scholarly journals FORMULATION OF ANTIOXIDANT GEL FROM STANDARDIZED GREEN CINCAU (CYCLEA BARBATA L. MIERS) ETHANOLIC EXTRACT

2020 ◽  
pp. 236-240
Author(s):  
ERLINDHA GANGGA ◽  
YUNAHARA FARIDA ◽  
KARTININGSIH
Keyword(s):  
Antioxidant Activity ◽  
Microbial Contamination ◽  
Ph Value ◽  
Ethanolic Extract ◽  
Premature Aging ◽  
Phytochemical Screening ◽  
Active Ingredients ◽  
Greenish Yellow ◽  
Ic50 Value ◽  
Ethanolic Extracts

Objective: The aim of this study to develop an antioxidant gel from standardized green cincau (Cyclea barbata L. Miers) ethanolic extracts. Methods: The standardized extract of green cincau (Cyclea barbata L. Miers) ethanolic extracts were formulated as active ingredients of antioxidant gel. The dried leaf of Cyclea barbata L Miers was extracted with ethanol 70%. The extract was analyzed its phytochemical screening and antioxidant activity with DPPH methods. The extract was used as active ingredients of antioxidant gel. The gels were evaluated physically and chemically, such as organoleptic test, homogeneity, viscosity and rheology, spreadability, pH test, antioxidant activity, and analysis of microbial contamination test. Results: The results showed that all formulas were greenish-yellow and have homogeneous, the viscosity of 14267-45533 cps, spreadability of gels 1783.8–6631.5 mm2, pH value of 4.57–5.74, and analysis of microbial contamination has colonies not more than 103 colonies/g or colonies/ml. Statistical analysis used two ways ANOVA to investigate gels time and temperature effects toward gel preparations stability. There was no effect on the viscosity, spreadability, and pH tests, on the all formula with P>0.05. The antioxidant activity of the formula showed that all formulas changed after 3 mo of storage at 40 °C. Formula II had stronger antioxidant activity than the others with an IC50 value of 57.60µg/ml. Conclusion: All formulas showed fairly strong antioxidant activity because it can inhibit the activity of free radicals that could inhibit the premature aging of the skin.

UJI AKTIVITAS ANTIOKSIDAN FRAKSI N-HEKSAN DAUN AFRIKA (Vernonia amygdalina Del.) DENGAN METODE DPPH

2020 ◽  
Vol 5 (2) ◽  
pp. 250-257
Author(s):  
Nurul Fatimah ◽  
◽  
Reksi Sundu
Keyword(s):  
Oxidative Stress ◽  
Antioxidant Activity ◽  
Free Radicals ◽  
Ethanolic Extract ◽  
Ethanol Extract ◽  
Hexane Fraction ◽  
Phytochemical Screening ◽  
Vernonia Amygdalina ◽  
Tropical Africa ◽  
Ic50 Value

Free radicals and reactive species are widely believed to contribute to the development of several diseases by causing oxidative stress and eventually oxidative. Vernonia amygdalina (Astereacea) is a small shrub or tree between 1 and 5m high growing throughout tropical Africa. Plants are generally known as bitter leaves is well cultivated and is a general market for merchandise in several countries. The purpose of this study was to determine the antioxidant activity of hexane fraction from ethanol extract od Frican leaves (Vernonia amygdalina Del.). The method used in this study was the DPPH (1,1-Diphenil-2-Picrylhydrazyl) method. The result of phytochemical screening showed that ethanolic extract of African leaves contained a composition of secondary metabolites of alkaloids, flavonoids, tannins, steroids/triterpenoids and saponins. The antioxidant activity of the extract of n-hexane fraction was classified as very weak with an IC50 value of 317.98 ppm.

scholarly journals The The Phytochemical Screening, Total Phenolic Contents and Antioxidant Activities in Vitro of White Oyster Mushroom (Pleurotus Ostreatus) Preparations

