The Relationships between Total Protein Intake, Protein Sources, Physical Activity, and Lean Mass in a Representative Sample of the US Adults

Background: Although dietary protein and physical activity play essential roles in developing and preserving lean mass, studies exploring these relationships are inconsistent, and large-scale studies on sources of protein and lean mass are lacking. Accordingly, the present study examined the relationship between total protein intake, protein sources, physical activity, and lean mass in a representative sample of US adults. Methods: This cross-sectional study analyzed data from 2011–2016 US National Health and Nutrition Examination Survey and corresponding Food Patterns Equivalents Database (n = 7547). Multiple linear regression models were performed to examine the sex-specific associations between total protein intake, protein sources (Dairy, Total Protein Foods, Seafood, and Plant Proteins), physical activity, and lean mass adjusting for demographics, weight status, and total daily energy intake. Results: Total protein intake was inversely related to lean mass in females only (Lean mass index: β= −0.84, 95%CI: −1.06–−0.62; Appendicular lean mass index: β= −0.35, 95%CI: −0.48–−0.22). However, protein sources and physical activity was positively associated with lean mass in males and/or females (p < 0.05). Conclusion. Study results suggest that consuming more protein daily had a detrimental influence on lean mass in females whereas eating high-quality sources of proteins and being physically active are important for lean mass for men and women. However, the importance of specific protein sources appears to differ by sex and warrants further investigation.

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The Relationship Between Healthy Eating Motivation and Protein Intake in Community-Dwelling Older Adults With Varying Functional Status

Nutrients ◽

10.3390/nu12030662 ◽

2020 ◽

Vol 12 (3) ◽

pp. 662

Author(s):

Hanna M. Rempe ◽

Gudrun Sproesser ◽

Anne Hannink ◽

Thomas Skurk ◽

Beate Brandl ◽

...

Keyword(s):

Older Adults ◽

Functional Status ◽

Healthy Eating ◽

Total Protein ◽

Protein Intake ◽

Community Dwelling ◽

Cross Sectional ◽

Protein Sources ◽

Total Protein Intake ◽

The Relationship

In older adults, the relationship between healthy eating motivation (HEM) and protein intake as key component of a healthy diet is poorly understood. Therefore, we investigate the association of HEM with (1) total protein intake and (2) intake of different protein sources in older adults with varying functional status. In this cross-sectional study including 250 adults (≥70 years), we assessed HEM with “The Eating Motivation Survey” and protein intake by 7-day food records. In addition, gender, age, Body Mass Index (BMI), energy intake and functional status were considered. Regression analyses revealed that HEM was neither related to total (β = −0.02; p = 0.723) nor to relative protein intake (β = 0.04; p = 0.370). Notwithstanding this, participants with stronger HEM showed lower intake of protein from meat and meat products (β = −0.14; p = 0.018), higher intake of overall plant-based protein (β = 0.11; p = 0.032), protein from fruit and vegetables (β = 0.20; p = 0.002) and from pulses, nuts an seeds (β = 0.16; p = 0.016). Our findings suggest HEM as a valuable indicator for intake of distinct protein sources. However, since HEM is not related to total protein intake, the importance of sufficient protein consumption should be emphasized by promoting healthy eating, regardless of self-perceived HEM.

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Dietary proteins and protein sources and risk of death: the Kuopio Ischaemic Heart Disease Risk Factor Study

American Journal of Clinical Nutrition ◽

10.1093/ajcn/nqz025 ◽

2019 ◽

Vol 109 (5) ◽

pp. 1462-1471 ◽

Cited By ~ 26

Author(s):

Heli E K Virtanen ◽

Sari Voutilainen ◽

Timo T Koskinen ◽

Jaakko Mursu ◽

Petra Kokko ◽

...

