scholarly journals Quality of Soybean Products in Terms of Essential Amino Acids Composition

Molecules ◽  
2021 ◽  
Vol 26 (16) ◽  
pp. 5071
Author(s):  
Wanda Kudełka ◽  
Małgorzata Kowalska ◽  
Marzena Popis

The content of protein, moisture content and essential amino acids in conventional and genetically modified soybean grain and selected soybean products (soybean pâté, soybean drink, soybean dessert, tofu) was analyzed in this paper. The following comparative analysis of these products has not yet been carried out. No differences were observed in the amino acid profiles of soybeans and soybean products. The presence of essential amino acids was confirmed except for tryptophan. Its absence, however, may be due not to its absence in the raw material, but to its decomposition as a result of the acid hydrolysis of the sample occurring during its preparation for amino acid determination. Regardless of the type of soybean grain, the content of protein, moisture content and essential amino acids was similar (statistically insignificant difference). Thus, the type of raw material did not determine these parameters. There was a significant imbalance in the quantitative composition of essential amino acids in individual soybean products. Only statistically significant variation was found in genetically modified and conventional soybean pâté. Moreover, in each soy product their amount was lower irrespective of the raw material from which they were manufactured. Therefore, the authors indicate the necessity of enriching soybean products with complete protein to increase their nutritional value.

2015 ◽  
Vol 58 (1) ◽  
pp. 30-39
Author(s):  
Emmanuel Ilesanmi Adeyeye

The amino acids composition of the brain and eyes of the mature Turkey-hen (Meleagris gallopavo L.),  were determined on dry weight basis. Total essential amino acids ranged from 35.1-36.0 g/100 g as 49.5-49.8% of the total amino acids. The amino acid score showed that lysine ranged from 0.76-0.91 (on whole hen’s egg comparison), 0.85-1.03 (on provisional essential amino acid scoring pattern), and 0.81-0.98 (on suggested requirement of the essential amino acid of a preschool child). The predicted protein efficiency ratio was 1.94-2.41, whilst essential amino acid index range was 1.06-1.08 and the calculated isoelectric point range was 3.97-4.18. The correlation coefficient (rxy) was positively high and significant at r = 0.01 for the total amino acids, amino acid scores (on the whole hen’s egg comparisons made) and the isoelectric point. On the whole, the eyes were better in 12/18 or 66.7% parameters of the amino acids than the brain of Turkey-Hen.


2019 ◽  
pp. 82-87
Author(s):  
M. I. Shanayda

Researching of the amino acids contents in medicinal plant raw materials is an important task of pharmaceutical science and practice, because complexes of amino acids used for the corrections of hepatobiliary disorders, nervous and cardiovascular systems. The aim of this study was to conduct a qualitative and quantitative analysis of amіno acids composition of these representatives. The article presents the results of the amino acid composition investigation of the above-ground parts of 10 species belonging to Lamiaceae Juss. Family (Ocimum, Hyssopus, Dracocephalum, Lophanthus, Monarda and Satureja genera). By paper chromatography it was identified several amino acids. HPLC analyzes revealed the content and composition of more than 20 amino acids. The highest total amino acid composition was found in the herb of the genus Ocimum. It was identified 10 essential and non-essential 9 amino acids in each herb, and 3 nonproteinogenic amino acids also. It was established that in all plant materials dominate quantitatively nonessential amino acids (asparagine, proline, glutamic and aspartic acid). Among the essential amino acids arginine and threonine predominate.