2019 ◽  
Vol 7 (15) ◽  
pp. 2404-2412
Author(s):  
Santun Bhekti Rahimah ◽  
Dhiah Dianawaty Djunaedi ◽  
Arto Yuwono Soeroto ◽  
Tatang Bisri
Keyword(s):  
Antioxidant Activity ◽  
Pleurotus Ostreatus ◽  
Antioxidant Activities ◽  
Ethanolic Extract ◽  
Total Phenolic ◽  
Phytochemical Analysis ◽  
Phytochemical Screening ◽  
Ethanolic Extracts ◽  
Active Substances

BACKGROUND: The popular commercially cultivated Pleurotus ostreatus mushroom contains very high nutrients and bioactive compounds with high antioxidant activity. The ethanolic extract seems to be the most active in preparation. AIM: This study has an aim to compare the phytochemical analysis of a fresh, dry and ethanolic extract of Pleurotus ostreatus, to measure the total phenolic content and antioxidant activities in vitro of ethanolic extracts of Pleurotus ostreatus. METHODS: The fresh plant's materials (FPM), dry plants materials (DPM), ethanolic extracts were macerated with 70% (EE70) and 96% ethanol (EE96) of Pleurotus ostreatus which were used for phytochemical analysis, and EE96 was used for antioxidant activity in vitro. The phytochemical analysis was conducted using the Dragendorf and Meyer, FeCl3 test, Salkowsky method, Lieberman method, amyl alcohol, foam test and the NaOH reagent. The total phenol test was carried out using the Follin-Ciocalteu method. The antioxidant activity was tested using the ABTS and H2O2 essay. RESULTS: The phytochemical screening showed that the flavonoid, phenolic compounds, tannin, saponin, alkaloids, and steroids were detected in the FPM, DPM, EE70 and also the EE96. The alkaloid, however, was not identified by the Meyer Reagent in the FPM and DPM. The DPM and EE70 seemed to have the highest amount of saponin based on the foam that was formed. Meanwhile, steroids and flavonoids were detected at a higher level in the EE96, based on the strength of visible colour. However, triterpenoid and quinones could not be identified. In the total phenol test, there was an amount of 6.67 μg phenol in a 1 mg extract sample which was equivalent to 1 mg of Gallic Acid. The EE96 has an IC50 of 108.07 μg/mL for ABTS and an IC50 reduction of 229.17 μg/mL. The process of Pleurotus ostreatus drying did not reduce the content of active substances. The polar active substances seem to be more soluble in the EE70 than the EE96. CONCLUSION: The higher the bioactive substances in the preparation, the more significant the bio-therapeutic effects. Ethanolic extract of Pleurotus ostreatus has a phenol content and a good antioxidant action.  

scholarly journals PHYTOCHEMICAL SCREENING OF ETHANOLIC EXTRACT OF WHOLE PLANT OF SIDA GLUTINOSA

2020 ◽  
pp. 65-74
Author(s):  
GUDURU RAJESWARI ◽  
SWARNA LATHA D ◽  
CHANDRA SEKHAR KB
Keyword(s):  
Antioxidant Activity ◽  
High Pressure ◽  
Total Phenolics ◽  
Ethanolic Extract ◽  
Phytochemical Screening ◽  
Isolation And Characterization ◽  
Rp Hplc ◽  
Whole Plant ◽  
Ic50 Value ◽  
Layer Chromatography