Keyword(s):

Total Protein ◽

Protein Intake ◽

Disease Risk ◽

Plant Protein ◽

Disease Risk Factor ◽

Protein Sources ◽

Total Protein Intake ◽

Risk Factor Study ◽

Factor Study ◽

Heart Disease Risk

ABSTRACT Background Previous studies investigating protein intake in relation to mortality have provided conflicting results. Objective We investigated the associations of dietary protein and protein sources with risk of disease death in the prospective, population-based Kuopio Ischaemic Heart Disease Risk Factor Study. Methods The study population consisted of 2641 Finnish men, aged 42–60 y at baseline in 1984–1989. We estimated protein intakes with 4-d dietary records at baseline and collected data on disease deaths from the national Causes of Death Register. Cox proportional hazards regression models were used to estimate HRs and 95% CIs. Results During the average follow-up of 22.3 y, we observed 1225 deaths due to disease. Higher intakes of total protein and animal protein had borderline statistically significant associations with increased mortality risk: multivariable-adjusted HR (95% CI) in the highest compared with the lowest quartile for total protein intake = 1.17 (0.99, 1.39; P-trend across quartiles = 0.07) and for animal protein intake = 1.13 (0.95, 1.35; P-trend = 0.04). Higher animal-to-plant protein ratio (extreme-quartile HR = 1.23; 95% CI: 1.02, 1.49; P-trend = 0.01) and higher meat intake (extreme-quartile HR = 1.23; 95% CI: 1.04, 1.47; P-trend = 0.01) were associated with increased mortality. When evaluated based on disease history at baseline, the association of total protein with mortality appeared more evident among those with a history of type 2 diabetes, cardiovascular disease, or cancer (n = 1094) compared with those without disease history (n = 1547) (P-interaction = 0.05 or 0.07, depending on the model). Intakes of fish, eggs, dairy, or plant protein sources were not associated with mortality. Conclusions Higher ratio of animal to plant protein in diet and higher meat intake were associated with increased mortality risk. Higher total protein intake appeared to be associated with mortality mainly among those with a predisposing disease. This trial was registered at clinicaltrials.gov as NCT03221127.

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Association of Daily Physical Activity and Sedentary Behaviour with Protein Intake Patterns in Older Adults: A Multi-Study Analysis across Five Countries

Nutrients ◽

10.3390/nu13082574 ◽

2021 ◽

Vol 13 (8) ◽

pp. 2574

Author(s):

Ilianna Lourida ◽

Jolanda M. A. Boer ◽

Ruth Teh ◽

Ngaire Kerse ◽

Nuno Mendonça ◽

...

Keyword(s):

Physical Activity ◽

Older Adults ◽

Sedentary Behaviour ◽

Energy Intake ◽

Total Protein ◽

Protein Intake ◽

Community Dwelling ◽

Study Analysis ◽

Total Protein Intake ◽

Eating Occasions

Physical activity and protein intake are associated with ageing-related outcomes, including loss of muscle strength and functional decline, so may contribute to strategies to improve healthy ageing. We investigated the cross-sectional associations between physical activity or sedentary behaviour and protein intake patterns in community-dwelling older adults across five countries. Self-reported physical activity and dietary intake data were obtained from two cohort studies (Newcastle 85+ Study, UK; LiLACS, New Zealand Māori and Non-Māori) and three national food consumption surveys (DNFCS, The Netherlands; FINDIET, Finland; INRAN-SCAI, Italy). Associations between physical activity and total protein intake, number of eating occasions providing protein, number of meals with specified protein thresholds, and protein intake distribution over the day (calculated as a coefficient of variance) were assessed by regression and repeated measures ANOVA models adjusting for covariates. Greater physical activity was associated with higher total protein intake and more eating occasions containing protein, although associations were mostly explained by higher energy intake. Comparable associations were observed for sedentary behaviour in older adults in Italy. Evidence for older people with higher physical activity or less sedentary behaviour achieving more meals with specified protein levels was mixed across the five countries. A skewed protein distribution was observed, with most protein consumed at midday and evening meals without significant differences between physical activity or sedentary behaviour levels. Findings from this multi-study analysis indicate there is little evidence that total protein and protein intake patterns, irrespective of energy intake, differ by physical activity or sedentary behaviour levels in older adults.

