scholarly journals Genotypic Comparison between Streptococcus suis Isolated from Pigs and Humans in Thailand

Pathogens ◽  
2020 ◽  
Vol 9 (1) ◽  
pp. 50 ◽  
Author(s):  
Anusak Kerdsin ◽  
Dan Takeuchi ◽  
Aniroot Nuangmek ◽  
Yukihiro Akeda ◽  
Marcelo Gottschalk ◽  
...  
Keyword(s):  
Risk Factor ◽  
Risk Group ◽  
Streptococcus Suis ◽  
Prevalence Rates ◽  
Swine Industry ◽  
Carriage Rate ◽  
Zoonotic Pathogen ◽  
Pork Products ◽  
Economic Significance ◽  
Sequence Types

Streptococcus suis is a zoonotic pathogen of economic significance to the swine industry. The number of infected cases is increasing in humans worldwide. In this study, we determined the prevalence and diversity of S. suis carriage in slaughterhouse pigs in Phayao province, Thailand, where an outbreak occurred in 2007. The overall S. suis carriage rate was 35.2% among slaughterhouse pigs. The prevalence rates of serotypes 2 and 14 (the major serotypes infected in humans) were 6.7% and 2.6%, respectively. In both serotypes, 70.4% of isolates of serotypes 2 and 14 revealed sequence types and pulsotypes identical to human isolates in Thailand. It is suggested that pathogenic strains of S. suis are a risk factor for occupational exposure to pigs or the consumption of raw pork products. Food safety, hygiene, and health education should be encouraged to reduce the risk group.

Genetic analysis and pathogenicity of different sequence types of Streptococcus suis isolated from pigs in southern China

2020 ◽  
Vol 367 (6) ◽  
Author(s):  
Ling Peng ◽  
Mengting Lin ◽  
Zishu Huang ◽  
Sitao Guo ◽  
Hao Sun ◽  
...  
Keyword(s):  
Multilocus Sequence Typing ◽  
Associated Factors ◽  
Southern China ◽  
Streptococcus Suis ◽  
Genome Sequences ◽  
Swine Industry ◽  
Highly Pathogenic ◽  
Genetic Typing ◽  
Typing Methods ◽  
Sequence Types

ABSTRACT In this study, 52 Streptococcus suis isolates from pigs in southern China were divided into four known sequence types (STs) and six new STs, using multilocus sequence typing. Ten representative isolates were selected from 10 STs for the analysis of whole genome sequences. Virulence was assessed in 10 isolates, which were classified into three pathogenic groups. The prevalence of virulence-associated factors in the moderately pathogenic group was higher than that in the highly pathogenic group. The isolates from ST1 complex and serotype 2 were allocated to the moderately pathogenic group, while the isolates from the highly pathogenic group belonged to the non-ST1 complex and non-serotype 2. Three clusters were obtained based on multilocus sequence typing sequences: cluster III isolates from the nasal cavity of healthy pigs were classified into the highly pathogenic group and showed many peculiarities compared with cluster I and II isolates in virulence genotypes, genetic typing and pathogenesis, indicating a potential independent evolutionary line. Our results suggest that S. suis infections in China are becoming more complicated with constantly mutating isolates, which makes it difficult to distinguish their virulence by recognized typing methods. Thus, increased investigation and monitoring of these infections should be a priority for the swine industry in China.

scholarly journals Update on the Mechanisms of Antibiotic Resistance and the Mobile Resistome in the Emerging Zoonotic Pathogen Streptococcus suis

2021 ◽  
Vol 9 (8) ◽  
pp. 1765
Author(s):  
Manon Dechêne-Tempier ◽  
Corinne Marois-Créhan ◽  
Virginie Libante ◽  
Eric Jouy ◽  
Nathalie Leblond-Bourget ◽  
...  
Keyword(s):  
Antibiotic Resistance ◽  
Resistance Genes ◽  
Bacterial Species ◽  
Antibiotic Resistance Genes ◽  
Streptococcus Suis ◽  
Resistance Mechanisms ◽  
Economic Losses ◽  
Swine Production ◽  
Swine Industry ◽  
Zoonotic Pathogen

Streptococcus suis is a zoonotic pathogen causing important economic losses in swine production. The most commonly used antibiotics in swine industry are tetracyclines, beta-lactams, and macrolides. Resistance to these antibiotics has already been observed worldwide (reaching high rates for macrolides and tetracyclines) as well as resistance to aminoglycosides, fluoroquinolones, amphenicols, and glycopeptides. Most of the resistance mechanisms are encoded by antibiotic resistance genes, and a large part are carried by mobile genetic elements (MGEs) that can be transferred through horizontal gene transfer. This review provides an update of the resistance genes, their combination in multidrug isolates, and their localization on MGEs in S. suis. It also includes an overview of the contribution of biofilm to antimicrobial resistance in this bacterial species. The identification of resistance genes and study of their localization in S. suis as well as the environmental factors that can modulate their dissemination appear essential in order to decipher the role of this bacterium as a reservoir of antibiotic genes for other species.

