scholarly journals Norovirus Is the Most Frequent Cause of Diarrhea in Hospitalized Patients in Monterrey, Mexico

Pathogens ◽  
2020 ◽  
Vol 9 (9) ◽  
pp. 672
Author(s):  
Néstor Casillas-Vega ◽  
Fernanda Flores-Rodríguez ◽  
Israel Sotelo-Coronado ◽  
Magda Elizabeth Vera-García ◽  
Aldo García-Heredia ◽  
...  
Keyword(s):  
Entamoeba Histolytica ◽  
Microscopic Observation ◽  
Hospitalized Patients ◽  
Salmonella Spp ◽  
Fecal Samples ◽  
Clostridioides Difficile ◽  
Shigella Spp ◽  
Level Hospital ◽  
Clinical Problems ◽  
Campylobacter Spp

Little information is available regarding the pathogens that cause diarrhea in hospitalized patients who also have various clinical problems. The purpose of this study was to determine the presence of pathogens in fecal samples of hospitalized patients all suffering diarrhea in addition to other problems in Mexico. Diarrheic stools from 240 patients were obtained in a third-level hospital in Monterrey, Mexico. PCR was used for the detection of Salmonella spp., Shigella spp., Campylobacter spp., Yersinia spp., Aeromonas spp., Clostridioides difficile, and norovirus GI and GII. The presence of trophozoites, cysts of protozoa, eggs, and/or helminth larvae was determined by microscopic observation. Of the 240 patients analyzed, 40.4% presented at least one of the pathogens analyzed. Norovirus was the pathogen most frequently found (28.6%), followed by bacteria (11.7%), and parasites (8.3%). The majority of co-infections were parasites + norovirus, and bacteria + norovirus. Norovirus was detected mainly in children aged 0 to 10 years (9/15, 60%). Patients aged 0–20 years did not present co-infections. Entamoeba coli and Entamoeba histolytica were the most common parasites, (8/240), and Salmonella was the most prevalent bacteria (10/240). This information can help design specific strategies useful for hospitalized people with a compromised status.

Microbiological Quality of Retail Imported Unprepared Whole Lettuces: A PHLS Food Working Group Study

1999 ◽  
Vol 62 (4) ◽  
pp. 325-328 ◽  
Author(s):  
C. LITTLE ◽  
D. ROBERTS ◽  
E. YOUNGS ◽  
J. de LOUVOIS
Keyword(s):  
Escherichia Coli ◽  
Listeria Monocytogenes ◽  
Microbiological Quality ◽  
Escherichia Coli O157 ◽  
Salmonella Spp ◽  
Shigella Spp ◽  
Natural Microflora ◽  
Production Practices ◽  
Campylobacter Spp

A study of imported unprepared whole lettuces sampled from supermarkets, greengrocers, shops, and market stalls found that all were of acceptable microbiological quality. Twenty-seven out of 151 (18%) imported lettuce samples had Enterobacteriaceae levels of 104 CFU/g or more. However, these bacteria that constitute part of the natural microflora of unprepared vegetables may also be derived from the soil and/or by poor handling. The pathogens, Salmonella spp., Shigella spp., Campylobacter spp., Escherichia coli O157:H7, Vibrio cholerae, Listeria monocytogenes, and also Escherichia coli, an indicator of fecal contamination, were not detected in any imported lettuces, indicating that hygiene, harvesting, and production practices were good. Imported lettuces with Enterobacteriaceae levels of 104 CFU/g or more varied with type of retail premises and the temperature at which the lettuces were displayed. Samples from greengrocers, shops, and market stalls were more likely to contain Enterobacteriaceae at levels in excess of 104 CFU/g than those from supermarkets.

The Incidence of Salmonella, Campylobacter, and Yersinia enterocolitica in Swine Carcasses and the Slaughterhouse Environment

1989 ◽  
Vol 52 (9) ◽  
pp. 642-645 ◽  
Author(s):  
AKIER A. MAFU ◽  
R. HIGGINS ◽  
M. NADEAU ◽  
G. COUSINEAU
Keyword(s):  
Yersinia Enterocolitica ◽  
Degree Of Contamination ◽  
Salmonella Spp ◽  
Fecal Samples ◽  
Cold Room ◽  
Tank Water ◽  
Campylobacter Spp

This study was done to evaluate the degree of contamination of cooler-ready hog carcasses and the slaughterhouse environment by Salmonella spp., Campylobacter spp. and Yersinia enterocolitica. Samples from diaphragms and feces were collected from 200 market hogs in a Quebec slaughterhouse. Scalding-tank water and environmental swabs were also collected in the slaughterhouse. Specimens were tested for the presence of Salmonella spp., Y. enterocolitica, and Campylobacter spp. Salmonella spp. were isolated from 45 (10%) of 448 samples. The distribution of the isolates were slaughtering floor (8.9%), feces (80.2%), cold-room floor (4.4%), and diaphragms (6.7%). Campylobacter spp. were isolated from 247 (61.7%) of 400 specimens, and C. coli, C. jejuni, and C. laridis accounted for 97%, 2%, and 1% of isolates, respectively. Ninety-nine percent of fecal samples were positive for the presence of C. coli. Y. enterocolitica was found in 42 (9.3%) of the 448 specimens. Of these, 85.7%, 11.9%, and 2.4% of the isolates came from fecal, diaphragm, and cold-room floor samples, respectively. Neither Salmonella spp. nor Y. enterocolitica were isolated from scalding-tank water.

