scholarly journals Polymeric Nanoparticles of Pistacia lentiscus var. chia Essential Oil for Cutaneous Applications

Pharmaceutics ◽  
2020 ◽  
Vol 12 (4) ◽  
pp. 353
Author(s):  
Ilianna Vrouvaki ◽  
Eleni Koutra ◽  
Michael Kornaros ◽  
Konstantinos Avgoustakis ◽  
Fotini N. Lamari ◽  
...  
Keyword(s):  
Essential Oil ◽  
Polymeric Nanoparticles ◽  
In Vitro Release ◽  
Physicochemical Characteristics ◽  
Poly Lactic Acid ◽  
Poly Vinyl Alcohol ◽  
Pistacia Lentiscus ◽  
Shell Material ◽  
Sem Images

Polymeric nanoparticles (NPs) encapsulating Pistacia lentiscus L. var. chia essential oil (EO) were prepared by a solvent evaporation method, in order to obtain a novel carrier for administration on the skin. The specific EO exhibits antimicrobial and anti-inflammatory properties thus stimulating considerable interest as a novel agent for the treatment of minor skin inflammations. The incorporation into nanoparticles could overcome the administration limitations that inserts the nature of the EO. Nanoparticles were prepared, utilizing poly(lactic acid) (PLA) as shell material, due to its biocompatibility and biodegradability, while the influence of surfactant type on NPs properties was examined. Two surfactants were selected, namely poly(vinyl alcohol) (PVA) and lecithin (LEC) and NPs’ physicochemical characteristics i.e. size, polydispersity index (PdI) and ζ-potential were determined, not indicating significant differences (p > 0.05) between PLA/PVA-NPs (239.9 nm, 0.081, -29.1 mV) and PLA/LEC-NPs (286.1 nm, 0.167, −34.5 mV). However, encapsulation efficiency (%EE) measured by GC-MS, was clearly higher for PLA/PVA-NPs than PLA/LEC-NPs (37.45% vs. 9.15%, respectively). Moreover PLA/PVA-NPs remained stable over a period of 60 days. The in vitro release study indicated gradual release of the EO from PLA/PVA-NPs and more immediate from PLA/LEC-NPs. The above findings, in addition to the SEM images of the particles propose a potential structure of nanocapsules for PLA/PVA-NPs, where shell material is mainly consisted of PLA, enclosing the EO in the core. However, this does not seem to be the case for PLA/LEC-NPs, as the results indicated low EO content, rapid release and a considerable percentage of humidity detected by SEM. Furthermore, the Minimum Inhibitory Concentration (MIC) of the EO was determined against Escherichia coli and Bacillus subtilis, while NPs, however did not exhibit considerable activity in the concentration range applied. In conclusion, the surfactant selection may modify the release of EO incorporated in NPs for topical application allowing its action without interfering to the physiological skin microbiota.

scholarly journals Fabrication, Characterization, In Vitro Release, and Some Biological Activities of Eucalyptus Essential Oil Loaded Poly (Lactic Acid) Nanofibers

2021 ◽  
Vol 4 (2) ◽  
pp. 54-63
Author(s):  
Hayfa Argui ◽  
Salih Can Suner ◽  
Çağdaş Deniz Periz ◽  
Seyhan Ulusoy ◽  
Mossadok Ben-Attia ◽  
...  
Keyword(s):  
Lactic Acid ◽  
Essential Oil ◽  
Biological Activities ◽  
In Vitro Release ◽  
Poly Lactic Acid ◽  
Vitro Release ◽  
Eucalyptus Essential Oil

Design of Leather Footwear for Diabetics Containing Chlohexidine Digluconate Microparticles

2020 ◽  
Vol 115 (03) ◽  
pp. 79-86
Author(s):  
Fatih Yalcin ◽  
Huseyin Ata Karavana ◽  
Seda Rencber ◽  
Sinem Yaprak Karavana
Keyword(s):  
In Vitro Release ◽  
Shell Material ◽  
Sem Images ◽  
Functional Quality ◽  
Release Studies ◽  
Vitro Release ◽  
Spray Dried ◽  
Scanning Electron