scholarly journals Streptococcus suis Isolates—Serotypes and Susceptibility to Antimicrobials in Terms of Their Use on Selected Repopulated Czech Pig Farms

Pathogens ◽  
2021 ◽  
Vol 10 (10) ◽  
pp. 1314
Author(s):  
Ján Matiašovic ◽  
Kateřina Nedbalcová ◽  
Marek Žižlavský ◽  
Petr Fleischer ◽  
Lucie Pokludová ◽  
...  
Keyword(s):  
Streptococcus Suis ◽  
Antimicrobial Treatment ◽  
Beta Lactam ◽  
Swine Industry ◽  
The Czech Republic ◽  
The Past ◽  
Beta Lactam Antibiotics ◽  
Pig Farms ◽  
History Of ◽  
Sequence Types

Streptococcus suis represents a primary health problem (such as meningitis, septicemia and arthritis in piglets and fatteners) in the swine industry worldwide and also an emerging zoonotic pathogen. In the Czech Republic, many pig farms repopulated their herds over the past decades to reduce morbidity and minimize treatment. The study analysed serotypes, sequence types and antimicrobial susceptibility in 39 S. suis isolates obtained from organs of diseased pigs from selected 16 repopulated farms with a history of S. suis-associated diseases and routine antimicrobial treatment with tulathromycin and/or amoxicillin. The analysis revealed diversity of collected isolates with regular occurrence of more than three serotypes per farm. The serotypes identified were 1/2 and 7, each in six isolates, followed by serotype 2 and 3 found in five isolates each, other serotypes were less frequent. Seven isolates were not typable by multiplex PCR and we also found sequence type of unknown type in thirteen isolates. The majority of S. suis isolates were resistant to clindamycin (n = 31), tetracycline (n = 29) and tilmicosin and tulathromycin (n = 28). On the other hand, with the exception of two isolates that were intermediately susceptible to penicillin and ampicillin, all isolates were susceptible to all three tested subgroups of beta-lactam antibiotics.

scholarly journals Latest developments on Streptococcus suis: an emerging zoonotic pathogen: part 1

2014 ◽  
Vol 9 (4) ◽  
pp. 441-444 ◽  
Author(s):  
Mariela Segura ◽  
Han Zheng ◽  
Astrid de Greeff ◽  
George F Gao ◽  
Daniel Grenier ◽  
...  
Keyword(s):  
Streptococcus Suis ◽  
Zoonotic Pathogen

scholarly journals Epidemiological situation of bovine brucellosis in the State of Paraiba, Brazil

2016 ◽  
Vol 37 (5Supl2) ◽  
pp. 3403 ◽  
Author(s):  
Inácio José Clementino ◽  
Ricardo Augusto Dias ◽  
Marcos Amaku ◽  
Fernando Ferreira ◽  
Evelise Oliveira Telles ◽  
...  
Keyword(s):  
Risk Factor ◽  
Odds Ratio ◽  
The State ◽  
Prevalence Rates ◽  
Risk Of Infection ◽  
Bovine Brucellosis ◽  
Serum Samples ◽  
The Rose ◽  
Positive Results ◽  
Epidemiological Situation

This study was performed to characterize the epidemiological status of brucellosis in the State of Paraíba, Brazil. The State was divided into three regions. Herds were randomly sampled in each region and a pre-established number of animals were sampled in each of these herds. A total of 3,489 serum samples from 674 herds were collected. In each herd, an epidemiological questionnaire was conducted. This questionnaire focused on herd traits, as well as husbandry and sanitary practices that could be associated with the risk of infection. The serum samples were screened for antibodies against Brucella spp. by the Rose-Bengal Test (RBT), and all positive sera were confirmed by the 2-mercaptoethanol test (2-ME). The herd was considered positive if at least one animal had positive results for both the RBT and the 2-ME test. The prevalence rates of infected herds and animals in the State were 4.6% [3.2-6.5%] and 2.5% [1.1-3.9%], respectively. The prevalence rates of infected herds and animals in the regions were, respectively: region 1, 3.2% [1.5-6.6%] and 1.7% [0.5-5.7%]; region 2, 2.2% [0.9-5.2%] and 0.7% [0.3-1.7%]; and region 3, 7.9% [5.0-12.2%] and 3.2% [1.6-6.3%]. The risk factor (odds ratio, OR) associated with the presence of the infection was Zebuine as the predominant breed (OR=12.30 [1.32-114.64]).

scholarly journals Low Body Mass Index Can Identify Majority of Osteoporotic Inflammatory Bowel Disease Patients Missed by Current Guidelines

2012 ◽  
Vol 2012 ◽  
pp. 1-6 ◽  
Author(s):  
Ashish Atreja ◽  
Ashish Aggarwal ◽  
Angelo A. Licata ◽  
Bret A. Lashner
Keyword(s):  
Risk Factors ◽  
Inflammatory Bowel Disease ◽  
At Risk ◽  
Risk Factor ◽  
Risk Group ◽  
Low Bmi ◽  
Inflammatory Bowel ◽  
Provider Adherence ◽  
Not At Risk ◽  
Current Guidelines