scholarly journals Contamination Status of Salmonella spp., Shigella spp. and Campylobacter spp. in Surface and Groundwater of the Kelani River Basin, Sri Lanka

Water ◽  
2020 ◽  
Vol 12 (8) ◽  
pp. 2187
Author(s):  
M.G.Y.L. Mahagamage ◽  
M.V.S.C. Pathirage ◽  
Pathmalal M. Manage
Keyword(s):  
Surface Water ◽  
Sri Lanka ◽  
River Basin ◽  
Total Coliform ◽  
Salmonella Spp ◽  
E Coli ◽  
Sampling Locations ◽  
Shigella Spp ◽  
Kelani River ◽  
Campylobacter Spp

Waterborne diseases are a global problem that causes more than 2.2 million deaths annually. Therefore, the present study was focused on microbiological contamination of both ground and surface water by means of total coliform, Escherichia coli (E. coli), Salmonella spp., Shigella spp. and Campylobacter spp. Seventy two groundwater and 45 surface water sampling locations were selected to collect water from the head, transitional and meandering regions of the Kelani River Basin for a period of one year (both dry and wet seasons). The results of the study revealed that the entire Kelani River basin was contaminated with total coliform and E. coli bacteria and almost all the sampling locations exceed Sri Lanka Standards Institute (SLSI) guideline value given for drinking water (0 CFU/100 mL). Further, in groundwater, 17 locations were positive for Salmonella spp., whereas only 2 locations were positive for Campylobacter spp. In surface water, 26 and three sampling locations were positive for Salmonella spp. and Campylobacter spp., respectively. In this study, 23 different human pathogenic serovars were isolated and the Salmonella enterica serovar Kentucky was identified as the commonest type. Thus, the result of the study revealed that the consumption of raw water from the Kelani River Basin is unsafe and possible to cause gastrointestinal diseases.

scholarly journals EVALUATION OF A RAPID SCREENING ASSAY FOR BACTERIAL IDENTIFICATION (DOT-ELISA) IN FECAL SAMPLES FROM CHILDREN

1997 ◽  
Vol 39 (1) ◽  
pp. 21-28 ◽  
Author(s):  
Etelvina BOCCATTO ◽  
Sânia Alves dos SANTOS ◽  
Neusa Augusta Oliveira MAZIERI ◽  
Dahir Ramos de ANDRADE ◽  
Francisco Roque CARRAZZA ◽  
...  
Keyword(s):  
Escherichia Coli ◽  
Stool Culture ◽  
Rapid Screening ◽  
Enzyme Linked Immunosorbent Assay ◽  
Bacterial Strains ◽  
Salmonella Spp ◽  
Screening Assay ◽  
Fecal Samples ◽  
Shigella Spp ◽  
Dot Elisa

With the objective of standardizing a Dot Enzyme-Linked Immunosorbent Assay (Dot-ELISA) to detect antigens of fecal bacterial enteropathogens, 250 children, aged under 36 months and of both sexes, were studied; of which 162 had acute gastroenteritis. The efficacy of a rapid screening assay for bacterial enteropathogens (enteropathogenic Escherichia coli "EPEC", enteroinvasive Escherichia coli "EIEC", Salmonella spp. and Shigella spp.) was evaluated. The fecal samples were also submitted to a traditional method of stool culture for comparison. The concordance index between the two techniques, calculated using the Kappa (k) index for the above mentioned bacterial strains was 0.8859, 0.9055, 0.7932 and 0.7829 respectively. These values express an almost perfect degree of concordance for the first two and substantial concordance for the latter two, thus enabling this technique to be applied in the early diagnosis of diarrhea in infants. With a view to increasing the sensitivity and specificity of this immunological test, a study was made of the antigenic preparations obtained from two types of treatment: 1) deproteinization by heating; 2) precipitation and concentration of the lipopolysaccharide antigen (LPS) using an ethanol-acetone solution, which was then heated in the presence of sodium EDTA

scholarly journals Incidence and etiology of infectious diarrhea from a facility-based surveillance system in Guatemala, 2008–2012

