scholarly journals A Neoglycoprotein-Immobilized Fluorescent Magnetic Bead Suspension Multiplex Array for Galectin-Binding Studies

Molecules ◽  
2021 ◽  
Vol 26 (20) ◽  
pp. 6194
Author(s):  
Libo Zhang ◽  
Hai Yu ◽  
Yuanyuan Bai ◽  
Bijoyananda Mishra ◽  
Xiaoxiao Yang ◽  
...  
Keyword(s):  
Magnetic Beads ◽  
Magnetic Bead ◽  
Binding Studies ◽  
Binding Properties ◽  
Plant Lectins ◽  
Protein Conjugates ◽  
Binding Preference ◽  
Galectin 3 ◽  
Multiplex Array ◽  
Bead Suspension

Carbohydrate-protein conjugates have diverse applications. They have been used clinically as vaccines against bacterial infection and have been developed for high-throughput assays to elucidate the ligand specificities of glycan-binding proteins (GBPs) and antibodies. Here, we report an effective process that combines highly efficient chemoenzymatic synthesis of carbohydrates, production of carbohydrate-bovine serum albumin (glycan-BSA) conjugates using a squarate linker, and convenient immobilization of the resulting neoglycoproteins on carboxylate-coated fluorescent magnetic beads for the development of a suspension multiplex array platform. A glycan-BSA-bead array containing BSA and 50 glycan-BSA conjugates with tuned glycan valency was generated. The binding profiles of six plant lectins with binding preference towards Gal and/or GalNAc, as well as human galectin-3 and galectin-8, were readily obtained. Our results provide useful information to understand the multivalent glycan-binding properties of human galectins. The neoglycoprotein-immobilized fluorescent magnetic bead suspension multiplex array is a robust and flexible platform for rapid analysis of glycan and GBP interactions and will find broad applications.

The Research of a New Method for Manipulating Magnetic Beads through Multi-Layered Flat Micro-Coils Coupled with Permanent Magnet

2013 ◽  
Vol 753-755 ◽  
pp. 1571-1575
Author(s):  
Zhi Hua Liu ◽  
Yu Feng Huang ◽  
Jian Peng Li ◽  
Xin Wei Xu
Keyword(s):  
Magnetic Field ◽  
Permanent Magnet ◽  
Flow Velocity ◽  
Magnetic Beads ◽  
Magnetic Bead ◽  
New Method ◽  
Viscous Resistance ◽  
Main Factors ◽  
The Magnetic Field

Magnetic bead droplet's non-contacted manipulation can be realized in Electromagnetic MEMS, but how to achieve magnetic beads manipulation is the major problem. A new method of multi-layered flat coils coupled with permanent magnet was proposed. Firstly, the theory of magnetic bead manipulation was analyzed and the main factors affected the magnetic beads manipulation was identified; then the magnetic field of multi-layered flat coils and Stokes viscous resistance of magnetic beads were analyzed and simulated quantificationally; finally the magnetic bead capture area was got under different flow velocity. Consequently the feasibility and correctness of this method was verified.

scholarly journals The Magnetic Bead Computing Model of the 0-1 Integer Programming Problem Based on DNA Cycle Hybridization

2021 ◽  
Vol 2021 ◽  
pp. 1-7
Author(s):  
Rujie Xu ◽  
Zhixiang Yin ◽  
Zhen Tang ◽  
Jing Yang ◽  
Jianzhong Cui ◽  
...  
Keyword(s):  
Integer Programming ◽  
Programming Problem ◽  
Magnetic Beads ◽  
Specific Binding ◽  
Optimal Solution ◽  
High Sensitivity ◽  
Magnetic Bead ◽  
Integer Programming Problem ◽  
Computing Model ◽  
Dna Strands

Magnetic beads and magnetic Raman technology substrates have good magnetic response ability and surface-enhanced Raman technology (SERS) activity. Therefore, magnetic beads exhibit high sensitivity in SERS detection. In this paper, DNA cycle hybridization and magnetic bead models are combined to solve 0-1 integer programming problems. First, the model maps the variables to DNA strands with hairpin structures and weights them by the number of hairpin DNA strands. This result can be displayed by the specific binding of streptavidin and biotin. Second, the constraint condition of the 0-1 integer programming problem can be accomplished by detecting the signal intensity of the biological barcode to find the optimal solution. Finally, this model can be used to solve the general 0-1 integer programming problem and has more extensive applications than the previous DNA computing model.

Rapid detection of Clostridium difficile via magnetic bead aggregation in cost-effective polyester microdevices with cell phone image analysis

The Analyst ◽  
2016 ◽  
Vol 141 (19) ◽  
pp. 5637-5645 ◽  
Author(s):  
Jacquelyn A. DuVall ◽  
Scott T. Cabaniss ◽  
Morgan L. Angotti ◽  
John H. Moore ◽  
Mayuresh Abhyankar ◽  
...  
Keyword(s):  
Image Analysis ◽  
Clostridium Difficile ◽  
Rapid Detection ◽  
Cell Phone ◽  
Magnetic Beads ◽  
Cost Effective ◽  
Magnetic Bead ◽  
Isothermal Amplification ◽  
Specific Detection

A centrifugally-driven polyester microdevice for sequence-specific detection ofClostridium difficileusing magnetic beads, isothermal amplification, and cell phone image analysis.

scholarly journals Integrated Analyses of Proteins and Their Glycans in a Magnetic Bead–Based Multiplex Assay Format

