scholarly journals Preservative Effect of Aqueous and Ethanolic Extracts of the Macroalga Bifurcaria bifurcata on the Quality of Chilled Hake (Merluccius merluccius)

Molecules ◽  
2021 ◽  
Vol 26 (12) ◽  
pp. 3774
Author(s):  
José M. Miranda ◽  
Bin Zhang ◽  
Jorge Barros-Velázquez ◽  
Santiago P. Aubourg
Keyword(s):  
Ph Value ◽  
Storage Period ◽  
Ethanolic Extract ◽  
Thiobarbituric Acid ◽  
Secondary Compounds ◽  
Inhibitory Effect ◽  
Hydrolysis Rate ◽  
Merluccius Merluccius ◽  
Bifurcaria Bifurcata ◽  
Ethanolic Extracts

This work addressed the preservative behaviour of different icing media containing extracts from the alga Bifurcaria bifurcata. A comparative study of the antimicrobial and antioxidant effects of aqueous and ethanolic extracts of this macroalga was carried out. Whole hake (Merluccius merluccius) pieces were stored in ice containing either kind of extract and analysed for quality changes throughout a 13-day storage period. A progressive loss of microbial and biochemical quality was detected in all batches as chilling time increased. A significant inhibitory effect (p < 0.05) on microbial activity could be observed as a result of including the aqueous (lowering of psychrotrophic and lipolytic counts and pH value) and ethanolic (lowering of psychrotrophic and lipolytic counts) extracts. Additionally, both kinds of extract led to a substantial inhibition (p < 0.05) in the lipid hydrolysis rate (formation of free fatty acids), greater in the case of the batch containing ethanolic extract. Concerning lipid oxidation, a similar inhibitory effect (p < 0.05) on the formation of secondary compounds (thiobarbituric acid substances) was noticed in fish specimens corresponding to both alga extracts; however, more (p < 0.05) peroxide formation was detected in fish corresponding to the ethanolic extract batch. A preservative effect can be concluded for both kinds of extract; this effect agrees with previous studies reporting the presence of hydrophilic and lipophilic bioactive compounds in B. bifurcata.

Evaluation of Phytochemicals and Pharmacological Activities of Ben-Cha-Lo-Ka-Wi-Chian Remedy

2019 ◽  
Vol 891 ◽  
pp. 52-59
Author(s):  
Chanai Noysang ◽  
Teerarat Pummarin
Keyword(s):  
Antioxidant Activity ◽  
Minimum Inhibitory Concentration ◽  
Inhibitory Concentration ◽  
Propionibacterium Acnes ◽  
Ethanolic Extract ◽  
Inhibition Zone ◽  
Inhibitory Effect ◽  
Pharmacological Activities ◽  
Ethanolic Extracts ◽  
Reaction Test

Benchalokawichian (BLW) remedy is a Thai traditional medicine that has been notified in the List of Medicine Products of the National List of Essential Drugs A.D. 2006 and has long been used as an antipyretic. The phytochemicals of the 70% and 95% ethanolic extracts of were studied by color reaction test and HPTLC analysis. The BLW remedy showed a highest amounts of 70% ethanolic extractives (ca. 3.99 ± 0.60% of dry material weight). The several ethanolic extracts showed similar qualitative phytochemicals. The major of phytochemicals identified in these extracts were alkaloids, flavonoids, tannins, and polyphenols. The antioxidant activity was assessed using the 2,2-diphenyl-1-picrylhydrazyl assay. The analysed 70% ethanolic extract showed a highest antioxidant activity at IC50= 526.09 µg/ml and both extracts not showed tyrosinase inhibitory effect. The 95% ethanolic extract showed the greatest anti-propionibacterium acnes activity with inhibition zone of 10.10 ± 0.45 mm and minimum inhibitory concentration (MIC) was 5.05 mg/ml

Preliminary Phytochemicals and Pharmacologic Activities Assessment of White and Pink Nelumbo nucifera Gaertn. Flowers

2019 ◽  
Vol 891 ◽  
pp. 41-51
Author(s):  
Chanai Noysang ◽  
Nutsuda Boonmatit
Keyword(s):  
Antioxidant Activity ◽  
Ethanolic Extract ◽  
Inhibitory Effect ◽  
White Flower ◽  
Nelumbo Nucifera ◽  
Color Reaction ◽  
Natural Source ◽  
Pink Flower ◽  
Ethanolic Extracts ◽  
Reaction Test