Objective: The aim of the present study is to screen for the phytochemical constituents which are of pharmacological importance present in the ethanolic extract of whole plant of Sida glutinosa (SG). Methods: The ethanolic extract of the dried whole plant of SG is subjected to preliminary phytochemical screening which showed the presence of major phytoconstituents such as phenols, flavonoids, and alkaloids. The extract was screened for its antioxidant activity by 2,2-diphenyl-1- picrylhydrazyl, hydroxyl radical, Iron (III) to Iron (II) reducing activity, and nitric oxide scavenging assay. Further, the ethanolic extract was subjected to fingerprinting technique high-pressure thin-layer chromatography (HPTLC). Reverse phase high-pressure liquid chromatography (Rp-HPLC) was performed to estimate the amount of total phenolics, flavonoids, and alkaloids quantitatively in isocratic mode. Results: Phytochemical screening of the ethanolic extract of the plant showed the presence of pharmacologically important constituents such as alkaloids, flavonoids, phenolics, and terpenoids. The study also revealed the potential antioxidant activity of the extract with IC50 value. The extract fingerprinting through HPTLC revealed the presence of various phytoconstituents. Rp-HPLC showed 0.35±0.12 μg/ml of total phenolics, 0.0013±0.05μg/ml of alkaloids, and 0.00081±0.08 μg/ml of flavonoids. Conclusion: Scientific evaluation of SG which has therapeutic significance was carried out which is an important concept for the standardization of the plant-based drug. Further, there is a need for isolation and characterization of the lead molecules their systemic evaluation for its pharmacological activities.

scholarly journals Antimicrobial and antioxidant activity of essential oil and different plant extracts of Psidium cattleianum Sabine

2016 ◽  
Vol 76 (1) ◽  
pp. 101-108 ◽  
Author(s):  
M. C. Scur ◽  
F. G. S. Pinto ◽  
J. A. Pandini ◽  
W. F. Costa ◽  
C. W. Leite ◽  
...  
Keyword(s):  
Antimicrobial Activity ◽  
Antioxidant Activity ◽  
Essential Oil ◽  
Plant Extracts ◽  
Antioxidant Activities ◽  
Biological Activities ◽  
Ethanolic Extract ◽  
Phytochemical Screening ◽  
Psidium Cattleianum ◽  
Ethanolic Extracts

Abstract The goals of the study were to determinethe antimicrobial and antioxidant activities of essential oil and plant extracts aqueous and ethanolic of Psidium cattleianum Sabine; the chemical composition of the essential oil of P. cattleianum; and the phytochemical screening of aqueous and ethanolic extracts of the same plant. Regarding the antimicrobial activity, the ethanolic extract exhibited moderate antimicrobial activity with respect to bacteria K. pneumoniae and S. epidermidis, whereas, regarding other microorganisms, it showed activity considered weak. The aqueous extract and the essential oil showed activity considered weak, although they inhibited the growth of microorganisms. About the antioxidant potential, the ethanolic and aqueous extracts exhibited a scavenging index exceeding 90%, while the essential oil didn´t show significant antioxidant activity. Regarding the phytochemical composition, the largest class of volatile compounds identified in the essential oil of P. cattleianum included the following terpenic hydrocarbons: α-copaene (22%); eucalyptol (15%), δ-cadinene (9.63%) and α-selinene (6.5%). The phytochemical screening of extracts showed the presence of tannins, flavonoids, and triterpenoids for aqueous and ethanolic extracts. The extracts and essential oils inhibit the growth of microrganisms and plant extracts showed significant antioxidant activity. Also, the phytochemical characterization of the essential oil showed the presence of compounds interest commercial, as well as extracts showed the presence of important classes and compounds with biological activities.

scholarly journals Anti-Inflammatory, Analgesic and Antipyretic Activity of Catunaregam spinosa (Thumb.) Tirveng Extracts

2019 ◽  
Vol 9 (5) ◽  
pp. 89-94
Author(s):  
Hansa Saini ◽  
Jayesh Dwivedi ◽  
Himanshu Paliwal ◽  
Udichi Kataria ◽  
Priyanka Chauhan ◽  
...  
Keyword(s):  
Ethanolic Extract ◽  
Pharmacological Properties ◽  
Phytochemical Screening ◽  
Hot Plate ◽  
Hot Plate Test ◽  
Egg Albumin ◽  
Antipyretic Activity ◽  
Ethanolic Extracts ◽  
Anti Inflammatory ◽  
Response Method

