scholarly journals Gadolinium-Based Paramagnetic Relaxation Enhancement Agent Enhances Sensitivity for NUS Multidimensional NMR-Based Metabolomics

Molecules ◽  
2021 ◽  
Vol 26 (17) ◽  
pp. 5115
Author(s):  
Chandrashekhar Honrao ◽  
Nathalie Teissier ◽  
Bo Zhang ◽  
Robert Powers ◽  
Elizabeth M. O’Day
Keyword(s):  
Limit Of Detection ◽  
Signal To Noise Ratio ◽  
Paramagnetic Relaxation ◽  
Paramagnetic Relaxation Enhancement ◽  
Intensity Measurements ◽  
Metabolite Concentrations ◽  
Broad Array ◽  
The Mean ◽  
Signal Intensity Measurement ◽  
Micromolar Range

Gadolinium is a paramagnetic relaxation enhancement (PRE) agent that accelerates the relaxation of metabolite nuclei. In this study, we noted the ability of gadolinium to improve the sensitivity of two-dimensional, non-uniform sampled NMR spectral data collected from metabolomics samples. In time-equivalent experiments, the addition of gadolinium increased the mean signal intensity measurement and the signal-to-noise ratio for metabolite resonances in both standard and plasma samples. Gadolinium led to highly linear intensity measurements that correlated with metabolite concentrations. In the presence of gadolinium, we were able to detect a broad array of metabolites with a lower limit of detection and quantification in the low micromolar range. We also observed an increase in the repeatability of intensity measurements upon the addition of gadolinium. The results of this study suggest that the addition of a gadolinium-based PRE agent to metabolite samples can improve NMR-based metabolomics.

SERUM TRIIODOTHYRONINE DETERMINATION BY SATURATION ANALYSIS

1973 ◽  
Vol 72 (4) ◽  
pp. 714-726 ◽  
Author(s):  
A. Burger ◽  
B. Miller ◽  
C. Sakoloff ◽  
M. B. Vallotton
Keyword(s):  
Ionic Strength ◽  
Limit Of Detection ◽  
Improved Method ◽  
Accuracy Of Measurement ◽  
Tracer Amount ◽  
The Mean ◽  
Physiological Values ◽  
Hyperthyroid Patients ◽  
Solvent Blank

ABSTRACT An improved method for the determination of serum triiodothyronine (T3) has been developed. After addition of a tracer amount of the hormone, T3 was extracted from 1 ml serum under conditions of pH and ionic strength which favoured T3 extraction (89%) over thyroxine (T4) extraction (58%). Chromatography of the extracted material on Sephadex LH-20 separated T3 completely from residual T4. The T3 eluate was dried, then re-dissolved in 0.5 ml NaOH 0.04 n. To 0.2 ml duplicate aliquots, a standard amount of TBG was added for the competitive protein analysis. After one hour incubation at 4°C, separation of bound from free T3 was achieved on small Sephadex G-25 columns. Overall recovery was 67 ± 10.8% and correction for the loss was made. The solvent blank was 37 ± 27 (sd) ng/100 ml. Accuracy of measurement of known quantities of T3 added to serum was 98.4%. The coefficient of variation within the assay was 6.2% and between the assays it was 11.4%. The limit of detection (0.1 ng) corresponded to a concentration of 25 ng/100 ml. T4 added to serum did not interfere with T3 determination until high non-physiological values were reached. The mean ± sd serum T3 in 54 euthyroid subjects was 153 ± 58 ng/100 ml and in 24 hyperthyroid patients it was 428 ±186 ng/100 ml; 4 out of the 24 hyperthyroid values were within 2 sd of the mean euthyroid group. All the values found in the euthyroid group were well above the limit of detection of the method.

scholarly journals Structure-Free Validation of Residual Dipolar Coupling and Paramagnetic Relaxation Enhancement Measurements of Disordered Proteins

Biochemistry ◽  
2015 ◽  
Vol 54 (46) ◽  
pp. 6876-6886 ◽  
Author(s):  
Francisco N. Newby ◽  
Alfonso De Simone ◽  
Maho Yagi-Utsumi ◽  
Xavier Salvatella ◽  
Christopher M. Dobson ◽  
...  
Keyword(s):  
Dipolar Coupling ◽  
Residual Dipolar Coupling ◽  
Paramagnetic Relaxation ◽  
Paramagnetic Relaxation Enhancement ◽  
Disordered Proteins

scholarly journals Optimization of SPECT/CT imaging protocols for quantitative and qualitative 99mTc SPECT

2021 ◽  
Vol 8 (1) ◽  
Author(s):  
Dennis Kupitz ◽  
Heiko Wissel ◽  
Jan Wuestemann ◽  
Stephanie Bluemel ◽  
Maciej Pech ◽  
...  
Keyword(s):  
Signal To Noise Ratio ◽  
Quantitative Imaging ◽  
Scatter Correction ◽  
Reconstruction Algorithm ◽  
Weighting Factor ◽  
Quantitative Spect ◽  
Pet Ct ◽  
Spect Acquisition ◽  
The Mean ◽  
A Minor

