Silver Nanoparticles Stable to Oxidation and Silver Ion Release Show Size-Dependent Toxicity In Vivo

Silver nanoparticles (AgNPs) are widely used in commerce, however, the effect of their physicochemical properties on toxicity remains debatable because of the confounding presence of Ag+ ions. Thus, we designed a series of AgNPs that are stable to surface oxidation and Ag+ ion release. AgNPs were coated with a hybrid lipid membrane comprised of L-phosphatidylcholine (PC), sodium oleate (SOA), and a stoichiometric amount of hexanethiol (HT) to produce oxidant-resistant AgNPs, Ag–SOA–PC–HT. The stability of 7-month aged, 20–100 nm Ag–SOA–PC–HT NPs were assessed using UV–Vis, dynamic light scattering (DLS), and inductively coupled plasma mass spectrometry (ICP-MS), while the toxicity of the nanomaterials was assessed using a well-established, 5-day embryonic zebrafish assay at concentrations ranging from 0–12 mg/L. There was no change in the size of the AgNPs from freshly made samples or 7-month aged samples and minimal Ag+ ion release (<0.2%) in fishwater (FW) up to seven days. Toxicity studies revealed AgNP size- and concentration-dependent effects. Increased mortality and sublethal morphological abnormalities were observed at higher concentrations with smaller nanoparticle sizes. This study, for the first time, determined the effect of AgNP size on toxicity in the absence of Ag+ ions as a confounding variable.

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Results of an interlaboratory method performance study for the size determination and quantification of silver nanoparticles in chicken meat by single-particle inductively coupled plasma mass spectrometry (sp-ICP-MS)

Analytical and Bioanalytical Chemistry ◽

10.1007/s00216-017-0427-2 ◽

2017 ◽

Vol 409 (20) ◽

pp. 4839-4848 ◽

Cited By ~ 18

Author(s):

Stefan Weigel ◽

Ruud Peters ◽

Katrin Loeschner ◽

Ringo Grombe ◽

Thomas P. J. Linsinger

Keyword(s):

Mass Spectrometry ◽

Silver Nanoparticles ◽

Single Particle ◽

Inductively Coupled Plasma ◽

Size Determination ◽

Performance Study ◽

Method Performance ◽

Inductively Coupled ◽

Icp Ms ◽

Plasma Mass Spectrometry

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Metal Ion Release and Cytotoxicity of Titanium Orthodontic Miniscrews

Key Engineering Materials ◽

10.4028/www.scientific.net/kem.730.141 ◽

2017 ◽

Vol 730 ◽

pp. 141-147

Author(s):

Katha Kosayadiloka ◽

Nathaphon Tangjit ◽

Suwannee Luppanapornlarp ◽

Peerapong Santiwong

Keyword(s):

Inductively Coupled Plasma ◽

Metal Ion ◽

The Self ◽

Cytotoxicity Test ◽

Ion Release ◽

Element Analysis ◽

Inductively Coupled ◽

Icp Ms ◽

Metal Ion Release ◽

Plasma Mass Spectrometry

The aim of this study was to investigate the metal ion release and cytotoxicity of MU orthodontic miniscrews as well as two other brands of orthodontic miniscrews over time. Twenty-four orthodontic miniscrews were tested, divided into three groups of eight. Each sample extraction was performed following the ISO 10993-12:2012 method. Solutions were collected after 1, 7, and 30 days (T1, T2, and T3). The supernatants extracted from these three groups were added and exposed to mouse L929 fibroblastic cell line using an MTT cytotoxicity test. They were also tested for ion release by inductively coupled plasma-mass spectrometry (ICP-MS). Element analysis by energy-dispersive X-ray spectroscopy (EDS) was used to analyze the surfaces of the miniscrews. The quantification of three elements, namely, titanium (Ti), aluminum (Al), and vanadium (V) were assessed. The results indicated that there were no statistical differences between the self-made orthodontic miniscrews and those from two commercial groups (p<0.05). Throughout the testing period, the quantity of ions increased from T1 to T3. After 24 h, vanadium was the first to appear on the surface in small quantities in other two commercial groups. The self-made orthodontic miniscrews exhibited no toxic effects on living cells.

