scholarly journals Pretreatment with H2O2 Alleviates the Negative Impacts of NaCl Stress on Seed Germination of Tartary Buckwheat (Fagopyrum tataricum)

Plants ◽  
2021 ◽  
Vol 10 (9) ◽  
pp. 1784
Author(s):  
Xin Yao ◽  
Meiliang Zhou ◽  
Jingjun Ruan ◽  
Yan Peng ◽  
Hao Yang ◽  
...  
Keyword(s):  
Superoxide Dismutase ◽  
Salt Tolerance ◽  
Seed Germination ◽  
Superoxide Dismutase Activity ◽  
Stress Factors ◽  
Tartary Buckwheat ◽  
Tolerance Index ◽  
Fagopyrum Tataricum ◽  
Germination Parameters ◽  
H2o2 Pretreatment

Soil salinization is one of the main abiotic stress factors impacting the growth of crops and the agricultural industry today. Thus, we aimed to investigate the effects of H2O2 pretreatment on seed germination in Tartary buckwheat (Fagopyrum tataricum) seeds under salt stress and to evaluate this species’ salt tolerance. Through the preliminary experiment, this study used 50 mmol L−1 NaCl solution to induce seed stress. After soaking for 12 h in different H2O2 concentrations, seeds were laid in Petri dishes with 50 mmol L−1 NaCl for seven days and the germination parameters and physiological indicators were measured to screen the optimal H2O2 pretreatment concentration and the salt tolerance index. Our results indicated that pretreatment with 5–10 mmol L−1 H2O2 was most effective in alleviating NaCl’s impacts on the seeds’ germination parameters. Furthermore, the growth and material accumulation of seedlings was promoted; catalase, superoxide dismutase activity, and proline content were enhanced; and malondialdehyde content was reduced. Principal component analysis and stepwise regression revealed six key indicators that had a significant impact on the salt tolerance characteristics of F. tataricum, namely, germination potential, shoot fresh weight, root surface area, root average diameter, catalase activity, and superoxide dismutase activity.

Responses of seed germination, seedling growth under salinity stresses and variability for phenotypic traits in Tossa Jute (Corchorus olitorius L.)

2021 ◽  
Vol 8 (1) ◽  
Author(s):  
Md. Mia Mukul ◽  
Sheikh Shorif Uddin Ahmed ◽  
Nargis Akter ◽  
Md. Golam Mostofa ◽  
Md. Sohanur Rahman ◽  
...  
Keyword(s):  
Salt Tolerance ◽  
Seed Germination ◽  
Germination Rate ◽  
Phenotypic Traits ◽  
Tolerance Index ◽  
Corchorus Olitorius ◽  
Salt Tolerant ◽  
Fiber Yield ◽  
Good For ◽  
Salt Tolerance Index

Salinity is a serious abiotic stress to Jute and other crop cultivation at saline regions in the world. No salt tolerant Tossa Jute (Corchorus olitorius L.) variety was developed in Bangladesh. Hence, six Tossa Jute accessions were investigated at germination stage against six concentration levels (0.00 or d.H2O, 8.0, 10.0, 12.0, 14.0 and 16.0 dS m-1) of salt (NaCl) using RCB design at Bangladesh Jute Research Institute (BJRI) during March-July, 2020. Jute seeds collected from Gene Bank of BJRI were allowed to germinate under laboratory condition. Seed germination rate was adversely affected as well as delay in germination was prolonged with increasing the salt concentration. In control, seeds were germinated up to 14.0 dS m-1 salt solution. Among six genotypes, Acc. 1141 and Acc. 3801 showed the highest germination rate (86.67 %); Acc. 3801 gave maximum root length (17.0 mm), dry biomass (6.37 mg); and Acc. 1089 showed higher shoot length (10.0 mm), fresh weight (43.93 mg) and salt tolerance index (60.69 %) under 14.0 dS m-1 level. Higher relative salt harm rate (7.14 %) was observed in both Acc. 1141 and Acc. 3801 under 14.0 dS m-1 salinity indicating highly tolerance to salinity. Acc. 3801 and Acc. 1141 were found good for germination under salt stresses; Acc. 3801, Acc. 1089 for fiber yield and salt tolerance; Acc. 3801 and Acc. 1407 for higher fiber yield. Acc. 3801 was found good for salt tolerance and fiber yield content. The genotypes with good desirable characters would be used as breeding materials to develop high yielding salt tolerant Tossa Jute variety.

scholarly journals Salt Resistance of Tomato (Lycopersicon esculentum Mill.) Cultivars Produced in Benin at Germination Stage

