scholarly journals Antibacterial Drug-Release Polydimethylsiloxane Coating for 3D-Printing Dental Polymer: Surface Alterations and Antimicrobial Effects

2020 ◽  
Vol 13 (10) ◽  
pp. 304
Author(s):  
Hang-Nga Mai ◽  
Dong Choon Hyun ◽  
Ju Hayng Park ◽  
Do-Yeon Kim ◽  
Sang Min Lee ◽  
...  
Keyword(s):  
Antibacterial Activity ◽  
3D Printing ◽  
Culture Media ◽  
Mesoporous Silica Nanoparticles ◽  
Polymer Surface ◽  
Surface Irregularity ◽  
Antibacterial Drug ◽  
High Porosity ◽  
Coating Film ◽  
Bacterial Colony

Polymers are the most commonly used material for three-dimensional (3D) printing in dentistry; however, the high porosity and water absorptiveness of the material adversely influence biofilm formation on the surface of the 3D-printed dental prostheses. This study evaluated the effects of a newly developed chlorhexidine (CHX)-loaded polydimethylsiloxane (PDMS)-based coating material on the surface microstructure, surface wettability and antibacterial activity of 3D-printing dental polymer. First, mesoporous silica nanoparticles (MSN) were used to encapsulate CHX, and the combination was added to PDMS to synthesize the antibacterial agent-releasing coating substance. Then, a thin coating film was formed on the 3D-printing polymer specimens using oxygen plasma and thermal treatment. The results show that using the coating substance significantly reduced the surface irregularity and increased the hydrophobicity of the specimens. Remarkably, the culture media containing coated specimens had a significantly lower number of bacterial colony formation units than the noncoated specimens, thereby indicating the effective antibacterial activity of the coating.

scholarly journals Study of antibacterial activities of the volatile oil of Nigella sativa Linn on animal model

1970 ◽  
pp. 22-24
Author(s):  
Nazma Ara ◽  
MA Khaleque ◽  
SAR Choudhury ◽  
Ruhul Amin
Keyword(s):  
Animal Model ◽  
Antibacterial Activity ◽  
Nigella Sativa ◽  
Volatile Oil ◽  
Antibacterial Activities ◽  
Bacterial Colony ◽  
Staph Aureus

In-vivo antibacterial activity of the volatile oil of Nigella sativa linn was carried out in mice. Mice were infected by inoculation of staph. Aureus and result shows successful iradication of bacterial colony. DOI: 10.3329/bjpp.v22i1.3565 Bangladesh J Physiol Pharmacol 2006; 22(1/2) : 22-24

Porous polymeric materials by 3D printing of photocurable resin

2017 ◽  
Vol 4 (3) ◽  
pp. 442-449 ◽  
Author(s):  
X. Mu ◽  
T. Bertron ◽  
C. Dunn ◽  
H. Qiao ◽  
J. Wu ◽  
...  
Keyword(s):  
3D Printing ◽  
Polymeric Materials ◽  
High Porosity

A method for 3D printing of complicated structures using a photopolymer with high porosity was developed.

Aerogels as promising materials for antibacterial applications: a mini-review

2021 ◽  
Author(s):  
Gulcihan Guzel Kaya ◽  
Elena Aznar ◽  
Huseyin Deveci ◽  
Ramón Martínez-Máñez
Keyword(s):  
Antibacterial Activity ◽  
Low Density ◽  
High Porosity

Aerogels with ultra-low density, high porosity, tunable sizes, and biocompatibility have been regarded as promising carriers for antibacterial applications. Different approaches can be followed to obtain such beneficial antibacterial activity.

scholarly journals Microbial and proximate composition of fresh and salted Hilsa, Tenualosa ilisha

2015 ◽  
Vol 42 (2) ◽  
pp. 227-236
Author(s):  
Tahara Rohomania ◽  
Mihir Lal Saha ◽  
Anwar Hossain ◽  
Shankar Chandra Mandal ◽  
Mohammad Shamsur Rahman
Keyword(s):  
Proximate Composition ◽  
Culture Media ◽  
Agar Plate ◽  
Bacterial Count ◽  
Ventral Part ◽  
Fish Sample ◽  
Bacterial Colony ◽  
Fresh Fish ◽  
Salted Fish ◽  
Fish Samples

