scholarly journals Bioaccessibility and Cellular Uptake of Carotenoids Extracted from Bactris gasipaes Fruit: Differences between Conventional and Ionic Liquid-Mediated Extraction

Molecules ◽  
2021 ◽  
Vol 26 (13) ◽  
pp. 3989
Author(s):  
Leonardo M. de Souza Mesquita ◽  
Daniella Carisa Murador ◽  
Bruna Vitória Neves ◽  
Anna Rafaela Cavalcante Braga ◽  
Luciana Pellegrini Pisani ◽  
...  
Keyword(s):  
Cellular Uptake ◽  
High Performance ◽  
In Vitro Digestion ◽  
Bactris Gasipaes ◽  
Purification Method ◽  
Extraction Solvent ◽  
Extraction And Purification ◽  
Feasible Alternative ◽  
Synthetic Colorants

Currently, on an industrial scale, synthetic colorants are used in many fields, as well as those extracted with conventional organic solvents (COSs), leading to several environmental issues. Therefore, we developed a sustainable extraction and purification method mediated by ionic liquids (IL), which is considered an alternative high-performance replacement for COSs. Carotenoids are natural pigments with low bioaccessibility (BCT) and bioavailability (BV) but with huge importance to health. To investigate if the BCT and cellular uptake of the carotenoids are modified by the extraction method, we conducted a comparison assay between both extraction procedures (IL vs. COS). For this, we used the Amazonian fruit Bactris gasipaes, a rich source of pro-vitamin A carotenoids, to obtain the extract, which was emulsified and subjected to an in vitro digestion model followed by the Caco-2 cell absorption assay. The bioaccessibility of carotenoids using IL was better than those using COS (33.25%, and 26.84%, respectively). The cellular uptake of the carotenoids extracted with IL was 1.4-fold higher than those extracted using COS. Thus, IL may be a feasible alternative as extraction solvent in the food industry, replacing COS, since, in this study, no IL was present in the final extract.

Study on the Extraction and Purification of Microcystins (MC-LR)

2011 ◽  
Vol 340 ◽  
pp. 318-323
Author(s):  
Wen Yi Zhang ◽  
Ning Han ◽  
Li Rong Yao ◽  
Xiao Lan Qiu ◽  
Xiao Liang Chen
Keyword(s):  
Extraction Method ◽  
Taihu Lake ◽  
High Efficiency ◽  
Extraction Efficiency ◽  
Low Cost ◽  
Solvent Concentration ◽  
Purification Method ◽  
Blue Green Algae ◽  
Extraction Solvent ◽  
Extraction And Purification

The MC-LR from the the blue-green algae of Taihu Lake was extracted, at the same time, a set of microcystins extraction method with methanol as extraction solvent and purification method with C18-SPE as purification workstations were established. The extraction solvent concentration, extraction time, extraction solvent amount, leacheate concentration and eluent concentration were used to research the extraction efficiency of MC-LR. Finally, 80% methanol was used to wash microcytins to make MC-LR high purity and the purity was over 85%. This research presented a method of low cost and high efficiency. It provided the foundation for the further research of microcytins.

scholarly journals Extraction, Characterization and Antioxidant Activity in vitro of Proteins from Semen Allii Fistulosi

Molecules ◽  
2018 ◽  
Vol 23 (12) ◽  
pp. 3235
Author(s):  
Min Zuo ◽  
Xiao-xiao Liu ◽  
Di Liu ◽  
Hang-yun Zhao ◽  
Lu-lu Xuan ◽  
...  
Keyword(s):  
Antioxidant Activity ◽  
High Performance ◽  
Orthogonal Design ◽  
Polyacrylamide Gel Electrophoresis ◽  
Sodium Dodecyl ◽  
Reducing Power ◽  
Raw Material ◽  
Extraction Solvent ◽  
Sds Page

Semen Allii Fistulosi (PSAF) is the seed of Allium fistulosum L. of the Liliaceae family. The purpose of this study was to extract, characterize, and evaluate the antioxidant activity in vitro of proteins. Using single factor and orthogonal design, the optimum conditions of extraction were determined to be as follows: extraction time 150 min, pH 8.5, temperature 60 °C, and ratio (v/w, mL/g) of extraction solvent to raw material 35. The isoelectric point of the pH was determined to be about 4.4 and 10.2, by measuring the protein content of PSAF solutions at different pH values. The amino acid composition of PSAF was determined by high performance liquid chromatography (HPLC), and the results suggested that the species of amino acids contained in the PSAF was complete. Sodium dodecyl sulphate polyacrylamide gel electrophoresis (SDS–PAGE) analysis showed the molecular weight was mainly between 40 and 55 kDa, and Fourier-transform infrared spectroscopy (FTIR) characterized prevalent protein absorption peaks. PSAF exhibited potent scavenging activities against DPPH assays, via targeting of hydroxyl and superoxide radicals, while chelating Fe2+ activity and demonstrating weak reducing power. This work revealed that PSAF possessed potential antioxidant activity in vitro, suggesting potential for use of PSAF as a natural antioxidant.

