scholarly journals Phytochemical Analysis and Anti-Inflammatory and Anti-Osteoarthritic Bioactive Potential of Verbascum thapsus L. (Scrophulariaceae) Leaf Extract Evaluated in Two In Vitro Models of Inflammation and Osteoarthritis

Molecules ◽  
2021 ◽  
Vol 26 (17) ◽  
pp. 5392
Author(s):  
Giovanna Calabrese ◽  
Agata Zappalà ◽  
Anna Dolcimascolo ◽  
Rosaria Acquaviva ◽  
Rosalba Parenti ◽  
...  
Keyword(s):  
Aqueous Extract ◽  
Complex Disease ◽  
Joint Disease ◽  
Total Phenolic ◽  
Phytochemical Analysis ◽  
Phenylethanoid Glycosides ◽  
Verbascum Thapsus ◽  
Leaf Aqueous Extract ◽  
Anti Inflammatory ◽  
Bioactive Potential

Osteoarthritis (OA) is a complex disease, source of pain and disability that affects millions of people worldwide. OA etiology is complex, multifactorial and joint-specific, with genetic, biological and biomechanical components. Recently, several studies have suggested a potential adjuvant role for natural extracts on OA progression, in terms of moderating chondrocyte inflammation and following cartilage injury, thus resulting in an overall improvement of joint pain. In this study, we first analyzed the phenylethanoid glycosides profile and the total amount of polyphenols present in a leaf aqueous extract of Verbascum thapsus L. We then investigated the anti-inflammatory and anti-osteoarthritic bioactive potential of the extract in murine monocyte/macrophage-like cells (RAW 264.7) and in human chondrocyte cells (HC), by gene expression analysis of specifics inflammatory cytokines, pro-inflammatory enzymes and metalloproteases. Six phenylethanoid glycosides were identified and the total phenolic content was 124.0 ± 0.7 mg gallic acid equivalent (GAE)/g of extract. The biological investigation showed that the extract is able to significantly decrease most of the cellular inflammatory markers, compared to both control cells and cells treated with Harpagophytum procumbens (Burch.) DC. ex Meisn, used as a positive control. Verbascum thapsus leaf aqueous extract has the potential to moderate the inflammatory response, representing an innovative possible approach for the inflammatory joint disease treatment.

Phytochemical analysis and anti-inflammatory evaluation of compounds from an aqueous extract of Croton cajucara Benth.

2017 ◽  
Vol 145 ◽  
pp. 821-830 ◽  
Author(s):  
Adamara M. Nascimento ◽  
Daniele Maria-Ferreira ◽  
Fernando T. Dal Lin ◽  
Alexandre Kimura ◽  
Arquimedes P. de Santana-Filho ◽  
...  
Keyword(s):  
Aqueous Extract ◽  
Phytochemical Analysis ◽  
Anti Inflammatory ◽  
Croton Cajucara

scholarly journals A Comparative Study between Plant and Callus Extracts of Abutilon indicum (L.) Sweet: Antioxidant, Antibacterial, Antidiabetic and Anti-Proliferative Activity

2020 ◽  
pp. 13-24
Author(s):  
A. Ayisha Sireen ◽  
J. Anbumalarmathi
Keyword(s):  
Ethyl Acetate ◽  
Aqueous Extract ◽  
Proliferative Activity ◽  
Radical Scavenging Activity ◽  
Radical Scavenging ◽  
Total Phenolic ◽  
Phytochemical Analysis ◽  
Ic50 Value ◽  
Ethyl Acetate Extracts ◽  
Abutilon Indicum

Abutilon indicum is consider to be used in the traditional system of medicine. It is found in tropical and subtropical regions of the world. It is used to treat various diseases. This plant does not cause any side effects to humans. As the plant has wide variety of medicinal properties, the present study aimed to comparative between plant and callus extract of Abutilon indicum (L.) sweet for antioxidant, antibacterial, antidiabetic and anti- proliferative activity. The highest percentage of callus induction (89.50%) and callus weight (1.26 g) was observed in T5 (MS + 2, 4-D (2.5 mg/l) + BAP (2 mg/l) and T8 [IBA (4 mg/l)] respectively. Phytochemical analysis of aqueous and ethyl acetate extracts of A. indicum in vivo plant and in vitro grown callus showed the presence of alkaloids, flavonoids, phenols, carbohydrates, glycosides, protein, terpenoids, saponins, tannins and coumarin. The total phenolic content was high in aqueous extract of callus (30.68 mg TAE/g). Maximum DPPH radical scavenging activity was found in aqueous extract of callus (86%) with IC50 value of 68.49 µg/ml. FT-IR analysis of aqueous extract of A. indicum plant and callus showed the presence of characteristic stretching at 2930.28 and 2927.75 indicating the presence of C-H stretching respectively. GC-MS analysis revealed the presence of 17 compounds in ethyl acetate plant extract, whereas 7 compounds in ethyl acetate callus extract such as tetradecane, 1-chloro, Sulfurous acid 2-prophytridecyl ester and 1- ethyl-3-[2-(octadecylthio) ethyl] thiourea. The ethyl acetate extracts of callus and plant and was found to be effective against Bacillus subtilis (3.1 mm) and Staphylococcus aureus (2.9 mm). Maximum α-amylase inhibitory activity was observed in aqueous callus extract (32.65%) with IC50 value of 833.61 µg/ml. HeLa cell viability was found to be 26.8% and 21.8% in plant and callus extract respectively.