Bacterial and nutritional quality of fresh and salted hilsa collected from four markets of Dhaka city were investigated. Five different culture media viz. nutrient agar, EMB agar for coliform, SS agar for Salmonella-Shigella, MSA agar for Staphylococcus and TCBS for Vibrio were used. The highest heterotrophic bacterial count 1.22 ± 0.12 × 106 cfu/g was recorded in the fresh fish sample of Karwan Bazar. Maximum coliform count, 1.20 ± 0.10 × 106 cfu/g was detected in the fresh fish sample of the same market. No bacterial colony was found on SS agar and TCBS agar plate in salted fish. Proximate composition of raw hilsa of dorsal and ventral part was 56.49 ± 0.13% and 55.45 ± 0.06% moisture, 23.62 ± 0.28% and 22.99 ± 0.36% protein, 18.01 ± 0.39% and 18.96 ± 0.43% fat and 1.71 ± 0.04% and 2.26 ± 0.09% ash, respectively. In salted T. ilisha, the proximate composition of dorsal and ventral part was 45.13 ± 0.54% and 40.20±0.20% moisture, 20.79 ± 0.17% and 21.48 ± 0.15% protein, 16.89 ± 0.47% and 19.54 ± 0.26% lipid and 16.65 ± 0.41% and 18.35 ± 0.08% ash. The fresh fish samples were associated with high bacterial loads than that of salted fish. The protein, lipid, moisture contents decreased and ash content increased after salting condition.Bangladesh J. Zool. 42(2): 227-236, 2014

scholarly journals Rapid Fabrication of Anatomically-Shaped Bone Scaffolds Using Indirect 3D Printing and Perfusion Techniques

2020 ◽  
Vol 21 (1) ◽  
pp. 315 ◽  
Author(s):  
Brian E. Grottkau ◽  
Zhixin Hui ◽  
Yang Yao ◽  
Yonggang Pang
Keyword(s):  
3D Printing ◽  
Time Course ◽  
Porous Scaffold ◽  
High Surface ◽  
Water Soluble ◽  
Calcium Deposition ◽  
Patient Specific ◽  
Residual Water ◽  
High Porosity ◽  
Surface Detail

Fused deposit modeling (FDM) 3D printing technology cannot generate scaffolds with high porosity while maintaining good integrity, anatomical-surface detail, or high surface area-to-volume ratio (S/V). Solvent casting and particulate leaching (SCPL) technique generates scaffolds with high porosity and high S/V. However, it is challenging to generate complex-shaped scaffolds; and solvent, particle and residual water removal are time consuming. Here we report techniques surmounting these problems, successfully generating a highly porous scaffold with the anatomical-shape characteristics of a human femur by polylactic acid polymer (PLA) and PLA-hydroxyapatite (HA) casting and salt leaching. The mold is water soluble and is easily removable. By perfusing with ethanol, water, and dry air sequentially, the solvent, salt, and residual water were removed 20 fold faster than utilizing conventional methods. The porosities are uniform throughout the femoral shaped scaffold generated with PLA or PLA-HA. Both scaffolds demonstrated good biocompatibility with the pre-osteoblasts (MC3T3-E1) fully attaching to the scaffold within 8 h. The cells demonstrated high viability and proliferation throughout the entire time course. The HA-incorporated scaffolds demonstrated significantly higher compressive strength, modulus and osteoinductivity as evidenced by higher levels of alkaline-phosphatase activity and calcium deposition. When 3D printing a 3D model at 95% porosity or above, our technology preserves integrity and surface detail when compared with FDM-generated scaffolds. Our technology can also generate scaffolds with a 31 fold larger S/V than FDM. We have developed a technology that is a versatile tool in creating personalized, patient-specific bone graft scaffolds efficiently with high porosity, good scaffold integrity, high anatomical-shaped surface detail and large S/V.

scholarly journals Genomic and Metabolomic Analysis of Antarctic Bacteria Revealed Culture and Elicitation Conditions for the Production of Antimicrobial Compounds

