scholarly journals Pouteria lucuma Pulp and Skin: In Depth Chemical Profile and Evaluation of Antioxidant Activity

Molecules ◽  
2021 ◽  
Vol 26 (17) ◽  
pp. 5236
Author(s):  
Milena Masullo ◽  
Antonietta Cerulli ◽  
Cosimo Pizza ◽  
Sonia Piacente
Keyword(s):  
Antioxidant Activity ◽  
Agricultural Waste ◽  
Metabolite Profile ◽  
Careful Analysis ◽  
2D Nmr ◽  
Negative Ion ◽  
Specialized Metabolites ◽  
Esi Ms ◽  
Ms Analysis ◽  
Negative Ion Mode

Pouteria lucuma Ruiz and Pav., known as the ‘Gold of the Incas’ or ‘lucuma’, is a subtropical fruit belonging to the Sapotaceae family, with a very sweet flavor, used to prepare cakes, ice creams as well as in the baking and dairy industries. To date, the content of primary metabolites is known, but little information is reported about the composition in specialized metabolites. Moreover, no study is reported on skin which represent an important agricultural waste due to the high demand for lucuma. In order to have a preliminary metabolite profile of Pouteria lucuma, the extracts of pulp and skin have been analyzed by LC-ESI/LTQOrbitrap/MS/MS in negative ion mode. The careful analysis of the accurate masses, of the molecular formulas and of the ESI/MS spectra allowed to identify specialized metabolites belonging to phenolic, flavonoid and polar lipid classes. The LC-MS/MS analysis guided the isolation of compounds occurring in the pulp extract whose structures have been characterized by spectroscopic methods including 1D- and 2D-NMR experiments and ESI-MS analysis. Furthermore, the phenolic content of the extracts along with the antioxidant activity of extracts and isolated compounds was evaluated.

Dependence of matrix effect on ionization polarity during LC–ESI–MS analysis of derivatized amino acids in some natural samples

2017 ◽  
Vol 23 (5) ◽  
pp. 245-253 ◽  
Author(s):  
Maarja-Liisa Oldekop ◽  
Riin Rebane ◽  
Koit Herodes
Keyword(s):  
Amino Acids ◽  
Matrix Effect ◽  
Negative Ion ◽  
Herbal Extracts ◽  
Esi Ms ◽  
Ms Analysis ◽  
The Matrix ◽  
Negative Ion Mode ◽  
Positive Ion ◽  
Sample Dilution

Matrix effect, the influence of co-eluting components on the ionization efficiency of the analyte, affects the trueness and precision of the LC–ESI–MS analysis. Derivatization can reduce or eliminate matrix effect, for example, diethyl ethoxymethylenemalonate (DEEMM) derivatives have shown less matrix effect compared to other derivatives. Moreover, the use of negative ion mode can further reduce matrix effect. In order to investigate the combination of derivatization and different ionization modes, an LC–ESI–MS/MS method using alternating positive/negative ion mode was developed and validated. The analyses in positive and negative ion modes had comparable limit of quantitation values. The influence of ESI polarity on matrix effect was investigated during the analysis of 22 DEEMM-derivatized amino acids in herbal extracts and honeys. Sample dilution approach was used for the evaluation of the presence of matrix effect. Altogether, 4 honeys and 11 herbal extracts were analyzed, and the concentrations of 22 amino acids in the samples are presented. In the positive ion mode, matrix effect was observed for several amino acid derivatives and the matrix effect was stronger in honey samples compared to the herbal extracts. The negative ion mode was free from matrix effect, with only few exceptions in honeys (average relative standard deviation over all analytes and matrices was 8%; SD = 7%). The matrix effect was eliminated in the positive ion mode by sample dilution and agreement between concentrations from the two ion modes was achieved for most amino acids. In conclusion, it was shown that the combination of derivatization and negative ion mode can be a powerful tool for minimizing matrix effect in more complicated applications.

scholarly journals Triterpenes from Maytenus macrocarpa and Evaluation of Their Anti-HIV Activity