scholarly journals PRELIMINARY PHYTOCHEMICAL SCREENING AND ANTI-INFLAMMATORY EFFECT OF THE AQUEOUS EXTRACT OF TABERNAEMONTANA DIVARICATA FLOWER IN WISTER RATS

2017 ◽  
pp. 9-12
Author(s):  
Deepankar Garga ◽  
Trishna Das
Keyword(s):  
Aqueous Extract ◽  
Inflammatory Activity ◽  
Phytochemical Analysis ◽  
Standard Drug ◽  
Tabernaemontana Divaricata ◽  
Rat Paw Edema ◽  
Oil Extract ◽  
Anti Inflammatory ◽  
Rat Paw ◽  
Inflammatory Effect

Objective: To determine the phytochemical analysis and anti-inflammatory effect of the aqueous extract of Tabernaemontanadivaricata flower in Wister Rats.Methods: The aqueous extract of fresh flowers of the plant Tabernaemontanadivaricata (family-Apocyanaceae) were prepared and analyzed for phytochemical constituents using standard methods. The anti-inflammatory activity of the plant extracts was examined with the help of rat models by taking diclofenac as a standard drug against T. divericata oil extract by using carrageenan induced rat paw edema method with the help of the instrument plethesmometer.Results: The present experiment shows the phytochemical analysis, anti-inflammatory activity of the aqueous extract of fresh flowers of the plant Tabernaemontanadivaricata (family-Apocyanaceae). Various phytochemical analysis revealed the presence of saponins, flavonoids, glycosides, steroids, proteins, phenols, amino acids and tannins. The oil extract of the plant showed much effective anti-inflammatory activity against the standard drug.Conclusion: It can be concluded aqueous extract of the flower of the plant Tabernaemontanadivaricata (family-Apocyanaceae) contain the high presence of phytochemicals. This extract was found to possess promising antimicrobial activity when compared with the standards.

scholarly journals The Nociceptive and Anti-Inflammatory Effects ofArtemisia dracunculusL. Aqueous Extract on Fructose Fed Male Rats

2015 ◽  
Vol 2015 ◽  
pp. 1-5 ◽  
Author(s):  
Shahraki Mohammad Reza ◽  
Mirshekari Hamideh ◽  
Samadi Zahra
Keyword(s):  
Drinking Water ◽  
Aqueous Extract ◽  
Daily Basis ◽  
Male Rats ◽  
Pain Response ◽  
Trial Period ◽  
Pain Scores ◽  
Leaf Aqueous Extract ◽  
Anti Inflammatory ◽  
Post Hoc

Aim & Objective.Artemisia dracunculusL. (Tarragon) species have been used as a traditional medicine. The present study was designed to evaluate the nociceptive and anti-inflammatory effects ofA. dracunculusL. leaf aqueous extract on fructose drinking water (FDW) in male rats.Materials & Methods. Forty-eight Wistar-albino male rats weighing 200–250 g were divided into control (C), control extract (CE), FDW, and FDWE groups (n=12). Group C did not receive any agents; Group CE did 100 mg/kgA. dracunculusL. aqueous extract on a daily basis for duration of four weeks. FDW Group received fructose drinking water (10%, weight/volume) but did not receive any agents during trial period. FDWE group received 100 mg/kgA. dracunculusL. aqueous extract during trial period. At the end of experiment, a biphasic pain response was induced following interplanetary injection of formalin (50 µL, 1%). Obtained data were analyzed using SPSS software version 17 and using ANOVA and Tukey post hoc tests. Results were expressed as mean ± SE. Statistical differences were considered significant atP<0.05.Results. Our findings revealed that acute and chronic pain scores in FDW group are significantly higher than other ones andA. dracunculusL. aqueous extract causes significant decreasing of this parameter in FDWE group (P<0.001). Moreover, IL6 and TNF values in this group were significantly decreased compared to FDW group (P<0.05).Conclusion. Results in the present study show that FDW causes the pain response score to increase and cause proinflammatory cytokines in rat model butA. dracunculusL. leaf aqueous extract improves values of these parameters.

