scholarly journals Discovery of Three New Monoterpenoid Indole Alkaloids from the Leaves of Gardneria multiflora and Their Vasorelaxant and AChE Inhibitory Activities

Molecules ◽  
2021 ◽  
Vol 26 (23) ◽  
pp. 7191
Author(s):  
Sheng-Yuan Zhang ◽  
Zi-Wei Li ◽  
Jie Xu ◽  
Qiu-Ling Chen ◽  
Min Song ◽  
...  
Keyword(s):  
Circular Dichroism ◽  
Indole Alkaloids ◽  
2D Nmr ◽  
Spectroscopic Methods ◽  
Monoterpenoid Indole Alkaloids ◽  
Ic50 Values ◽  
Vasorelaxant Activity ◽  
Inhibitory Activities ◽  
Circular Dichroïsm

Three novel monoterpenoid indole alkaloids gardflorine A (1), gardflorine B (2), and gardflorine C (3) were isolated from the leaves of Gardneria multiflora. Their structures, including absolute configurations, were established on the basis of spectroscopic methods (MS, UV, IR, 1D and 2D NMR) and circular dichroism experiments. All the compounds were evaluated for their vasorelaxant and acetylcholinesterase (AChE) inhibitory activities. Compound 1 exhibited potent vasorelaxant activity, with an EC50 value of 8.7 μM, and compounds 2 and 3 showed moderate acetylcholinesterase (AChE) inhibitory activities, with IC50 values of 26.8 and 29.2 μM, respectively.

scholarly journals A New Neolignan from Panicum turgidum

2016 ◽  
Vol 11 (7) ◽  
pp. 1934578X1601100
Author(s):  
Ahmed A. Zaki ◽  
Zulfiqar Ali ◽  
Yasser A. El-Amier ◽  
Ikhlas A. Khan
Keyword(s):  
Circular Dichroism ◽  
Optical Rotation ◽  
2D Nmr ◽  
Circular Dichroism Spectroscopy ◽  
Spectroscopic Methods ◽  
Relative Configuration ◽  
Esi Ms ◽  
Spectroscopy Optical ◽  
Circular Dichroïsm

A new neolignan, paniculignan (1), and a known lignan, tetracentronside B (2), were isolated from Panicum turgidum. The structure of 1 was elucidated by extensive spectroscopic methods including 1D and 2D NMR and HR-ESI-MS. The relative configuration of 1 was determined on the basis of circular dichroism spectroscopy, optical rotation, and NOESY correlations.

scholarly journals Induction of Secondary Metabolites from the Marine-Derived Fungus Aspergillus versicolor through Co-cultivation with Bacillus subtilis

Planta Medica ◽  
2019 ◽  
Vol 85 (06) ◽  
pp. 503-512 ◽  
Author(s):  
Nada Abdel-Wahab ◽  
Sebastian Scharf ◽  
Ferhat Özkaya ◽  
Tibor Kurtán ◽  
Attila Mándi ◽  
...  
Keyword(s):  
Bacillus Subtilis ◽  
Circular Dichroism ◽  
Density Functional ◽  
2D Nmr ◽  
Fungal Culture ◽  
Aspergillus Versicolor ◽  
Electronic Circular Dichroism ◽  
Ic50 Values ◽  
Culture Extract ◽  
Circular Dichroïsm

AbstractA new cyclic pentapeptide, cotteslosin C (1), a new aflaquinolone, 22-epi-aflaquinolone B (3), and two new anthraquinones (9 and 10), along with thirty known compounds (2, 4 – 8, 11 – 34) were isolated from a co-culture of the sponge-associated fungus Aspergillus versicolor with Bacillus subtilis. The new metabolites were only detected in the co-culture extract, but not when the fungus was grown under axenic conditions. Furthermore, the co-culture extract exhibited an enhanced accumulation of the known constituents versicolorin B (14), averufin (16), and sterigmatocyctin (19) by factors of 1.5, 2.0, and 4.7, respectively, compared to the axenic fungal culture. The structures of the isolated compounds were elucidated on the basis of 1D and 2D NMR spectra and mass spectrometry as well as by comparison with literature data. The absolute configuration of compounds 3, 9, and 10 was determined by ECD (electronic circular dichroism) analysis aided by TDDFT-ECD (time-dependent density functional theory electronic circular dichroism) calculations. Compounds 15, 18 – 21, and 26 exhibited strong to moderate cytotoxic activity against the mouse lymphoma cell line L5178Y, with IC50 values ranging from 2.0 to 21.2 µM, while compounds 14, 16, 31, 32, and 33 displayed moderate inhibitory activities against several gram-positive bacteria, with MIC values ranging from 12.5 to 50 µM.