Catunaregam spinosa leaves have been ethnopharmacologically accounted for acquiring various pharmacological properties. The present study was undertaken to evaluate anti-inflammatory, analgesic and antipyretic potential of leaves of C. spinosa.The ethanolic extract was selected for this purpose based on phytochemical screening. Inflammation was inhibited at the dose of 200 mg/kg with percent inhibition of inflammation 32.06, 37.28 and 43.16 %, respectively, at 1, 3 and 5 h, while in egg albumin model % inhibition was found to be 47.81%. There was no significant analgesic activity seen in acetic acid induced writhing response method while significant effects were observed in the doses of 25 and 100 mg/kg on hot plate test. No antipyretic activity was shown by ethanolic extracts(25, 100 and 200 mg/kg) against Brewer’s yeast induced pyrexia in rats. Keywords: Catunaregam spinosa, Anti-inflammatory activity, Phytochemical screening, Ethanolic extract.

Pulicaria laciniata (Coss and Kral): Effects of Different Extraction Solvents on Phytochemical Screening and Antioxidant Activity

2021 ◽  
pp. 425-430
Author(s):  
Kamilia Bireche ◽  
Hocine Dendougui ◽  
Asma Abid ◽  
Abdeldjabbar Messaoudi ◽  
Mohamed Hadjadj
Keyword(s):  
Antioxidant Activity ◽  
Reducing Power ◽  
The Other ◽  
Phytochemical Screening ◽  
Butanol Extract ◽  
Metal Chelating ◽  
Phytochemical Investigation ◽  
Ic50 Value ◽  
Extraction Solvents ◽  
Phytochemical Constituents

This study aims to investigate phytochemical constituents of Pulicaria laciniata extracts and determine their antioxidant activity using three methods; Phosphomolybdate, Reducing Power, and Metal Chelating. The phytochemical investigation showed various secondary metabolites such as Phenols, Glycosides, Flavonoids, Alkaloids, Tannins, and Terpenoids. The N-butanol extract exhibited the highest antioxidant activity comparing with the other extract in all methods (0.51 and 0.65 mg/ml as A0.5 values of Phosphomolybdate, reducing power) and (1.65mg/ml for IC50 value of metal-chelating). In contrast, all the extracts showed week activity against the metal-chelating method.

scholarly journals Chemical Fingerprint Analysis of Phenolics of Albizia chinensis Based on Ultra-Performance LC-Electrospray Ionization-Quadrupole Time-of-Flight Mass Spectrometry and Antioxidant Activity

2011 ◽  
Vol 6 (11) ◽  
pp. 1934578X1100601 ◽  
Author(s):  
Abha Chaudhary ◽  
Pushpinder Kaur ◽  
Neeraj Kumar ◽  
Bikram Singh ◽  
Shiv Awasthi ◽  
...  
Keyword(s):  
Mass Spectrometry ◽  
Antioxidant Activity ◽  
Electrospray Ionization ◽  
Time Of Flight ◽  
Ethanolic Extract ◽  
Ultra Performance Liquid Chromatography ◽  
Reversed Phase ◽  
Flight Mass Spectrometry ◽  
Ethanolic Extracts ◽  
C Nmr

Albizia species have been shown to have anti-inflammatory and antiallergic properties. However, efficient analytical methods for identification of their active constituents are still lacking. Ultra-performance liquid chromatography coupled with electrospray ionization-quadrupole time-of-flight mass spectrometry (UPLC-ESI-QTOF-MS) was used to study the phenolic composition of the ethanolic extracts of different parts (flowers, leaves, pods and bark) of A. chinensis. In addition, the antioxidant activity of the ethanolic extracts was evaluated by the 1,1-diphenyl-2-picryl hydrazyl (DPPH) free-radical and 2,2′-azino-bis(3-ethylbenzthiazoline-6-sulfonic acid (ABTS) radical cation scavenging methods. Four compounds were isolated from the ethanolic extract of the flowers and characterized by 1H and 13C NMR spectroscopy as quercetin-3- O-rhamnoside, quercetin, quercetin-3- O-arabinofuranoside, and myricetin-3- O-rhamnoside. Separation and quantification of the phenolics was accomplished using a reversed-phase BEH C18 column with the mobile phase of methanol–water (0.05% formic acid), and detection wavelengths of 360 and 254 nm.

