scholarly journals Specificities of Scanning Electron Microscopy and Histological Methods in Assessing Cell-Engineered Construct Effectiveness for the Recovery of Hyaline Cartilage

2021 ◽  
Vol 4 (4) ◽  
pp. 77
Author(s):  
Mikhail S. Bozhokin ◽  
Svetlana A. Bozhkova ◽  
Aleksandr A. Rubel ◽  
Julia V. Sopova ◽  
Yulia A. Nashchekina ◽  
...  
Keyword(s):  
Electron Microscopy ◽  
Scanning Electron Microscopy ◽  
Hyaline Cartilage ◽  
Articular Surface ◽  
Experimental Methods ◽  
Urgent Problem ◽  
Biodegradable Scaffold ◽  
The World ◽  
A Cell ◽  
Scanning Electron

Damage to the hyaline layer of the articular surface is an urgent problem for millions of people around the world. At present, a large number of experimental methods are being developed to address this problem, including the transplantation of a cell-engineered construct (CEC) composed of a biodegradable scaffold with a premixed cell culture into the damaged area of the articular surface. However, current methods for analyzing the effectiveness of such CECs have significant limitations. This study aimed to compare the SEM technique, classical histology, and cryosectioning for the analysis of CECs transplanted to hyaline cartilage.

scholarly journals Capture and scanning electron microscopy of individual snow crystals

1994 ◽  
Vol 40 (134) ◽  
pp. 195-197
Author(s):  
E. W. Wolff ◽  
A. P. Reid
Keyword(s):  
Electron Microscopy ◽  
Scanning Electron Microscopy ◽  
Electron Microscope ◽  
Scanning Electron Microscope ◽  
Liquid Nitrogen Temperature ◽  
Experimental Methods ◽  
Snow Crystal ◽  
Snow Crystals ◽  
First Time ◽  
Scanning Electron

AbstractA snow crystal has been successfully collected on to a scanning electron microscope (SEM) stub in central Greenland. It was preserved at liquid-nitrogen temperature for 5 months, prior to examination in the SEM. This is believed to be the first time a snow crystal has been observed directly in the SEM and offers some new experimental methods for understanding crystals and their chemistry.

scholarly journals Analysis of the type material of Achnanthes minutissima var. macrocephala (Bacillariophyta) and description of two new small capitate Achnanthidium species from Europe and the Himalaya

2019 ◽  
Vol 152 (2) ◽  
pp. 340-350
Author(s):  
Carlos E. Wetzel ◽  
Ingrid Jüttner ◽  
Smriti Gurung ◽  
Luc Ector
Keyword(s):  
Electron Microscopy ◽  
Scanning Electron Microscopy ◽  
Benthic Diatom ◽  
Original Material ◽  
Diatom Species ◽  
The World ◽  
Freshwater Habitats ◽  
The Himalaya ◽  
Scanning Electron ◽  
Achnanthes Minutissima

Background and aims – Two unknown benthic diatom species belonging to the genus Achnanthidium Kütz., and found in French and Nepalese freshwater habitats, were investigated. Both species are here described as new and compared with the original material of Achnanthes minutissima var. macrocephala Hust. [≡ Achnanthidium macrocephalum (Hust.) Round & Bukht.] from Indonesia. Methods – The morphology of three small and capitate Achnanthidium species was investigated using light microscopy (LM) and scanning electron microscopy (SEM). Key results – Achnanthidium coxianum sp. nov. (from the Himalaya) belongs to the group of species with hooked terminal raphe endings, while Achnanthidium peetersianum sp. nov. (from France) has variable terminal raphe endings which are usually slightly bent. Achnanthidium macrocephalum is a much rarer species and illustrations concerning its identity in the literature do not conform to the type studied here. Conclusions – The three species are similar in LM but clearly distinct in SEM. The shape of the areolae and terminal raphe endings separate the species. Achnanthidium macrocephalum has often been misidentified in studies from many areas of the world. It is similar to the new species in valve outline and in its small dimensions.