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Association between dietary protein intake and the risk of depressive symptoms in adults

British Journal Of Nutrition ◽

10.1017/s0007114520000562 ◽

2020 ◽

Vol 123 (11) ◽

pp. 1290-1301 ◽

Cited By ~ 3

Author(s):

Yan Li ◽

Caixia Zhang ◽

Suyun Li ◽

Dongfeng Zhang

Keyword(s):

Depressive Symptoms ◽

Dietary Protein ◽

Total Protein ◽

Protein Intake ◽

Public Health Problem ◽

Response Analysis ◽

Dietary Protein Intake ◽

Milk Products ◽

Protein Sources ◽

Total Protein Intake

AbstractDepression is an important public health problem. The aim of the study is to explore the associations of total protein intake and protein sources with the risk of depressive symptoms. This cross-sectional study used data from the National Health and Nutrition Examination Survey for the years 2007–2014. Dietary protein intake was obtained from two 24-h dietary recall interviews. Depressive symptoms were assessed by a nine-item Patient Health Questionnaire. Logistic regression models and restricted cubic spline models were used to estimate the associations of total protein intake (g/kg per d) and protein sources with the risk of depressive symptoms. A total of 17 845 individuals aged 18 years and older were included in this study. Total protein intake was inversely associated with the risk of depressive symptoms. The full-adjusted OR of depressive symptoms was 0·34 (95 % CI 0·17, 0·68) for quartile (Q) 4 v. Q1 of total protein intake. For protein intake from milk and milk products, the association with depressive symptoms was significant both for Q2 v. Q1 (OR 0·61; 95 % CI 0·41, 0·93) and Q3 v. Q1 (OR 0·37; 95 % CI 0·24, 0·59) in the full-adjusted model. In the dose–response analysis, the shape of the associations of total protein intake and protein intake from milk and milk products with the risk of depressive symptoms was approximately L-shaped and U-shaped, respectively. The present study demonstrated that total protein intake and protein intake from milk and milk products might reduce the risk of depressive symptoms in US adults.

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Dietary Protein Sources and Muscle Mass over the Life Course: The Lifelines Cohort Study

Nutrients ◽

10.3390/nu10101471 ◽

2018 ◽

Vol 10 (10) ◽

pp. 1471 ◽

Cited By ~ 18

Author(s):

Nikita Alexandrov ◽

Coby Eelderink ◽

Cécile Singh-Povel ◽

Gerjan Navis ◽

Stephan Bakker ◽

...

Keyword(s):

Muscle Mass ◽

Dietary Protein ◽

Total Protein ◽

Protein Intake ◽

Animal Protein ◽

Creatinine Excretion ◽

Protein Sources ◽

Fish Meat ◽

Total Protein Intake ◽

Egg Protein

The influence of dietary protein intake on muscle mass in adults remains unclear. Our objective was to investigate the association between protein intake and muscle mass in 31,278 men and 45,355 women from the Lifelines Cohort. Protein intake was estimated by food frequency questionnaire and muscle mass was estimated from 24 h urinary creatinine excretion. The age range was 18–91 years and mean total protein intake was 1.0 ± 0.3 g/kg/day. Across increasing quartiles of total protein intake, animal protein intake, and fish/meat/egg protein intake, creatinine excretion significantly increased in both men (+4% for total and +6% for fish/meat/egg protein intake, p < 0.001) and women (+3% for total and +6% for fish/meat/egg protein intake, p < 0.001). The associations were not systematically stronger or weaker with increasing age, but associations were strongest for young men (26–45 years) and older women (>75 years). The association between total protein intake and muscle mass was dependent on physical activity in women (p interaction < 0.001). This study suggests that total protein intake, animal protein intake, and in particular fish/meat/egg protein intake may be important for building and preserving muscle mass. Dietary protein sources should be further studied for their potential to build and preserve muscle mass.

