scholarly journals Impedance Measures and a Mounting Technique for Drosophila: Larval Movements, Heart Rate, Imaging, and Electrophysiology

2020 ◽  
Vol 3 (1) ◽  
pp. 12
Author(s):  
Noah de Castro ◽  
Robin Lewis Cooper

Monitoring movements of larval Drosophila with electrical detection allows one to record the behaviors without the use of lights and cameras. This is a suitable technique when studying the use of light-sensitive proteins in optogenetic studies. Electrical measures are feasible to use in determining when a larva starts to move or continues to move after a light induced activation of channelrhodopsin. We have developed a technique using an electrical measure of the media as an index of larval movement. As a proof of concept, recordings with an infrared camera of the larval movement were simultaneous made with electrical measures. The two techniques parallel each other in their ability to index larval movements. Bright light-emitting diode (LED) lights used in optogenetic experiments tend to saturate the detectors of the camera unless filters are used and different filters maybe necessary depending on the LED spectrum and sensitivity of the camera. Impedance measures are independent of the type of LED or brightness. We also assessed the use of a non-solvent based glue (3M Vetbond) to hold larvae in place while measuring synaptic function of neuromuscular junctions, cardiac function and influence of modulators, or activation of light-sensitive channels.

2011 ◽  
Vol 2011 (1) ◽  
pp. 000117-000122
Author(s):  
Donald J. Beck ◽  
Jessica Sylvester

Since the introduction of automated die and wire bonders in the 1980s, equipment manufacturers and process engineers have been challenged to balance speed with repeatability. Today, die bonders can perform epoxy die attach at a rate of 1.5 to 4 thousand die per hour [6]; and wire bonders can interconnect complex packages at speeds of more than 10 wires per second [7]. The advantage of automation is speed and consistency—however, there is one major concern with operating at these speeds: if something in the assembly process is wrong, everything will be wrong. Having tightly regulated assembly processes helps avoid the risk of building a large batch of rejected product. This paper presents a methodology and process flow supporting High Bright Light Emitting Diode (HB LED) automated assembly, supported by equipment certification, product inspection and SPC data collection methods. The methods presented in this paper have been formulated through extensive work in the high-reliability microelectronics industry and commercial production lines over the last three decades. To ensure time-to-market success in high-volume production, specific methods to achieve throughput and quality are required. This paper will cover the strategies and methods necessary to achieve the ultimate goal of an automated precision HB LED assembly—to blend the requirements of high-reliability and high-throughput to support high-volume commercial production.


Plants ◽  
2020 ◽  
Vol 9 (10) ◽  
pp. 1351
Author(s):  
Chia-Chen Chen ◽  
Maw-Rong Lee ◽  
Chi-Rei Wu ◽  
Hsin-Ju Ke ◽  
Hui-Min Xie ◽  
...  

Investigations were carried out to study the effects of light-emitting diode (LED) lights on growth and development of isosteroidal alkaloids in embryogenic calli of Fritillaria cirrhosa D. Don, an important traditional Chinese medicine herb. Calli were cultured in glass bottles, each containing 100 mL of Murashige and Skoog’s basal medium supplemented with 2% sucrose and 0.4% gellan gum powder, a gelling agent. These bottles were incubated in a specially designed plant growth chamber equipped with eight different LED lights consisting of single or combinations of four different light spectra emitting blue (450 nm), green (525 nm), red (660 nm), and far-red (730 nm) light. After three months of incubation, morphological changes in embryogenic calli were recorded, and LC-MS/MS analysis of cultures was carried out for peimisine, sipeimine, peiminine, and peimine. The highest number of somatic embryos and the maximum fresh weight was recorded in calli incubated under red (9R), infrared (9IR), and a combination of red+blue+infrared (3R3B3IR), respectively, in decreasing order. The highest contents of peimisine, peiminine, and peimine were recorded under red (9R) and infrared (9IR) lights, respectively. Eight LED lights had significant effects on the morphogenesis of embryogenic calli of F. cirrhosa D. Don and contents of isosteroidal alkaloids.


