scholarly journals A study on alkali pretreatment conditions of sorghum stem for maximum sugar recovery using statistical approach

2014 ◽  
Vol 20 (2) ◽  
pp. 261-271 ◽  
Author(s):  
Maryam Nikzad ◽  
Kamyar Movagharnejad ◽  
Farid Talebnia ◽  
Ghasem Najafpour ◽  
Farahi Hosein
Keyword(s):  
Sorghum Bicolor ◽  
Alternative Energy ◽  
Solid Loading ◽  
Bioethanol Production ◽  
Alkali Pretreatment ◽  
Model Predictions ◽  
Pretreatment Time ◽  
Pretreatment Conditions ◽  
Energy Production System ◽  
Central Composite

Bioethanol production from lignocellulosic biomass provides an alternative energy-production system. Sorghum bicolor stem is a cheap agro-waste for bioethanol production. In this study, response surface methodology (RSM) was used to optimize alkali pretreatment conditions for sorghum bicolor stem with respect to substrate concentration, NaOH concentration and pretreatment time based on a central composite rotary design. The main goal was to achieve the highest glucose and xylose yields after enzymatic hydrolysis. Under optimum conditions of pretreatment i.e. time 60.4 min, solid loading 4.2%, and NaOH concentration 1.7%, yields of 98.94% g glucose/g cellulose and 65.14% g xylose/g hemicelluloses were obtained. The results of a confirmation experiment under the optimal conditions agreed well with model predictions. Pretreatment of sorghum bicolor stem at the optimum condition increased the glucose and xylose yields by 7.14 and 3.02 fold, respectively. Alkali pretreatment showed to be a great choice for the pretreatment of sorghum bicolor stem.

scholarly journals Designing Efficient Processes for Sustainable Bioethanol and Bio-Hydrogen Production from Grass Lawn Waste

Molecules ◽  
2020 ◽  
Vol 25 (12) ◽  
pp. 2889
Author(s):  
Georgia Antonopoulou
Keyword(s):  
Simultaneous Saccharification And Fermentation ◽  
Pichia Stipitis ◽  
Bioethanol Production ◽  
Alkali Pretreatment ◽  
Pachysolen Tannophilus ◽  
Microbial Cultures ◽  
Separate Hydrolysis And Fermentation ◽  
Mixed Microbial Cultures ◽  
Pretreatment Conditions ◽  
Simultaneous Saccharification

The effect of thermal, acid and alkali pretreatment methods on biological hydrogen (BHP) and bioethanol production (BP) from grass lawn (GL) waste was investigated, under different process schemes. BHP from the whole pretreatment slurry of GL was performed through mixed microbial cultures in simultaneous saccharification and fermentation (SSF) mode, while BP was carried out through the C5yeast Pichia stipitis, in SSF mode. From these experiments, the best pretreatment conditions were determined and the efficiencies for each process were assessed and compared, when using either the whole pretreatment slurry or the separated fractions (solid and liquid), the separate hydrolysis and fermentation (SHF) or SSF mode, and especially for BP, the use of other yeasts such as Pachysolen tannophilus or Saccharomyces cerevisiae. The experimental results showed that pretreatment with 10 gH2SO4/100 g total solids (TS) was the optimum for both BHP and BP. Separation of solid and liquid pretreated fractions led to the highest BHP (270.1 mL H2/g TS, corresponding to 3.4 MJ/kg TS) and also BP (108.8 mg ethanol/g TS, corresponding to 2.9 MJ/kg TS) yields. The latter was achieved by using P. stipitis for the fermentation of the hydrolysate and S. serevisiae for the solid fraction fermentation, at SSF.

scholarly journals Improved Glucose Recovery from Sicyos angulatus by NaOH Pretreatment and Application to Bioethanol Production

Processes ◽  
2021 ◽  
Vol 9 (2) ◽  
pp. 245
Author(s):  
Hyung-Eun An ◽  
Kang Hyun Lee ◽  
Ye Won Jang ◽  
Chang-Bae Kim ◽  
Hah Young Yoo
Keyword(s):  
Saccharomyces Cerevisiae ◽  
Alternative Energy ◽  
Invasive Plant ◽  
Food Resource ◽  
Raw Material ◽  
Control Group ◽  
Bioethanol Production ◽  
Naoh Pretreatment ◽  
Glucose Recovery ◽  
Harmful Species

As greenhouse gases and environmental pollution become serious, the demand for alternative energy such as bioethanol has rapidly increased, and a large supply of biomass is required for bioenergy production. Lignocellulosic biomass is the most abundant on the planet and a large part of it, the second-generation biomass, has the advantage of not being a food resource. In this study, Sicyos angulatus, known as an invasive plant (harmful) species, was used as a raw material for bioethanol production. In order to improve enzymatic hydrolysis, S. angulatus was pretreated with different NaOH concentration at 121 °C for 10 min. The optimal NaOH concentration for the pretreatment was determined to be 2% (w/w), and the glucan content (GC) and enzymatic digestibility (ED) were 46.7% and 55.3%, respectively. Through NaOH pretreatment, the GC and ED of S. angulatus were improved by 2.4-fold and 2.5-fold, respectively, compared to the control (untreated S. angulatus). The hydrolysates from S. angulatus were applied to a medium for bioethanol fermentation of Saccharomyces cerevisiae K35. Finally, the maximum ethanol production was found to be 41.3 g based on 1000 g S. angulatus, which was 2.4-fold improved than the control group.