scholarly journals Computational and Pharmacological Studies on the Antioxidant, Thrombolytic, Anti-Inflammatory, and Analgesic Activity of Molineria capitulata

2021 ◽  
Vol 43 (2) ◽  
pp. 434-456
Author(s):  
Mohammad Ashiqur Rahman Bhuiyan Shovo ◽  
Marzia Rahman Tona ◽  
Jannatul Mouah ◽  
Fayza Islam ◽  
Md. Helal Uddin Chowdhury ◽  
...  
Keyword(s):  
Computational Models ◽  
Protein Denaturation ◽  
Computational Study ◽  
Reducing Power ◽  
Total Phenolic ◽  
Phytochemical Analysis ◽  
Anti Inflammatory ◽  
Analgesic Activities ◽  
Reduction Capacity ◽  
Pain Inhibition

Molineria capitulata is an ornamental plant that has traditionally been used to treat several chronic diseases. The present study was designed to examine the antioxidant, cytotoxic, thrombolytic, anti-inflammatory, and analgesic activities of a methanolic extract of M. capitulata leaves (MEMC) using both experimental and computational models. Previously established protocols were used to perform qualitative and quantitative phytochemical screening in MEMC. A computational study, including molecular docking and ADME/T analyses, was performed. The quantitative phytochemical analysis revealed the total phenolic and flavonoid contents as 148.67 and 24 mg/g, respectively. Antioxidant activity was assessed by examining the reducing power of MEMC, resulting in absorbance of 1.87 at 400 µg/mL, demonstrating a strong reduction capacity. The extract exhibited significant protection against blood clotting and showed the highest protein denaturation inhibition at 500 µg/mL. In both the acetic acid-induced writhing and formalin-induced paw-licking models, MEMC resulted in significant potential pain inhibition in mice. In the computational analysis, 4-hydroxybenzaldehyde, orcinol glucoside, curcapital, crassifogenin C, and 2,6-dimethoxy-benzoic acid displayed a strong predictive binding affinity against the respective receptors. These findings indicated that M. capitulata possesses significant pharmacological activities to an extent supported by computational studies.

scholarly journals Evaluation of the curative role, Anti-inflammatory and anti-oxidative activity of some dietary spices on carrageenan induced paw edema in albino Wistar rats

2020 ◽  
Vol 10 (5-s) ◽  
pp. 90-96
Author(s):  
Khaoula Bouaoud ◽  
Noreddine Menadi ◽  
Mohammed Zairi ◽  
Sofiane Bouazza ◽  
Hadjer Bekhadda ◽  
...  
Keyword(s):  
Aqueous Extract ◽  
Wistar Rats ◽  
Red Pepper ◽  
Total Phenolic ◽  
Control Group ◽  
Paw Edema ◽  
Standard Drug ◽  
Reference Drug ◽  
Anti Inflammatory ◽  
Dietary Spices

Background: Inflammation is a major burning problem worldwide and billions of individuals are affected. Some of the spices used daily in Algerian cuisine have been known to possess major anti-inflammatory effects. Turmeric, red pepper, ginger, cinnamon and cumin  etc. are a few of the wide spectrum of spices used in Algeria. The aim of the study was to investigate the antiinflammatory effect  and anti actvity of some dietary spices in carrageenan induced models of inflammation on Wistar  rats. Methods: Albino wistar rats were divided into four groups. Inflammation was induced on the animal by injecting the right hand paw with carrageenan (0.1 ml of 1%). Group 1  was fed with food and water and  treated with carrageenan (control) whereas group 2 and  3 treated with different doses (100 and 200 mg/kg/bw) of aqueous extract of some dietary spices with carrageenan, respectively. Group 4  treated with standard drug diclofenac sodium (10 mg/kg b.w., reference drug for inflammation), once for 24 hrs. Results: The content of total phenolic was 137.2±21.6 mg/g and flavonoids 122.3±5.8 mg/g. After 60, 120, 180, 240 and 300 min, the data indicate that  some dietary spices 100 and 200 mg/kg was significantly effective reducing paw edema volumes induced by carrageenan compared to control (p <0.01). The levels of serum pro-inflammatory white blood cells, neutrophile, lymphocyte, monocyte,  eosinophile, total protein and protein c reactive and total cephalin KADIN  and platelts in the control group were significantly increased by carrageenan injection compared with the normal group. The rats pretreated with some spices 100 and 200 mg/kg had significantly lower levels of pro-inflammatory to compare control group. The paw edema in carrageenan-induced rats was considerably reduced by treating with 100 and 200  mg/kg aqueous extract of some dietary spices when compared to the untreated  rats (p<0.001). Conclusion : The present results clearly demonstarted that carrageenan significantly increased paw edema whereas some dietary treated rats significantly decreased the paw edema. The study underlines the importance of some of the frequently used spices (Turmeric, red pepper, ginger, cinnamon and cumin)  in the treatment of inflammation.

scholarly journals Interactive Effects of Light and Melatonin on Biosynthesis of Silymarin and Anti-inflammatory Potential in Callus Cultures of Silybum marianum L.