2017 ◽  
Vol 50 (6) ◽  
pp. 921-936 ◽  
Author(s):  
WJ Huang ◽  
Y Yang ◽  
M Ronnier Luo

This paper describes an experiment to investigate discomfort glare caused by white light-emitting diode (LED) lights having different spectral power distributions. It included two groups: a ‘Metamerism’ group and a ‘correlated colour temperatures (CCT)’ group. In the former group, it was found that white lights at 7000 K constructed from different blue LEDs and the same red and green LEDs gave about the same glare perception. In the latter group, there was a significant difference in glare perception between white lights having different CCTs. Finally, glare models, including unified glare rating (UGR) and the newly derived QUGRspd, and mUGRspd models, were tested using the data from the experiment. All of them gave quite accurate predictions of the data.


2019 ◽  
Vol 8 (3) ◽  
pp. 1977-1981

Visible Light Communications (VLC) system is called Light Fidelity One such example, analog data is passed to a LED (Light-Emitting Diode) bulb (using signal processing technology), after that it transmits data (which is implanted in its beam) to the detector or photodiode. The small commutes in the brisk feeble of LED bulbs is modulated by the receiver circuit into electrical signal. The signal is then reversed into a duplex data runnel that could be recognized in the same way that of audio, video and web programs that run on devices that has internet enabled. Wi-Fi is vulnerable to breaches involving various security issues such as Rouge Access Points, Denial of Service, Wireless Trespasser, Data Interruption, End point attacks etc. To overcome such restrictions, this paper proposes application level substructure for data communication using Li-Fi (Light Fidelity) Technology. By using LED lights as a transmission medium, the indoor wireless communication is achieved in much faster rate than the one WiFi (Wireless Fidelity) can provide.


Author(s):  
Kil-Nam Kim ◽  
Hye-Sung Song ◽  
Ryong-Jin Choe ◽  
Zhi-Juan Huang ◽  
Qiu-Ying Huang ◽  
...  

Recently, light traps using light-emitting diode (LED) lights have been applied to monitor or control insect pests. The oriental armyworm, Mythimna separata Walker, is an important insect pest that has caused damage to several cereal crops, including corn, wheat and rice. The present study aims to seek out a sensitive wavelength causing high phototactic response in M. separata. The study evaluated the phototactic responses of M. separata moths to several LED lights of different wavelengths and luminance intensities under laboratory condition. Results showed that green (520 nm) LED light resulted in significant phototactic response of M. separata moths compared to LED lights of other wavelengths. Additionally, the highest attraction rate of the moths to green LED light appeared in luminance intensity group of 200 lux compared to the other intensities groups. Experiments under optimum conditions based on the above experiments revealed that the green LED light exhibited the strongest attraction rate (64.44%) among all experimental groups. An experiment performed in a net cage also showed that green LED light resulted in the highest phototactic response of M. separata moths, 1.7 times more than a commercial black light used as control. These findings clearly demonstrate that M. separata moths have a high sensitivity to the green LED light. Therefore, a light trap equipped with green LED light could be useful for monitoring and controlling M. separata moths.


2018 ◽  
Vol 4 (2) ◽  
pp. 52
Author(s):  
Anwar Mujadin ◽  
Suci Rahmatia

<p><em>Abstrak – </em><strong>Joule-Thief adalah salah satu rangkaian listrik  untuk konservasi  energi dengan menggunakan teknis medan elektromagnetik arus transien pada sebuah coil. Sirkuit ini dioperasikan menggunakan  sebuah MOSFET sebagai saklar untuk meregenerasi  tegangan dan arus listrik dalam satuan waktu kemudian disalurkan pada beban light emitting diode (LED). Dari hasil eksperimental,  sel volta berbasis air laut dengan tegangan jepit 0.8V mampu mengaktifkan lampu LED dengan konfigurasi rangkaian serial. Hasilnya menunjukkan bahwa power transfer maksimum dari sirkuit Joule-thief sangat efektif sebagai pemasok daya beban 60 LED.</strong></p><p><strong> </strong></p><p><strong><em>Kata Kunci </em></strong><em>- <strong> </strong>Joule Thief, Boost Converter Daya LED, Sel Volta Berbasis Air Laut<strong></strong></em></p><p><em> </em></p><p><em>Abstract –</em> <strong>Joule-Thief is one of the electric circuits for conservation energy by using electromagnetic field trancient current in a coil. This circuit is operated using a MOSFET as a switch to regenerating the voltage and current flowing along the coil. The electric current accumulates in the coil then in unity of time will be supplied to the light emitting diode (LED) load. From experimental results, water sea voltaic cells based with 0.8V clamp voltage are able to activate LED lights in serial circuit configurations. The results shown that power transfer joule-thief circuit is very effective capable of supplying LED up to 60 LEDs.</strong></p><p><strong> </strong></p><p><strong><em>Keywords </em></strong><em>- Joule Thief, </em><em>Boost Converter Power LED</em><em>, </em><em>Sea-Based Voltaic Cells</em><em></em></p>