The phytochemicals of the ethanolic extracts of petals and stamens of lotus flowers from several lotus cultivars belonging to the Nelumbo nucifera Gaertn. (namely 'lotus white flower' and 'lotus pink flower') were studied by color reaction test and HPTLC analysis. The lotus pink flower stamens showed a highest amount of ethanolic extractives (ca. 18.33±2.14% of dry material weight). The several the ethanolic extracts of N. nucifera petals and stamens showed similar qualitative phytochemicals. The crude extracts revealed the presence of flavonoids, terpenoinds, tannins and polyphenols. The antioxidant activity was assessed using the 2,2-diphenyl-1-picrylhydrazyl assay. The analyzed lotus pink flower stamens ethanolic extract showed a highest antioxidant activity at IC50 = 0.61±0.16 μg/ml and lotus pink flower petals ethanolic extract showed a highest tyrosinase inhibitory effect at IC50 = 2.25±0.21 μg/ml. N. nucifera flowers could be provide a potential natural source of phytochemicals and could be beneficial to the cosmetic properties.

Antimicrobial and Antioxidant Activities of Deer Blood Hydrolysate on Fresh Beef during Refrigerated Storage

2013 ◽  
Vol 634-638 ◽  
pp. 1417-1422
Author(s):  
Shuai Wang ◽  
Feng Bo Xu ◽  
Li Fu Zhu ◽  
Yan Ze Yu ◽  
Chun Zi Na
Keyword(s):  
Lipid Oxidation ◽  
Ph Value ◽  
Antioxidant Activities ◽  
Ground Beef ◽  
Thiobarbituric Acid ◽  
Natural Antioxidants ◽  
Inhibitory Effect ◽  
Plate Count ◽  
Refrigerated Storage ◽  
Color Deterioration

The aim of this work was to determine the inhibitory effect of deer blood hydrolysates addition on lipid oxidation, color deterioration and microbial contamination of ground beef during during 9 days of refrigerated storage. The pH value, total plate count, thiobarbituric acid-reactive substances value (TBARS), total volatile basic nitrogen (TVB-N) and color were also determined. The results showed that the treatments added hydrolysates had significant inhibitory effects on lipid oxidation and kept the red color of ground beef in 9 days, when compared with controls (P < 0.05). Furthermore, the inhibitory effect enhanced as the adding level of the hydrolysates increased (P < 0.05). These results suggest that deer blood hydrolysates are good source of natural antioxidants.

scholarly journals Semirefined Carrageenan (SRC) Film Incorporated with α-Tocopherol and Persicaria minor for Meat Patties Application

2019 ◽  
Vol 19 (4) ◽  
pp. 1008
Author(s):  
Khadijah Husna Abd Hamid ◽  
Wan Amnin Wan Yahaya ◽  
Neenasha Bebe Mohd Nor ◽  
Alyaa Syahierra Ghazali ◽  
Siti Kholijah Abdul Mudalip ◽  
...  
Keyword(s):  
Ph Value ◽  
Storage Period ◽  
Food Product ◽  
Thiobarbituric Acid ◽  
Total Phenolic ◽  
Thiobarbituric Acid Reactive Substance ◽  
Ph Values ◽  
Color Development ◽  
Tbars Assay ◽  
Persicaria Minor

Semirefined carrageenan (SRC) plasticized with glycerol (G) and incorporated with antioxidants of 0.4% (v/v) of α-tocopherol and Persicaria minor (PM) extract was successfully developed. The objective of this study is to analyze the antioxidant effect of active packaging films from semirefined carrageenan incorporated with α-tocopherol and Persicaria minor on meat patties. Total phenolic content and antioxidant activity of α-tocopherol and PM extract were measured. The effects of α-tocopherol and PM extract incorporated with SRC-based films on meat patties were evaluated using thiobarbituric acid reactive substance (TBARS) assay, metmyoglobin assay, and pH value for 14 days of storage. The films with 0.4% (v/v) of α-tocopherol and PM extract exhibited a lower lipid oxidation in meat patties compared with that of control (SRC film only, p < 0.05). Also, a brown color development of the meat patties of less than 50% was observed at the end of the 14-day storage. Meanwhile, the pH values for all samples decreased throughout the storage period with the SRC+G+α-tocopherol film showed the highest pH value. Hence, the formulation of SRC film with α-tocopherol or PM extract could be used as an alternative packaging for extending the shelf life of food product with high fat content.