Effects of Ingredient Incorporation into Sausage Meat on the Micellarisation and uptake of α-tocopherol by Caco-2 Human Intestinal Cells

2008 ◽  
Vol 14 (1) ◽  
pp. 79-86 ◽  
Author(s):  
O. Kenny ◽  
Y. O'Callaghan ◽  
N.M. O'Brien
Keyword(s):  
Olive Oil ◽  
Cellular Uptake ◽  
Sunflower Oil ◽  
Cell Model ◽  
Linseed Oil ◽  
Meat Products ◽  
In Vitro Digestion ◽  
Soya Protein ◽  
Two Phase

Ingredients are incorporated into meat and meat products to produce a ``healthier'' product. However, the effect of ingredient addition on availability of nutrients endogenous to foods is generally not considered. This study investigated the availability and cellular uptake of α-tocopherol from supplemented sausages with the aid of an in vitro digestion procedure coupled with a Caco-2 cell model. Sausages were formulated with the addition of 3% or 10% ingredients (wheat bran, oat bran, soya protein, whey protein, olive oil, linseed oil, sunflower oil, and wheatgerm oil) and subjected to a two-phase in vitro system that simulates the digestive process in humans. Micelles were isolated from the digestate by ultracentrifugation. Of the ingredients selected for addition to sausage meat, only sunflower oil, and wheatgerm oil enhanced the micellarization of α-tocopherol, resulting in increased transfer from the test food to micelles. When ingredients were added at the 3% supplementation level, olive oil enhanced cellular uptake of α-tocopherol. Cellular uptake was not enhanced further with higher oil supplementation (3% vs. 10%). These results indicated that addition of ingredients to sausages (fibres, protein derivatives or vegetable oils) did not have a detrimental effect on α-tocopherol uptake and olive oil at the 3% supplementation level enhanced α-tocopherol availability.

scholarly journals Extraction, Characterization and Antioxidant Activity in vitro of Proteins from Semen Allii Fistulosi

2018 ◽  
Author(s):  
Min Zuo ◽  
Xiaoxiao Liu ◽  
Di Liu ◽  
Hangyun Zhao ◽  
Lulu Xuan ◽  
...  
Keyword(s):  
Antioxidant Activity ◽  
High Performance ◽  
Orthogonal Design ◽  
Polyacrylamide Gel Electrophoresis ◽  
Sodium Dodecyl ◽  
Reducing Power ◽  
Raw Material ◽  
Optimum Conditions ◽  
Extraction Solvent

Semen Allii Fistulosi is the seed of Allium fistulosum L. of the Liliaceae family. The purpose of this study was to extract, characterize, and evaluate the antioxidant activity in vitro of proteins from Semen Allii Fistulosi (PSAF). Using single factor and orthogonal design, the optimum conditions of extraction were determined to be as follows: extraction time 150 min, pH 8.5, temperature 60℃, and ratio (v/w, mL/g) of extraction solvent to raw material 35. The isoelectric point of the pH was determined to be about 4.4 and 10.2, by measuring the protein content of PSAF solutions at different pH. The amino acid composition of PSAF was determined by high performance liquid chromatography (HPLC), and the results suggested that the species of amino acids contained in the PSAF was complete. Sodium dodecyl sulphate polyacrylamide gel electrophoresis (SDS–PAGE) analysis showed the molecular weight was mainly between 40 and 55 kDa, and Fourier-transform infrared spectroscopy (FTIR) characterized prevalent protein absorption peaks. PSAF exhibited potent scavenging activities against DPPH assays, via targeting of hydroxyl and superoxide radicals, while chelating Fe2+ activity, and demonstrating weak reducing power. This work revealed that PSAF possessed potential antioxidant activity in vitro, suggesting potential for use of PSAF as a natural antioxidant.

scholarly journals Effects of Different Herbal Teas on Reducing the Bioaccessibility of Methylglyoxal in Crackers under Stimulated Gastrointestinal Digestive System

2021 ◽  
Vol 11 (2) ◽  
pp. 3421-3429
Keyword(s):  
High Performance Liquid Chromatography ◽  
Liquid Chromatography ◽  
High Performance ◽  
Digestive System ◽  
In Vitro Digestion ◽  
In Vitro System ◽  
Snack Foods