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A preliminary report on bioavailability of Ayurvedic Gold Bhasma with four different accompanying media [anupana]

World Journal of Advanced Research and Reviews ◽

10.30574/wjarr.2021.12.3.0540 ◽

2021 ◽

Vol 12 (3) ◽

pp. 033-044

Author(s):

Trupti Patil ◽

Asmita Wele ◽

Sangram Patil

Keyword(s):

Inductively Coupled Plasma ◽

Black Pepper ◽

Healthy Human ◽

Blood Samples ◽

Inductively Coupled ◽

Icp Ms ◽

Plasma Mass Spectrometry ◽

Comparative Bioavailability ◽

Human Participants

Background: Gold bhasma [Swarnabhasma] is a ancient Ayurvedic medicine used for rejuvenation and longevity. This is a preliminary attempt to study the bioavailability of this medicine. Objectives: It was aimed to estimate comparative bioavailability of gold bhasma up to five hours after oral dose with four different anupana. Materials and methods: In this in vivo study, 30 healthy human participants were allocated randomly into five groups having six individuals each. Gold bhasma in 30 mg dose was administered orally with four different anupana viz honey [2.5 gm], black pepper-ghee combination [250 mg and 2.5 gm respectively], lactose [250 mg], glucose [250 mg], and plain to participants in each group. Blood samples were collected at 0, 1, 3 and 5 hours after dose. Gold levels in blood were assessed by inductively coupled plasma mass spectrometry [ICP-MS]. Results: Gold levels in all blood samples were in traces. Average Cmax was 0.002333 microgram of gold per liter and Tmax was at 3 hours for honey anupana, showing maximum Cmax among all groups. Conclusions: Preliminary results indicate that bioavailability of gold from gold bhasma may be less than 0.01 % upto first five hours. Gold bhasma mixed with honey resulted in attainment of maximum Cmax. It is evident that accompanying media [anupana] play an important role in absorption of gold bhasma.

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Stability of Bioactivated Co-Cr Alloys in Biological Environment

Key Engineering Materials ◽

10.4028/www.scientific.net/kem.361-363.737 ◽

2007 ◽

Vol 361-363 ◽

pp. 737-740 ◽

Cited By ~ 2

Author(s):

M.M. Sovar ◽

C. Ducu ◽

D. Iordachescu ◽

Ioana Demetrescu

Keyword(s):

Inductively Coupled Plasma ◽

Open Circuit ◽

X Ray ◽

Inductively Coupled ◽

Cyclic Voltametry ◽

Icp Ms ◽

Plasma Mass Spectrometry ◽

Biological Environment ◽

The Stability ◽

Electronic Microscope

The present work is focused on the stability of bioactivated CoCr alloy in biological environment (buffered saline solution (PBS), lactic acid, citric acid). The chemical and electrochemical deposition was characterized by electrochemical methods (open circuit potential, cyclic voltametry), scanning electronic microscope (SEM), x-ray diffractometer (XRD), inductively coupled plasma/mass spectrometry (ICP/MS) and citotoxicity test. The results prove a good electrochemical stability in all cases.

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DETECÇÃO E QUANTIFICAÇÃO DE NANOPARTÍCULAS DE PRATA POR SPICP-MS

Química Nova ◽

10.21577/0100-4042.20170735 ◽

2021 ◽

Author(s):

Fabio Bazilio ◽

Cristiane Silva ◽

Lísia Santos ◽

Santos Vicentini ◽

Silvana Jacob ◽

...

Keyword(s):

Mass Spectrometry ◽

Silver Nanoparticles ◽

Satisfactory Result ◽

Inductively Coupled Plasma ◽

Metallic Nanoparticles ◽

Particle Concentration ◽

Inductively Coupled ◽

Icp Ms ◽

Plasma Mass Spectrometry ◽

Detection And Quantification

DETECTION AND QUANTIFICATION OF SILVER NANOPARTICLES BY spICP-MS. The growing interest in nanotechnology has led to an increase in the production and application of nanoparticles worldwide. Due to the unique functional properties of nanoparticles, these materials are being used by many industries, including the agricultural and food sectors. Among the commercially available nanomaterials, it is possible to highlight those produced with silver nanoparticles. One of the most promising techniques for the analysis of metallic nanoparticles is the Inductively coupled plasma mass spectrometry (ICP-MS) performed in single particle mode (spICP-MS). However, the use of the technique in the detection and measurement of nanoparticles requires that the equipment be operated differently than when used to analyze dissolved metal solutions. Thus, this article presents the use of the spICP-MS technique for the measurement and quantification of nanoparticles, as well as their validation. The method proved to be adequate for the purpose, presenting a satisfactory result for the selectivity test and recovery of 83.7 (40 nm) and 77.6% (80 nm). The detection limits, determined for the most frequent size, dissolved silver concentration and particle concentration, were 17.5 nm, 0.736 ng mL-1,146 particles mL-1, respectively. Thus, the results obtained indicated the possibility of using the technique to determine the size and concentration of silver nanoparticles.