2019 ◽  
Vol 7 ◽  
pp. 44-51
Author(s):  
Indu Duwal

With the increment in the gap between the demand and supply of energy, various alternatives for decreasing the demand of energy are major concern for the various stakeholders. In order to decrease the energy demand by lighting various energy efficient lights are available in market. However it is difficult to convince the residents to change conventional artificial light sources, since lighting system is an essential part in the building which ensures the comfort, productivity and safety of the occupants in the buildings. Among the various Light Emitting Diode (LED) lights are termed as one of the most efficient energy saving lights which aims to save almost more than 50 % of energy demand than that of CFL lights with same power of illumination. Hence, this study is carried out to calculate energy saving percentage after replacing the conventional lights like florescent, mercury lights and other forms of conventional lights by LED lights. Additionally the study also was focused to evaluate feasibility of the replacement of lights by LED lights in terms of energy saving and return of investment. The research was carried out in building used by Agrotechnology and Food Science (AFSG) science group of Wageningen University in the Netherlands. The results from the study showed that almost 50% of the energy can be saved after replacement by LED lights with around 5 years for return of investment. However, factors like 1) Number of operating hours, 2) Price of lights, 3) Energy cost, 4) Labour cost for replacement and adjustment and 5) Number of replacement and adjustment per hour are the influential features that effects in the performance of various types of lights


2020 ◽  
Vol 10 (8) ◽  
pp. 2655 ◽  
Author(s):  
Yi Miao ◽  
Yuanyang Zhu ◽  
Wenzhu Zhao ◽  
Changshuai Jiao ◽  
Hongwei Mo ◽  
...  

A novel method was proposed for the determination of vitamin C (VC) using an infrared camera combined with the iodine-turbidimetric method. Based on the redox between VC and iodine, the residual iodine was measured using the turbidimetric method with an infrared camera to obtain VC content. The light emitted by the infrared light-emitting diode (LED) was absorbed and scattered when it penetrated the residual iodine suspension. The transmitted light was captured by the infrared camera to form a digital image and the responding color components and grayscale values were obtained. The obtained color components and log-grayscale were fitted to the VC concentration, and the fitted relation expressions were used to measure the unknown VC solution. A VC measuring device equipped with an infrared camera and processing software was designed to obtain the color components corresponding to the images of the iodine suspensions. Compared with the spectrophotometry, the method based on the color component of brightness had a higher accuracy for measuring the VC standard solution. For VC measurements in tomatoes, nectarines, and VC tablets, our proposed method was highly consistent with spectrophotometry. Therefore, this method could potentially be implemented in the determination of VC in fruits and tablets, or other foods.


2015 ◽  
Vol 21 (2) ◽  
pp. 61-78 ◽  
Author(s):  
P. Wesley Schultz ◽  
Julie Colehour ◽  
Jill Vohr ◽  
Lara Bonn ◽  
Ashlee Bullock ◽  
...  

Recent technological advancements in lighting have produced light bulbs that are dramatically more efficient than the traditional incandescent bulb. Yet despite these benefits, the marketplace has been slow to adopt these newer and more efficient bulbs. To help facilitate this change, the Environmental Protection Agency’s ENERGY STAR program conducted a series of pilot behavior change campaigns to encourage residents to purchase and install ENERGY STAR-certified light-emitting diode (LED) lights. Campaigns were conducted with Duke Energy and Efficiency Vermont aimed at encouraging residents to purchase and install certified LED lighting in their homes. The campaigns used community-based social marketing (CBSM) as the platform for developing the overarching strategy and were developed to highlight the benefits of certified LED lights and to decrease the barriers. In Vermont, a school-based fund-raiser was used to promote certified LED bulbs, and in North Carolina, special in-store lighting events were held at Home Depot and Costco retail locations. Both programs were implemented using a control group, and quantitative outcomes were tracked. Reported results highlight the increase in sales of certified LEDs associated with the campaigns. Survey data are also reported from participants in the two campaigns, along with tests for spillover of the newly adopted behavior into other lighting purchases and behaviors. The lessons learned from these two CBSM pilots are synthesized into turnkey materials that can be adopted by other organizations looking to promote the adoption of LED lights.


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