2006 ◽  
Vol 1 (12) ◽  
pp. 1934578X0600101 ◽  
Author(s):  
Sonia Piacente ◽  
Lourdes Campaner Dos Santos ◽  
Naheed Mahmood ◽  
Cosimo Pizza
Keyword(s):  
Active Compound ◽  
2D Nmr ◽  
Selectivity Index ◽  
Spectroscopic Methods ◽  
Pentacyclic Triterpenes ◽  
Esi Ms ◽  
Ms Analysis ◽  
Anti Hiv ◽  
Hiv 1

A set of pentacyclic triterpenes has been isolated from the bark of Maytenus macrocarpa (Celastraceae). It includes two new olean-12-ene derivatives, namely 3β, 22α-dihydroxy-olean-12-en-30-oic acid (1) and 22α-hydroxy-olean-12-en-3-oxo-30-oic acid (2), and the new urs-12-ene derivative 3-(E)-coumaroyluvaol (7), along with 10 known compounds possessing olean-12-ene, urs-12-ene, lupane and friedelane skeletons. The structures have been elucidated by extensive spectroscopic methods including 1D- and 2D-NMR experiments, as well as ESI-MS analysis. All isolated compounds were tested for anti-HIV activity in C8166 cells infected with HIV-1MN. The most active compound was 22α-hydroxy-12-en-3-oxo-29-oic acid (triterpenonic acid A, 4), with an EC50 value of 1 μg/mL and a selectivity index of 35.

scholarly journals Profiling Murchison Soluble Organic Matter for New Organic Compounds with APPI- and ESI-FT-ICR MS

Life ◽  
2019 ◽  
Vol 9 (2) ◽  
pp. 48 ◽  
Author(s):  
Jasmine Hertzog ◽  
Hiroshi Naraoka ◽  
Philippe Schmitt-Kopplin
Keyword(s):  
Organic Matter ◽  
Negative Ion ◽  
Ion Cyclotron Resonance ◽  
Ms Analysis ◽  
Major Interest ◽  
Polycyclic Aromatic ◽  
Soluble Organic Fraction ◽  
Negative Ion Mode ◽  
Ft Icr Ms ◽  
Ft Icr

The investigation of the abundant organic matter in primitive meteorite such as carbonaceous chondrites is of major interest in the field of origin of life. In this study, the soluble organic fraction of the Murchison meteorite was analyzed by atmospheric pressure photoionization (APPI) and electrospray ionization (ESI) Fourier transform ion cyclotron resonance mass spectrometry (FT-ICR MS), in both detection modes. Such an approach ensured that we obtained an extensive description of the organic matter of the CM2 meteorite. Indeed, while in total close to 16,000 unique features were assigned, only 4% are common to all analyses, illustrating the complementarity of both the detection modes and the ionization sources. ESI FT-ICR MS analysis, in negative-ion mode, ensured to observe specifically CHOS and CHNOS species, whereas the positive-ion mode is more dedicated to the detection of CHNO and CHN species. Moreover, new organomagnesium components were observed in (+) ESI. Eventually, (+) APPI FT-ICR MS analysis was a preferred method for the detection of less polar or nonpolar species such as polycyclic aromatic hydrocarbons but also heteroatom aromatic species composing the organic matter of Murchison.

Positive and negative ion mode ESI-MS and MS/MS for studying drug–DNA complexes

2006 ◽  
Vol 253 (3) ◽  
pp. 156-171 ◽  
Author(s):  
Frédéric Rosu ◽  
Sophie Pirotte ◽  
Edwin De Pauw ◽  
Valérie Gabelica
Keyword(s):  
Negative Ion ◽  
Dna Complexes ◽  
Esi Ms ◽  
Negative Ion Mode

scholarly journals Negative Ion Mode nanoLC-ESI-MS/MS Analyses of Permethylated Sulfated Glycans

BIO-PROTOCOL ◽  
2020 ◽  
Vol 10 (10) ◽  
Author(s):  
Shin-Yi Yu ◽  
Chu-Wen Cheng ◽  
Kay-Hooi Khoo
Keyword(s):  
Negative Ion ◽  
Esi Ms ◽  
Negative Ion Mode

scholarly journals Studies on structural elucidation of delphinium alkaloids by using LC-ESI-MS technique.

