A New Coumarin-Acridone Compound as a Fluorescence Probe for Fe3+ and Its Application in Living Cells and Zebrafish

A new coumarin-acridone fluorescent probe S was designed and synthesized, and the structure was confirmed with 1H/13C NMR spectrometry, single-crystal X-ray diffraction, and high-resolution mass spectrometry. This probe has high sensitivity and selectivity for Fe3+ over other testing metal ions at 420 or 436 nm in acetonitrile–MOPS (3-Morpholinopropanesulfonic Acid) buffer solution (20.0 μM, pH = 6.9, 8:2 (v/v)). Under physiological conditions, the probe displayed satisfying time stability with a detection limit of 1.77 µM. In addition, probe S was successfully used to detect intracellular iron changes through a fluorescence-off mode, and the imaging results of cells and zebrafish confirmed their low cytotoxicity and satisfactory cell membrane permeability, as well as their potential biological applications.

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A highly selective and sensitive fluorescent probe for lactate dehydrogenase based on ultrabright adenosine monophosphate capped gold nanoclusters

RSC Advances ◽

10.1039/c7ra00158d ◽

2017 ◽

Vol 7 (22) ◽

pp. 13438-13443 ◽

Cited By ~ 3

Author(s):

Jiao Liu ◽

Hong-Wei Li ◽

Yuqing Wu

Keyword(s):

Lactate Dehydrogenase ◽

Detection Limit ◽

Fluorescent Probe ◽

Fluorescence Probe ◽

Gold Nanoclusters ◽

High Sensitivity ◽

Adenosine Monophosphate ◽

Low Detection Limit ◽

Sensitivity And Selectivity

The ultrabright AuNCs@AMP are used as fluorescence probe to detect lactate dehydrogenase (LDH) with high sensitivity and selectivity, showing an extremely low detection limit of 0.2 nM (26 pg μL−1, 0.8 U L−1).

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Dual-Channel Probe of Carbon Dots Cooperating with Lanthanide Complex Employed for Simultaneously Distinguishing and Sequentially Detecting Tetracycline and Oxytetracycline

Nanomaterials ◽

10.3390/nano12010128 ◽

2021 ◽

Vol 12 (1) ◽

pp. 128

Author(s):

Lei Jia ◽

Zhitao Xu ◽

Rujie Chen ◽

Xiangzhen Chen ◽

Jun Xu

Keyword(s):

Antimicrobial Agents ◽

Fluorescence Probe ◽

Color Change ◽

Logic Gate ◽

Lanthanide Complex ◽

High Sensitivity ◽

Inner Filter Effect ◽

Antenna Effect ◽

Buffer Solution ◽

Dual Channel

Tetracycline (TC) and oxytetracycline (OTC) are the most widely used broad-spectrum antimicrobial agents in tetracycline drugs, and their structures and properties are very similar, so it is a great challenge to distinguish and detect these two antibiotics with a single probe at the same time. Herein, a dual-channel fluorescence probe (SiCDs@mMIPs-cit-Eu) was developed by integrating two independent reaction sites with SiCDs-doped mesoporous silica molecular imprinting group and europium complex group into a nanomaterial. The synergistic influence of inner filter effect and “antenna effect” can be guaranteed to solve the distinction between TC and OTC. Moreover, this novel strategy can also sequentially detect TC and OTC in buffer solution and real samples with high sensitivity and selectivity. This method revealed good responses to TC and OTC ranging from 0 to 5.5 μM with a detection limit of 5 and 16 nM, respectively. Combined with the smartphone color-scanning application, the portable and cheap paper-based sensor was designed to realize the multi-color visual on-site detection of TC and OTC. In addition, the logic gate device was constructed according to the fluorescence color change of the probe for TC and OTC, which provided the application possibility for the intelligent detection of the probe.

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Synthesis and Application of p-Carboxyarsenazo for Spectrophotometric Determination of Cu(II) in Environment Water

Advanced Materials Research ◽

10.4028/www.scientific.net/amr.830.298 ◽

2013 ◽

Vol 830 ◽

pp. 298-301 ◽

Cited By ~ 1

Author(s):

Lin Gao ◽

Yan Hua Li ◽

Sheng Jie Chen ◽

Fang Chen ◽

Yuan Fang Zhang ◽

...

Keyword(s):

Standard Deviation ◽

Water Samples ◽

Absorption Maximum ◽

High Sensitivity ◽

Molar Absorptivity ◽

Buffer Solution ◽

Relative Standard ◽

Sensitivity And Selectivity ◽

Color Reagent

p-Carboxyarsenazo (CASA) color reagent was synthesized by simple diazotization reaction, and the chromomeric reaction between CASA with Cu(Ⅱ) has been studied by spectrophotometry. In pH=4.6 NaAc-HAc buffer solution, Cu(Ⅱ) reacted with CASA to form a red complex and exhibits an absorption maximum at 614 nm, Beer’s law is obeyed for 0-160 μg/L of copper in solution, the apparent molar absorptivity obtained is 1.57×104 L/(mol.cm), and the relative standard deviation (R.S.D.) is 1.6%. The proposed method exhibited high sensitivity and selectivity for Cu(Ⅱ). The results obtained by this method in the determination of environment water samples were accurate, sensitive and repeatable

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Highly selective and sensitive fluorescence probe based on thymine-modified carbon dots for Hg2+ and l-cysteine detection

RSC Advances ◽

10.1039/c5ra18432k ◽

2015 ◽

Vol 5 (108) ◽

pp. 89121-89127 ◽

Cited By ~ 18

Author(s):

Hui Xu ◽

Shanshan Huang ◽

Caiyun Liao ◽

Yang Li ◽

Baozhan Zheng ◽

...