Abstract Background The introduction of hybrid SPECT/CT devices enables quantitative imaging in SPECT, providing a methodological setup for quantitation using SPECT tracers comparable to PET/CT. We evaluated a specific quantitative reconstruction algorithm for SPECT data using a 99mTc-filled NEMA phantom. Quantitative and qualitative image parameters were evaluated for different parametrizations of the acquisition and reconstruction protocol to identify an optimized quantitative protocol. Results The reconstructed activity concentration (ACrec) and the signal-to-noise ratio (SNR) of all examined protocols (n = 16) were significantly affected by the parametrization of the weighting factor k used in scatter correction, the total number of iterations and the sphere volume (all, p < 0.0001). The two examined SPECT acquisition protocols (with 60 or 120 projections) had a minor impact on the ACrec and no significant impact on the SNR. In comparison to the known AC, the use of default scatter correction (k = 0.47) or object-specific scatter correction (k = 0.18) resulted in an underestimation of ACrec in the largest sphere volume (26.5 ml) by − 13.9 kBq/ml (− 16.3%) and − 7.1 kBq/ml (− 8.4%), respectively. An increase in total iterations leads to an increase in estimated AC and a decrease in SNR. The mean difference between ACrec and known AC decreased with an increasing number of total iterations (e.g., for 20 iterations (2 iterations/10 subsets) = − 14.6 kBq/ml (− 17.1%), 240 iterations (24i/10s) = − 8.0 kBq/ml (− 9.4%), p < 0.0001). In parallel, the mean SNR decreased significantly from 2i/10s to 24i/10s by 76% (p < 0.0001). Conclusion Quantitative SPECT imaging is feasible with the used reconstruction algorithm and hybrid SPECT/CT, and its consistent implementation in diagnostics may provide perspectives for quantification in routine clinical practice (e.g., assessment of bone metabolism). When combining quantitative analysis and diagnostic imaging, we recommend using two different reconstruction protocols with task-specific optimized setups (quantitative vs. qualitative reconstruction). Furthermore, individual scatter correction significantly improves both quantitative and qualitative results.

Development of a solid-phase extraction – spectrofluorimetric method for trace glutathione determination

2011 ◽  
Vol 89 (4) ◽  
pp. 517-523 ◽  
Author(s):  
Ke-Jing Huang ◽  
Cong-Hui Han ◽  
Ying-Ying Wu ◽  
Chao-Qun Han ◽  
De-Jun Niu ◽  
...  
Keyword(s):  
Solid Phase Extraction ◽  
Limit Of Detection ◽  
Solid Phase ◽  
Signal To Noise Ratio ◽  
Calibration Graph ◽  
Extraction Column ◽  
Phase Extraction ◽  
Selective Reaction ◽  
Spectrofluorimetric Method ◽  
Relative Standard

A simple and efficient solid-phase extraction – spectrofluorimetric method has been developed to determine glutathione (GSH). Fluorescent probe N-(4,4-difluoro-5,7-dimethyl-4-bora-3a,4a-diaza-s-indacene-3-yl)methyl)iodoacetamide (BODIPY Fl-C1-IA) was used as the derivatization reagent. The procedure was based on a BODIPY Fl-C1-IA selective reaction with GSH to form the highly fluorescent product BODIPY Fl-C1-IA–GSH, using a solid-phase extraction column and spectrofluorimetric determination. The variables affecting analytical performance were studied and optimized. The calibration graph using the preconcentration system for GSH was linear over the range of 1–200 nmol/L with a limit of detection of 0.05 nmol/L (signal-to-noise ratio = 3). The relative standard deviation for six replicate determinations of GSH at the 100 nmol/L concentration level was 3.9%. The method was applied to water samples and average recoveries between 87.5% and 111.5% were obtained for spiked samples.

scholarly journals 2D In-Plane Sensitive Hall-Effect Sensor

Proceedings ◽  
2018 ◽  
Vol 2 (13) ◽  
pp. 711
Author(s):  
Siya Lozanova ◽  
Ivan Kolev ◽  
Avgust Ivanov ◽  
Chavdar Roumenin
Keyword(s):  
Hall Effect ◽  
Signal To Noise Ratio ◽  
Hall Effect Sensor ◽  
Orthogonal Components ◽  
The Mean ◽  
The Cross ◽  
The Magnetic Field ◽  
Outer Contact ◽  
Hall Elements ◽  
Ic Technology