2019 ◽  
Vol 19 (1) ◽  
Author(s):  
Wences Arvelo ◽  
Aron J. Hall ◽  
Olga Henao ◽  
Beatriz Lopez ◽  
Chris Bernart ◽  
...  
Keyword(s):  
Surveillance System ◽  
Household Survey ◽  
Salmonella Spp ◽  
Viral Pathogens ◽  
Laboratory Capacity ◽  
Diarrhea Case ◽  
Shigella Spp ◽  
And Control ◽  
Adjusted Incidence ◽  
Campylobacter Spp

Abstract Background Diarrhea is a major cause of morbidity and mortality, yet incidence and etiology data are limited. We conducted laboratory-based diarrhea surveillance in Guatemala. Methods A diarrhea case was defined as ≥3 loose stools in a 24-h period in a person presenting to the surveillance facilities. Epidemiologic data and stool specimens were collected. Specimens were tested for bacterial, parasitic, and viral pathogens. Yearly incidence was adjusted for healthcare seeking behaviors determined from a household survey conducted in the surveillance catchment area. Results From November 2008 to December 2012, the surveillance system captured 5331 diarrhea cases; among these 1381 (26%) had specimens tested for all enteric pathogens of interest. The adjusted incidence averaged 659 diarrhea cases per 10,000 persons per year, and was highest among children aged < 5 years, averaging 1584 cases per 10,000 children per year. Among 1381 (26%) specimens tested for all the pathogens of interest, 235 (17%) had a viral etiology, 275 (20%) had a bacterial, 50 (4%) had parasites, and 86 (6%) had co-infections. Among 827 (60%) specimens from children aged < 5 years, a virus was identified in 196 (23%) patients; 165 (20%) had norovirus and 99 (12%) rotavirus, including co-infections. Among 554 patients aged ≥5 years, 103 (19%) had a bacterial etiology, including diarrheagenic Escherichia coli in 94 (17%) cases, Shigella spp. in 31 (6%), Campylobacter spp. in 5 (1%), and Salmonella spp. in 4 (1%) cases. Detection of Giardia and Cryptosporidium was infrequent (73 cases; 5%). Conclusions There was a substantial burden of viral and bacterial diarrheal diseases in Guatemala, highlighting the importance of strengthening laboratory capacity for rapid detection and control and for evaluation of public health interventions.

scholarly journals Antibiotic sensitivity pattern of diarrhoeal pathogens in under five children

2020 ◽  
Vol 52 (1-2) ◽  
pp. 35-39
Author(s):  
Mst Khashruba Pervin ◽  
Sanya Tahmina Jhora ◽  
Shikha Paul ◽  
Akhtarun Naher
Keyword(s):  
Escherichia Coli ◽  
Membrane Filter ◽  
Enteric Bacteria ◽  
Salmonella Spp ◽  
Medical College ◽  
Antimicrobial Sensitivity ◽  
Sensitivity Pattern ◽  
Shigella Spp ◽  
Agar Media ◽  
Campylobacter Spp

Background: Different bacterial enteropathogens are responsible for diarrhoea in children less than 5 years of age in Bangladesh. This study aimed to determine the antimicrobial sensitivity pattern of diarrhoeal pathogens that is necessary to know for the specific management of acute bacterial diarrhoea. Objectives: To find out the bacterial enteropathogens causing diarrhoea and their antimicrobial sensitivity pattern in under 5 children. Methodology: This was an observational cross sectional study carried out at Microbiology Department of Sir Salimullah Medical College, Dhaka. A total of 217 diarrhoeal patients 0-5 years age, attending Pediatric unit of Sir Salimullah Medical College and Mitford Hospital. Dhaka and Dhaka Shishu Hospital, Dhaka during the period of July, 2015 to June, 2016, were enrolled in the study. For isolation of the common enteric bacteria, diarrhoeal stools were cultured on MacConkey agar media and for Campylobacter spp, diarrhoeal stools were cultured on selective Modified Campy BAP media and nonselective Blood agar media with membrane filter. Positive culture was confirmed by Gram stain morphology and standard biochemical tests. Antimicrobial susceptibility test were used to identify the bacterial sensitivity pattern in the isolates. Results: Out of 217 diarrhoeal stools, bacteria was isolated from 97 (44.70%) patients by culture. Among 97 culture positive cases Eschericha coli (51- 52.58%), Campylobacter spp. (23-23.71%), Shigella spp. (15-15.46%) and Salmonella spp. (8-8.25%) were isolated. Mecillinam is the most sensitive drug for Escherichia coli, Campylobacter spp., Shigella spp. and Salmonella spp. Among the other antimicrobials, Campylabacter spp. were sensitive to azithromycin, erythromycin and nitrofurantoin. Escherichia coli were sensitive to nitrofurantoin, gentamicin and azithromycin. Shigella spp. were sensitive to ceftriaxone, ciprofloxacin and nitrofurantoin and Salmonella spp. were sensitive to ceftriaxone, cotrimoxazole and gentamicin. Conclusion: Mecillinam and nitrofurantoin were highly effective against bacteria causing diarrhoea in children. Bang Med J (Khulna) 2019; 52 : 35-39

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