scholarly journals Detection of Biomarkers Relating to Quality and Differentiation of Some Commercially Significant Whole Fish Using Spatially Off-Set Raman Spectroscopy

Molecules ◽  
2020 ◽  
Vol 25 (17) ◽  
pp. 3776
Author(s):  
Jeremy D. Landry ◽  
Peter J. Torley ◽  
Ewan W. Blanch
Keyword(s):  
Raman Spectroscopy ◽  
Food Production ◽  
Analytical Techniques ◽  
Label Free ◽  
Analysis Techniques ◽  
Chemical Analysis Techniques ◽  
Aquaculture Industry ◽  
Detection Of Biomarkers ◽  
Non Destructive

Aquaculture represents a major part of the world’s food supply. This area of food production is developing rapidly, and as such the tools and analytical techniques used to monitor and assess the quality of fish need to also develop and improve. The use of spatially off-set Raman spectroscopy (SORS) is particularly well-suited for these applications, given the ability of this technique to take subsurface measurements as well as being rapid, non-destructive and label-free compared to classical chemical analysis techniques. To explore this technique for analysing fish, SORS measurements were taken on commercially significant whole fish through the skin in different locations. The resulting spectra were of high quality with subsurface components such as lipids, carotenoids, proteins and guanine from iridophore cells clearly visible in the spectra. These spectral features were characterised and major bands identified. Chemometric analysis additionally showed that clear differences are present in spectra not only from different sections of a fish but also between different species. These results highlight the potential application for SORS analysis for rapid quality assessment and species identification in the aquaculture industry by taking through-skin measurements.

scholarly journals Nanoscale mapping of newly-synthesised phospholipid molecules in a biological cell using tip-enhanced Raman spectroscopy

2017 ◽  
Vol 53 (16) ◽  
pp. 2451-2454 ◽  
Author(s):  
Naresh Kumar ◽  
Marek M. Drozdz ◽  
Haibo Jiang ◽  
Daniela M. Santos ◽  
David J. Vaux
Keyword(s):  
Raman Spectroscopy ◽  
Biological Cell ◽  
Label Free ◽  
Tip Enhanced Raman Spectroscopy ◽  
First Time ◽  
Non Destructive

Non-destructive & label-free nanoscale mapping of newly-synthesised phospholipid molecules inside a biological cell is demonstrated using tip-enhanced Raman spectroscopy for the first time.

Exploring the structure and formation mechanism of amyloid fibrils by Raman spectroscopy: a review

The Analyst ◽  
2015 ◽  
Vol 140 (15) ◽  
pp. 4967-4980 ◽  
Author(s):  
Dmitry Kurouski ◽  
Richard P. Van Duyne ◽  
Igor K. Lednev
Keyword(s):  
Raman Spectroscopy ◽  
Formation Mechanism ◽  
Structural Characterization ◽  
Amyloid Fibrils ◽  
Label Free ◽  
Amyloidogenic Proteins ◽  
Non Destructive

Applications of Raman spectroscopy, a label-free non-destructive technique, for the structural characterization of amyloidogenic proteins, prefibrilar oligomers, and mature fibrils.

scholarly journals Raman spectroscopy enables phenotyping and assessment of nutrition values of plants: a review

Plant Methods ◽  
2021 ◽  
Vol 17 (1) ◽  
Author(s):  
William Z. Payne ◽  
Dmitry Kurouski
Keyword(s):  
Raman Spectroscopy ◽  
Abiotic Stresses ◽  
Molecular Techniques ◽  
Plant Diseases ◽  
Label Free ◽  
Breeding Programs ◽  
Non Invasive ◽  
Nutrition Value ◽  
Non Destructive ◽  
Selection Of