Food Research ◽  
2019 ◽  
pp. 617-621 ◽  
Author(s):  
A. Doss ◽  
P.S. Tresina ◽  
V.R. Mohan

Tubers of wild yam (Dioscorea alata, D. bulbifera var. vera, D. esculenta, D. oppositifolia var. oppositifolia, D. oppositifolia var. dukhumensis, D. pentaphylla var. pentaphylla, D. spicata, D. tomentosa and D. wallichi) were analyzed for its amino acids composition. Asparatic acid and glutamic acid were found to be predominant among all the investigated Dioscorea species which ranged from 5.21 to 9.36 and 3.20 to 8.12 g/100 g protein respectively. The essential amino acids such as isoleucine, phenylalanine tyrosine, histidine, isoleucine and valine were found to be more or less higher than FAO/WHO (1991) requirement pattern. Thus, the present investigation demonstrated that the Dioscorea spp. can act as a good source of amino acid


2017 ◽  
Vol 61 ◽  
pp. 23-35 ◽  
Author(s):  
Emmanuel Ilesanmi Adeyeye

This paper reports on amino acid profiles of the flesh of heterosexuals of porcellanids collected from the Atlantic Ocean at Orimedu beach in Ibeju-Lekki, Lagos, Nigeria. Results showed that high values of amino acids were observed in the heterosexual flesh of Neopetrolisthes maculatus (g 100g-1 protein): 17.7 – 17.8 (Glu), 9.90 – 10.0 (Asp), 8.70 – 9.07 (Arg), 7.23 – 7.94 (Leu) and 5.81 – 6.06 (Gly). Total essential amino acid values ranged from 45.2 – 46.2 g 100-1g. Predicted protein efficiency ratio was 3.82 – 4.14, the range of essential amino acid index was 86.9 – 89.9, the biological values ranged from 83.0 – 86.3. The Lys/Trp was 3.31-4.27. Serine was limiting amino acid (0.513 – 0.516) in the egg score comparison; under the essential amino acids scores, Lys (0.840) was limiting in female but Val (0.823) was limiting in the male; Lys (0.796 – 0.905) was limiting in both samples in the pre-school child amino acid requirements. It was observed that out of the twenty parameters determined, male flesh was more concentrated in 60% values than the female flesh and 40% better in female than male. Correlation coefficient result showed that significant differences existed in the amino acids composition at r = 0.01 of the N. maculatus samples.


2020 ◽  
Vol 79 ◽  
pp. 23-39
Author(s):  
Emmanuel Ilesanmi Adeyeye ◽  
Olajide Ayodele ◽  
Joshua Iseoluwa Orege

Amino acids composition of Thryonomysswingerianus is reported. Whereas protein values (g100g-1) were liver (74.1), kidney (91.5), heart (84.6); corresponding total amino acid values were 93.5, 83.2 and 80.6. True protein from the crude protein of the samples ran thus: liver>kidney>heart. Of the twenty parameters reported on, liver was best in 12/20 (60.0%), kidney and heart both shared the second position of 4/20(20%) each. Among the essential amino acids, leucine predominated in both liver (7.96g100g-1 protein) and kidney (8.11g100g-1 protein) but valine (6.21g100g-1 protein) predominated in the heart. The P-PER values were; P-PER1: 2.78 (liver), 2.91(kidney), 0.716 (heart) and P-PER2: 2.71 (liver), 2.90 (kidney), 0.564 (heart). However, there was a reverse between liver and kidney in the EAAI values with liver (92.0) > kidney (90.2) > heart (87.6) with corresponding BV values of 88.5 > 86.6 > 83.7. In the amino acids scoring pattern, Ser was limiting in liver (0.533) and heart (0.394) but Thr (0.490) in kidney in whole hen’s egg score comparison; in FAO/WHO scoring standards, Thr was limiting in liver (0.988) and kidney (0.625) but Leu (0.459) in heart. In pre-school requirements, liver recorded no limiting amino acid whereas Thr was limiting in kidney (0.735) and Leu was limiting in the heart (0.486). T.swingerianus red viscera was compared with the red viscera of livestock animals (cattle, sheep and pork) as well as FAO/WHO/UNU standards for total essential amino acids. Our results when compared with the livestock red viscera (without Trp) and FAO/WHO/UNU (g100g-1 protein), we have heart: grasscutter/cattle/sheep/pig:45.3 /46.0/42.7/46.6; kidney: grasscutter/cattle/sheep/pig: 47.6/43.8/42.5/46.7; liver: grasscutter/cattle/sheep/pig: 50.7/47.7/41.5/47.5 and grasscutter liver/kidney/heart/ FAO/WHO/UNU:50.7/47.6/45.3/32.8 showing that all the red viscera values in T.swingerianus were better than the essential amino acids in the FAO/WHO/UNU standards and livestock red viscera. Statistical values showed that significant differences existed among the samples at r=0.01.