Comparison of Ozone and Chlorine in Low Concentrations as Sanitizing Agents of Chicken Carcasses in the Water Immersion Chiller

2012 ◽  
Vol 75 (6) ◽  
pp. 1139-1143 ◽  
Author(s):  
MARCO ANTONIO TRINDADE ◽  
MARTA MITSUI KUSHIDA ◽  
NILDA D. MONTES VILLANUEVA ◽  
DAVID UENAKA dos SANTOS PEREIRA ◽  
CELSO EDUARDO LINS de OLIVEIRA
Keyword(s):  
Lipid Oxidation ◽  
Ph Value ◽  
Water Immersion ◽  
Storage Period ◽  
Thiobarbituric Acid ◽  
Total Coliform ◽  
Ozone Treatment ◽  
Thiobarbituric Acid Reactive Substances ◽  
Hedonic Scale ◽  
The Stability

The aim of this study was to investigate the effects of the use of chlorine or ozone as sanitizing agents in the water of chicken immersion chilling, using the residual levels usually applied in Brazil (1.5 ppm), comparing the effects of these treatments on the microbiological, physicochemical, and sensory characteristics of carcasses. Chicken carcasses were chilled in water (4°C) with similar residual levels of ozone and chlorine until reaching temperatures below 7°C (around 45 min). The stability of carcasses was assessed during 15 days of storage at 2 ± 1°C. Microbiological, surface color (L*, a*, b* parameters), pH value, lipid oxidation (thiobarbituric acid reactive substances index), and sensory evaluation (on a 9-point hedonic scale for odor and appearance) analyses were carried out. The presence of Salmonella was not detected, coagulase-positive staphylococci counts were below 102 CFU/ml of rinse fluid, and Escherichia coli and total coliform counts were below 105 CFU/ml of rinse fluid until the end of the storage period for both treatments. Psychrotrophic microorganism counts did not differ (P &gt; 0.05) between chlorine and ozone treatments, and both values were near 109 CFU/ml of rinse fluid after 15 days at 4 ± 1°C. pH values did not differ between treatments (P &gt;0.05) or during the storage period (P &gt; 0.05). In addition, neither chlorine nor ozone treatment showed differences (P &gt; 0.05) in the lipid oxidation of carcasses; however, the thiobarbituric acid reactive substances index of both treatments increased (P ≤ 0.05) during the storage period, reaching values of approximately 0.68 mg of malonaldehyde per kg. Samples from both treatments did not differ (P &gt; 0.05) in their acceptance scores for odor and overall appearance, but in the evaluation of color, ozone showed an acceptance score significantly higher (P ≤ 0.05) than that for the chlorine treatment. In general, under the conditions tested, ozone showed results similar to the results for chlorine in the disinfection of chicken carcasses in the immersion chilling, which may indicate its use as a substitute for chlorine in poultry slaughterhouses.

scholarly journals Quality of Refrigerated Tilapia (Oreochromis niloticus) Slices under Vacuum and Modified Atmosphere Packaging

2020 ◽  
Vol 15 (3) ◽  
pp. 99
Author(s):  
Md. Abdul Karim ◽  
Masudur Rahman ◽  
Esmout Jahan Alice ◽  
Md. Amanullah ◽  
Md. Akhtar Hossain ◽  
...  
Keyword(s):  
Shelf Life ◽  
Oreochromis Niloticus ◽  
Ph Value ◽  
Control Sample ◽  
Modified Atmosphere Packaging ◽  
Storage Period ◽  
Thiobarbituric Acid ◽  
Life Extension ◽  
Modified Atmosphere ◽  
Acceptable Limit