AbstractOur civilization has to enhance food production to feed world’s expected population of 9.7 billion by 2050. These food demands can be met by implementation of innovative technologies in agriculture. This transformative agricultural concept, also known as digital farming, aims to maximize the crop yield without an increase in the field footprint while simultaneously minimizing environmental impact of farming. There is a growing body of evidence that Raman spectroscopy, a non-invasive, non-destructive, and laser-based analytical approach, can be used to: (i) detect plant diseases, (ii) abiotic stresses, and (iii) enable label-free phenotyping and digital selection of plants in breeding programs. In this review, we critically discuss the most recent reports on the use of Raman spectroscopy for confirmatory identification of plant species and their varieties, as well as Raman-based analysis of the nutrition value of seeds. We show that high selectivity and specificity of Raman makes this technique ideal for optical surveillance of fields, which can be used to improve agriculture around the world. We also discuss potential advances in synergetic use of RS and already established imaging and molecular techniques. This combinatorial approach can be used to reduce associated time and cost, as well as enhance the accuracy of diagnostics of biotic and abiotic stresses.

scholarly journals Application of µ-Raman spectroscopy to the study of the corrosion products of archaeological coins

ACTA IMEKO ◽  
2021 ◽  
Vol 10 (1) ◽  
pp. 234
Author(s):  
Tilde De Caro ◽  
Emma Angelini ◽  
Leila Es Sebar
Keyword(s):  
Raman Spectroscopy ◽  
Analytical Techniques ◽  
Corrosion Products ◽  
Lead Chloride ◽  
X Ray Diffraction ◽  
X Ray ◽  
Micro Raman Spectroscopy ◽  
Experimental Findings ◽  
Non Destructive ◽  
Excavation Site

<p>In this paper, a study of the corrosion products formed on archaeological bronze artefacts excavated in Tharros (Sardinia, Italy) is presented. The investigation was carried out by means of the combination of different analytical techniques, including optical microscopy, micro-Raman spectroscopy (µ-RS), scanning electron microscopy coupled with energy dispersive X-ray spectroscopy and X-ray diffraction. The artefacts under study are three bronze coins from the Phoenician–Punic period that are deeply corroded due to the chloride-rich soil of the Tharros excavation site. µ-Raman spectroscopy was chosen to investigate the corroded surfaces of the artefacts because it is a non-destructive technique, it has high spatial resolution, and it makes it possible to discriminate between polymorphs and correlate colour and chemical composition. Through µ-RS, it was possible to identify different mineralogical phases and different polymorphs, such as cuprite (Cu<sub>2</sub>O), copper trihydroxychloride [Cu<sub>2</sub>Cl(OH)<sub>3</sub>] polymorphs, hydroxy lead chloride laurionite [PbCl(OH)] and calcium carbonate polymorph aragonite. The experimental findings highlight that micro-Raman spectroscopy can be used to provide further knowledge regarding the environmental factors that may cause the degradation of archaeological bronzes in soil.</p>

scholarly journals Recent Advancements in Receptor Layer Engineering for Applications in SPR-Based Immunodiagnostics

Sensors ◽  
2021 ◽  
Vol 21 (11) ◽  
pp. 3781
Author(s):  
Marcin Drozd ◽  
Sylwia Karoń ◽  
Elżbieta Malinowska
Keyword(s):  
Medical Diagnostics ◽  
Label Free ◽  
Protein Receptors ◽  
Sensitivity And Selectivity ◽  
Analytical Reliability ◽  
Detection Of Biomarkers ◽  
Detection Strategies ◽  
Receptor Layer

The rapid progress in the development of surface plasmon resonance-based immunosensing platforms offers wide application possibilities in medical diagnostics as a label-free alternative to enzyme immunoassays. The early diagnosis of diseases or metabolic changes through the detection of biomarkers in body fluids requires methods characterized by a very good sensitivity and selectivity. In the case of the SPR technique, as well as other surface-sensitive detection strategies, the quality of the transducer-immunoreceptor interphase is crucial for maintaining the analytical reliability of an assay. In this work, an overview of general approaches to the design of functional SPR-immunoassays is presented. It covers both immunosensors, the design of which utilizes well-known and often commercially available substrates, as well as the latest solutions developed in-house. Various approaches employing chemical and passive binding, affinity-based antibody immobilization, and the introduction of nanomaterial-based surfaces are discussed. The essence of their influence on the improvement of the main analytical parameters of a given immunosensor is explained. Particular attention is paid to solutions compatible with the latest trends in the development of label-free immunosensors, such as platforms dedicated to real-time monitoring in a quasi-continuous mode, the use of in situ-generated receptor layers (elimination of the regeneration step), and biosensors using recombinant and labelled protein receptors.