ChlorHexidine Digluconate [CHD] was encapsulated inside an ethylcellulose shell material [Aquacoat ECD], and then spray dried to produce mixed microparticles (MPs). The validity and functional quality of the resultant [CHD-MPs] were analyzed on vamp and lining leather which are used to manufacture shoes for diabetics. The morphology, efficiency of encapsulation and in vitro release characteristics of the [CHD-MPs] were optimized in order to impregnate [CHD-MPs] onto leather footwear for diabetics. Scanning electron microscopy (SEM) was used to characterize the [CHD-MPs] and the leathers treated with it. SEM images illustrated that the [CHD-MPs] were spherical, smooth in shape and adhered well to leather. In vitro CHD-release studies from its MPs, and for leather treated with it were performed in phosphate buffer saline at pH =7.2. There was an inherently controlled release behaviour of CHD for all the formulations on leather. Finally, microbiologic studies on leather treated with [CHD-MPs] were done. This study suggested that footwear containing [CHD- MPs] is/will improve the quality of daily life for diabetics. 

scholarly journals Development of Nitazoxanide Loaded Polymeric Nanocarriers: Box Behnken Experimental Design Based Optimisation and Characterisation

2020 ◽  
pp. 34-53
Author(s):  
Charu Bharti ◽  
Shrestha Sharma ◽  
Shobhit Kumar ◽  
Syed Arman Rabbani
Keyword(s):  
Polymeric Nanoparticles ◽  
Polymer Concentration ◽  
In Vitro Release ◽  
Entrapment Efficiency ◽  
Poly Vinyl Alcohol ◽  
Cross Linking ◽  
Surface Geometry ◽  
Sustained Drug Release ◽  
Model Drug

The current investigation is focused on formulation, optimisation and characterisation of polymeric based nanomaterial. Nitazoxanide (NTZ) loaded polymeric nanoparticles were prepared by homogenisation technique using Eudragit RL100 as a polymer matrix and Poly vinyl alcohol (PVA) as a cross linking agent. NTZ was used as a model drug and investigated for preformulation parameters along with excipients, identification of concentration for optimization, selection of independent (X) and dependent (Y) variables and characterisation of optimised formulation. Polymeric nanoparticles were obtained after optimization using 33 factorial design by Box Behnken Design expert (BBD). The role and influence of key process variables i.e. concentration of polymer, concentration of cross linking agent and speed of rotation of homogeniser at their respective three different levels for the optimisation of formulation were also investigated. The synthesised optimised polymeric nanoparticles were further characterised by dynamic light scattering (DLS) for its particle size (137.11nm), PDI (0.180) and zeta potential (33.4 mV) while X-ray diffraction (XRD) was used to justify the amorphous and crystalline nature of drug and excipients. Transmission electron microscopy (TEM) further revealed surface geometry of these nanoparticles being spherical in shape, drug entrapment efficiency (%DEE) was found to be 81.89% and in vitro release studies showed sustained drug release effect. The antimicrobial activity against Pseudomonas aeruginosa, Streptococcus mutans and Escherichia coli was also determined.

scholarly journals RESVERATROL-HUMIC ACID LOADED COLLOIDAL POLYMERIC NANOPARTICLES: DESIGN OF EXPERIMENT AND IN VITRO EVALUATION

2021 ◽  
pp. 177-184
Author(s):  
RAHUL H. ◽  
SUNDEEP C. ◽  
SWATI G.
Keyword(s):  
Humic Acid ◽  
Polymeric Nanoparticles ◽  
In Vitro Release ◽  
Physicochemical Characteristics ◽  
Polymeric Nanocarriers ◽  
Eudragit E100 ◽  
Formulation Factors ◽  
The Stability ◽  
Vitro Release

Objective: The study aims at fabrication and optimization of co-encapsulated resveratrol and humic acid in colloidal polymeric nanocarriers to improves the stability of insoluble antioxidant, resveratrol. Methods: The Eudragit E100 polymeric material was used to fabricate oral co-encapsulation of resveratrol and humic acid in colloidal polymeric nanocarriers, called Res-HA-co-CPNs by an emulsification-diffusion-evaporation method. Taguchi orthogonal array design was employed to check the effect of formulation factors on in vitro physicochemical characteristics. The optimized formulation was further evaluated for stability studies at different conditions. Results: Optimized Res-HA-co-CPNs demonstrated spherical and smooth surface including mean PS 120.56±18.8 nm, PDI 0.122, ZP+38.25mV, and EE 82.37±1.49%. Solid-state characterizations confirmed that optimized Res-HA-co-CPNs showed amorphous characteristics. In vitro release profile of Res-HA-co-CPNs showed an outstanding sustained release behavior up to 48h and remain stable at the refrigerated condition for 6 mo. Conclusion: Res-HA-co-CPNs would be a proficient and promising dosage form for increasing the stability of insoluble antioxidants, resveratrol.

scholarly journals Cefotaxime Loaded Polycaprolactone Based Polymeric Nanoparticles with Antifouling Properties for In-Vitro Drug Release Applications