In order to develop a proper packaging system for sliced tilapia fish (Oreochromis niloticus), the biochemical and microbiological qualities under control (unsealed package), vacuum package and modified atmosphere packaging with 50% CO2/50% N2 (MAP-1)  and 50% CO2/50% O2 (MAP-2)  were evaluated every three days during 18 days of chilled storage (4±1°C). The pH value was not significantly different (p > 0.05) by treatments until day 9, but significantly lower values (p < 0.05) were observed on day 12 of the storage in all treated samples compared to the control. The total volatile base nitrogen (TVB-N) value progressively increased, but not significantly different (p > 0.05) in all treatments during the entire storage period. The thiobarbituric acid reactive substances (TBARS) amounts were significantly lower (p < 0.05) on day 12 in the vacuum and MAP-1 samples compared to the control sample, and were significantly higher (p < 0.05) on day 6, 9, and 12 of the storage period in MAP-2 samples compared to the control, vacuum and MAP-1 samples. The amounts of pH, TVB-N, and TBARS in all samples did not exceed the acceptable limit in almost the entire storage. The total viable count (TVC) progressively increased with storage time. Nevertheless, TVC values were lower (p < 0.05) on day 6, 9, and 12 of the storage periods in all treatments compared to the control. The TVCs exceeded the acceptable limit (7 log CFU/g) on days 6-9 for control, 9-12 for vacuum, day 12 for MAP-2, and 15 for MAP-1 sample during the storage period. Therefore, the MAP has shown promising results for shelf life extension that can be practiced to display the fishery products with prolonged shelf life. 

scholarly journals Effect of spice extracts in combination with packaging materials and treatments on the stability of ground buffalo meat product stored under frozen conditions

2004 ◽  
Vol 20 (1-2) ◽  
pp. 1-15 ◽  
Author(s):  
M.A. Kenawi ◽  
H.A. Abdel-Aal ◽  
S.S. Latif
Keyword(s):  
Ph Value ◽  
Meat Product ◽  
Storage Period ◽  
Thiobarbituric Acid ◽  
Natural Antioxidants ◽  
Packaging Materials ◽  
Black Cumin ◽  
Buffalo Meat ◽  
The Stability ◽  
Control Samples

Proximate analysis, expressible water(EP), water holding capacity (WHC), pH value, total acidity, free fatty acids (FFA), thiobarbituric acid values (TBA), color evaluation, and microbiological examination were determined in order to evaluate the effect of spice extracts in combination with packaging materials and treatments on the stability of ground buffalo meat product stored under frozen conditions up to six months. The samples were treated by spice extracts and packaged in two packaging materials low density polyethylene (LDPE) bags without vacuum, and laminated polyethylene/nylon bags under vacuum. The results indicated that all the samples had lost moisture during storage, and the loss was higher in samples packaged in (LDPE), also the (EP) values increased, whereas, (WHC) values decreased with time during storage for all samples. The samples indicated a reduction in the pH values and an increase in the acidity values especially for the first four months of storage and these changes were associated with an increase in the (FFA) values as a result of storage. The increment in the pH value was the lowest in the control samples and this indicated the effect of natural antioxidants to retard the formation of (FFA). The (TBA) values for the control samples were higher than those packaged under vacuum or treated by spice extracts. The storage time had a negative effect on the total bacterial counts and the coliform group for all samples. The rate of reduction was much higher in the vacuum packaged samples and the spice extracts treated samples as well especially those treated by black cumin extracts than the untreated or samples packaged without vacuum. The color measurements showed a decrease in the redness value for all samples during storage period. The rate of reduction was much higher in the control samples than the treated ones.

scholarly journals Effect of Serjania lethalis ethanolic extract on weed control

2013 ◽  
Vol 31 (2) ◽  
pp. 239-248 ◽  
Author(s):  
P.U. Grisi ◽  
S.C.J. Gualtieri ◽  
S. Anese ◽  
V.C. Pereira ◽  
M.R. Forim
Keyword(s):  
Seedling Growth ◽  
Weed Management ◽  
Plant Material ◽  
Ethanolic Extract ◽  
Inhibitory Effect ◽  
Dependent Manner ◽  
Buffer Solution ◽  
Concentration Dependent Manner ◽  
Promising Alternative ◽  
Ethanolic Extracts

The objective of this study was to evaluate the effect of the ethanolic extract of Serjania lethalis leaves and stems on the diaspore germination and seedling growth of wild poinsettia (Euphorbia heterophylla) and barnyardgrass (Echinochloa crus-galli). The crude ethanolic extract was prepared from 100 g of dry plant material dissolved in 500 ml of ethanol. The extracts were solubilized in a buffer solution containing dimethyl sulfoxide (DMSO) at concentrations of 10.0, 7.5, 5.0 and 2.5 mg mL-1. The effect of these extracts was compared with herbicide oxyfluorfen in bioassays. The ethanolic extracts of S. lethalis leaves and stems inhibited the germination and seedling growth of barnyardgrass and wild poinsettia in a concentration-dependent manner. The reduction in the root length of E. heterophylla seedlings might be attributed to the reduced elongation of metaxylem cells. The phytotoxicity of the extracts ranged according to the receptor species, and for some variables, the inhibitory effect was similar, and even superior, to that of the commercial herbicide. Thus, S. lethalis extracts might be a promising alternative for sustainable weed management.