scholarly journals Through-container quantitative analysis of hand sanitizers using spatially offset Raman spectroscopy

2021 ◽  
Vol 4 (1) ◽  
Author(s):  
Nirzari Gupta ◽  
Jason D. Rodriguez ◽  
Huzeyfe Yilmaz
Keyword(s):  
Raman Spectroscopy ◽  
Screening Method ◽  
High Volume ◽  
Support Vector ◽  
Hand Sanitizer ◽  
The Us ◽  
Container Screening ◽  
Non Destructive ◽  
Medical Countermeasures

AbstractThe COVID-19 pandemic created an increased demand for hygiene supplies such as hand sanitizers. In response, a large number of new domestic or imported hand sanitizer products entered the US market. Some of these products were later found to be out of specification. Here, to quickly assess the quality of the hand sanitizer products, a quantitative, through-container screening method was developed for rapid and non-destructive screening. Using spatially offset Raman spectroscopy (SORS) and support vector regression (SVR), active ingredients (e.g., type of alcohol) of 173 commercial and in-house products were identified and quantified regardless of the container material or opacity. Alcohol content in hand sanitizer formulations were predicted with high accuracy $$(R^2\, > \,0.98)$$ ( R 2 > 0.98 ) using SVR and $$94\%$$ 94 % of the substandard test samples were identified. In sum, a SORS-SVR method was developed and used for testing medical countermeasures used against COVID-19, demonstrating a potential for high-volume testing during public health threats.

scholarly journals Non-destructive analysis of drug distribution in nonwovens as drug-delivery systems for biomedical applications

2021 ◽  
Vol 7 (2) ◽  
pp. 504-506
Author(s):  
Thomas Reske ◽  
Katharina Wulf ◽  
Sabine Illner ◽  
Thomas Eickner ◽  
Niels Grabow ◽  
...  
Keyword(s):  
Raman Spectroscopy ◽  
Active Ingredient ◽  
Biomedical Applications ◽  
Drug Distribution ◽  
Quantitative Studies ◽  
Destructive Analysis ◽  
Special Sample Preparation ◽  
Group 2 ◽  
Non Destructive

Abstract Analysis of the active ingredient distribution of medical devices is typically performed using Raman spectroscopy, a method that is fast and inexpensive [1]. In addition, it offers the advantage of non-destructive analysis without the need for special sample preparation. Assuming that all components are Raman-active and present in sufficient quantities, their distribution can be well represented. The drug distribution in dexamethasone-loaded polymer nonwovens was investigated in order to draw conclusions on the quality of the fleece batches and to make predictions for the release behavior. In the present study, dexamethasone (DMS), a glucocorticoid was used as the active ingredient. Qualitative and quantitative studies of the content of DMS in polymer films by means of Raman spectroscopy have already been carried out in the working group [2]. A representative square section was examined to describe the distribution of active ingredients. The required number of measurement points (spectra) was determined earlier [2].

Forensic investigation of arson residue by infrared and Raman spectroscopy: From conventional to non-destructive techniques

2020 ◽  
Vol 60 (3) ◽  
pp. 206-215 ◽  
Author(s):  
Vijay Kumar Yadav ◽  
Kriti Nigam ◽  
Ankit Srivastava
Keyword(s):  
Raman Spectroscopy ◽  
Vibrational Spectroscopy ◽  
Analytical Techniques ◽  
Chemical Degradation ◽  
Spectroscopic Techniques ◽  
Original Material ◽  
Infrared And Raman Spectroscopy ◽  
Ignitable Liquids ◽  
Fire Debris ◽  
Non Destructive

Arson can result in highly challenging and complicated crime scenes. Much physical evidence undergoes chemical degradation because of the destructive nature of fire, while accelerants either completely burn or evaporate, and may be present in traces within any of the decomposed materials. To identify the original material and the accelerant involved, it is necessary to use advanced analytical techniques. Gas chromatography, with different detectors, is one of the most frequently used instruments in fire debris and accelerant analysis. Among other instruments, capillary electrophoresis and laser-induced thermal desorption Fourier transform mass spectrometry are two major contributors. Vibrational spectroscopy, including infrared absorption and Raman scattering, is one of the major non-destructive tools for the analysis of evidence because of its advantages over other spectroscopic techniques. Most studies involving vibrational spectroscopy (i.e. infrared and Raman spectroscopy) have focused on the identification of commonly found household materials, while very few studies have considered the identification of ignitable liquids. This article reviews studies based on an analysis of fire debris and accelerants by vibrational spectroscopic techniques and considers the limitations and future perspectives of arson investigations in forensic science.

