants ◽  
Syzygium Cumini ◽  
Inhibitory Effects ◽  
Edible Fruit ◽  
Forest Region

There is a great diversity of plants which are grown in the Atlantic Forest region of Brazil that produce small, colorful, edible fruit that are used in empiric mode to treat several diseases, such as diabetes, as fruits are a rich source of dietary phenolic antioxidants. In this study, we investigated the inhibitory activity of methanolic fruit extracts from the Myrtaceae family - Psidium cattleianum (araçá), Syzygium cumini (jambolão), Campomanesia xanthocarpa (guabiroba), Eugenia uniflora (pitanga) and Eugenia pyriformis (uvaia) - against α-amylase and intestinal α-glucosidase (maltose and sucrose). The antioxidant activities were evaluated using two different in vitro assays: the 2,2’-azinobis(3-ethylbenzthiazoline-6-sulphonate) (ABTS) test and the 2,2’-diphenyl-1-picrylhydrazyl (DPPH) test. The extracts of P. cattleianum, S. cumini, E. pyriformis inhibited α-amylase activity between 13% and 60% (p<0.05). The extracts of P. cattleianum also inhibited α-glucosidase activity with either maltose or sucrose as substrate between 15% and 61% (p<0.05). Additionally, these fruits are rich in phenolic compounds with antioxidant activities.

scholarly journals Retinal Biosynthesis in Fungi: Characterization of the Carotenoid Oxygenase CarX from Fusarium fujikuroi

2007 ◽  
Vol 6 (4) ◽  
pp. 650-657 ◽  
Author(s):  
Alfonso Prado-Cabrero ◽  
Daniel Scherzinger ◽  
Javier Avalos ◽  
Salim Al-Babili
Keyword(s):  
High Performance ◽  
Feedback Mechanism ◽  
In Vitro Assays ◽  
Gas Chromatography Mass Spectrometry ◽  
Carotenoid Content ◽  
Fusarium Fujikuroi ◽  
Negative Feedback Mechanism ◽  
Total Carotenoid Content ◽  
Β Carotene

ABSTRACT The car gene cluster of the ascomycete Fusarium fujikuroi encodes two enzymes responsible for torulene biosynthesis (CarRA and CarB), an opsin-like protein (CarO), and a putative carotenoid cleaving enzyme (CarX). It was presumed that CarX catalyzes the formation of the major carotenoid in F. fujikuroi, neurosporaxanthin, a cleavage product of torulene. However, targeted deletion of carX did not impede neurosporaxanthin biosynthesis. On the contrary, ΔcarX mutants showed a significant increase in the total carotenoid content, indicating an involvement of CarX in the regulation of the pathway. In this work, we investigated the enzymatic activity of CarX. The expression of the enzyme in β-carotene-accumulating Escherichia coli cells led to the formation of the opsin chromophore retinal. The identity of the product was proven by high-performance liquid chromatography and gas chromatography-mass spectrometry. Subsequent in vitro assays with heterologously expressed and purified CarX confirmed its β-carotene-cleaving activity and revealed its capability to produce retinal also from other substrates, such as γ-carotene, torulene, and β-apo-8′-carotenal. Our data indicate that the occurrence of at least one β-ionone ring in the substrate is required for the cleavage reaction and that the cleavage site is determined by the distance to the β-ionone ring. CarX represents the first retinal-synthesizing enzyme reported in the fungal kingdom so far. It seems likely that the formed retinal is involved in the regulation of the carotenoid biosynthetic pathway via a negative feedback mechanism.

scholarly journals Ducrosia ismaelis Asch. essential oil: chemical composition profile and anticancer, antimicrobial and antioxidant potential assessment

2020 ◽  
Vol 18 (1) ◽  
pp. 175-184 ◽  
Author(s):  
Ramzi A. Mothana ◽  
Fahd A. Nasr ◽  
Jamal M. Khaled ◽  
Omar M. Noman ◽  
Nael Abutaha ◽  
...  
Keyword(s):  
Gas Chromatography ◽  
Essential Oil ◽  
Antioxidant Activities ◽  
Radical Scavenging ◽  
Gas Chromatography Mass Spectrometry ◽  
Moderate Activity ◽  
Antioxidant Agent ◽  
Total Oil ◽  
Β Carotene ◽  
Mcf 7

