scholarly journals Multifunctional Gelatin/Chitosan Electrospun Wound Dressing Dopped with Undaria pinnatifida Phlorotannin-Enriched Extract for Skin Regeneration

Pharmaceutics ◽  
2021 ◽  
Vol 13 (12) ◽  
pp. 2152
Author(s):  
Carolina A. M. Ferreira ◽  
Adriana P. Januário ◽  
Rafael Félix ◽  
Nuno Alves ◽  
Marco F. L. Lemos ◽  
...  
Keyword(s):  
Wound Dressing ◽  
Undaria Pinnatifida ◽  
Healing Process ◽  
Skin Regeneration ◽  
Wound Dressings ◽  
Health Concern ◽  
Wound Healing Process ◽  
Hybrid Fibers ◽  
Degradation Rates ◽  
Over Time

The similarities of electrospun fibers with the skin extracellular matrix (ECM) make them promising structures for advanced wound dressings. Moreover, infection and resistance in wounds are a major health concern that may be reduced with antibacterial wound dressings. In this work, a multifunctional wound dressing was developed based on gelatin/chitosan hybrid fibers dopped with phlorotannin-enrich extract from the seaweed Undaria pinnatifida. The intrinsic electrospun structure properties combined with the antimicrobial and anti-inflammatory properties of phlorotannin-enrich extract will enhance the wound healing process. Electrospun meshes were produced by incorporating 1 or 2 wt% of extract, and the structure without extract was used as a control. Physico-chemical, mechanical, and biological properties were evaluated for all conditions. Results demonstrated that all developed samples presented a homogenous fiber deposition with the average diameters closer to the native ECM fibrils, and high porosities (~90%) that will be crucial to control the wound moist environment. According to the tensile test assays, the incorporation of phlorotannin-enriched extract enhances the elastic performance of the samples. Additionally, the extract incorporation made the structure stable over time since its in vitro degradation rates decreased under enzymatic medium. Extract release profile demonstrated a longstanding delivery (up to 160 days), reaching a maximum value of ~98% over time. Moreover, the preliminary antimicrobial results confirm the mesh’s antimicrobial activity against Pseudomonas aeruginosa and Staphylococcus aureus. In terms of biological characterization, no condition presented cytotoxicity effects on hDNF cells, allowing their adhesion and proliferation over 14 days, except the condition of 2 wt% after 7 days. Overall, the electrospun structure comprising phlorotannins-enriched extract is a promising bioactive structure with potential to be used as a drug delivery system for skin regeneration by reducing the bacterial infection in the wound bed.

scholarly journals Collagen-Based Nanofibers for Skin Regeneration and Wound Dressing Applications

Polymers ◽  
2021 ◽  
Vol 13 (24) ◽  
pp. 4368
Author(s):  
Zintle Mbese ◽  
Sibusiso Alven ◽  
Blessing Atim Aderibigbe
Keyword(s):  
Wound Healing ◽  
Wound Dressing ◽  
Cellular Proliferation ◽  
Healing Process ◽  
Skin Regeneration ◽  
Wound Dressings ◽  
Wound Management ◽  
Major Constituent ◽  
Wound Healing Process ◽  
Frequent Change

Skin regeneration after an injury is very vital, but this process can be impeded by several factors. Regenerative medicine is a developing biomedical field with the potential to decrease the need for an organ transplant. Wound management is challenging, particularly for chronic injuries, despite the availability of various types of wound dressing scaffolds in the market. Some of the wound dressings that are in clinical practice have various drawbacks such as poor antibacterial and antioxidant efficacy, poor mechanical properties, inability to absorb excess wound exudates, require frequent change of dressing and fails to offer a suitable moist environment to accelerate the wound healing process. Collagen is a biopolymer and a major constituent of the extracellular matrix (ECM), making it an interesting polymer for the development of wound dressings. Collagen-based nanofibers have demonstrated interesting properties that are advantageous both in the arena of skin regeneration and wound dressings, such as low antigenicity, good biocompatibility, hemostatic properties, capability to promote cellular proliferation and adhesion, and non-toxicity. Hence, this review will discuss the outcomes of collagen-based nanofibers reported from the series of preclinical trials of skin regeneration and wound healing.