The present study aims to determine the bioaccessibility of methylglyoxal (MGO) in crackers and investigate the effects of herbal teas on reducing the formation of MGO under in vitro digestion system. Different herbal teas were added in crackers to reduce MGO formation under in vitro system. MGO levels were determined by High-Performance Liquid Chromatography. The amounts of MGO in crackers samples at initial and after in vitro digestion were ranged from 51 to 104 µg/100 g and 274 to 408 µg / 100 g, respectively. After in vitro digestion, the bioaccessibility of MGO values was increased up to 628%. Also, it was found that polyphenol-rich herbal teas such as black, green, turmeric, and rosehip significantly reduced MGO bioaccessibility in crackers. The addition of these herbal teas as antioxidants in cracker formulations or consumption of herbal teas along with snack foods may be recommended.

scholarly journals After In Vitro Digestion, Jackfruit Flake Affords Protection against Acrylamide-Induced Oxidative Damage

Molecules ◽  
2019 ◽  
Vol 24 (18) ◽  
pp. 3322 ◽  
Author(s):  
Daofeng Qu ◽  
Chu Liu ◽  
Mengxue Jiang ◽  
Lifang Feng ◽  
Yuewen Chen ◽  
...  
Keyword(s):  
Oxidative Stress ◽  
Oxidative Damage ◽  
High Performance ◽  
In Vitro Digestion ◽  
Protective Effects ◽  
Physical Damage ◽  
Before And After ◽  
High Content Analysis ◽  
High Level

Some studies have demonstrated that acrylamide (AA) was correlated with oxidative stress, resulting in physical damage. The jackfruit flake was an immature pulp that contained a high level of antioxidant activity. This study aimed to assess the defensive efficacy of jackfruit flake in AA-induced oxidative stress before and after simulated gastrointestinal digestion. Our results indicate that the total polyphenol content of Jackfruit flake digest (Digestive products of jackfruit flake after gastrointestinal, JFG) was diminished; however, JFG had raised the relative antioxidant capacity compared to Jackfruit flake extract (JFE). Additionally, the results of High Performance Liquid Chromatography-Mass Spectrometry (HPLC-MS) implied that a proportion of compounds were degraded/converted into other unknown and/or undetected metabolites. Further, by high content analysis (HCA) techniques, JFG markedly reduced cytotoxicity and excessive production of reactive oxygen species (ROS) in cells, thereby alleviating mitochondrial disorders. In this study, it may be converted active compounds after digestion that had preferable protective effects against AA-induced oxidative damage.

Effects of dairy processing on phospholipidome, in-vitro digestion and Caco-2 cellular uptake of bovine milk

2021 ◽  
pp. 130426
Author(s):  
Bolin Mou ◽  
Liu Yuanyuan ◽  
Wenqing Yang ◽  
Shuang Song ◽  
Cai Shen ◽  
...  
Keyword(s):  
Cellular Uptake ◽  
Bovine Milk ◽  
In Vitro Digestion ◽  
Dairy Processing

Impact of food components during in vitro digestion of silver nanoparticles on cellular uptake and cytotoxicity in intestinal cells

2015 ◽  
Vol 396 (11) ◽  
pp. 1255-1264 ◽  
Author(s):  
Dajana Lichtenstein ◽  
Johanna Ebmeyer ◽  
Patrick Knappe ◽  
Sabine Juling ◽  
Linda Böhmert ◽  
...  
Keyword(s):  
Silver Nanoparticles ◽  
Cellular Uptake ◽  
In Vitro Digestion ◽  
Intestinal Cells ◽  
Digestion Process ◽  
X Ray Scattering ◽  
Food Components ◽  
Uptake Studies

Abstract Because of the rising application of nanoparticles in food and food-related products, we investigated the influence of the digestion process on the toxicity and cellular uptake of silver nanoparticles for intestinal cells. The main food components – carbohydrates, proteins and fatty acids – were implemented in an in vitro digestion process to simulate realistic conditions. Digested and undigested silver nanoparticle suspensions were used for uptake studies in the well-established Caco-2 model. Small-angle X-ray scattering was used to estimate particle core size, size distribution and stability in cell culture medium. Particles proved to be stable and showed radii from 3.6 to 16.0 nm. Undigested particles and particles digested in the presence of food components were comparably taken up by Caco-2 cells, whereas the uptake of particles digested without food components was decreased by 60%. Overall, these findings suggest that in vivo ingested poly (acrylic acid)-coated silver nanoparticles may reach the intestine in a nanoscaled form even if enclosed in a food matrix. While appropriate for studies on the uptake into intestinal cells, the Caco-2 model might be less suited for translocation studies. Moreover, we show that nanoparticle digestion protocols lacking food components may lead to misinterpretation of uptake studies and inconclusive results.

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