2019 ◽  
Author(s):  
Muzamil Shah ◽  
Muhammad Asad Ullah ◽  
Samantha Drouet ◽  
Muhammad Younas ◽  
Duangjai Tungmunnithum ◽  
...  
Keyword(s):  
Continuous Light ◽  
Biomass Accumulation ◽  
Interactive Effects ◽  
Total Phenolic ◽  
Naphthalene Acetic Acid ◽  
Phytochemical Analysis ◽  
Silybum Marianum ◽  
Light Regimes ◽  
Anti Inflammatory

Silybum marianum L. is a well-known medicinal herb, primarily used in liver protection. Light strongly affects several physiological processes along with secondary metabolites biosynthesis in plants. Herein, S. marianum was exploited for in vitro potential under different light regimes in the presence of melatonin. The optimum callogenic response occurred in combination of 1.0 mg/L α-naphthalene acetic acid and 0.5 mg/L 6-Benzylaminopurine under photoperiod. Continuous light associated with melatonin treatment increased total flavonoid content (TFC), total phenolic content (TPC) and antioxidant potential, followed by photoperiod and dark treatments. The increased level of melatonin has a synergistic effect on biomass accumulation under continuous light and photoperiod, while adverse effect was observed under dark condition. More detailed phytochemical analysis showed maximum total silymarin content (11.92 mg/g DW) when placed under continuous light + 1.0 mg/L melatonin. Individually, the level of silybins (A and B), silydianin, isolsilychristin and silychristin was found highest under continuous light. Anti-inflammatory activities were also studied and highest percent inhibition was recorded against 15-LOX for cultures cultivated under continuous light (42.33%). The current study helps to better understand the influence of melatonin and different light regimes on silymarin production as well as antioxidant and anti-inflammatory activities in S. marianum callus extracts.

scholarly journals Local anti-inflammatory activity: Topical formulation containing Kalanchoe brasiliensis and Kalanchoe pinnata leaf aqueous extract

2019 ◽  
Vol 113 ◽  
pp. 108721 ◽  
Author(s):  
Edilane Rodrigues Dantas de Araújo ◽  
Juliana Félix-Silva ◽  
Jacinthia Beatriz Xavier-Santos ◽  
Júlia Morais Fernandes ◽  
Gerlane Coellho Bernardo Guerra ◽  
...  
Keyword(s):  
Aqueous Extract ◽  
Inflammatory Activity ◽  
Topical Formulation ◽  
Leaf Aqueous Extract ◽  
Kalanchoe Pinnata ◽  
Anti Inflammatory

scholarly journals In-vitro antioxidant, anti-cancer, and anti-inflammatory activities of selected medicinal plants from western Nepal

2020 ◽  
Vol 6 (1) ◽  
Author(s):  
Bishnu Prasad Pandey ◽  
Kapil Adhikari ◽  
Suman Prakash Pradhan ◽  
Hee Jeong Shin ◽  
Eun Kyeong Lee ◽  
...  
Keyword(s):  
Medicinal Plants ◽  
Cell Line ◽  
Cell Lines ◽  
Cancer Cell ◽  
Antioxidant Properties ◽  
Melia Azedarach ◽  
Total Phenolic ◽  
Phytochemical Analysis ◽  
Flavonoid Content ◽  
Anti Inflammatory

Abstract Background This study was focused on the measurement of anticancer properties of six medicinal plants from western Nepal in three cell lines; HeLa, Hep3B, and HCT116, and anti-inflammatory properties in RAW 264.7 cell line through NO, PGE2, and TNF-α production. In addition, the phytochemical screening, total phenolic, flavonoid content, and antioxidant properties were evaluated. Results The qualitative phytochemical analysis revealed the presence of different secondary metabolite and range of total phenolic and total flavonoid content. The highest antioxidant activities were observed in Bergenia pacumbis against both DPPH (IC50 = 25.97 ± 0.19 μg/mL) and ABTS (IC50 = 14.49 ± 0.40 μg/mL). Furthermore, the highest antiproliferative effect against cervical, liver, and colon cancer cell lines were observed in Melia azedarach as IC50 values of 10.50, 5.30, and 1.57 μg/mL respectively, while the strongest anti-metastatic potential on liver cancer cell line was found in Pleurospermum benthamii. In addition, P. benthamii showed the most potent anti-inflammatory effect in RAW264.7 murine macrophage cells. Conclusion This study provided the evidence for M. azedarach and P. benthamii to have great anticancer potential and finding builds the enough scientific backgrounds in future to isolate and purify the bioactive compounds for further applications.

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