Author(s):  
L. V. Vronska ◽  
A. Ye. Demyd

The aim of the research was to study the amino acid profile of the phaseolus vulgaris pods and extract prepared of them. Materials and methods. Five samples of raw material of phaseolus vulgaris pods (erect herbaceous bushes varieties with white seeds) were collected in the Ternopil and Volyn regions, dry extract of phaseolus vulgaris pods was obtained according to previously developed technology. The study of amino acid composition of the raw materials of phaseolus vulgaris pods and extract prepared of them was carried out using a thin-layer chromatography (TLC) and a high-performance liquid chromatography (HPLC). Results and discussion. The better separation of amino acids in TLC-research of the raw material of phaseolus vulgaris pods was observed in the system of solvents isopropanol - formic acid - water (40: 2: 10). As a result of the study, aspartic and glutamic acids, glycine, valine, tyrosine and leucine were identified. The amino acid profile of the studied samples of raw material is quite homogeneous in composition: 7 essential amino acids (histidine, threonine, valine, methionine, phenylalanine, isoleucine and leucine) and 8 non-essential amino acids (aspartic and glutamic acids, arginine, serine, glycine, alanine, tyrosine and proline); lysine was found among the bound amino acids in the 4th sample of raw material. Proline predominates in all samples of raw materials among free amino acids. Among the bound amino acids the content of glutamic acid, which is the product of the glutamine hydrolysis, is the highest. The content of glycine, serine and alanine is also high. Among the essential amino acids, leucine, phenylalanine, histidine, threonine, isoleucine, valine were determined in content descending order. The quantitative determination of amino acids in the extract of phaseolus vulgaris pods proved that the content of proline was the highest (12.47 mg / g); the content of some compounds was also high: aspartic (5.41 mg / g) and glutamic (3.41 mg / g) acids, arginine (5.10 mg / g; both in free and bound forms), glycine (1.02 mg / g) and serine (1.04 mg / g). Among the essential amino acids in the extract, the amount of valine (0.80 mg / g), phenylalanine (0.67 mg / g), threonine (0.66 mg / g), leucine (0.63 mg / g) and isoleucine (0.58 mg / g) was a little different. The total content of amino acids in the extract was 3.2%. Conclusions. 1. The amino acid profile of five samples of phaseolus vulgaris pods was studied by the HPLC method. It has been established that the composition is quite homogeneous, and the total content varies within 0.7-1.1%. 2. In the dry extract of phaseolus vulgaris pods the content of 5 essential and 7 non-essential amino acids has been determined. The content of free amino acids in the extract is 0.52%; the total content of free and bound amino acids is 3.2%. 3. When studying the stability and establishing the shelf life of the dry extract of phaseolus vulgaris pods, it is necessary to take into account the presence of free amino acids and protein substances.  


2020 ◽  
Vol 5 (3) ◽  
pp. 185-191
Author(s):  
H. K. Pandey ◽  
Anchala Guglani ◽  
G. C. Kharakwal ◽  
G. Balakrishna ◽  
Madhu Bala