Evaluation of Phytochemicals and Pharmacological Activities of Ben-Cha-Lo-Ka-Wi-Chian Remedy

2019 ◽  
Vol 891 ◽  
pp. 52-59
Author(s):  
Chanai Noysang ◽  
Teerarat Pummarin
Keyword(s):  
Antioxidant Activity ◽  
Minimum Inhibitory Concentration ◽  
Inhibitory Concentration ◽  
Propionibacterium Acnes ◽  
Ethanolic Extract ◽  
Inhibition Zone ◽  
Inhibitory Effect ◽  
Pharmacological Activities ◽  
Ethanolic Extracts ◽  
Reaction Test

Benchalokawichian (BLW) remedy is a Thai traditional medicine that has been notified in the List of Medicine Products of the National List of Essential Drugs A.D. 2006 and has long been used as an antipyretic. The phytochemicals of the 70% and 95% ethanolic extracts of were studied by color reaction test and HPTLC analysis. The BLW remedy showed a highest amounts of 70% ethanolic extractives (ca. 3.99 ± 0.60% of dry material weight). The several ethanolic extracts showed similar qualitative phytochemicals. The major of phytochemicals identified in these extracts were alkaloids, flavonoids, tannins, and polyphenols. The antioxidant activity was assessed using the 2,2-diphenyl-1-picrylhydrazyl assay. The analysed 70% ethanolic extract showed a highest antioxidant activity at IC50= 526.09 µg/ml and both extracts not showed tyrosinase inhibitory effect. The 95% ethanolic extract showed the greatest anti-propionibacterium acnes activity with inhibition zone of 10.10 ± 0.45 mm and minimum inhibitory concentration (MIC) was 5.05 mg/ml

Preliminary Phytochemicals and Pharmacologic Activities Assessment of White and Pink Nelumbo nucifera Gaertn. Flowers

2019 ◽  
Vol 891 ◽  
pp. 41-51
Author(s):  
Chanai Noysang ◽  
Nutsuda Boonmatit
Keyword(s):  
Antioxidant Activity ◽  
Ethanolic Extract ◽  
Inhibitory Effect ◽  
White Flower ◽  
Nelumbo Nucifera ◽  
Color Reaction ◽  
Natural Source ◽  
Pink Flower ◽  
Ethanolic Extracts ◽  
Reaction Test

The phytochemicals of the ethanolic extracts of petals and stamens of lotus flowers from several lotus cultivars belonging to the Nelumbo nucifera Gaertn. (namely 'lotus white flower' and 'lotus pink flower') were studied by color reaction test and HPTLC analysis. The lotus pink flower stamens showed a highest amount of ethanolic extractives (ca. 18.33±2.14% of dry material weight). The several the ethanolic extracts of N. nucifera petals and stamens showed similar qualitative phytochemicals. The crude extracts revealed the presence of flavonoids, terpenoinds, tannins and polyphenols. The antioxidant activity was assessed using the 2,2-diphenyl-1-picrylhydrazyl assay. The analyzed lotus pink flower stamens ethanolic extract showed a highest antioxidant activity at IC50 = 0.61±0.16 μg/ml and lotus pink flower petals ethanolic extract showed a highest tyrosinase inhibitory effect at IC50 = 2.25±0.21 μg/ml. N. nucifera flowers could be provide a potential natural source of phytochemicals and could be beneficial to the cosmetic properties.

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