scholarly journals Antityrosinase activity ofEuphorbia characiasextracts

PeerJ ◽  
2015 ◽  
Vol 3 ◽  
pp. e1305 ◽  
Author(s):  
Francesca Pintus ◽  
Delia Spanò ◽  
Angela Corona ◽  
Rosaria Medda
Keyword(s):  
Competitive Inhibition ◽  
Ethanolic Extract ◽  
Inhibitory Effect ◽  
Cellular Systems ◽  
Tyrosinase Activity ◽  
Dependent Manner ◽  
Mushroom Tyrosinase ◽  
Leaf Extracts ◽  
Melanin Biosynthesis ◽  
Ethanolic Extracts

Tyrosinase is a well-known key enzyme in melanin biosynthesis and its inhibitors have become increasingly important because of their potential use as hypopigmenting agents. In the present study, the anti-melanogenic effect of aqueous and ethanolic extracts fromEuphorbia characiasleaves, stems, and flowers in cell-free and cellular systems was examined. All the extracts showed inhibitory effects against mushroom tyrosinase with leaf extracts exhibiting the lowest IC50values of 24 and 97 µg/mL for aqueous and ethanolic extracts respectively. Enzyme kinetic analysis indicated that leaf aqueous extract acts as a mixed type inhibitor, while ethanolic extract shows a competitive inhibition effect on mushroom tyrosinase using L-DOPA as substrate. In addition, the inhibitory effect of leaf extracts on tyrosinase activity and melanin production was examined in murine melanoma B16F10 cells. Cellular tyrosinase activity as well as levels of melanin synthesis are reduced in a dose-dependent manner by extracts in cells treated withα-melanocyte stimulating hormone (α-MSH). The effects are comparable, and sometimes even better, than that of kojic acid, a well known tyrosinase inhibitor used for reference. All these results suggest thatE. characiascould be a great source of the natural inhibitors from tyrosinase and has the potential to be used as a whitening agent in therapeutic fields.

scholarly journals Different 4T1 Cells Migration under Caesalpinia sappan L. and Ficus septica Burm.f Ethanolic Extracts

2017 ◽  
Vol 8 (1) ◽  
pp. 21 ◽  
Author(s):  
Sari Haryanti ◽  
Retno Murwanti ◽  
Herwandhani Putri ◽  
Gagas Pradani Nur Ilmawati ◽  
Suwijiyo Pramono ◽  
...  
Keyword(s):  
Anticancer Agent ◽  
Gelatin Zymography ◽  
Ethanolic Extract ◽  
Cell Types ◽  
Inhibitory Effect ◽  
Caesalpinia Sappan ◽  
Ic50 Value ◽  
Anti Cancer ◽  
Ethanolic Extracts ◽  
4T1 Cells

Secang (C. sappan L.) and awar (F. septica Burm.f) are known of Indonesian traditional medicine that highly consumed throughout centuries in order to cure various diseases. Recently, researchers also concern about its effects as anti-cancer on various cell types. This study was conducted to understand the ethanolic extract of C. sappan L. (ECS) and F. septica Burm.f (EFS) effects on 4T1 cells migration at various concentrations. Firstly, we examine cell proliferation profile with MTT assay under treatment with the extracts and obtained the IC50 value of ECS (20 μg/mL) and EFS (15 μg/mL). Subsequent assay conducted was to examine the cells migration under low concentration resulting in the migration inhibitory effect of both EFS and ECS with different intensity.  EFS performed better migration inhibitory effect than ECS. Finally, we conducted gelatin zymography and western blot and revealed that the migration inhibitory effect of EFS may correlate to the lowering of protein expression of MMP9 and Rac-1 after 24 hours of treatment. We conclude that both extracts are potential to be developed as anticancer agent and EFS is more potent for anti-metastasis.Keywords: C. sappan L., F. septica Burm.f, 4T1, anti-migration

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