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Dietary Intake, Nutritional Adequacy, and Food Sources of Protein and Relationships with Personal and Family Factors in Spanish Children Aged One to < 10 Years: Findings of the EsNuPI Study

Nutrients ◽

10.3390/nu13041062 ◽

2021 ◽

Vol 13 (4) ◽

pp. 1062

Author(s):

Casandra Madrigal ◽

María José Soto-Méndez ◽

Ángela Hernández-Ruiz ◽

Teresa Valero ◽

Federico Lara Villoslada ◽

...

Keyword(s):

Dietary Protein ◽

Total Protein ◽

Dairy Products ◽

Protein Intake ◽

Meat Products ◽

Food Sources ◽

Animal Origin ◽

Total Protein Intake ◽

Fish And Shellfish ◽

Milk And Dairy Products

Diet in the first years of life is an important factor in growth and development. Dietary protein is a critical macronutrient that provides both essential and nonessential amino acids required for sustaining all body functions and procedures, providing the structural basis to maintain life and healthy development and growth in children. In this study, our aim was to describe the total protein intake, type and food sources of protein, the adequacy to the Population Reference Intake (PRI) for protein by the European Food Safety Authority (EFSA), and the Recommended Dietary Allowance (RDA) by the Institute of Medicine (IoM). Furthermore, we analyzed whether the consumption of dairy products (including regular milk, dairy products, or adapted milk formulas) is associated with nutrient adequacy and the contribution of protein to diet and whole dietary profile in the two cohorts of the EsNuPI (in English, Nutritional Study in the Spanish Pediatric Population) study; one cohort was representative of the Spanish population from one to < 10 years old (n = 707) (Spanish reference cohort, SRS) who reported consuming all kinds of milk and one was a cohort of the same age who reported consuming adapted milk over the last year (including follow-on formula, growing up milk, toddler’s milk, and enriched and fortified milks) (n = 741) (adapted milk consumers cohort, AMS). The children of both cohorts had a high contribution from protein to total energy intake (16.79% SRS and 15.63% AMS) and a high total protein intake (60.89 g/day SRS and 53.43 g/day AMS). We observed that protein intake in Spanish children aged one to < 10 years old was above the European and international recommendations, as well as the recommended percentages for energy intakes. The main protein sources were milk and dairy products (28% SRS and 29% AMS) and meat and meat products (27% SRS and 26% AMS), followed by cereals (16% SRS and 15% AMS), fish and shellfish (8% in both cohorts), eggs (5% SRS and 6% AMS), and legumes (4% in both cohorts). In our study population, protein intake was mainly from an animal origin (meat and meat products, milk and dairy products, fish and shellfish, and eggs) rather than from a plant origin (cereals and legumes). Future studies should investigate the long-term effect of dietary protein in early childhood on growth and body composition, and whether high protein intake affects health later in life.

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Physical Activity and Sedentary Behavior’s Association With Body Weight in Korean Adolescents

International Journal of Sport Nutrition and Exercise Metabolism ◽

10.1123/ijsnem.23.1.1 ◽

2013 ◽

Vol 23 (1) ◽

pp. 1-10 ◽

Cited By ~ 4

Author(s):

Wi-Young So

Keyword(s):

