Designing Efficient Processes for Sustainable Bioethanol and Bio-Hydrogen Production from Grass Lawn Waste

The effect of thermal, acid and alkali pretreatment methods on biological hydrogen (BHP) and bioethanol production (BP) from grass lawn (GL) waste was investigated, under different process schemes. BHP from the whole pretreatment slurry of GL was performed through mixed microbial cultures in simultaneous saccharification and fermentation (SSF) mode, while BP was carried out through the C5yeast Pichia stipitis, in SSF mode. From these experiments, the best pretreatment conditions were determined and the efficiencies for each process were assessed and compared, when using either the whole pretreatment slurry or the separated fractions (solid and liquid), the separate hydrolysis and fermentation (SHF) or SSF mode, and especially for BP, the use of other yeasts such as Pachysolen tannophilus or Saccharomyces cerevisiae. The experimental results showed that pretreatment with 10 gH2SO4/100 g total solids (TS) was the optimum for both BHP and BP. Separation of solid and liquid pretreated fractions led to the highest BHP (270.1 mL H2/g TS, corresponding to 3.4 MJ/kg TS) and also BP (108.8 mg ethanol/g TS, corresponding to 2.9 MJ/kg TS) yields. The latter was achieved by using P. stipitis for the fermentation of the hydrolysate and S. serevisiae for the solid fraction fermentation, at SSF.

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A Comparison of the Bioethanol Production from Suweg (Amorphophallus campanulatus) through Separate Hydrolysis and Fermentation as well as Simultaneous Saccharification and Fermentation Using Saccharomyces cerevisiae

IOP Conference Series Materials Science and Engineering ◽

10.1088/1757-899x/1053/1/012036 ◽

2021 ◽

Vol 1053 (1) ◽

pp. 012036

Author(s):

H Hargono ◽

B Jos ◽

P Purwanto ◽

S Sumardiono ◽

MF Zakaria

Keyword(s):

Saccharomyces Cerevisiae ◽

Simultaneous Saccharification And Fermentation ◽

Bioethanol Production ◽

Separate Hydrolysis And Fermentation ◽

Simultaneous Saccharification

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Efficient fractionation of corn stover by bisulfite pretreatment for the production of bioethanol and high value products

BioResources ◽

10.15376/biores.14.3.6501-6515 ◽

2019 ◽

Vol 14 (3) ◽

pp. 6501-6515

Author(s):

Liping Tan ◽

Zhongyang Liu ◽

Tongjun Liu ◽

Fangfang Wang

Keyword(s):

Corn Stover ◽

Simultaneous Saccharification And Fermentation ◽

Bioethanol Production ◽

Different Types ◽

Pretreatment Conditions ◽

C 30 ◽

Hemicellulosic Sugars ◽

Resin Separation ◽

Simultaneous Saccharification

Fractionation of corn stover (CS) was carried out by bisulfite pretreatment in order to improve the production of bioethanol and high-value chemicals. Firstly, the optimum bisulfite pretreatment conditions of CS (170 C, 30 min, 7% NaHSO3, 1% H2SO4) were identified. Next, a biorefinery process of bisulfite pretreatment for CS was proposed. CS was separated into solid and liquor components using such pretreatment. The solid components were employed for bioethanol production by quasi-simultaneous saccharification and fermentation (Q-SSF). The bisulfite liquor was fractionated into hemicellulosic sugars and lignin by different types of resins. It was shown that CS components could be effectively fractionated through bisulfite pretreatment in combination with resin separation to produce bioethanol, hemicellulosic sugars, and lignosulfonate.

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Bioethanol Production from Sugarcane Molasses with Simultaneous Saccharification and Fermentation (SSF) Method using Saccaromyces cerevisiae-Pichia stipitis Consortium

IOP Conference Series Earth and Environmental Science ◽

10.1088/1755-1315/207/1/012061 ◽

2018 ◽

Vol 207 ◽

pp. 012061

Author(s):

Audiananti Meganandi Kartini ◽

Yeny Dhokhikah

Keyword(s):

Simultaneous Saccharification And Fermentation ◽

Pichia Stipitis ◽

Sugarcane Molasses ◽

Bioethanol Production ◽

Saccaromyces Cerevisiae ◽

Simultaneous Saccharification

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Separate hydrolysis and fermentation and simultaneous saccharification and fermentation methods in bioethanol production and formation of volatile by-products from selected corn cultivars

Industrial Crops and Products ◽

10.1016/j.indcrop.2018.03.059 ◽

2018 ◽

Vol 118 ◽

pp. 355-361 ◽

Cited By ~ 13

Author(s):

Katarzyna Szambelan ◽

Jacek Nowak ◽

Artur Szwengiel ◽

Henryk Jeleń ◽

Grzegorz Łukaszewski

Keyword(s):

Simultaneous Saccharification And Fermentation ◽

Bioethanol Production ◽

Separate Hydrolysis And Fermentation ◽

By Products ◽

Simultaneous Saccharification

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Bioethanol production from lignocellulosic feedstock using aqueous ammonia pretreatment and simultaneous saccharification and fermentation (SSF): process development and optimization

10.31274/etd-180810-551 ◽

2010 ◽

Author(s):

Xuan Li

Keyword(s):

