scholarly journals The Antidiabetic Effect of Grape Pomace Polysaccharide-Polyphenol Complexes

Nutrients ◽  
2021 ◽  
Vol 13 (12) ◽  
pp. 4495
Author(s):  
Filipa Campos ◽  
Andreia F. Peixoto ◽  
Pedro A. R. Fernandes ◽  
Manuel A. Coimbra ◽  
Nuno Mateus ◽  
...  
Keyword(s):  
Fruits And Vegetables ◽  
Metabolic Diseases ◽  
Liquid Extraction ◽  
Grape Pomace ◽  
Hot Water ◽  
Total Phenolic ◽  
Pectic Polysaccharides ◽  
Antidiabetic Effect ◽  
Solid Liquid Extraction ◽  
Solid Liquid

Type 2 diabetes mellitus (T2DM) is one of the most prevalent chronic metabolic diseases of the 21st century. Nevertheless, its prevalence might be attenuated by taking advantage of bioactive compounds commonly found in fruits and vegetables. This work is focused on the recovery of polyphenols and polysaccharide–polyphenol conjugates from grape pomace for T2DM management and prevention. Bioactives were extracted by solid–liquid extraction and by pressurized hot water extraction (PHWE). Polyphenolic fraction recovered by PHWE showed the highest value for total phenolic content (427 mg GAE.mg−1), mainly anthocyanins and proanthocyanidins, and higher antioxidant activity compared to the fraction recovered by solid–liquid extraction. Polysaccharide–polyphenol conjugates comprehended pectic polysaccharides to which approximately 108 mg GAE of phenolic compounds (per mg fraction) were estimated to be bound. Polyphenols and polysaccharide–polyphenol conjugates exhibited distinct antidiabetic effects, depending on the extraction methodologies employed. Extracts were particularly relevant in the inhibition of a-glucosidase activity, with free polyphenols showing an IC50 of 0.47 mg.mL−1 while conjugates showed an IC50 of 2.7, 4.0 and 5.2 mg.mL−1 (solid–liquid extraction, PHWE at 95 and 120°C, respectively). Antiglycation effect was more pronounced for free polyphenols recovered by PHWE, while the attenuation of glucose uptake by Caco-2 monolayers was more efficient for conjugates obtained by PHWE. The antidiabetic effect of grape pomace bioactives opens new opportunities for the exploitation of these agri-food wastes in food nutrition, the next step towards reaching a circular economy in grape products.

scholarly journals Continuous or Batch Solid-Liquid Extraction of Antioxidant Compounds from Seeds of Sterculia apetala Plant and Kinetic Release Study

Molecules ◽  
2018 ◽  
Vol 23 (7) ◽  
pp. 1759 ◽  
Author(s):  
Federica Mosca ◽  
Gádor Hidalgo ◽  
Juliana Villasante ◽  
María Almajano
Keyword(s):  
Diffusion Rate ◽  
Effective Diffusion ◽  
Antioxidant Properties ◽  
Chemical Species ◽  
Liquid Extraction ◽  
Process Time ◽  
Total Phenolic ◽  
Antioxidant Compounds ◽  
Solid Liquid Extraction ◽  
Solid Liquid

This work has been intended to investigate the antioxidant properties of compounds extracted from seeds of Sterculia apetala (a plant from Central America) in order to add further results to the relatively poor existing literature on the beneficial properties of this plant. Different extraction methodologies were used such as batch or continuous extraction conditions using water or ethanol 50% as solvents. The kinetic study has allowed estimation of the effective diffusion coefficients in a continuous solid-liquid extraction, highlighting the strict dependence of the diffusion rate and temperature and kind of solvent, showing the highest diffusion rate with ethanol 50% at 60 °C. The comparison between different techniques and two solvents led to the selection of water as the best extraction solvent while batch mechanically-agitated extraction was the most efficient mode, with the benefits of use of an environmental-friendly solvent and reduction in process time to achieve a high amount of extracted phenolic compounds. The analysis techniques used were ABTS and Folin-Ciocalteau methods to investigate the antioxidant activity and quantify the Total Phenolic Content (TPC) respectively. Also, different fatty acids were extracted from Sterculia apetala seeds and analysed by Gas Chromatography in order to quantify other interesting chemical species besides antioxidants.