PENGEMBANGAN MEDIA PEMBELAJARAN INTERAKTIF TEKNIK PEMESINAN BUBUT

2020 ◽  
Vol 6 (2) ◽  
pp. 169
Author(s):  
Philip Nababan ◽  
Efendi Napitupulu ◽  
R Mursid
Keyword(s):  
Student Learning ◽  
Learning Outcomes ◽  
Interactive Learning ◽  
Descriptive Analysis ◽  
Student Learning Outcomes ◽  
Field Trials ◽  
Student Responses ◽  
Analysis Techniques ◽  
Qualitative Descriptive

Abstrak: Penelitian ini bertujuan untuk: (1) Mengetahui tanggapan siswa terhadap kualitas media pembelajaran interaktif pada pembelajaran Teknik Pemesinan Bubut. (2) Mengetahui keefektifan media pembelajaran interaktif pada pembelajaran Teknik Pemesinan Bubut pada siswa program keahlian Teknik Pemesinan. Jenis penelitian ini adalah penelitian pengembangan. Data tentang kualitas produk pengembangan ini dikumpulkan dengan angket dan dianalisis dengan teknik analisis deskriptif kualiatatif. Hasil penelitian menunjukkan bahwa; (1) uji ahli materi pelajaran Teknik Pemesinan Bubut berada pada kualifikasi sangat baik (88,92%), (2) uji ahli desain pembelajaran berada pada kualifikasi sangat baik (85,21%), (3) uji ahli rekayasa perangkat lunak berada pada kualifikasi sangat baik (84,03%), (4) uji coba perorangan berada pada kualifikasi sangat baik (88,75%), (5) uji coba kelompok kecil berada pada kualifikasi sangat baik (91,35%) dan (5) uji coba lapangan berada pada kualifikasi sangat baik (88,31%). Hasil pengujian hipotesis membuktikan bahwa terdapat perbedaan antara hasil belajar siswa yang menggunakan media pembelajaran interaktif  dengan hasil belajar siswa yang menggunakan buku teks. Hal ini ditunjukkan dengan hasil pengolahan data diperoleh  thitung sebesar 4,68 dan ttabel sebesar 1,67 pada taraf kepercayaan 95 persen. Maka diperoleh bahwa thitung> ttabel. Disimpulkan bahwa  hasil belajar siswa yang menggunakan media pembelajaran interaktif dengan efektifitas sebesar 72,77 %. lebih tinggi dari hasil belajar siswa yang diajar dengan pembelajaran menggunakan buku teks dengan efektifitas sebesar 62,13%. Kata Kunci: media pembelajaran interaktif, teknik pemesinan bubut Abstract: This study aims to: (1) Determine student responses to the quality of interactive learning media on learning Lathe Machining Techniques. (2) Knowing the effectiveness of interactive learning media on learning of Machining Lathe in students of Machining Engineering expertise program. This type of research is development research. Data about the quality of this development product was collected by a questionnaire and analyzed by qualitative descriptive analysis techniques. The results showed that; (1) Lathe machining engineering subject matter expert test is in very good qualification (88.92%), (2) learning design expert test is in very good qualification (85.21%), (3) software engineering expert test is in in very good qualifications (84.03%), (4) individual trials were in very good qualifications (88.75%), (5) small group trials were in very good qualifications (91.35%) and (5 ) field trials are in very good qualifications (88.31%). Hypothesis testing results prove that there are differences between student learning outcomes using interactive learning media with student learning outcomes using textbooks. This is indicated by the results of data processing obtained by tcount of 4.68 and ttable of 1.67 at a confidence level of 95 percent. Then it is obtained that tcount> ttable. It was concluded that student learning outcomes using interactive learning media with an effectiveness of 72.77%. higher than student learning outcomes taught by learning to use textbooks with an effectiveness of 62.13%. Keywords: interactive learning media, lathe machining techniques

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