scholarly journals Intelligent wound dressing for diagnostic & therapeutic applications - critical to wound infection

2020 ◽  
Vol 2 (7A) ◽  
Author(s):  
Zahid Mahmood ◽  
Anne-Marie Salisbury ◽  
Rui Chen ◽  
Stephen Rimmer ◽  
Steven Percival
Keyword(s):  
Wound Dressing ◽  
Antimicrobial Agents ◽  
Detection System ◽  
Healing Process ◽  
Wound Dressings ◽  
Core Material ◽  
Wound Healing Process ◽  
Polymer Complex ◽  
Triggered Release ◽  
Antibiofilm Agents

A medical device comprising of biomaterials responsive to biochemical stimuli: channel for indicating the infective states of wounds and ensuring delivery of smart antimicrobial and antibiofilm agents to promote tissue regeneration and healing. The importance of providing diagnostic wound dressings that can inform healthcare professionals on the state of infection within wounds but also provide some of the treatment required in response to at risk or infected wounds is of key interest. The aim is to investigate an innovative proof of concept diagnostic and detection system, an intelligent hydrogel wound dressing that responds to specific biochemical stimuli in wounds (MMPs and pH) enabling the selective and triggered release of antibiofilm and antimicrobial agents (‘Detect and Treat’)to the trauma site. The dressing is made of a sterile alginate core material covered in a biocompatible dry or hydrated peptide-polymer-complex film and may include a fluorescent dye which upon release during the wound healing process indicates when a change in dressing is necessary. Efficacy studies of the hydrogel dressing were performed within a drip-flow bioreactor in which regression of Pseudomonas aeruginosa biofilm was observed. A 5-log reduction in biofilm was observed in comparison to an untreated control biofilm. The hydrogel dressing indicated a clear response when in contact with biofilms produced only by pathogenic strains of bacteria when analysed. This further confirmed the adequate release and function of the antimicrobial and antibiofilm agents within the peptide-polymer-complex formulation of the hydrogel wound dressing.

scholarly journals Current Trends in Advanced Alginate-Based Wound Dressings for Chronic Wounds

2021 ◽  
Vol 11 (9) ◽  
pp. 890
Author(s):  
Andreea Barbu ◽  
Bogdan Neamtu ◽  
Marius Zăhan ◽  
Gabriela Mariana Iancu ◽  
Ciprian Bacila ◽  
...  
Keyword(s):  
Wound Healing ◽  
Chronic Inflammation ◽  
Wound Dressing ◽  
Chronic Wounds ◽  
Healing Process ◽  
Wound Dressings ◽  
In Vivo Studies ◽  
Public Health Issue ◽  
Wound Healing Process ◽  
Special Importance

Chronic wounds represent a major public health issue, with an extremely high cost worldwide. In healthy individuals, the wound healing process takes place in different stages: inflammation, cell proliferation (fibroblasts and keratinocytes of the dermis), and finally remodeling of the extracellular matrix (equilibrium between metalloproteinases and their inhibitors). In chronic wounds, the chronic inflammation favors exudate persistence and bacterial film has a special importance in the dynamics of chronic inflammation in wounds that do not heal. Recent advances in biopolymer-based materials for wound healing highlight the performance of specific alginate forms. An ideal wound dressing should be adherent to the wound surface and not to the wound bed, it should also be non-antigenic, biocompatible, semi-permeable, biodegradable, elastic but resistant, and cost-effective. It has to give protection against bacterial, infectious, mechanical, and thermal agents, to modulate the level of wound moisture, and to entrap and deliver drugs or other molecules This paper explores the roles of alginates in advanced wound-dressing forms with a particular emphasis on hydrogels, nanofibers networks, 3D-scaffolds or sponges entrapping fibroblasts, keratinocytes, or drugs to be released on the wound-bed. The latest research reports are presented and supported with in vitro and in vivo studies from the current literature.