scholarly journals Chemical Constituents of Euonymus glabra

2015 ◽  
Vol 10 (12) ◽  
pp. 1934578X1501001
Author(s):  
Jie Ren ◽  
Yang-Guo Xie ◽  
Xing Wang ◽  
Shi-Kai Yan ◽  
Hui-Zi Jin ◽  
...  
Keyword(s):  
Circular Dichroism ◽  
Phenolic Compound ◽  
Chemical Constituents ◽  
No Production ◽  
Spectroscopic Methods ◽  
Inhibitory Effects ◽  
Raw264.7 Macrophages ◽  
Circular Dichroism Analysis ◽  
Inhibitory Activities ◽  
Circular Dichroïsm

One new phenolic compound (1) and one new flavan (2), together with eight known compounds (3–10) were isolated from the stems and twigs of Euonymus glabra Roxb. Their structures were elucidated mainly on the basis of 1D and 2D spectroscopic methods and circular dichroism analysis. In addition, compounds 1–10 were tested for their inhibitory effects against LPS-induced NO production in RAW264.7 macrophages. Compounds 1–5 and 7 exhibited moderate inhibitory activities with IC50 values ranged from 5.1 to 11.9 μM.

Isolation of New Glycosides from Anemarrhena Asphodeloides Rhizome and Screening of their Anticancer Activity

2019 ◽  
Vol 16 (6) ◽  
pp. 474-477 ◽  
Author(s):  
Pham Van Khang ◽  
Nguyen Thi Hien Lan ◽  
Le Quang Truong ◽  
Mai Thi Minh Chau ◽  
Mai Xuan Truong ◽  
...  
Keyword(s):  
Anticancer Activity ◽  
Spectral Data ◽  
Cell Lines ◽  
Cancer Cell ◽  
Spectroscopic Analysis ◽  
2D Nmr ◽  
Ic50 Values ◽  
Nmr Techniques ◽  
Inhibitory Activities ◽  
Anemarrhena Asphodeloides

In this report, two new steroidal glycosides were isolated and determined from n-butanol fraction of A.asphodeloides. The structures were confirmed in comparison with the spectral data of known compounds by using different spectroscopic analysis approaches including 1D & 2D-NMR techniques and HRMS. The anti-proliferation screening against cancer cell lines A549 and HeLa indicated that compound 1 exhibited good inhibitory activities with IC50 values of 0.79 and 0.55 µg/mL, respectively.

Chemical Constituents from Chloranthus elatior and Their Inhibitory Effect on Human Dihydroorotate Dehydrogenase

Planta Medica ◽  
2021 ◽  
Author(s):  
Qian Yang ◽  
An Jia ◽  
Xizi Liu ◽  
Shiyi Han ◽  
Siyang Fan
Keyword(s):  
Circular Dichroism ◽  
Chemical Constituents ◽  
Inhibitory Effect ◽  
Mass Spectrometric ◽  
Dihydroorotate Dehydrogenase ◽  
Active Compounds ◽  
Electronic Circular Dichroism ◽  
Aerial Parts ◽  
Ic50 Values ◽  
Circular Dichroïsm

AbstractA new sesquiterpene, chlorantholide G (1), a new sesquiterpene dimer, elatiolactone (2), and 2 new diterpenes, elatiorlabdane B (3) and elatiorlabdane C (4), together with 51 known compounds, were isolated from the aerial parts of Chloranthus elatior. The new structures including their absolute configurations were mainly established by mass spectrometric, NMR, and electronic circular dichroism experiments. All isolated compounds were tested for their anti-hDHODH activity. (4S,6R)-4-hydroxy-6-isopropyl-3-methylcyclohex-2-enone (5) and (4S,5R,9S,10R)-8(17),12,14-labdatrien-18-oic acid (29) were the most active compounds with IC50 values of 18.7 and 30.7 µM, respectively.

scholarly journals Fusarins G–L with Inhibition of NO in RAW264.7 from Marine-Derived Fungus Fusarium solani 7227

Marine Drugs ◽  
2021 ◽  
Vol 19 (6) ◽  
pp. 305
Author(s):  
Guangyuan Luo ◽  
Li Zheng ◽  
Qilin Wu ◽  
Senhua Chen ◽  
Jing Li ◽  
...  
Keyword(s):  
Fusarium Solani ◽  
2D Nmr ◽  
Inflammatory Activity ◽  
Raw264.7 Cells ◽  
Spectroscopic Methods ◽  
X Ray ◽  
X Ray Crystallography ◽  
Structure Activity ◽  
Ic50 Values ◽  
New Compounds