Polymers ◽  
2021 ◽  
Vol 13 (13) ◽  
pp. 2180
Author(s):  
Sana Javaid ◽  
Nasir M. Ahmad ◽  
Azhar Mahmood ◽  
Habib Nasir ◽  
Mudassir Iqbal ◽  
...  
Keyword(s):  
Drug Release ◽  
Polymeric Nanoparticles ◽  
In Vitro Release ◽  
Bacterial Strains ◽  
In Vitro Drug Release ◽  
Antifouling Activity ◽  
Polymeric Drug ◽  
Pure Drug ◽  
Average Size

The objective of the present study was to achieve the successful encapsulation of a therapeutic agent to achieve antifouling functionality regarding biomedical applications. Considering nanotechnology, drug-loaded polycaprolactone (PCL)-based nanoparticles were prepared using a nano-precipitation technique by optimizing various process parameters. The resultant nano-formulations were investigated for in vitro drug release and antifouling applications. The prepared particles were characterized in terms of surface morphology and surface properties. Optimized blank and drug-loaded nanoparticles had an average size of 200 nm and 216 nm, respectively, with associated charges of −16.8 mV and −11.2 mV. Studies of the in vitro release of drug were carried out, which showed sustained release at two different pH, 5.5 and 7.4 Antifouling activity was observed against two bacterial strains, Gram-positive Staphylococcus aureus and Gram-negative Escherichia coli. The zone of inhibition of the optimized polymeric drug-loaded nanoparticle F-25 against both strains were compared with the pure drug. The gradual pH-responsive release of antibiotics from the biodegradable polymeric nanoparticles could significantly increase the efficiency and pharmacokinetics of the drug as compared to the pure drug. The acquired data significantly noted that the resultant nano-encapsulation of antifouling functionality could be a promising candidate for topical drug delivery systems and skin applications.

scholarly journals Encapsulation in Polymeric Nanoparticles Enhances the Enzymatic Stability and the Permeability of the GLP-1 Analog, Liraglutide, Across a Culture Model of Intestinal Permeability

Pharmaceutics ◽  
2019 ◽  
Vol 11 (11) ◽  
pp. 599 ◽  
Author(s):  
Ruba Ismail ◽  
Alexandra Bocsik ◽  
Gábor Katona ◽  
Ilona Gróf ◽  
Mária A. Deli ◽  
...  
Keyword(s):  
Cell Model ◽  
Morphological Changes ◽  
Polymeric Nanoparticles ◽  
Release Kinetics ◽  
In Vitro Release ◽  
Intestinal Barrier ◽  
Double Emulsion ◽  
Intercellular Junctions ◽  
Enzyme Degradation

The potential of poly (lactic-co-glycolic acid) nanoparticles (PLGA NPs) to overcome the intestinal barrier that limits oral liraglutide delivery was evaluated. Liraglutide-loaded PLGA NPs were prepared by the double emulsion solvent evaporation method. In vitro release kinetics and enzymatic degradation studies were conducted, mimicking the gastrointestinal environment. The permeability of liraglutide solution, liraglutide-loaded PLGA NPs, and liraglutide in the presence of the absorption enhancer PN159 peptide was tested on the Caco-2 cell model. Liraglutide release from PLGA NPs showed a biphasic release pattern with a burst effect of less than 15%. The PLGA nanosystem protected the encapsulated liraglutide from the conditions simulating the gastric environment. The permeability of liraglutide encapsulated in PLGA NPs was 1.5-fold higher (24 × 10−6 cm/s) across Caco-2 cells as compared to liraglutide solution. PLGA NPs were as effective at elevating liraglutide penetration as the tight junction-opening PN159 peptide. No morphological changes were seen in the intercellular junctions of Caco-2 cells after treatment with liraglutide-PLGA NPs, confirming the lack of a paracellular component in the transport mechanism. PLGA NPs, by protecting liraglutide from enzyme degradation and enhancing its permeability across intestinal epithelium, hold great potential as carriers for oral GLP-1 analog delivery.

Evaluation of the in vitro release and permeation of Cordia verbenacea DC essential oil from topical dosage forms

2019 ◽  
Vol 53 ◽  
pp. 101173 ◽  
Author(s):  
Jéssica Domingos da Silva ◽  
Márcio Vinícius Gomes ◽  
Lucio Mendes Cabral ◽  
Valeria Pereira de Sousa
Keyword(s):  
Essential Oil ◽  
In Vitro Release ◽  
Dosage Forms ◽  
Topical Dosage ◽  
Vitro Release ◽  
Topical Dosage Forms
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