Alkali pretreatment of wheat straw followed by microbial hydrolysis for bioethanol production

2017 ◽  
Vol 40 (9) ◽  
pp. 1203-1211 ◽  
Author(s):  
Bahiru Tsegaye ◽  
Chandrajit Balomajumder ◽  
Partha Roy
Keyword(s):  
Wheat Straw ◽  
Bioethanol Production ◽  
Alkali Pretreatment ◽  
Microbial Hydrolysis

scholarly journals BIOETHANOL PRODUCTION FROM GRAIN SORGHUM (Sorghum bicolor L. Moench) AT LABORATORY SCALE

2019 ◽  
Vol 29 (1) ◽  
pp. 11-17
Author(s):  
Picca Aurora ◽  
◽  
Mirta Castaño ◽  
Julián Isasti ◽  
María Pereyra Cardozo ◽  
...  
Keyword(s):  
Sorghum Bicolor ◽  
Grain Sorghum ◽  
Laboratory Scale ◽  
Bioethanol Production ◽  
Sorghum Bicolor L

Changes in poplar (Populus trichocarpa) wood porous structure after liquid hot water (LHW) pretreatment

2020 ◽  
Vol 112 ◽  
pp. 71-78
Author(s):  
Florentyna Akus-Szyblerg ◽  
Jan Szadkowski ◽  
Andrzej Antczak ◽  
Janusz Zawadzki
Keyword(s):  
Enzymatic Hydrolysis ◽  
Porous Structure ◽  
Populus Trichocarpa ◽  
Hot Water ◽  
Size Exclusion ◽  
Bioethanol Production ◽  
Poplar Wood ◽  
Liquid Hot Water ◽  
Exclusion Chromatography ◽  
Pretreatment Conditions

Changes in poplar (Populus trichocarpa) wood porous structure after liquid hot water (LHW) pretreatment. The aim of this research was to investigate the effect of applying different hydrothermal pretreatment conditions on the porous structure of poplar wood. Porosity is recognised as an important factor considering efficiency of an enzymatic hydrolysis as a step of bioethanol production. Native poplar wood as well as solid fractions after pretreatment performed at different temperatures (160 °C, 175 °C and 190 °C) were analysed. Porous structure was examined with an inverse size-exclusion chromatography (ISEC) method. Results indicated a significant development of the porous structure of the biomass with increasing porosity along with the growing temperature of the LHW process. The temperature of 190 °C was chosen as the most promising condition of poplar wood LHW pretreatment in terms of the efficiency of the subsequent steps of bioethanol production. The obtained results were consistent with the previous experimental data procured during analysis of the LHW pretreated poplar wood and its subsequent enzymatic hydrolysis yield.

scholarly journals Optimization of alkali pretreatment of wheat straw to be used as substrate for biofuels production  

2013 ◽  
Vol 59 (No. 12) ◽  
pp. 537-542 ◽  
Author(s):  
K. Jaisamut ◽  
L. Paulová ◽  
P. Patáková ◽  
M. Rychtera ◽  
K. Melzoch
Keyword(s):  
Response Surface Methodology ◽  
Enzymatic Hydrolysis ◽  
Sodium Hydroxide ◽  
Wheat Straw ◽  
Response Surface ◽  
Fermentable Sugars ◽  
Alkali Pretreatment ◽  
The Media ◽  
Pretreatment Conditions ◽  
Expert Designer

Alkali pretreatment of wheat straw was optimized by response surface methodology to maximize yields of fermentable sugars in subsequent enzymatic hydrolysis and to remove maximum lignin in order to improve rheological attributes of the media. The effects of pretreatment conditions on biomass properties were studied using the Expert Designer software. Concentration of sodium hydroxide and temperature were the factors most affecting pretreatment efficiency. At the optimum (80°C, 39 min, 0.18 g NaOH and 0.06 g lime per g of raw biomass), 93.1 ± 1.0% conversion of cellulose to glucose after enzymatic hydrolysis and 80.3 ± 1.2% yield of monosaccharides (glucose plus xylose and arabinose) from cellulose and hemicellulose of wheat straw were achieved.

scholarly journals Origin, Impact and Control of Lignocellulosic Inhibitors in Bioethanol Production—A Review

Energies ◽  
2020 ◽  
Vol 13 (18) ◽  
pp. 4751
Author(s):  
Nikki Sjulander ◽  
Timo Kikas
Keyword(s):  
Negative Influence ◽  
Inhibition Mechanism ◽  
Bioethanol Production ◽  
Negative Effects ◽  
Lignocellulosic Hydrolysates ◽  
Cell Structures ◽  
Vital Cell ◽  
Pretreatment Conditions ◽  
And Control ◽  
Structural Polymers

Bioethanol production from lignocellulosic biomass is still struggling with many obstacles. One of them is lignocellulosic inhibitors. The aim of this review is to discuss the most known inhibitors. Additionally, the review addresses different detoxification methods to degrade or to remove inhibitors from lignocellulosic hydrolysates. Inhibitors are formed during the pretreatment of biomass. They derive from the structural polymers-cellulose, hemicellulose and lignin. The formation of inhibitors depends on the pretreatment conditions. Inhibitors can have a negative influence on both the enzymatic hydrolysis and fermentation of lignocellulosic hydrolysates. The inhibition mechanisms can be, for example, deactivation of enzymes or impairment of vital cell structures. The toxicity of each inhibitor depends on its chemical and physical properties. To decrease the negative effects of inhibitors, different detoxification methods have been researched. Those methods focus on the chemical modification of inhibitors into less toxic forms or on the separation of inhibitors from lignocellulosic hydrolysates. Each detoxification method has its limitations on the removal of certain inhibitors. To choose a suitable detoxification method, a deep molecular understanding of the inhibition mechanism and the inhibitor formation is necessary.

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