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Biocompatibility of Near-IR Sensitive Au-Based Nanoparticles as the Potential Drug Delivery Carriers

Key Engineering Materials ◽

10.4028/www.scientific.net/kem.334-335.1177 ◽

2007 ◽

Vol 334-335 ◽

pp. 1177-1180 ◽

Cited By ~ 1

Author(s):

Bin Zhang ◽

Xiao Li Huang ◽

Lei Ren ◽

Qi Qing Zhang ◽

Mei Chee Tan ◽

...

Keyword(s):

Inductively Coupled Plasma ◽

Near Infrared ◽

Nih3t3 Cells ◽

Inductively Coupled ◽

Near Ir ◽

Icp Ms ◽

Plasma Mass Spectrometry ◽

Good Biocompatibility

We successfully synthesized near infrared (NIR) sensitive Au(shell)-Au2S(core) nanoparticles, where Au2S dielectric core was encapsulated by a thin gold shell. The cytotoxicity in vitro and biodistribution in vivo of Au-Au2S nanoparticles was studied by using NIH3T3 cells and KM mice, respectively. The quantitative analysis of Au in each tissue of mice was done by using the Inductively Coupled Plasma Mass Spectrometry (ICP-MS). Au-Au2S nanoparticles (< 300 μg/ml) showed good biocompatibility. Au-Au2S nanoparticles were preferentially taken up by the liver and spleen, and ultimately eliminated mostly in the feces.

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Analysis of Silver Nanoparticles Using Single-Particle Inductively Coupled Plasma – Mass Spectrometry (ICP-MS): Parameters Affecting the Quality of Results

Analytical Letters ◽

10.1080/00032719.2018.1459657 ◽

2018 ◽

Vol 52 (2) ◽

pp. 288-307 ◽

Cited By ~ 7

Author(s):

Martin Loula ◽

Antonín Kaňa ◽

Richard Koplík ◽

Jaroslav Hanuš ◽

Magda Vosmanská ◽

...

Keyword(s):

Mass Spectrometry ◽

Silver Nanoparticles ◽

Single Particle ◽

Inductively Coupled Plasma ◽

Quality Of Results ◽

Inductively Coupled ◽

Icp Ms ◽

Plasma Mass Spectrometry

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Development and Evaluation of a System for the Semi-Quantitative Determination of the Physical Properties of Skin After Exposure to Silver Nanoparticles

Nanoscale Research Letters ◽

10.1186/s11671-020-03421-x ◽

2020 ◽

Vol 15 (1) ◽

Author(s):

Hong Tao ◽

Kazuya Nagano ◽

Ikkei Tasaki ◽

Tian-qi Zhang ◽

Takuya Ishizaka ◽

...

Keyword(s):

Silver Nanoparticles ◽

Physical Properties ◽

Inductively Coupled Plasma ◽

Complete Separation ◽

Inductively Coupled ◽

Ms Analysis ◽

Icp Ms ◽

Particle Form ◽

Analytical System

Abstract In order to ensure the safe usage of silver nanoparticles (nAgs) in cosmetics, it is necessary to reveal the physical properties of nAgs inside the skin, as these properties may change during the process of percutaneous absorption. In this study, we aimed to establish an analytical system based on single particle inductively coupled plasma mass spectrometry (sp-ICP-MS) to determine the physical properties of nAgs in the skin. First, we optimized a pretreatment method for solubilizing the skin samples and then showed that most of the nAgs were recovered by sodium hydroxide treatment while remaining in particle form. For separating the skin into the epidermis and dermis, we screened several conditions of microwave irradiation. The sp-ICP-MS analysis indicated that the application of 200 W for 30 s was optimal, as this condition ensured complete separation of skin layers without changing the physical properties of the majority of nAgs. Finally, we evaluated the in vivo application by analyzing the quantity as well as the physical properties of Ag in the epidermis, dermis, and peripheral blood of mice after exposing the skin to nAgs or Ag+. Subsequent sp-ICP-MS analysis indicated that nAgs could be absorbed and distributed into the deeper layers in the ionized form, whereas Ag+ was absorbed and distributed without a change in physical properties. This study indicates that in order to obtain a comprehensive understanding of the response of skin following exposure to nAgs, it is essential to consider the distribution and particle size of not only nAgs but also Ag+ released from nAgs into the skin.

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