AbstractThe essential oil of Ducrosia ismaelis Asch. (Apiaceae) that grows wild in Saudi Arabia was investigated utilizing gas chromatography (GC), and gas chromatography-mass spectrometry. Fifty constituents were characterized, representing 96.1% of the total oil. The D. ismaelis essential oil (DIEO) was distinguished by a high composition of oxygenated monoterpenes (51.6%). Decanal (40.6%), α-pinene (15.1%) and dodecanal (13.7%) were the fundamental components. Additionally, DIEO was evaluated for its cytotoxic, antibacterial, antifungal and antioxidant activities. DIEO revealed a great cytotoxic effectiveness against the tested cancer cell lines with IC50 values between 66.2 and 137.3 μg/mL particularly against MCF-7 cancer cells. Furthermore, the induction of apoptosis against MCF-7 cells has been asserted using staining assay (annexin VFITC and/or propidium iodide (PI) dyes) and flow cytometry technique. The DIEO possessed a strong antimicrobial activity against Gram-positive bacterial and fungal strains with MIC-values between 0.07 and 0.31 mg/ml. The values of MBC or MFC were almost once higher than those of MIC’s. Moreover, the β-carotene-bleaching and DPPH free radical-scavenging tests showed that DIEO had a moderate activity (68%) as an antioxidant agent in decolouring of the β-carotene at 1.0 mg/mL and a moderate radical scavenging for DPPH (66 and 72%) at 0.50 and 1.0 mg/mL.

In Vitro Antioxidant Activity Metaplexis Japonica Makino Extract and Derivatives

2014 ◽  
Vol 955-959 ◽  
pp. 387-389 ◽  
Author(s):  
Bao Qing Wang
Keyword(s):  
Antioxidant Activity ◽  
Linoleic Acid ◽  
Ethyl Acetate ◽  
Antioxidant Activities ◽  
Radical Scavenging ◽  
Acid Test ◽  
Acetone Extracts ◽  
Β Carotene ◽  
Ethyl Acetate Extracts

Antioxidant activities of acetone and ethyl acetate extracts from Metaplexis japonica Makino, one of famous medicine plants in the eastnorth region of China, named luomo in Chinese, were examined by a DPPH (1,1-Diphenyl-2-picrylhydrazyl) radical-scavenging assay and a β-carotene-linoleic acid test. In DPPH, the antioxidant activity of the acetone extracts, ethyl acetate extracts and derivative were IC50 were 313.21, 266.92 and 118.78μg/mL, respectively. In the β-carotene-linoleic acid test, IC50 were 285.09, 351.57 and 123.89μg/mL. It was concluded that Metaplexis japonica Makino and its derivatives might be a potential natural source of antioxidants .

ASSESSMENT OF PHYTOCHEMICALS, TOTAL PHENOL, FLAVONOID CONTENT AND IN VITRO ANTIOXIDANT PROPERTY OF LARGE CARDAMOM EXTRACTS

INDIAN DRUGS ◽  
2021 ◽  
Vol 58 (10) ◽  
pp. 34-41
Author(s):  
Kanthlal S. K. ◽  
Jipnomon Joseph ◽  
Bindhu P. Paul ◽  
Vijayakumar M ◽  
Rema Shree A. B. ◽  
...  
Keyword(s):  
Antioxidant Activity ◽  
Antioxidant Activities ◽  
Radical Scavenging ◽  
Reducing Power ◽  
Antioxidant Property ◽  
Bioactive Constituents ◽  
Preliminary Assessment ◽  
Reducing Power Assay ◽  
Large Cardamom

Amomum subulatum, commonly known as large or black cardamom, is a commonly used spice in Indian kitchens and is traditionally used to treat various ailments. To add more knowledge about the medicinal values of the fruit, this study was conducted to evaluate the in vitro antioxidant activities of aqueous, methanol, ethanol, hydro alcohol, ethyl acetate, acetone and chloroform extracts of the fruit. Preliminary assessment was done to detect the presence of phytoconstituents using identification tests. The antioxidant activity was measured by employing methods such as diphenylpicrylhydrazyl (DPPH) radical scavenging assay, total antioxidant activity equivalent to ascorbic acid, reducing power assay and superoxide anion scavenging assay. The antioxidant activities were compared with their respective phenol and flavonoid contents. Preliminary assessment revealed that large cardamom fruit is a good source of all the bioactive constituents as well as phenol and flavonoid essential for medicinal values. The extract obtained by polar solvents showed the highest antioxidant efficacy in relation to its phenol content. Also, all the solvent-soluble fractions showed a concentration-dependent antioxidant effect. Results from our study prove that large cardamom can alleviate oxidative stress, suggesting the potential of large cardamom as a functional food

Paeonia arietina and Paeonia kesrounansis bioactive constituents: NMR, LC-DAD-MS fingerprinting and in vitro assays