Physical Activity ◽

Body Mass Index ◽

Body Weight ◽

Muscle Strength ◽

Sedentary Behavior ◽

Weight Status ◽

The Other ◽

Web Based ◽

Korean Adolescents ◽

Study Results

The purpose of this study was to examine whether physical activity (PA) and sedentary behavior were related to weight status in Korean adolescents. A total of 72,399 students in Grades 7–12 participated in the 5th Korea Youth Risk Behavior Web-Based Survey (KYRBWS-V) project in 2009. Body mass index, PA, and sedentary behavior were assessed by the KYRBWS-V. The study results show that boys had higher PA and less sedentary behavior than did girls (p < .01). The amounts of vigorous PA (p < .001), moderate PA (p < .001), moderate PA for at least 60 min (p < .001), exercise for muscle strength (p < .001), walking on a weekday (p < .001), and walking on a weekend (p = .012) were less in boys who were more obese than the other boys. The time spent sitting on a weekday (p < .001) and weekend (p < .001) was higher in more obese individuals in boys. Exercise for muscle strength (p = .011) was less in girls who were more obese. Time spent sitting on a weekday (p = .005) and weekend (p < .001) was higher in more obese individuals in girls. However, vigorous PA (p < .001), moderate PA (p < .001), moderate PA for at least 60 min (p = .003), and walking on a weekday (p < .001) were higher for the more obese girls. PA and sedentary behavior could be independent factors that reduce or prevent obesity in Korean adolescents.

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Protein intake per day and at each daily meal and skeletal muscle mass declines among older community dwellers in Japan

Public Health Nutrition ◽

10.1017/s1368980019002921 ◽

2019 ◽

Vol 23 (6) ◽

pp. 1090-1097 ◽

Cited By ~ 8

Author(s):

Rei Otsuka ◽

Yuki Kato ◽

Chikako Tange ◽

Yukiko Nishita ◽

Makiko Tomida ◽

...

Keyword(s):

Skeletal Muscle ◽

Muscle Mass ◽

Total Protein ◽

Protein Intake ◽

Skeletal Muscle Mass ◽

Total Protein Intake ◽

Dietary Records ◽

Logistic Modelling ◽

Community Dwellers

AbstractObjective:To examine associations between protein intake per day and at different meals and skeletal muscle mass declines.Design:Two-year prospective cohort study among older community dwellers.Setting:National Institute for Longevity Sciences–Longitudinal Study of Aging (NILS-LSA) in Japan.Participants:Older men (n 292) and women (n 363) aged 60–87 years who participated in the baseline (2006–2008) and follow-up studies (2008–2010) of NILS-LSA and did not exhibit low skeletal muscle mass at baseline. Muscle mass was assessed using dual-energy X-ray absorptiometry at baseline and follow-up. Low muscle mass was defined as skeletal muscle mass index <7·0 kg/m2 for men and <5·4 kg/m2 for women at follow-up. Daily protein intake and protein intake at each meal were calculated from 3 d dietary records at baseline and sex-stratified tertiles were determined.Results:Mean (sd) protein intake at breakfast, lunch and dinner was 22·7 (7·8), 26·7 (9·3) and 37·4 (10·5) g for men and 19·3 (6·3), 23·2 (7·3) and 28·5 (7·0) g for women, respectively. After adjusting for age, baseline skeletal muscle mass and other confounders in logistic modelling, greater total protein intake was associated with lower prevalence of skeletal muscle mass decline among men at follow-up (P = 0·024). Particularly, the OR (95 % CI) for high lunchtime protein intake was low (0·11 (0·02, 0·61); P = 0·01). No significant association between total protein intake and prevalence of skeletal muscle mass decline was found among women.Conclusions:High total protein intake, particularly at lunchtime, is associated with retention of skeletal muscle mass in men.