Less explored Allium species are being used as green vegetables and as a condiment by the local inhabitant of high-altitude areas of Uttarakhand. In the present study, four economically important less explored wild Allium species viz. A. auriculatum, A. ampeloprasum, A. ascalonicum and A. rubellum have screened for their amino acid contents by High-performance liquid chromatography. The hydrochloric acid hydrolysate of these four species, the Allium amino acids were derivatised with phenylisothiocyanate resulting phenylthiocarbamyl derivatives and separated on a reverse-phase column by gradient elution with aqueous buffer and acetonitrile-water (60:40 v/v) and detected in UV region at 254 nm. The Pico-tag (3.9 × 300 mm) C18 column equilibrated with the solvents. The elution of all amino acid derivatives was achieved in 12 min using gradient elution by increasing concentration of aqueous buffer and acetonitrile-water. Total seventeen amino acids were present in these Allium species. The ratio of essential amino acids to total amino acids found 1:2.14 in Allium auriculatum, 1:2.35 in Allium ampeloprasum, 1:1.38 in A. ascalonicum and 1:3.44 in Allium rubellum. These less explored Allium species contained substantial amount of essential and non-essential amino acids. Among these Allium species, Allium auriculatum and Allium rubellum found most promising as far as essential and non-essential amino acids composition concerned.


Author(s):  
E. C. Kalu ◽  
O. C. Ikewuchi ◽  
C. J. Ayalogu

The minerals, vitamins and Amino acids composition of Melanthera biflora leaves were investigated using standard methods. The result shows that out of the eleven vitamins detected; only vitamin C was found in moderately high amount (99.37 mg/100 g), while the rest were in insignificant amount. Ten minerals element were detected, including iron (14.63) copper (2.32). Cobalt (0.04) manganese (5.13), calcium (93.52) magnesium (126.17), potassium (71.39) sodium (32.37), phosphorus 36.50, selenium (0.006) all in mg/100 g. The leaves have essentials and non-essential amino acid of 47.5% and 45.94% respectively. It has all the essential amino acids except tryptophan. This result shows that the leaves contain an appreciable amount of amino acids, vitamins and minerals that could be included in diets to supplement our daily dietary allowance.


Author(s):  
P.B. Zamudio-Flores ◽  
M. Hernández -Gonzaléz ◽  
V.G. García- Cano

Insects can be used as an easily accessible food supplement; however, for nutritional purposes it is necessary to consider its protein content, amino acids composition and vitamin content. In this study a nutritional characterization (protein content, amino acid profile and hydrosoluble and liposoluble vitamins) was performed to the Brachystola magna (Girard) grasshopper in four stages of its biological cycle (egg, nymph 3, nymph 4 and adult). In the adult stage the highest values of protein content (> 59%) were observed compared with the other stages of development. At all stages of B. magna the presence of 9 out of the 10 essential amino acids were detected (just tryptophan amino acid was absent). Significant increases in the fat-soluble vitamins (A, D and E) were observed as the stage of development of B. magna increased, with the increase of vitamin E of ≈ 662% in the adult stage; however, the water-soluble vitamins of the B complex remained constant at all stages, but vitamin C, which increased significantly in the adult stage of this insect cycle. Results suggest that Brachystola magna (Girard) could be a potential source of consumption for people with specific health needs.


2021 ◽  
Vol 285 ◽  
pp. 02046
Author(s):  
Yulia Goncharova ◽  
Svetlana Verechagina ◽  
Nikolay Ochkas ◽  
Sergey Goncharov

Rice contains antioxidants, minerals, carbohydrates, lipids, vitamins that make it a functional food. A dozen times higher content of antioxidants was noted in black-grain and red-grain rice varieties as compared to white-grain ones. In terms of protein content, color and white-grain varieties do not differ significantly. The protein composition largely determines the nutritional value and functional properties, as well as the texture and taste of rice. Although rice contains less protein than many cereals, its biological value is highest due to balanced composition of amino acids. Nutritional value of different rice varieties varies greatly due to different amino acid composition. Determination of amino acid composition was carried out on devices Kapel 105 - M. In the work used 50 collection samples of rice. The variability was studied and the sources were identified among domestic rice varieties. To create a new generation varieties with more balanced amino acid composition, it is necessary both to isolate sources with a high content of individual amino acids, and varieties with high characteristics in terms of content complex of amino acids, especially essential ones.


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