2015 ◽  
Vol 11 (3) ◽  
pp. 3399-3411
Author(s):  
Bilal Ahmad Dar ◽  
Mushtaq Ahmad Qurishi ◽  
Aijaz Hussain Kanth
Keyword(s):  
Mass Spectrometry ◽  
Liquid Chromatography ◽  
Negative Ion ◽  
Tentative Identification ◽  
Esi Ms ◽  
Short Run ◽  
Positive Mode ◽  
Fragmentation Patterns ◽  
Negative Ion Mode ◽  
Mode Response

A rapid,sensitive and specific liquid chromatography-electron spray-mass spectrometry (LC-ESI-MS) method to identify the different diester-diterpenoid and monoester type alkaloids from delphinium cashmerianum Collected from Sopi Kargil. Chromatographic separation were achieved on c-18 coloumn and peaks determined by mass spectrometry in positive and negative modes.The validated method led to tentative identification of eight alkaloids on the basis of their retention times and fragmentation patterns. Results showed that the positive mode response was much higher than the negative ion mode .chromatographic conditions were optimized to obtain high resolution and short run time.

scholarly journals STEROLS AND FLAVONE FROM THE LEAVES OF Vernonia amygdalina Del. GROWING IN THUA THIEN HUE

2018 ◽  
Vol 56 (6) ◽  
pp. 681
Author(s):  
Hoang Le Tuan Anh
Keyword(s):  
Methanol Extract ◽  
2D Nmr ◽  
Vernonia Amygdalina ◽  
Esi Ms ◽  
Chromatographic Methods ◽  
Ms Analysis ◽  
Reported Data ◽  
First Time

By using various chromatographic methods, two sterols and one flavone, vernoniamygda A (1), vernoniamygda B (2), and luteolin (3) were isolated from the methanol extract of the leaves of Vernonia amygdalina. Their structures were determined using 1D, 2D-NMR and ESI-MS analysis as well as by comparison with the reported data. Compounds 1 and 2 were reported from natural for the first time.

scholarly journals PHENYL DERIVATIVES FROM ANTIDESMA HAIANENSIS

2017 ◽  
Vol 55 (1) ◽  
pp. 8
Author(s):  
Le Canh Viet Cuong ◽  
Bui Huu Tai ◽  
Nguyen Xuan Nhiem ◽  
Pham Hai Yen ◽  
Hoang Le Tuan Anh ◽  
...  
Keyword(s):  
Methanol Extract ◽  
2D Nmr ◽  
First Report ◽  
Esi Ms ◽  
Chemical Structures ◽  
Ms Analysis ◽  
Reported Data

Five phenyl derivatives 1-O-(2′,4′ -dihydroxy-6’-methoxyphenyl)-6-O-(4′′-hydroxy-3′′,5′′-dimethoxybenzoyl)-β-D-glucopyranoside (1), 4′-O-[6′′-O-(4′′′-hydroxy-3′′′,5′′′-imethoxybenzoyl) -β-D-glucopyranosyl]-3′-hydroxyphenethyl alcohol (2), 4-hydroxymethyl-2-methoxyphenyl-6′-O-syringoyl-β-D-glucopyranoside (3), phenethyl-6-O-α-L-arabinofuranosyl-β-D-glucopyranoside (4), and 1-O-syringoyl-β-D-glucopyranoside (5) were isolated from the methanol extract of the leaves of the Vietnamese plant Antidesma hainanensis. Their chemical structures were determined using 1D, 2D-NMR and ESI-MS analysis as well as by comparison with reported data. This is the first report of these compounds from A. hainanensis.  

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