Supercritical fluid and solid–liquid extraction of phenolic antioxidants from grape pomace: a comparative study

2006 ◽  
Vol 226 (1-2) ◽  
pp. 199-205 ◽  
Author(s):  
Manuel Pinelo ◽  
Alejandro Ruiz-Rodríguez ◽  
Jorge Sineiro ◽  
Francisco J. Señoráns ◽  
Guillermo Reglero ◽  
...  
Keyword(s):  
Comparative Study ◽  
Supercritical Fluid ◽  
Liquid Extraction ◽  
Grape Pomace ◽  
Phenolic Antioxidants ◽  
Solid Liquid Extraction ◽  
Solid Liquid

scholarly journals Valorization of Bilberry (Vaccinium myrtillus L.) Pomace by Enzyme-Assisted Extraction: Process Optimization and Comparison with Conventional Solid-Liquid Extraction

Antioxidants ◽  
2021 ◽  
Vol 10 (5) ◽  
pp. 773
Author(s):  
Michail Syrpas ◽  
Egle Valanciene ◽  
Ernesta Augustiniene ◽  
Naglis Malys
Keyword(s):  
Antioxidant Capacity ◽  
Liquid Extraction ◽  
Vaccinium Myrtillus ◽  
Water Soluble ◽  
Total Phenolic ◽  
Assisted Extraction ◽  
Solid Liquid Extraction ◽  
Solid Liquid ◽  
Vaccinium Myrtillus L ◽  
Enzyme Assisted Extraction

Bilberry (Vaccinium myrtillus L.) pomace contains a significant amount of polyphenols and can serve as a basis for food additives, nutraceuticals, and functional foods. Although various techniques can be employed to recover bioactive fractions from berry pomaces, data on enzyme-assisted extraction (EAE) of bilberry pomace are rather scarce. This study aimed to optimize critical EAE parameters using Viscozyme L to obtain a high-yield extract with enhanced antioxidant capacity. Central composite design and response surface methodology evaluating the effect of four independent variables, namely, pH, temperature, extraction time, and enzyme concentration on three responses, were employed to define optimal EAE conditions. Under the optimal conditions (pH: 4.5, temperature 46 °C, 1 h of extraction, and 2 active units (AU) of Viscozyme L/g of pomace), EAE yielded 56.15 g/ 100 g DW of the water-soluble fraction. Comparison with conventional maceration indicated that EAE, besides the yield, significantly increased the in vitro antioxidant capacity measured by the total phenolic content, ABTS, ORAC, and CUPRAC assays. Moreover, an increase was observed for the measured mono- and disaccharide as well as anthocyanin content. Overall, this study demonstrates the improved efficiency of EAE over conventional solid–liquid extraction to recover fractions with a higher yield and enhanced functional properties in a fast and sustainable manner.

scholarly journals Protective Effect of Cyclically Pressurized Solid–Liquid Extraction Polyphenols from Cagnulari Grape Pomace on Oxidative Endothelial Cell Death

Molecules ◽  
2018 ◽  
Vol 23 (9) ◽  
pp. 2105 ◽  
Author(s):  
Anna Posadino ◽  
Grazia Biosa ◽  
Hatem Zayed ◽  
Haissam Abou-Saleh ◽  
Annalisa Cossu ◽  
...  
Keyword(s):  
Hydrogen Peroxide ◽  
Cell Death ◽  
Biological Activities ◽  
Liquid Extraction ◽  
Grape Pomace ◽  
Ros Generation ◽  
Extraction Technology ◽  
Fluorescence Measurements ◽  
Solid Liquid Extraction ◽  
Solid Liquid