The intervertebral joint of the longnose gar, Lepisosteus osseus

1974 ◽  
Vol 52 (7) ◽  
pp. 803-804 ◽  
Author(s):  
H. U. Cameron
Keyword(s):  
Electron Microscopy ◽  
Scanning Electron Microscopy ◽  
Articular Cartilage ◽  
Hyaline Cartilage ◽  
Superficial Layer ◽  
Joint Cavity ◽  
Staining Techniques ◽  
Longnose Gar ◽  
Histological Staining ◽  
Scanning Electron

The intervertebral joint of the longnose gar has been examined by differential histological staining techniques and by scanning electron microscopy. The joint was found to be transitional, being neither a true diarthrosis nor a synchondrosis. The articular cartilage was found to consist of two layers, a superficial layer of fibrocartilage and a deeper layer of hyaline cartilage. The joint cavity was partially filled with fibrocartilaginous adhesions, the number of which varied from joint to joint.The degree of movement in each joint was minimal, in keeping with the heavily armored exoskeleton.

Four new Luticola taxa (Bacillariophyta) from Maritime Antarctica 

Phytotaxa ◽  
2014 ◽  
Vol 170 (3) ◽  
pp. 155 ◽  
Author(s):  
RALITSA ZIDAROVA ◽  
ZLATKO LEVKOV ◽  
BART VAN DE VIJVER
Keyword(s):  
Electron Microscopy ◽  
Scanning Electron Microscopy ◽  
New Taxa ◽  
Similar Species ◽  
The South ◽  
Maritime Antarctica ◽  
World Data ◽  
The World ◽  
Scanning Electron

The paper describes four new Luticola taxa from the South Shetland Archipelago, Antarctica:  Luticola neglecta sp. nov., L. bogaertsiana sp. nov., L. contii sp. nov. and L. olegsakharovii sp. nov. All new taxa are studied using both light and scanning electron microscopy and compared to other morphologically similar species from (sub-)Antarctica and elsewhere in the world. Data on their ecology and biogeography are added. 

Comparison of two new species of astogloia Bacillariophyceae) with other small members of section Ellipticae

Phytotaxa ◽  
2013 ◽  
Vol 126 (1) ◽  
pp. 1 ◽  
Author(s):  
CHIARA PENNESI ◽  
MICHEL POULIN ◽  
FRIEDEL HINZ ◽  
TIZIANA ROMAGNOLI ◽  
MARIO DE STEFANO ◽  
...  
Keyword(s):  
Electron Microscopy ◽  
Scanning Electron Microscopy ◽  
New Species ◽  
Geographical Distribution ◽  
New Taxa ◽  
Related Species ◽  
The World ◽  
Two New Species ◽  
Scanning Electron

In this study, seven Mastogloia species belonging of the section Ellipticae are morphologically described through scanning electron microscopy, including two new taxa M. matthaei and M. stellae. They were collected as epiphytes on seagrasses from several tropical (Indonesia), subtropical (Egypt, Greece, Republic of Malta, Turkey) and temperate (Slovenia, Italy) regions of the world. All these species show typical characters of the Hustedt’s section Ellipticae: elliptical valve outline, a flat valve surface and rounded apices. Moreover, this study provides novel information on the frustule ultrastructure and gives update of their current geographical distribution. Some nomenclatural inconsistencies have been resolved with the typification of M. ovulum and the related species, M. emarginata, from the original Miang Besar material in the Hustedt collection.

scholarly journals Capture and scanning electron microscopy of individual snow crystals

1994 ◽  
Vol 40 (134) ◽  
pp. 195-197 ◽  
Author(s):  
E. W. Wolff ◽  
A. P. Reid
Keyword(s):  
Electron Microscopy ◽  
Scanning Electron Microscopy ◽  
Electron Microscope ◽  
Scanning Electron Microscope ◽  
Liquid Nitrogen Temperature ◽  
Experimental Methods ◽  
Snow Crystal ◽  
Snow Crystals ◽  
First Time ◽  
Scanning Electron

AbstractA snow crystal has been successfully collected on to a scanning electron microscope (SEM) stub in central Greenland. It was preserved at liquid-nitrogen temperature for 5 months, prior to examination in the SEM. This is believed to be the first time a snow crystal has been observed directly in the SEM and offers some new experimental methods for understanding crystals and their chemistry.