Biomolecules ◽  
2020 ◽  
Vol 10 (5) ◽  
pp. 673
Author(s):  
Kattia Núñez-Montero ◽  
Damián Quezada-Solís ◽  
Zeinab G. Khalil ◽  
Robert J. Capon ◽  
Fernando D. Andreote ◽  
...  
Keyword(s):  
Antibacterial Activity ◽  
Secondary Metabolites ◽  
Culture Media ◽  
Genome Mining ◽  
Gene Clusters ◽  
Culture Conditions ◽  
Bioactive Metabolites ◽  
Bacterial Strains ◽  
Antarctic Bacteria ◽  
New Antibiotics

Concern about finding new antibiotics against drug-resistant pathogens is increasing every year. Antarctic bacteria have been proposed as an unexplored source of bioactive metabolites; however, most biosynthetic gene clusters (BGCs) producing secondary metabolites remain silent under common culture conditions. Our work aimed to characterize elicitation conditions for the production of antibacterial secondary metabolites from 34 Antarctic bacterial strains based on MS/MS metabolomics and genome mining approaches. Bacterial strains were cultivated under different nutrient and elicitation conditions, including the addition of lipopolysaccharide (LPS), sodium nitroprusside (SNP), and coculture. Metabolomes were obtained by HPLC-QTOF-MS/MS and analyzed through molecular networking. Antibacterial activity was determined, and seven strains were selected for genome sequencing and analysis. Biosynthesis pathways were activated by all the elicitation treatments, which varies among strains and dependents of culture media. Increased antibacterial activity was observed for a few strains and addition of LPS was related with inhibition of Gram-negative pathogens. Antibiotic BGCs were found for all selected strains and the expressions of putative actinomycin, carotenoids, and bacillibactin were characterized by comparison of genomic and metabolomic data. This work established the use of promising new elicitors for bioprospection of Antarctic bacteria and highlights the importance of new “-omics” comparative approaches for drug discovery.

scholarly journals An Evaluation of the Antibacterial Properties of Tormentic Acid Congener and Extracts From Callistemon viminalis on Selected ESKAPE Pathogens and Effects on Biofilm Formation

2020 ◽  
Vol 2020 ◽  
pp. 1-14
Author(s):  
Tafadzwa Chipenzi ◽  
Genuine Baloyi ◽  
Tatenda Mudondo ◽  
Simbarashe Sithole ◽  
Godloves Fru Chi ◽  
...  
Keyword(s):  
Antibacterial Activity ◽  
Biofilm Formation ◽  
Antibacterial Properties ◽  
Extracellular Dna ◽  
Dilution Method ◽  
Antibacterial Drug ◽  
Antibacterial Effects ◽  
Callistemon Viminalis ◽  
Eskape Pathogens ◽  
Tormentic Acid

ESKAPE pathogens, namely, Enterococcus faecium, Staphylococcus aureus, Klebsiella pneumoniae, Acinetobacter baumannii, Pseudomonas aeruginosa, and Enterobacter species, are responsible for a majority of all healthcare-acquired infections (HAI). The bacteria cause nosocomial infections in immunocompromised patients. Extracts from Callistemon viminalis have been shown to have antibacterial, antifungal, and anti-inflammatory activities. Tormentic acid congener, a pentacyclic triterpene saponin, was isolated from C. viminalis leaves. This study aimed to investigate the antibacterial effects of tormentic acid congener and leaf extracts on biofilm formation by A. baumannii, S. aureus, S. pyogenes, and P. aeruginosa. The antibacterial effects were determined by the microbroth dilution method, and ciprofloxacin was used as the standard antibacterial drug. Biofilm formation and detachment assays were performed using crystal violet staining. Production of extracellular polymeric DNA and polysaccharides from biofilms was also determined. Tormentic acid congener showed time-dependent antibacterial activity against P. aeruginosa with a MIC of 100 µg/ml and caused significant protein leakage. Antibacterial activity was found when tormentic acid congener was tested against both S. aureus and P. aeruginosa. The MICs were found to be 25 µg/ml and 12.5 µg/ml for P. aeruginosa and S. aureus cells, respectively. S. pyogenes was found to be susceptible to tormentic acid congener and the hydroethanolic extract with an MIC of 100 µg/ml and 25 µg/ml, respectively. A. baumannii was found not to be susceptible to the compound or the extracts. The compound and the extracts caused a significant decrease in the biofilm extracellular polysaccharide content of S. pyogenes. The extracts and tormentic acid congener caused detachment of biofilms and decreased the release of extracellular DNA and capsular polysaccharides from biofilms of P. aeruginosa and S. aureus. Tormentic acid congener and extracts, thus, have significant antibacterial and antibiofilm activities on these selected ESKAPE bacteria and can act as source lead compounds for the development of antibacterial triterpenoids.