Simultaneous Saccharification And Fermentation ◽

Process Development ◽

Aqueous Ammonia ◽

Bioethanol Production ◽

Lignocellulosic Feedstock ◽

Ammonia Pretreatment ◽

Ssf Process ◽

Simultaneous Saccharification

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Production and optimization of cellulase from agricultural waste and its application in bioethanol production by simultaneous saccharification and fermentation

Management of Environmental Quality An International Journal ◽

10.1108/meq-07-2015-0128 ◽

2016 ◽

Vol 27 (1) ◽

pp. 22-35 ◽

Cited By ~ 2

Author(s):

Elsa Cherian ◽

M. Dharmendira Kumar ◽

G. Baskar

Keyword(s):

Design Methodology ◽

Simultaneous Saccharification And Fermentation ◽

Agricultural Waste ◽

Cellulase Production ◽

Reducing Sugar ◽

Bioethanol Production ◽

Corn Cob ◽

Content Type ◽

Optimized Conditions ◽

Simultaneous Saccharification

Purpose – The purpose of this paper is to optimize production of cellulase enzyme from agricultural waste by using Aspergillus fumigatus JCF. The study also aims at the production of bioethanol using cellulase and yeast. Design/methodology/approach – Cellulase production was carried out using modified Mandel’s medium. The optimization of the cellulase production was carried out using Plackett-Burman and Response surface methodology. Bioethanol production was carried out using simultaneous saccharification and fermentation. Findings – Maximum cellulase production at optimized conditions was found to be 2.08 IU/ml. Cellulase was used for the saccharification of three different feed stocks, i.e. sugar cane leaves, corn cob and water hyacinth. Highest amount of reducing sugar was released was 29.1 gm/l from sugarcane leaves. Sugarcane leaves produced maximum bioethanol concentration of 9.43 g/l out of the three substrates studied for bioethanol production. Originality/value – The present study reveals that by using the agricultural wastes, cellulase production can be economically increased thereby bioethanol production.

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Ethanol Production from Corn Stover Using Soaking Pretreatment

Advanced Materials Research ◽

10.4028/www.scientific.net/amr.171-172.261 ◽

2010 ◽

Vol 171-172 ◽

pp. 261-265

Author(s):

Zhuang Zuo ◽

Xiu Shan Yang

Keyword(s):

Ethanol Production ◽

Corn Stover ◽

Simultaneous Saccharification And Fermentation ◽

Pichia Stipitis ◽

Liquid Ratio ◽

Mild Conditions ◽

Whole Process ◽

Conversion Rates ◽

Mild Temperature ◽

Simultaneous Saccharification

Corn stover was pretreated using different soaking conditions at mild temperature. Among the tested conditions, the best was 1% NaOH+8% NH4OH，50°C，48 h, Solid-to-liquid ratio 1:10. The results showed that soaking pretreatment achieved 63.6% deligniﬁcation, retained the xylan and glucan. After enzymatic hydrolysis, conversion rates of xylan and glucan were 70.9% and 78.5%, respectively. The pretreated filtration re-soaking cause 52.7% xylan and 65.0% glucan conversion. NaOH+NH4OH treatment can be performed under mild conditions, gives a good buffering effect, low carbohydates degradation and extensive removal of lignin. Additionally, simultaneous sacchariﬁcation and fermentation was conducted with pretreated corn stover to assess the ethanol production. For the whole process, 0.15g ethanol /g corn stover was achieved using Saccharomyces cerevisiae Y5, and 0.19g ethanol /g corn stover when using Pichia stipitis.

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A study on alkali pretreatment conditions of sorghum stem for maximum sugar recovery using statistical approach

Chemical Industry and Chemical Engineering Quarterly ◽

10.2298/ciceq120826008n ◽

2014 ◽

Vol 20 (2) ◽

pp. 261-271 ◽

Cited By ~ 6

Author(s):

Maryam Nikzad ◽

Kamyar Movagharnejad ◽

Farid Talebnia ◽

Ghasem Najafpour ◽

Farahi Hosein

Keyword(s):

Sorghum Bicolor ◽

Alternative Energy ◽

Solid Loading ◽

Bioethanol Production ◽

Alkali Pretreatment ◽

Model Predictions ◽

Pretreatment Time ◽

Pretreatment Conditions ◽

Energy Production System ◽

Central Composite

Bioethanol production from lignocellulosic biomass provides an alternative energy-production system. Sorghum bicolor stem is a cheap agro-waste for bioethanol production. In this study, response surface methodology (RSM) was used to optimize alkali pretreatment conditions for sorghum bicolor stem with respect to substrate concentration, NaOH concentration and pretreatment time based on a central composite rotary design. The main goal was to achieve the highest glucose and xylose yields after enzymatic hydrolysis. Under optimum conditions of pretreatment i.e. time 60.4 min, solid loading 4.2%, and NaOH concentration 1.7%, yields of 98.94% g glucose/g cellulose and 65.14% g xylose/g hemicelluloses were obtained. The results of a confirmation experiment under the optimal conditions agreed well with model predictions. Pretreatment of sorghum bicolor stem at the optimum condition increased the glucose and xylose yields by 7.14 and 3.02 fold, respectively. Alkali pretreatment showed to be a great choice for the pretreatment of sorghum bicolor stem.

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