Keyword(s):

Carbon Dots ◽

Fluorescence Probe ◽

High Sensitivity ◽

Sensitivity And Selectivity

The as-prepared thymine-modified carbon dots were applied to as a sensor for detecting Hg2+ and l-cysteine with high sensitivity and selectivity.

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CdSe Quantum Dots Decorated by 3-Aminobenzeneboronic Acid as Fluorescence Probe for Glucose

Advanced Materials Research ◽

10.4028/www.scientific.net/amr.306-307.1338 ◽

2011 ◽

Vol 306-307 ◽

pp. 1338-1341

Author(s):

Shao Wei Wang ◽

Xia Ona Zhang ◽

Jing Hua Yu

Keyword(s):

Quantum Dots ◽

Fluorescence Probe ◽

High Sensitivity ◽

Cdse Quantum Dots ◽

Optimal Conditions ◽

Cdse Qds ◽

Relative Standard ◽

Sensitivity And Selectivity

A simple route to the production of high-quality CdSe quantum dots (QDs) is presented. The characterization of CdSe QDs was measured by Zetasizer Nano ZS, ultraviolet-visible spectoscopy and fluorospectrophotometer. The CdSe QDs were decorated by 3-Aminobenzeneboronic acid to obtain good fluorescent characteristic. Under the optimal conditions, the decreased normalized absorbance was linear to the concentration of glucose from 1.0×10−7 to 2.9×10−6 mol·L-1with a detection limit of 5.0´10-8mol·L-1. The relative standard deviation (RSD) for the determination of 1.0×10-7mol·L-1was 1.4 % by 11 replicate determination. In this paper, the fluorescent behavior of glucose with the decorated CdSe QDs was studied, and it is found that the system has a high sensitivity and selectivity for analysis of glucose.

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Development of a bioanalytical method for an antisense therapeutic using high-resolution mass spectrometry

Bioanalysis ◽

10.4155/bio-2020-0225 ◽

2020 ◽

Vol 12 (24) ◽

pp. 1739-1756

Author(s):

Yuchen Sun ◽

Shin-ichiro Nitta ◽

Kosuke Saito ◽

Ryuta Hosogai ◽

Keiko Nakai ◽

...

Keyword(s):

Mass Spectrometry ◽

High Resolution ◽

High Resolution Mass Spectrometry ◽

High Sensitivity ◽

Rat Plasma ◽

Bioanalytical Method ◽

High Resolution Mass ◽

Sensitivity And Selectivity ◽

Carry Over ◽

Resolution Mass

Background: Ion-pairing reverse-phase LC coupled with high-resolution mass spectrometry (IP-LC/HRMS) has gained attention in oligonucleotide therapeutic bioanalyses owing to its high sensitivity and selectivity. However, optimization and validation of IP-LC/HRMS-based methods are rare. The objective of this study is the development of a sensitive and reproducible IP-LC/HRMS-based bioanalytical method using clinically approved mipomersen as a model for antisense oligonucleotides. Materials & methods/results: Mipomersen was extracted from rat plasma using Clarity OTX SPE and quantified by IP-LC/HRMS. The calibration range was 0.5–250.0 ng/ml. The developed method met the general regulatory criteria for accuracy, precision, carry-over, selectivity, matrix effect and dilution integrity. Conclusion: A highly sensitive and reliable method for mipomersen measurement with potential antisense oligonucleotide bioanalysis applications has been developed.

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Reversible Fluorescent Turn-on Sensors for Fe3+ based on a Receptor Composed of Tri-oxygen Atoms of Amide Groups in Water

Open Chemistry ◽

10.1515/chem-2018-0140 ◽

2018 ◽

Vol 16 (1) ◽

pp. 1268-1274 ◽

Cited By ~ 3

Author(s):

Jiaoliang Wang ◽

Liping Long ◽

Guing Xiao ◽

Fang Fang

Keyword(s):

Limit Of Detection ◽

Pure Water ◽

High Sensitivity ◽

Fluorescent Sensors ◽

Cell Membrane Permeability ◽

The Novel ◽

Turn On ◽

Low Cytotoxicity ◽

Fluorescence Turn On ◽

Oxygen Atoms

AbstractCompounds Rh6G-1 and RhB-2 with a novel receptor composed of tri-oxygen atoms of amide groups were designed and synthesized as new reversible fluorescent sensors for Fe3+. The prominent features of the novel sensor Rh6G-1 include a large fluorescence turn-on response in essentially pure water at room temperature, high sensitivity, high selectivity, a limit of detection, cell membrane permeability, and low cytotoxicity. These desirable attributes enable us to successfully employ the new sensor Rh6G-1 for Fe3+ bioimaging in living cells.

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Schottky diodes based on 2D materials for environmental gas monitoring: a review on emerging trends, recent developments and future perspectives

Journal of Materials Chemistry C ◽

10.1039/d0tc04840b ◽

2021 ◽

Author(s):

Minu Mathew ◽

Chandra Sekhar Rout

Keyword(s):

Gas Sensing ◽

2D Materials ◽

Schottky Diodes ◽

High Sensitivity ◽

Future Perspectives ◽

Working Temperature ◽

Emerging Trends ◽

Gas Sensing Properties ◽

Recent Developments ◽

Sensitivity And Selectivity

This review details the fundamentals, working principles and recent developments of Schottky junctions based on 2D materials to emphasize their improved gas sensing properties including low working temperature, high sensitivity, and selectivity.

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