The aim of this work is the evaluation of a green extraction technology to exploit winery waste byproducts. Specifically, a solid–liquid extraction technology (Naviglio Extractor®) was used to obtain polyphenolic antioxidants from the Cagnulari grape marc. The extract was then chemically characterized by spectrophotometric analysis, high-performance liquid chromatography, and mass spectrometry, revealing a total polyphenol content of 4.00 g/L ± 0.05, and the presence of anthocyanins, one of the most representative groups among the total polyphenols in grapes. To investigate potential biological activities of the extract, its ability to counteract hydrogen peroxide-induced oxidative stress and cell death was assessed in primary human endothelial cells. The 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) test, used to assess potential extract cytotoxicity, failed to show any deleterious effect on cultured cells. Fluorescence measurements, attained with the intracellular reactive oxygen species (ROS) probe 2′,7′-dichlorodihydrofluorescein diacetate (H2DCF-DA), revealed a strong antioxidant potential of the marc extract on the used cells, as indicated by the inhibition of the hydrogen peroxide-induced ROS generation and the counteraction of the oxidative-induced cell death. Our results indicate the Naviglio extraction, as a green technology process, can be used to exploit wine waste to obtain antioxidants which can be used to produce enriched foods and nutraceuticals high in antioxidants.

scholarly journals Influence of Brewer’s Spent Grain Compounds on Glucose Metabolism Enzymes

Nutrients ◽  
2021 ◽  
Vol 13 (8) ◽  
pp. 2696
Author(s):  
Daniela Becker ◽  
Tamara Bakuradze ◽  
Marcel Hensel ◽  
Simone Beller ◽  
Carolina Corral Yélamos ◽  
...  
Keyword(s):  
Glucose Metabolism ◽  
Liquid Extraction ◽  
Total Phenolic ◽  
Bioactive Substances ◽  
Brewer’S Spent Grain ◽  
Dpp Iv ◽  
Metabolism Enzymes ◽  
Spent Grain ◽  
Solid Liquid Extraction ◽  
Solid Liquid

With a yearly production of about 39 million tons, brewer’s spent grain (BSG) is the most abundant brewing industry byproduct. Because it is rich in fiber and protein, it is commonly used as cattle feed but could also be used within the human diet. Additionally, it contains many bioactive substances such as hydroxycinnamic acids that are known to be antioxidants and potent inhibitors of enzymes of glucose metabolism. Therefore, our study aim was to prepare different extracts—A1-A7 (solid-liquid extraction with 60% acetone); HE1-HE6 (alkaline hydrolysis followed by ethyl acetate extraction) and HA1-HA3 (60% acetone extraction of alkaline residue)—from various BSGs which were characterized for their total phenolic (TPC) and total flavonoid (TFC) contents, before conducting in vitro studies on their effects on the glucose metabolism enzymes α-amylase, α-glucosidase, dipeptidyl peptidase IV (DPP IV), and glycogen phosphorylase α (GPα). Depending on the extraction procedures, TPCs ranged from 20–350 µg gallic acid equivalents/mg extract and TFCs were as high as 94 µg catechin equivalents/mg extract. Strong inhibition of glucose metabolism enzymes was also observed: the IC50 values for α-glucosidase inhibition ranged from 67.4 ± 8.1 µg/mL to 268.1 ± 29.4 µg/mL, for DPP IV inhibition they ranged from 290.6 ± 97.4 to 778.4 ± 95.5 µg/mL and for GPα enzyme inhibition from 12.6 ± 1.1 to 261 ± 6 µg/mL. However, the extracts did not strongly inhibit α-amylase. In general, the A extracts from solid-liquid extraction with 60% acetone showed stronger inhibitory potential towards a-glucosidase and GPα than other extracts whereby no correlation with TPC or TFC were observed. Additionally, DPP IV was mainly inhibited by HE extracts but the effect was not of biological relevance. Our results show that BSG is a potent source of α-glucosidase and GPα inhibitors, but further research is needed to identify these bioactive compounds within BSG extracts focusing on extracts from solid-liquid extraction with 60% acetone.