2013 ◽  
Vol 59 (1) ◽  
pp. 315-324 ◽  
Author(s):  
Danni Li ◽  
Hanching Chiu ◽  
Jing Chen ◽  
Hui Zhang ◽  
Daniel W Chan
Keyword(s):  
Prostate Cancer ◽  
Magnetic Beads ◽  
Biomarker Discovery ◽  
Magnetic Bead ◽  
Protein Quantification ◽  
Integrated System ◽  
Clinical Samples ◽  
Improved Performance ◽  
Integrated Detection ◽  
Cancer Tissues

BACKGROUND Well-annotated clinical samples are valuable resources for biomarker discovery and validation. Multiplex and integrated methods that simultaneously measure multiple analytes and generate integrated information about these analytes from a single measurement are desirable because these methods help conserve precious samples. We developed a magnetic bead–based system for multiplex and integrated glycoprotein quantification by immunoassays and glycan detection by lectin immunosorbent assays (LISAs). METHODS Magnetic beads coupled with antibodies were used for capturing proteins of interest. Biotinylated antibodies in combination with streptavidin-labeled phycoerythrin were used for protein quantification. In the LISAs, biotinylated detection antibodies were replaced by biotinylated lectins for glycan detection. RESULTS Using tissue inhibitor of metallopeptidase 1 (TIMP-1), tissue plasminogen activator, membrane metallo-endopeptidase, and dipeptidyl peptidase-IV (DPP-4) as models, we found that the multiplex integrated system was comparable to single immunoassays in protein quantification and LISAs in glycan detection. The merits of this system were demonstrated when applied to well-annotated prostate cancer tissues for validation of biomarkers in aggressive prostate cancer. Because of the system's multiplex ability, we used only 300 ng of tissue protein for the integrated detection of glycans in these proteins. Fucosylated TIMP-1 and DPP-4 offered improved performance over the proteins in distinguishing aggressive and nonaggressive prostate cancer. CONCLUSIONS The multiplex and integrated system conserves samples and is a useful tool for validation of glycoproteins and their glycoforms as biomarkers.

Magnetic Spin Valve Sensors with Different Geometry to Magnetic Bead Detection for Biosensor Application

2007 ◽  
Vol 1032 ◽  
Author(s):  
Jeong Dae Suh ◽  
Myung Ae Chung
Keyword(s):  
Magnetic Beads ◽  
Spin Valve ◽  
Magnetic Bead ◽  
Highly Sensitive ◽  
Magnetic Spin ◽  
Biosensor Application ◽  
Detection Of Biomolecules ◽  
Meander Line ◽  
Cross Type ◽  
Line Type

AbstractWe have demonstrated the use of highly sensitive spin valve sensors for the detection of micron magnetic beads. By using a ring type, cross type, and meander line type sensors, we were able to detect the presence of 2.8 μm size magnetic beads in real time by direct measurement of magnetic dipole fields from magnetic beads. The sensitivity of the ring, cross and meander line sensors were obtained about 50 μV/Oe, 7 μV/Oe, 30 μV/Oe and sensor output signals of 50 μV , 30 μV, 90 μV were obtained in an external applied field of 10 Oe and 1 mA sense current. Our results shows that ring, cross, and meander line shape spin valve sensors are very promising candidates for the detection of biomolecules with magnetic labels.

scholarly journals Gate-Controlled Microfluidic Chamber With Magnetic Bead for DNA Sequencing-by-Synthesis Technology

2007 ◽  
Author(s):  
Hesaam Esfandyarpour ◽  
Ronald W. Davis
Keyword(s):  
Dna Sequencing ◽  
Magnetic Beads ◽  
Magnetic Bead ◽  
Synthesis Methods ◽  
Dna Polymerization ◽  
Microfluidic Chamber ◽  
Pdms Channel ◽  
Potential Applications ◽  
Sequencing By Synthesis ◽  
And Control

In this paper we present a novel microfluidic platform for DNA sequencing-by-synthesis methods (e.g. pyrosequencing). The proposed platform is based on the valve-controllable PDMS channel technology with DNA-coated magnetic beads. The encapsulation of the reaction of DNA polymerization in picoliter-sized wells provides for excellent isolation and control for detection. This separation prevents cross-talk amongst neighbor reactors which is one of the most limitations for higher integration of the current technologies. Through application of an external magnetic field the beads can be allocated with better accuracy. In addition this property can help mixing for the reaction. The proposed system is useful for a number of other bio-species detection and sorting templates. This paper illustrates the design and experimental results of a primary template as well as different advantages and potential applications of the Gate-Controlled Magnetic Bead (GCMB) platform in the world of DNA sequencing and genetics.

scholarly journals Galectin-3 as a Potential Target to Prevent Cancer Metastasis

2015 ◽  
Vol 9 ◽  
pp. CMO.S29462 ◽  
Author(s):  
Hafiz Ahmed ◽  
Dina M. M. Alsadek
Keyword(s):  
Cancer Progression ◽  
Growth Regulation ◽  
Cancer Metastasis ◽  
Carbohydrate Binding ◽  
Biological Processes ◽  
Binding Properties ◽  
Galectin 3 ◽  
Carbohydrate Ligands ◽  
Lectin Family ◽  
Binding Lectin

Interactions between two cells or between cell and extracellular matrix mediated by protein–carbohydrate interactions play pivotal roles in modulating various biological processes such as growth regulation, immune function, cancer metastasis, and apoptosis. Galectin-3, a member of the β-galactoside-binding lectin family, is involved in fibrosis as well as cancer progression and metastasis, but the detailed mechanisms of its functions remain elusive. This review discusses its structure, carbohydrate-binding properties, and involvement in various aspects of tumorigenesis and some potential carbohydrate ligands that are currently investigated to block galectin-3 activity.

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