Six new fusarin derivatives, fusarins G–L (1–6), together with five known compounds (5–11) were isolated from the marine-derived fungus Fusarium solani 7227. The structures of the new compounds were elucidated by means of comprehensive spectroscopic methods (1D and 2D NMR, HRESIMS, ECD, and ORC) and X-ray crystallography. Compounds 5–11 exhibited potent anti-inflammatory activity by inhibiting the production of NO in RAW264.7 cells activated by lipopolysaccharide, with IC50 values ranging from 3.6 to 32.2 μM. The structure–activity relationships of the fusarins are discussed herein.

scholarly journals Bioactivity-Guided Isolation and Identification of New and Immunosuppressive Monoterpenoid Indole Alkaloids from Rauvolfia yunnanensis Tsiang

Molecules ◽  
2019 ◽  
Vol 24 (24) ◽  
pp. 4574 ◽  
Author(s):  
Li-Mei Li ◽  
Shun-Dong Shi ◽  
Yang Liu ◽  
Qiang Zou
Keyword(s):  
Cell Proliferation ◽  
T Cell ◽  
Indole Alkaloids ◽  
T Cell Proliferation ◽  
Ionization Mass ◽  
Immunosuppressive Activity ◽  
Isolation And Identification ◽  
Monoterpenoid Indole Alkaloids ◽  
Total Alkaloids ◽  
Ic50 Values

Three new 11-hydroxyburnamine (1) and rauvoyunnanines A–B (2–3), and fourteen known (4–17) monoterpenoid indole alkaloids were isolated from the total alkaloids extract of Rauvolfia yunnanensis, which exhibited promising immunosuppressive activity on T cell proliferation in preliminary screening. Their structures were determined by analysis of high-resolution electrospray ionization mass (HRESIMS), ultraviolet (UV) and nuclear magnetic resonance (NMR) data, and by comparison with the literature. All the alkaloids were evaluated for inhibitory activity on T cell proliferation. Among them, one new compound (1) and reserpine (6) exhibited moderate immunosuppressive activity, with IC50 values of 5.9 μM and 5.0 μM, respectively.

scholarly journals Two New Spiro-Heterocyclic γ-Lactams from A Marine-Derived Aspergillus fumigatus Strain CUGBMF170049

Marine Drugs ◽  
2019 ◽  
Vol 17 (5) ◽  
pp. 289 ◽  
Author(s):  
Xiuli Xu ◽  
Jiahui Han ◽  
Yanan Wang ◽  
Rui Lin ◽  
Haijin Yang ◽  
...  
Keyword(s):  
Spectral Analysis ◽  
Circular Dichroism ◽  
Aspergillus Fumigatus ◽  
2D Nmr ◽  
Two Dimensional ◽  
One Dimensional ◽  
Helvolic Acid ◽  
Circular Dichroïsm

Two new spiro-heterocyclic γ-lactam derivatives, cephalimysins M (1) and N (2), were isolated from the fermentation cultures of the marine-derived fungus Aspergillus fumigatus CUGBMF17018. Two known analogues, pseurotin A (3) and FD-838 (4), as well as four previously reported helvolic acid derivatives, 16-O-propionyl-16-O-deacetylhelvolic acid (5), 6-O-propionyl-6-O-deacetylhelvolic acid (6), helvolic acid (7), and 1,2-dihydrohelvolic acid (8) were also identified. One-dimensional (1D), two-dimensional (2D) NMR, HRMS, and circular dichroism spectral analysis characterized the structures of the isolated compounds.

Pseurotin A1 and A2, two new 1-oxa-7-azaspiro[4.4]non-2-ene-4,6-diones from the holothurian-derived fungus Aspergillus fumigatus WFZ-25

2011 ◽  
Vol 89 (1) ◽  
pp. 72-76 ◽  
Author(s):  
Fa-Zuo Wang ◽  
De-Hai Li ◽  
Tian-Jiao Zhu ◽  
Min Zhang ◽  
Qian-Qun Gu
Keyword(s):  
Circular Dichroism ◽  
Aspergillus Fumigatus ◽  
Spectroscopic Data ◽  
2D Nmr ◽  
Esi Ms ◽  
Extensive Analysis ◽  
Circular Dichroïsm ◽  
New Metabolites

Two new metabolites containing a 1-oxa-7-azaspiro[4.4]non-2-ene-4,6-dione core, pseurotin A1 (1) and A2 (2), as well as pseurotin A (3), were isolated from the holothurian-derived fungus Aspergillus fumigatus WFZ-25. These compounds were determined using extensive analysis of their spectroscopic data, including 1D and 2D NMR, HR-ESI-MS, and circular dichroism (CD) experiments.

Sign in / Sign up

Export Citation Format

Share Document