scholarly journals Modification of Proliferation and Apoptosis in Breast Cancer Cells by Exposure of Antioxidant Nanoparticles Due to Modulation of the Cellular Redox State Induced by Doxorubicin Exposure

Pharmaceutics ◽  
2021 ◽  
Vol 13 (8) ◽  
pp. 1251
Author(s):  
Laura Denise López-Barrera ◽  
Roberto Díaz-Torres ◽  
Joselo Ramón Martínez-Rosas ◽  
Ana María Salazar ◽  
Carlos Rosales ◽  
...  

In this report, we investigated whether the use of chitosan-carrying-glutathione nanoparticles (CH-GSH NPs) can modify proliferation and apoptosis, and reduce cell damage induced by doxorubicin on breast cancer cells. Doxorubicin is a widely used antineoplasic agent for the treatment of various types of cancer. However, it is also a highly toxic drug because it induces oxidative stress. Thus, the use of antioxidant molecules has been considered to reduce the toxicity of doxorubicin. CH-GSH NPs were characterized in size, zeta potential, concentration, and shape. When breast cancer cells were treated with CH-GSH nanoparticles, they were localized in the cellular cytoplasm. Combined doxorubicin exposure with nanoparticles increased intracellular GSH levels. At the same time, decreasing levels of reactive oxygen species and malondialdehyde were observed and modified antioxidant enzyme activity. Levels of the Ki67 protein were evaluated as a marker of cell proliferation and the activity of the Casp-3 protein related to cell apoptosis was measured. Our data suggests that CH-GSH NPs can modify cell proliferation by decreasing Ki67 levels, induce apoptosis by increasing caspase-3 activity, and reduce the oxidative stress induced by doxorubicin in breast cancer cells by modulating molecules associated with the cellular redox state. CH-GSH NPs could be used to reduce the toxic effects of this antineoplastic. Considering these results, CH-GSH NPs represent a novel delivery system offering new opportunities in pharmacy, material science, and biomedicine.

PLoS ONE ◽  
2013 ◽  
Vol 8 (6) ◽  
pp. e67191 ◽  
Author(s):  
Aejaz Sayeed ◽  
Gloria Luciani-Torres ◽  
Zhenhang Meng ◽  
James L. Bennington ◽  
Dan H. Moore ◽  
...  

2019 ◽  
Vol 7 (19) ◽  
pp. 3169-3176
Author(s):  
Hussain Al Ssadh ◽  
Waleed Al Abdulmonem ◽  
Zafar Rasheed ◽  
Inamul Hasan Madar ◽  
Jamila Alhoderi ◽  
...  

BACKGROUND: The cluster of differentiation (CD) 74 is known for its immunological functions and its elevated level was reported in various cancer cells. AIM: The aim of the present study was to investigate the expression and potential roles of CD74 in the proliferative and apoptotic activity of breast cancer. METHODS: Expression of CD74, macrophage migration inhibitory factor (MIF) and CD44 was assayed in CAMA-1 and MDA-MB-231 cell lines using flow cytometry. CD74 was knocked down using CD74 siRNA-transfection in CAMA-1, and MDA-MB-231 cells and proliferation and apoptosis were determined in the transfected breast cancer cells. RESULTS: The data showed that CD74, MIF and CD44 were expressed in breast cancer cell lines and were associated with cell proliferation and apoptosis. Correlation analysis revealed that CD74 was positively correlated and colocalised with MIF on the cell-surface of CAMA-1 and MDA-MB-231. The knockdown of CD74 significantly reduced CAMA-1 and MDA-MB-231 cell proliferation and increased the level of apoptotic cells. CONCLUSION: We concluded that the interactions of CD74 with MIF and CD74 with CD44 could be a potential tumour marker for breast cancer cells. Moreover, the level of co-expression of MIF and CD74 or CD44 could be a surrogate marker for the efficacy of anti-angiogenic drugs, particularly in breast cancer tumours. In short, the study revealed the potential roles of CD74 in the proliferation and apoptosis of breast cancer which may serve as a potential therapeutic target for breast cancer.


2020 ◽  
Author(s):  
Laura D López-Barrera ◽  
Roberto Díaz-Torres ◽  
Joselo R Martínez-Rosas ◽  
Ana M Salazar Martínez ◽  
Carlos Rosales ◽  
...  

Doxorubicin is a widely used antineoplastic agent for the treatment of various types of cancer. However, it is also a highly toxic drug because it induces the generation of oxidative stress. Thus, the use of antioxidant molecules has been considered to reduce the toxicity of doxorubicin. In this report, we investigated whether the use of chitosan-glutathione (CH-GSH) nanoparticles could reduce cell damage induced by doxorubicin on breast cancer cells. CH-GSH NPs were characterized in size, Zeta potential, concentration, and shape. When breast cancer cells were treated with CH-GSH nanoparticles, these were localized in the cellular cytoplasm. Combined exposure of doxorubicin and nanoparticle increased intracellular GSH levels while decreasing reactive oxygen species and malondialdehyde levels. The antioxidant enzyme activity was also decreased. Together our data suggest that the use of CH-GSH nanoparticles can reduce the oxidative stress induced by doxorubicin on breast cancer cells.


Biologia ◽  
2018 ◽  
Vol 74 (2) ◽  
pp. 187-193 ◽  
Author(s):  
Richa Mehra ◽  
Satej Bhushan ◽  
Umesh Prasad Yadav ◽  
Felix Bast ◽  
Sandeep Singh

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