scholarly journals Composite Wound Dressing Based on Chitin/Chitosan Nanofibers: Processing and Biomedical Applications

Cosmetics ◽  
2019 ◽  
Vol 6 (1) ◽  
pp. 16 ◽  
Author(s):  
Anton Shabunin ◽  
Vladimir Yudin ◽  
Irina Dobrovolskaya ◽  
Evgeny Zinovyev ◽  
Viktor Zubov ◽  
...  
Keyword(s):  
Wound Dressing ◽  
High Efficiency ◽  
Healing Process ◽  
Experimental Studies ◽  
Scar Tissue ◽  
Wound Dressings ◽  
Control Group ◽  
Wound Healing Process ◽  
Electrospinning Technique

An electrospinning technique was used for the preparation of a bilayered wound dressing consisting of a layer of aliphatic copolyamide nanofibers and a layer of composite nanofibers from chitosan and chitin nanofibrils filler. Processed dressings were compared with aliphatic copolyamide nanofiber-based wound dressings and control groups. Experimental studies (in vivo treatment of third-degree burns with this dressing) demonstrated that almost complete (up to 97.8%) epithelialization of the wound surface had been achieved within 28 days. Planimetric assessment demonstrated a significant acceleration of the wound healing process. Histological analysis of scar tissue indicated the presence of a significant number of microvessels and a low number of infiltrate cells. In the target group, there were no deaths or purulent complications, whereas in the control group these occurred in 25% and 59.7% of cases, respectively—and, in the copolyamide group, 0% and 11%, respectively. The obtained data show the high efficiency of application of the developed composite chitosan‒copolyamide wound dressings for the treatment of burn wounds.

Preparation and in vivo evaluation of a novel gel-based wound dressing using arginine–alginate surface-modified chitosan nanofibers

2017 ◽  
Vol 32 (6) ◽  
pp. 689-701 ◽  
Author(s):  
Mahsa Hoseinpour Najar ◽  
Mohsen Minaiyan ◽  
Azade Taheri
Keyword(s):  
Wound Healing ◽  
Wound Dressing ◽  
Healing Process ◽  
Wound Dressings ◽  
Wound Healing Process ◽  
In Vivo Evaluation ◽  
Animal Group ◽  
Modified Chitosan ◽  
Surface Modified

The development of an effective wound dressing with the ability to induce skin wound healing is a great challenge in medicine. Nanofibers are highly attractive for wound dressing preparation due to their properties such as hemostasis induction, good absorption of wound exudates, and facilitation of cell growth. Chitosan nanofibers have attracted great attention for application in wound dressings due to their accelerating effects on wound healing. In this study, arginine surface-modified chitosan nanofibers were successfully prepared by attachment of arginine molecules on the surface of chitosan nanofibers using sodium alginate through electrostatic interaction. The effect of pH on the amount of attached arginine was evaluated at three different pH values; 5, 6, and 7. Fourier-transform infrared spectroscopy and zeta potential of chitosan nanofibers before and after surface modification suggested the occurrence of the attachment of arginine to chitosan nanofibers. Scanning electron microscope images showed the nanofibrous structure of arginine surface-modified chitosan nanofibers with an average diameter ranging from 100 nm to 150 nm. The release of arginine from arginine surface-modified chitosan nanofibers gel showed a sustained release manner. The suitable viscosity and spreadability of arginine surface-modified chitosan nanofibers gel verified its easy application at the wound site. Arginine surface-modified chitosan nanofibers gel significantly improved the wound healing process including wound closure when tested in vivo using rat model. Additionally, histological examination and immunohistochemical studies showed the significant enhancement of the re-epithelialization, collagen deposition, and angiogenesis in the skin of the animal group treated with arginine surface-modified chitosan nanofibers gel compared with the other control groups. These results suggested that arginine surface-modified chitosan nanofibers gel could be introduced as an effective wound dressing.