scholarly journals SYNTHESIS AND CHARACTERIZATION OF NOVEL SA-PA-LSA/C-30B/AG NANOCOMPOSITES FOR SWELLING, ANTIBACTERIAL, DRUG DELIVERY, AND ANTICANCER APPLICATIONS

2018 ◽  
Vol 11 (3) ◽  
pp. 229
Author(s):  
Nanjunda Reddy B H ◽  
Prdadipta Ranjan Rauta ◽  
Venkatalakshimi V ◽  
Swamy Sreenivasa
Keyword(s):  
Drug Delivery ◽  
Antibacterial Activity ◽  
Sustained Release ◽  
Polymerization Reaction ◽  
Antibacterial Drug ◽  
Hacat Cells ◽  
Swelling Properties ◽  
Hydrogel Matrix ◽  
Hydrogel Composites ◽  
Lignosulfonic Acid

 Objective: The main objective of this work was to formulate and evaluate Closite-30B/nanoAg filled hydrogel composites which are further intentended to be used for the study of drug delivery,antibacterial, and anticancer activityMethods: In this study, Cloisite-30B (C-30B) clay dispersed biopolymer sodium alginate (SA)-grafted-poly (acrylamide [AAm]-co-lignosulfonic acid) hydrogel composites were synthesized by free radical in situ polymerization reaction technique using SA, AAm, and lignosulfonic acid biopolymers in different proportions in combination. which are subjected to invitro drug delivery and Minimum inhibitory concentration(MIC) method for antibacterial activity study by using Streptococcus faecalis (S.faecalis) and Escherichia coli (E. coli)bacteria. The biocompatibility of the prepared gels were determined by standard protocol HaCaT-cells and MCF-7 cell lines further the prepared hydrogel composites were characterized for particle size,encapsulation efficiency,swelling properties,compatibility studies by FTIR etc.Results: The formulated hydrogels were characterized by X-ray diffraction (XRD) to analyze the particles size and crystallinity. The presence of functional groups and their chemical interaction with the drug, C-30B, and silver nanoparticles (AgNPs) were confirmed by the FTIR spectroscopy. Furthermore, the presence of AgNPs in the matrix was confirmed by ultraviolet/visible spectroscopy. Thermogravimetric analysis was performed to find out the thermal degradation, thermal stability, and the percentage of weight loss at various temperatures. Swelling studies revealed that C-30B and AgNPs induced composites exhibited higher swelling ratio than pure hydrogels. The hydrogels with C-30B/AgNPs displayed excellent antibacterial activity against both Gram-positive and Gram-negative bacteria. Further, these hydrogel composites were loaded with the drug paclitaxel (PT), and drug release study showed that the sustained release of the drug from C-30B/Ag hydrogel matrix compared to rest of other samples. Hydrogel composites were cytocompatible in nature (with HaCaT cells) and the cell viability decreased (with MCF-7cells) with the presence of lignosulfonic acid as well as C-30B and AgNPs in the samples as evaluated through 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyl tetrazolium bromide to its insoluble formazan assay.Conclusion: The synthesized hydrogel composites were successfully characterized and eavaluated for sustained release of paclitaxel drug delivery at different pHs and temperatures and it is found that C30B/Ag filled composites exhibits contolled release of drug with higher rate, especially at lower pH (pH2) and higher temperature (37oC) and the same formulations which exhibits better anitbcterial and anticancer activity compared to the virgin samples So the prepared C30B/AgNPs hydrogels composites used in drug dlivery for the effective treatment of cancer and used against bacterias and cancerous cells.

Deposition of Hard Coating Film on Polymer Surface by Plasma CVD

2002 ◽  
Author(s):  
Hidetaka Anma ◽  
Yuuji Yoshimoto ◽  
Mariko Tanaka ◽  
Hiroyuki Takatsuka ◽  
Yoshinori Hatanaka
Keyword(s):  
Polymer Surface ◽  
Hard Coating ◽  
Coating Film ◽  
Plasma Cvd
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