2019 ◽  
Vol 165 ◽  
pp. 1-11 ◽  
Author(s):  
Stefania Sut ◽  
Gokhan Zengin ◽  
Stefano Dall’Acqua ◽  
Markéta Gazdová ◽  
Karel Šmejkal ◽  
...  
Keyword(s):  
In Vitro Assays ◽  
Bioactive Constituents

scholarly journals Porphyra yezoensis Sauces Fermented With Lactic Acid Bacteria: Fermentation Properties, Flavor Profile, and Evaluation of Antioxidant Capacity in vitro

2022 ◽  
Vol 8 ◽  
Author(s):  
Jie Yang ◽  
Tengqi Gao ◽  
Feng Ge ◽  
Hao Sun ◽  
Zihang Cui ◽  
...  
Keyword(s):  
Lactic Acid ◽  
Lactic Acid Bacteria ◽  
Antioxidant Capacity ◽  
Porphyra Yezoensis ◽  
Streptococcus Thermophilus ◽  
Gas Chromatography Mass Spectrometry ◽  
Reducing Ability ◽  
Volatile Flavor ◽  
Dpph Scavenging

The demand for roasted seaweed sandwich (Porphyra yezoensis) product has risen in recent years. The product slicing process has created a huge number of scraps that are not utilized effectively. Three lactic acid bacteria (LAB) strains were used to ferment P. yezoensis sauces in this study, including Lactobacillus fermentum, Lactobacillus casei, Streptococcus thermophilus, and the mixed strains (1:1:1, v/v). The fermentation characteristics, antioxidant capacity in vitro, sensory properties, and flavoring substances of fermented P. yezoensis sauces were analyzed. After 21 days of fermentation, all LAB strains grew well in the P. yezoensis sauces, with protease activity increased to 6.6, 9.24, 5.06, and 5.5 U/mL, respectively. Also, the flavors of P. yezoensis sauces fermented with L. casei and L. fermentum were satisfactory. On this premise, gas chromatography-mass spectrometry (GC-MS) was used to investigate the changes in gustatory compounds in P. yezoensis sauces fermented with L. casei and L. fermentum. In general, 42 and 41 volatile flavor chemicals were identified after the fermentation of L. casei and L. fermentum. Furthermore, the fermented P. yezoensis sauce possessed greater DPPH scavenging activity and ferric-reducing ability power than the unfermented P. yezoensis. Overall, the flavor and taste of P. yezoensis sauce fermented by L. casei was superior.

scholarly journals THE ANTIOXIDANT STUDIES OF TWO MEDICINAL PLANTS, SPHAERANTHUS INDICUS AND PSOPHOCARPUS TETRAGONOLOBUS

2019 ◽  
Vol 12 (1) ◽  
pp. 321
Author(s):  
Vijayalakshmi N ◽  
Mudiganti Ram Krishna Rao
Keyword(s):  
Ascorbic Acid ◽  
Medicinal Plants ◽  
Antioxidant Activities ◽  
Psophocarpus Tetragonolobus ◽  
Leaf Extracts ◽  
Frap Assay ◽  
Methanol Fraction ◽  
Reducing Ability ◽  
Ic50 Value ◽  
Sphaeranthus Indicus

Objective: The present study deals with the antioxidant assays of the different leaf extracts of two medicinal plants, Sphaeranthus indicus and Psophocarpus tetragonolobus. Methods: Dried leaves of S. indicus and P. tetragonolobus were packed in separate round bottom flasks for sample extraction using ethanol, methanol, hexane, and distilled water as solvents for 72 h, and the extracts were collected after evaporating the solvents. Antioxidant studies of the various extracts were performed by 1-diphenyl-2-picrylhydrazyl and Ferric Reducing Ability of Plasma assays.Results: Among the two plants studied, S. indicus showed better 2-diphenyl-1-picryl-hydrazyl (DPPH), scavenging activity than P. tetragonolobus with IC50 values of 174.380 and 262.313, respectively, as compared to that of the standard, ascorbic acid, IC50 value of which being 111.16. The FRAP assay results for both the plants indicated that the methanol fractions showed closer results when compared with standards, ascorbic acid and quercetin. The IC50 value of S. indicus, P tetragonolobus, ascorbic acid, and quercetin was 70.065, 151.953, 85.162, and 79.647, respectively. These results clearly indicate that S. indicus methanol fraction had better antioxidant activity when compared to both standards.Conclusion: It is concluded that S. indicus and P. tetragonolobus have excellent antioxidant activities which could be the major contributing factors for their medicinal roles. Further studies in this direction are being carried on.

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