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Pattern of Dietary Intake and Consumption of Street Foods among Nigerian Students

Nutrition and Health ◽

10.1177/026010609801200405 ◽

1998 ◽

Vol 12 (4) ◽

pp. 247-256 ◽

Cited By ~ 8

Author(s):

Clara R.B. Oguntona ◽

M.A. Razaq ◽

Tolulope T. Akintola

Keyword(s):

Vitamin A ◽

Total Protein ◽

Protein Intake ◽

Nutrient Intake ◽

Male And Female ◽

Total Protein Intake ◽

Food And Nutrient Intake ◽

Diet Recall ◽

Nigerian Students ◽

Female Subjects

An assessment of the food and nutrient intake of 81 male and 35 female University students was conducted using structured questionnaires and 24-hr. diet recall procedures. The contribution of Street Foods (SF) to their nutrients intakes was also assessed. The major dietary sources of nutrients for both groups were Cereals, Roots and Tubers and Legumes. Energy intake by males (9.81 MJ or 83.9% RDA) was significantly (p < 0.05) higher than intake (8.59 MJ or 81.8% RDA) by female subjects. Street Foods contributed 50.3% and 48.3% of energy to male and female subjects respectively. Total Protein intake approximated RDA for both groups although on average only 25% of this was from animal sources. Intakes of minerals and vitamins were generally below RDA except for Calcium and vitamin A. Female subjects had significantly higher intakes of Iron, Thiamin and vitamin A than males. Overall Street food's contribution to RDA ranged from 23.0% (Iron in males) to 80.0% (vitamin A, also in males).

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The Association of Protein Intake and Bone Outcomes in Midwestern Post-menopausal Women

Current Developments in Nutrition ◽

10.1093/cdn/nzab038_027 ◽

2021 ◽

Vol 5 (Supplement_2) ◽

pp. 415-415

Author(s):

Mariah Jackson ◽

Fang Niu ◽

Lynette Smith ◽

Laura Bilek ◽

Nancy Waltman ◽

...

Keyword(s):

Amino Acid ◽

Total Protein ◽

Protein Intake ◽

Secondary Analysis ◽

Dietary Factors ◽

Whole Body ◽

Total Protein Intake ◽

Menopausal Women ◽

Hip Bmd ◽

Post Menopausal

Abstract Objectives Determine the extent to which baseline protein and amino acid intake is independently associated with measures of baseline bone outcomes in a cohort of post-menopausal women. Methods This study was a secondary analysis of participants in the Heartland Osteoporosis Prevention Study (HOPS) randomized controlled trial evaluating the effect of different osteoporosis interventions on post-menopausal women in the Midwest. Diets were evaluated via a Harvard Willett Semi-quantitative Food Frequency Questionnaire (FFQ) at baseline. Dual energy x-ray absorptiometry (DXA) scans were used to collect bone mineral density (BMD) and trabecular bone score (TBS) measurements. Single and multivariate linear regression of total protein intake (g/d and g/kg/d), proline (g), and lysine (g) with BMD and TBS locations were conducted. Student T-tests were used to compare the bone outcome differences between groups with protein intake above and below 1.2g/kg. Results There were 249 participants available for analysis. Mean total protein intake was 82.7 g with 62% consuming < 1.2/kg/d. After adjustment, for every additional gram intake of protein, lysine and proline, Hip BMD increased (Protein: β = 0.0005, P = 0.047; Lysine: β = 0.006, P = 0.04; Proline: β = 0.008, P = 0.04). Whole body BMD and TBS-1 were significantly associated with proline intake (Whole body BMD: β = 0.008, P = 0.04; TBS-1: β = −0.007, P = 0.02). After adjustment, women who consumed protein above 1.2 g/kg, had a Hip BMD 0.03 g/cm2 higher than those who consume protein below 1.2 g/kg (β = 0.03, P = 0.046). Lumbar BMD, Total L1-L4 BMD, TBS-2, TBS-3, TBS-4, and Total TBS were not significantly associated with protein or amino acid intakes after adjustment of covariates. Conclusions Higher protein intakes were associated with higher Hip BMD. Proline may play a controversial role in bone outcomes. Future research should investigate the role of dietary factors on BMD vs TBS. Funding Sources The original research was funded by the National Institute of Nursing Research of the National Institutes of Health under award number R01NR015029. No additional funding was used for the secondary analysis.

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