Evaluation of a novel bioactive wound dressing: an in vitro and in vivo study

2021 ◽  
Vol 30 (6) ◽  
pp. 482-490
Author(s):  
Fahimeh Farshi Azhar ◽  
Paria Rostamzadeh ◽  
Monireh Khordadmehr ◽  
Mehran Mesgari-Abbasi
Keyword(s):  
Wound Healing ◽  
Wound Dressing ◽  
Healing Process ◽  
Wound Dressings ◽  
Bilayer Film ◽  
Wound Healing Process ◽  
Tissue Contraction ◽  
Experimental Rat Model

Objective: Hard-to-heal wounds, such as pressure ulcers and diabetic ulcers, are a major challenge for wound dressings. The aim of this study was to develop a bioactive dressing based on polymers and natural materials with unique biological and therapeutic properties. Method: The dressing was composed of an active layer containing polyvinyl alcohol (PVA), honey, curcumin and keratin, and an upper layer with lower hydrophilicity comprising PVA to induce flexibility. Physicochemical properties of the dressing were characterised by Fourier transform infrared spectroscopy, field emission scanning electron microscopy, swelling behaviour and antibacterial measurements. A wound healing study was performed using an experimental rat model and two different compositions of the bioactive dressing were compared with a commercial wound dressing (Comfeel, Coloplast, Denmark). Histopathological evaluation was conducted for this purpose. Results: Characterisation results showed that a smooth bilayer film with two homogenous but distinct layers was produced. The dressing also provided adequate moisture to the wound environment without infection and adhesion due to dryness occurring. Our results exhibited significant bactericidal activity against Gram-negative (Escherichia coli) and Gram-positive (Staphylococcus aureus) bacteria and improved the wound healing process without any scarring. Histopathological findings demonstrated a significant higher healing rate in vivo together with well-formed epidermis, granulation tissue formation and tissue contraction, when compared with the commercial wound dressing. Conclusion: Our results demonstrated acceptable physical and healing effects for the novel bioactive wound dressing; however, more investigations are recommended.

scholarly journals Nanotechnology Development for Formulating Essential Oils in Wound Dressing Materials to Promote the Wound-Healing Process: A Review

2021 ◽  
Vol 11 (4) ◽  
pp. 1713
Author(s):  
Ilenia De Luca ◽  
Parisa Pedram ◽  
Arash Moeini ◽  
Pierfrancesco Cerruti ◽  
Gianfranco Peluso ◽  
...  
Keyword(s):  
Wound Healing ◽  
Essential Oils ◽  
Wound Dressing ◽  
Healing Process ◽  
Wound Dressings ◽  
Wound Healing Process ◽  
Efficient Treatment ◽  
Anti Inflammatory ◽  
Dressing Materials ◽  
Wound Dressing Materials

Wound healing refers to the replacement of damaged tissue through strongly coordinated cellular events. The patient’s condition and different types of wounds complicate the already intricate healing process. Conventional wound dressing materials seem to be insufficient to facilitate and support this mechanism. Nanotechnology could provide the physicochemical properties and specific biological responses needed to promote the healing process. For nanoparticulate dressing design, growing interest has focused on natural biopolymers due to their biocompatibility and good adaptability to technological needs. Polysaccharides are the most common natural biopolymers used for wound-healing materials. In particular, alginate and chitosan polymers exhibit intrinsic antibacterial and anti-inflammatory effects, useful for guaranteeing efficient treatment. Recent studies highlight that several natural plant-derived molecules can influence healing stages. In particular, essential oils show excellent antibacterial, antifungal, antioxidant, and anti-inflammatory properties that can be amplified by combining them with nanotechnological strategies. This review summarizes recent studies concerning essential oils as active secondary compounds in polysaccharide-based wound dressings.

scholarly journals Macrophage immunomodulation: An indispensable tool to evaluate the performance of wound dressing biomaterials

2019 ◽  
Vol 17 (1) ◽  
pp. 228080001983035 ◽  
Author(s):  
Patrícia Varela ◽  
Susanna Sartori ◽  
Richard Viebahn ◽  
Jochen Salber ◽  
Gianluca Ciardelli
Keyword(s):  
Wound Healing ◽  
Immune Cells ◽  
Wound Dressing ◽  
Chronic Wounds ◽  
Macrophage Polarization ◽  
Healing Process ◽  
Wound Dressings ◽  
Wound Healing Process ◽  
Immunomodulatory Effects ◽  
Wide Range