Rotation planar extraction and medium-pressure solid-liquid extraction of onion (Allium cepa)

2003 ◽  
Vol 16 (1) ◽  
pp. 66-70 ◽  
Author(s):  
Irena Vovk ◽  
Breda Simonovska ◽  
Samo Andrenšek ◽  
Teijo Yrjönen ◽  
Pia Vuorela ◽  
...  
Keyword(s):  
Allium Cepa ◽  
Liquid Extraction ◽  
Medium Pressure ◽  
Solid Liquid Extraction ◽  
Solid Liquid

MASS TRANSFER IN SOLID-LIQUID EXTRACTION AT HIGH SOLUTE CONCENTRATIONS

2018 ◽  
Vol 17 (4) ◽  
pp. 827-839
Author(s):  
Eugenia Teodora Iacob Tudose ◽  
Marcela Popa ◽  
Ioan Mamaliga
Keyword(s):  
Mass Transfer ◽  
Liquid Extraction ◽  
Solid Liquid Extraction ◽  
Solid Liquid

Ultrasound Assisted Solid-Liquid Extraction Devices - Harnessing Active Ingredients from Plant materials – Overview and Analysis

2020 ◽  
Vol 13 ◽  
Author(s):  
Venkatasubramanian Sivakumar
Keyword(s):  
Mass Transfer ◽  
Cell Wall ◽  
Plant Cell ◽  
Plant Cell Wall ◽  
Liquid Extraction ◽  
Active Ingredients ◽  
Plant Materials ◽  
Ultrasound Assisted ◽  
Solid Liquid Extraction ◽  
Solid Liquid

Background: In the growing environmental concern use of natural products, efficient processes and devices are necessary. Solid-Liquid extraction of active Ingredients from Plant materials is one of the important unit operations in Chemical Engineering and need to be enhanced. Objectives: Since, these active ingredients are firmly bound to the plant cell wall membrane, which pose mass-transfer resistance and need to get detached through the use of suitable process intensification tools such as ultrasound and suitable devices. Therefore, detailed analysis and review is essential on development made in this area through Publications and Patents. Hence, the present paper illustrates the development of ultrasound assisted device for solid-liquid extraction are presented in this paper. Methods: Advantages such as % Yield, Reduction in extraction time, use of ambient conditions, better process control, avoidance or minimizing multi stage extraction could be achieved due to the use of ultrasound in extraction as compared to conventional processes. Conclusions: Use of ultrasound to provide significant improvements in the extraction of Vegetable tannins, Natural dyes for application in Leather processing has been demonstrated and reported earlier. These enhancement could be possible through various effects of ultrasound such as better flow of solvents through micro-jet formation, mass transfer enhancement due to rupture of plant cell wall membranes through acoustic cavitation, better leaching due to micro-mixing and acoustic streaming effects. This approach would minimize material wastage; thereby, leading to eco-conservation of plant materials, which is very much essential for better environment. Hence, various methods and design for application of ultrasound assisted solid-liquid extractor device are necessary.

Superiority of polymeric scavenger over its hexapodal monomer towards efficient ReO4- removal in water

2021 ◽  
Author(s):  
Pradyut Ghosh ◽  
Rajib Ghosh ◽  
Tamal Kanti Ghosh
Keyword(s):  
Aqueous Medium ◽  
Liquid Extraction ◽  
Efficient Removal ◽  
Solid Liquid Extraction ◽  
Solid Liquid

A new imidazolium functionalized hexapodal polymeric receptor, [PHIm-Br] showed selective and efficient removal (>99%) of perrhenate (ReO4-), from 100% aqueous medium via solid-liquid extraction which was 13% higher as compared...

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