A new 2D (two-dimensional) in-plane sensitive Hall-effect sensor comprising two identical n-Si Greek-crosses is presented. Each of the crosses contains one central square contact and, symmetrically to each of their four sides, an outer contact is available. Outer electrode from one configuration is connected with the respective opposite contact from the other configuration, thus forming four parallel three-contact (3C) Hall elements. These original connections provide pairs of opposite supply currents in each of the cross-Hall structure. Also the obligatory load resistors in the outer contacts of 3С Hall elements are replaced by internal resistances of crosses themselves. The samples have been implemented by IC technology, using four masks. The magnetic field is parallel to the structures’ plane. The couples of opposite contacts of each Greek-cross are the outputs for the two orthogonal components of the magnetic vector at sensitivities S ≈ 115 V/AT whereas the cross-talk is very promising, reaching no more than 2.4%. The mean lowest detected magnetic induction B at a supply current Is = 3 mA over the frequency range f ≤ 500 Hz at a signal to noise ratio equal to unity, is Bmin ≈ 14 μT.

scholarly journals Adaptive Noise Cancellation of an Analog Fiber Optic Link

2021 ◽  
Author(s):  
Mohammad Nazrul Islam
Keyword(s):  
Optical Fiber ◽  
Fiber Optic ◽  
Signal To Noise Ratio ◽  
Optical Power ◽  
Noise Cancellation ◽  
Adaptive Noise Cancellation ◽  
Fiber Link ◽  
The Mean ◽  
Simulation Results ◽  
Adaptive Noise

There are three dominant noise mechanisms in an analog optical fiber link. These are shot noise that is proportional to the mean optical power, relative intensity noise (RIN) that is proportional to the square of the instanteaneous optical power. This report describes an adaptive noise cancellation of these dominant noise processes that persist an analog optical fiber link. The performance of an analog optical fiber link is analyzed by taking the effects of these noise processes. Analytical and simulation results show that some improvement in signal to noise ratio (SNR) and this filter is effective to remove noise adaptively from the optical fiber link.

scholarly journals Development of the Persian version of high-frequency emphasis quick speech in noise

2019 ◽  
Author(s):  
Jamileh Fatahi ◽  
Maryam Amiri Jahromi ◽  
Fahimeh Hajiabolhassan ◽  
Amirsalar Jafarpisheh ◽  
Nariman Rahbar ◽  
...  
Keyword(s):  
High Frequency ◽  
Signal To Noise Ratio ◽  
Mean Value ◽  
Small Sample ◽  
Test Reliability ◽  
Face Validity ◽  
Mean Values ◽  
Speech In Noise ◽  
Persian Version ◽  
The Mean

Background and Aim: The quick speech in noise (Q-SIN) test shows the difficulty of spee­ch perception in noise by specifying signal to noise ratio (SNR) loss. Although the Persian version of Q-SIN has been already constructed, the high-frequency emphasis version of this test is not available. The present study aimed to construct six lists with high-frequency emphasis and implement it. Methods: We are going to prepare a high-frequ­ency emphasis version of Q-SIN and then test it on a small sample. First, researchers designed the relevant sentences; then experts examined their content and face validity. According to the criteria for developing the Q-SIN test, six lists with high-frequency emphasis were prepared. The test was examined on 26 (13 male and 13 female), 18−35 years old individuals with nor­mal hearing. To determine the test reliability, it was re-administered three weeks later with the same conditions. Results: Of 76 sentences prepared, 36 sentences received enough credit after determination of their content and face validity. These 36 senten­ces were used to make 6 lists. The mean value of SNR50 in the Persian language was obtained -4 dB. The mean values of SNR loss in 6 lists were -1.65, -1.8, -2.23, -1.61, -2.38 and -2.07. The results showed equivalency of lists 1, 2, 3, 4, and 6. Examination of test-retest reliability indicated that all lists except the list 2were reliable. Conclusion: The lists of 1, 3, 4, and 6 are reli­able and equivalent and can be used in clinical application.

scholarly journals Quantitative Ausmessung des Brom-Affinitätskontinuums und Bestimmung der Detachment-und Attachment-Querschnitte

1970 ◽  
Vol 25 (11) ◽  
pp. 1617-1626 ◽  
Author(s):  
H. Frank ◽  
M. Neiger ◽  
H.-P. Popp
Keyword(s):  
Cross Section ◽  
Cross Sections ◽  
Electron Affinity ◽  
Elastic Cross Section ◽  
Electron Atom ◽  
Intensity Measurements ◽  
Long Wave ◽  
The Mean ◽  
Intense Electron

Abstract A wall stabilized low-current cylindric arc was used to produce the radiation of the negative Bromine-ions. The radiation consists of an affinity-continuum with a long-wave threshold of 3682 Å, yielding an electron affinity for Bromine of 3.366 eV, and of an intense electron-atom Bremsstrahlung in the visible. Intensity measurements of the continua allow the determination of the photo-detachment-and attachment-cross-sections of Bromine and also the determination of the mean elastic cross-section of electrons against Bromine atoms.

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