A major burden of the healthcare system resides in providing proper medical treatment for all types of chronic wounds, which are usually treated with dressings to induce a faster regeneration. Hence, to reduce healing time and improve the patient’s quality of life, it is extremely important to select the most appropriate constituent material for a specific wound dressing. A wide range of wound dressings exist but their mechanisms of action are poorly explored, especially concerning the immunomodulatory effects that occur from the interactions between immune cells and the biomaterial. Tissue-resident and monocyte-derived recruited macrophages are key regulators of wound repair. These phagocytic immune cells exert specific functions during the different stages of wound healing. The recognition of the substantial role of macrophages in the outcome of the wound healing process requires specific understanding of the immunomodulatory effects of commercially available or newly developed wound dressings. For a precise intervention, it is necessary to obtain more knowledge on macrophage polarization in different phases of wound healing in the presence of the dressings. The main purpose of this review is to collect clinical cases in which macrophage immunomodulation was taken into consideration as an indicator of the performances of novel or mainstream wound dressing materials, including those provided with antimicrobial properties.

scholarly journals Modern Wound Dressings and their Application

2019 ◽  
Author(s):  
Maliheh Yaghoobi ◽  
Masoumeh Nasrollahzadeh ◽  
Fatemeh Asjadi
Keyword(s):  
Wound Healing ◽  
Wound Dressing ◽  
Healing Process ◽  
Wound Dressings ◽  
Wound Healing Process ◽  
Physical Interaction ◽  
Factors Affecting ◽  
Comprehensive Information ◽  
Selection Of ◽  
Suitable Environment

Introdution: Wound healing is a complex and dynamic process, which requires to be prepared a suitable environment to promote healing process. Wound dressing is one of the most important external factors affecting the wound healing process. With the advancement in technology, more than 1000 types of modern wound dressings have been developed. Wound dressings are either based on natural materials or on the basis of synthetic and chemical materials. In addition to the protective and covering role, which modern wound dressings play, they have a chemical and physical interaction with wound bed addition. They carry antibiotics, drugs and nanoparticles and play an important role in maintaining moisture or hydration the wound; all of these factors can facilitate the wound healing process. In this study, the wound healing process and the properties of wound dressings were presented first and then the types of wound dressings and their applications were investigated. This study, with comprehensive information on the types of modern wound dressings and their advantages and drawbacks, will help a physician in the proper selection of an appropriate wound dressing.

scholarly journals Hydrogel Preparation Methods and Biomaterials for Wound Dressing

Life ◽  
2021 ◽  
Vol 11 (10) ◽  
pp. 1016
Author(s):  
Jingjing Su ◽  
Jiankang Li ◽  
Jiaheng Liang ◽  
Kun Zhang ◽  
Jingan Li
Keyword(s):  
Wound Dressing ◽  
Wound Repair ◽  
Chronic Wounds ◽  
Healing Process ◽  
3D Structure ◽  
Skin Wound ◽  
Wound Dressings ◽  
Wound Healing Process ◽  
Skin Wound Healing ◽  
Preparation Methods

Wounds have become one of the causes of death worldwide. The metabolic disorder of the wound microenvironment can lead to a series of serious symptoms, especially chronic wounds that bring great pain to patients, and there is currently no effective and widely used wound dressing. Therefore, it is important to develop new multifunctional wound dressings. Hydrogel is an ideal dressing candidate because of its 3D structure, good permeability, excellent biocompatibility, and ability to provide a moist environment for wound repair, which overcomes the shortcomings of traditional dressings. This article first briefly introduces the skin wound healing process, then the preparation methods of hydrogel dressings and the characteristics of hydrogel wound dressings made of natural biomaterials and synthetic materials are introduced. Finally, the development prospects and challenges of hydrogel wound dressings are discussed.

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