Effect of Cooling Stimulus on Collection Efficiency of Calf Chondrocytes Cultivated on Metal Surface

2017 ◽  
Vol 11 (6) ◽  
pp. 925-931 ◽  
Author(s):  
Yuta Kurashina ◽  
◽  
Shogo Miyata ◽  
Jun Komotori
Keyword(s):  
Electron Microscopy ◽  
Scanning Electron Microscopy ◽  
Cell Culture ◽  
Cell Proliferation ◽  
Collection Efficiency ◽  
Trypsin Treatment ◽  
Culture Substrate ◽  
A Cell ◽  
Number Of Cells ◽  
Scanning Electron

A cell culture module capable of cooling stimulus to collect cells efficiently on a metal culture substrate was developed. We evaluated the cell collection ratio and morphology of the collected cells. Following a cooling stimulus (0°C) for 20 min, the number of collected cells was increased by 50% compared to that collected after trypsin treatment without pipetting from the metal culture substrate. Following the cooling stimulus, cells were observed by fluorescence microscopy and scanning electron microscopy; the cell filopodia were shrunken compared to non-cooling-stimulated cells. Furthermore, the combination of collagenase and cooling stimulation resulted in the collection of a comparable number of cells as that obtained using only trypsin. Thus, cell proliferation was improved compared to that following trypsin treatment. Therefore, this method can be applied for culturing cells that are susceptible to trypsin damage.

scholarly journals A high-yield technique for preparing cells fixed in suspension for scanning electron microscopy.

1975 ◽  
Vol 67 (2) ◽  
pp. 476-480 ◽  
Author(s):  
S K Sanders ◽  
E L Alexander ◽  
R C Braylan
Keyword(s):  
Electron Microscopy ◽  
Scanning Electron Microscopy ◽  
Electron Microscope ◽  
Scanning Electron Microscope ◽  
Quantitative Comparison ◽  
High Yield ◽  
Live Cells ◽  
A Cell ◽  
Number Of Cells ◽  
Scanning Electron

Human leukocytes fixed in suspension were allowed to settle onto poly-L-lysine-coated glass coverslips and prepared for observation with the scanning electron microscope (SEM). The coverslips were dehydrated in ethanol, critical point dried with CO2, and coated with gold-palladium. With the aid of a locator grid, several fields were photographed with light microscopy after the cells had settled onto the poly-L-lysine-coated coverslips and again after completion of the processing before SEM observation. Quantitative comparison of the number of cells present after settling with the number retained for final viewing with the SEM revealed a cell yield approaching 100%. This simple, reproducible, high-yield technique for processing cells fixed in suspension for SEM prevents changes in surface architecture induced by collecting live cells onto various substrates before fixation and also avoids potentially selective cell losses. Such a technique should allow quantitative correlations between SEM and other morphological and functional parameters.

A new species of the diatom genus Campylodiscus (Bacillariophyta) from Dongtan, Chongming Island, China

Phytotaxa ◽  
2013 ◽  
Vol 115 (2) ◽  
pp. 49
Author(s):  
Patrick Kociolek ◽  
Bo Wu ◽  
Qi Liu ◽  
Quanxi Wang
Keyword(s):  
Electron Microscopy ◽  
Scanning Electron Microscopy ◽  
New Species ◽  
Small Species ◽  
Chongming Island ◽  
The World ◽  
Diatom Genus ◽  
A New Species ◽  
Scanning Electron

A new species of genus Campylodiscus is described, Campylodiscus sinensis sp. nov., from Dongtan, Chongming Island, Shanghai, China. It is a small species compared with other species in genus Campylodiscus, with two characteristic kidney-shaped bumps externally and pyramidal verrucae. Light and scanning electron microscopy characteristics of this taxon support its systematic placement in the genus Campylodiscus. This new species is compared with other small species in genus Campylodiscus from around the world. 

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