Background. Patients with inflammatory bowel disease (IBD) are at high risk of developing osteoporosis. Our objective was to determine the usefulness of IBD guidelines in identifying patients at risk for developing osteoporosis.Methods. We utilized institutional repository to identify patients seen in IBD center and extracted data on demographics, disease history, conventional, and nonconventional risk factors for osteoporosis and Dual Energy X-ray Absorptiometry (DXA) findings.Results. 59% of patients (1004/1703) in our IBD cohort had at least one risk factor for osteoporosis screening. DXA was documented in 263 patients with indication of screening (provider adherence, 26.2%), and of these, 196 patients had DXA completed (“at-risk” group). Ninety-five patients not meeting guidelines-based risk factors also had DXA completed (“not at-risk” group). 139 (70.9%) patients in “at-risk” group had low BMD, while 51 (53.7%) of “not-at-risk” patients had low BMD. Majority of the patients with osteoporosis (83.3%) missed by the current guidelines had low BMI. Multivariate logistic regression analysis showed that low BMI was the strongest risk factor for osteoporosis (OR 3.07; 95% CI, 1.47–6.42;P=0.003).Conclusions. Provider adherence to current guidelines is suboptimal. Low BMI can identify majority of the patients with osteoporosis that are missed by current guidelines.

scholarly journals Meningite Bacteriana Aguda como Doença Ocupacional

2014 ◽  
Vol 27 (4) ◽  
pp. 519
Author(s):  
Diana Seixas ◽  
Ana Lebre ◽  
Pedro Crespo ◽  
Eugénia Ferreira ◽  
José Eduardo Serra ◽  
...  
Keyword(s):  
Occupational Exposure ◽  
Alcohol Consumption ◽  
Occupational Diseases ◽  
Streptococcus Suis ◽  
Acute Bacterial Meningitis ◽  
Streptococcal Infections ◽  
Past Medical History ◽  
Zoonotic Pathogen ◽  
Worldwide Distribution ◽  
Microbiological Examination

Streptococcus suis is a zoonotic pathogen with worldwide distribution, responsible for more than 700 human cases globally reported. This infection affects mostly men, exposed to pig or pork, which leads to its usual classification as an occupational disease. We report a case of acute bacterial meningitis in a 44 years old male. According to his past medical history, the patient had alcohol consumption and worked in a restaurant as a piglet griller. Microbiological examination of blood and CSF revealed S. suis. After 14 days of ceftriaxone the patient fully recovered. The authors review the clinical reports previously described in Portugal. In all of them was possible to identify risk exposition to pork. We alert to this microorganism’s importance in Portugal where it is probably underdiagnosed.<br /><strong>Keywords:</strong> Occupational Diseases; Occupational Exposure; Meningitis, Bacterial; Streptococcal Infections; Streptococcus suis; Portugal.

scholarly journals Effect of Mupirocin Treatment on Nasal, Pharyngeal, and Perineal Carriage of Staphylococcus aureus in Healthy Adults

2005 ◽  
Vol 49 (4) ◽  
pp. 1465-1467 ◽  
Author(s):  
Heiman F. L. Wertheim ◽  
Jeroen Verveer ◽  
Hélène A. M. Boelens ◽  
Alex van Belkum ◽  
Henri A. Verbrugh ◽  
...  
Keyword(s):  
Staphylococcus Aureus ◽  
Clinical Trials ◽  
Risk Factor ◽  
Fragment Length ◽  
Protein A ◽  
Nasal Carriage ◽  
Common Phenomenon ◽  
Carriage Rate ◽  
Length Polymorphisms ◽  
Restriction Fragment Length

ABSTRACT Nasal carriage of Staphylococcus aureus is an important risk factor for S. aureus infections. Mupirocin nasal ointment is presently the treatment of choice for decolonizing the anterior nares. However, recent clinical trials show limited benefit from mupirocin prophylaxis in preventing nosocomial S. aureus infections, probably due to (re)colonization from extranasal carriage sites. Therefore, we studied the effectiveness of mupirocin nasal ointment treatment on the dynamics of S. aureus nasal and extranasal carriage. Twenty noncarriers, 26 intermittent carriers, and 16 persistent carriers had nasal, throat, and perineum samples taken 1 day before and 5 weeks after mupirocin treatment (twice daily for 5 days) and assessed for growth of S. aureus. The identities of cultured strains were assessed by restriction fragment length polymorphisms of the coagulase and protein A genes. The overall carriage rate (either nasal, pharyngeal, or perineal carrier or a combination) was significantly reduced after mupirocin treatment from 30 to 17 carriers (P = 0.003). Of the 17 carriers, 10 (60%) were still colonized with their old strain, 6 (35%) were colonized with an exogenous strain, and 1 (5%) was colonized with both. Two noncarriers became carriers after treatment. The acquisition of exogenous strains after mupirocin treatment is a common phenomenon. The finding warrants the use of mupirocin only in proven carriers for decolonization purposes. Mupirocin is effective overall in decolonizing nasal carriers but less effective in decolonizing extranasal sites.

Sign in / Sign up

Export Citation Format

Share Document