2019 ◽  
pp. 1-12
Author(s):  
Eliane Kinsou ◽  
David Montcho ◽  
Séraphin Ahissou Zanklan ◽  
Julien Koffi Kpinkoun ◽  
Françoise Assogba Komlan ◽  
...  
Keyword(s):  
Salt Tolerance ◽  
Seed Germination ◽  
Germination Rate ◽  
Nacl Stress ◽  
Salt Resistance ◽  
Tolerance Index ◽  
Resistance Level ◽  
Significant Difference ◽  
Salt Tolerance Index ◽  
Germination Stage

Aims: In this research study, salt resistance level of seven tomato cultivars grown in Benin, namely Akikon, Tounvi; F1 Mongal, Petomech, Padma, TLCV 15 and Thorgal was evaluated at the germination stage. Study Design: The experiment was laid out as a completely randomized design with four replications. Place and Duration of Study: The experiment was carried out in the Laboratory of Plant Physiology and Abiotic Stresses Study of University of Abomey-Calavi, Republic of Benin from May to June, 2017. Methodology: Seeds were submitted to treatment with four NaCl concentrations (0; 30; 60 and 90 mM NaCl) in Petri dishes. Seed germination was checked every day during ten days incubation period. Four replicates of 40 seeds each were used. Results: NaCl reduced seed germination rate in all cultivars from day 2 to day 10 and the germination index proportionately to NaCl concentration. At the end of the 10 days, salt stress reduced the final germination percentages with a significant difference among cultivars: cultivars F1 Mongal followed by Akikon, Thorgal, TLCV15 and Tounvi were less affected in comparison with the two other cultivars. Salt Tolerance Index was significantly variable according to the cultivar with the highest values for cultivars F1 Mongal (1.086), Akikon (1.028), TLCV15 (1.005) and Tounvi (0.989) and the weakest value for cultivar Petomech (0.436). Conclusion: NaCl stress delayed seed germination and reduced the rate of final germination. Salt Tolerance Index was variable among the seven cultivars. Based on this criterion, cultivars F1 Mongal, Akikon, TLCV15 and Tounvi were the most salt-resistant whereas Petomech was the most salt-sensitive at germination stage.

scholarly journals Evaluation of Salt Tolerance at Germination Stage in Cowpea [Vigna unguiculata (L.) Walp]

HortScience ◽  
2017 ◽  
Vol 52 (9) ◽  
pp. 1168-1176 ◽  
Author(s):  
Waltram Second Ravelombola ◽  
Ainong Shi ◽  
Yuejin Weng ◽  
John Clark ◽  
Dennis Motes ◽  
...  
Keyword(s):  
Salt Tolerance ◽  
Seed Germination ◽  
Salinity Stress ◽  
Germination Rate ◽  
Correlation Coefficients ◽  
Human Consumption ◽  
Tolerance Index ◽  
Salt Tolerant ◽  
Salt Tolerance Index ◽  
Germination Stage

Cowpea is a leguminous and versatile crop which provides nutritional food for human consumption. However, salinity unfavorably reduces cowpea seed germination, thus significantly decreasing cowpea production. Little has been done for evaluating and developing salt-tolerant cowpea genotypes at germination stage. The objectives of this research were to evaluate the response of cowpea genotypes to salinity stress through seed germination rate and to select salt-tolerant cowpea genotypes. The seed germination rates under nonsalt condition and salinity stress (150 mm NaCl) were evaluated in 151 cowpea genotypes. Four parameters, absolute decrease (AD), the inhibition index (II), the relative salt tolerance (RST), and the salt tolerance index (STI) were used to measure salt tolerance in cowpea. The results showed that there were significant differences among the 151 cowpea genotypes for all parameters (P values <0.0001). The AD in germination rate was 5.8% to 94.2%; the II varied from 7.7% to 100%; the RST ranged from 0 to 0.92; and STI varied from 0 to 0.92. A high broad sense heritability (H2) was observed for all four parameters. High correlation coefficients (r) were estimated among the four parameters. PI582422, 09–529, PI293584, and PI582570 were highly salt tolerant at germination stage. In addition, genotypes from the Caribbean and Southern Asia exhibited better tolerance to salinity, whereas those from Europe and North America were the most salt-susceptible.

scholarly journals Multivariate Analysis as a Tool for Studying the Effects of Salinity in Different Melon Landraces at Germination Stage

2016 ◽  
Vol 44 (1) ◽  
pp. 264-271 ◽  
Author(s):  
Behrooz SARABI ◽  
Sahebali BOLANDNAZAR ◽  
Nasser GHADERI ◽  
Seyed Jalal TABATABAEI
Keyword(s):  
Principal Component Analysis ◽  
Salt Tolerance ◽  
Seed Germination ◽  
Seedling Growth ◽  
Principal Component ◽  
Component Analysis ◽  
Growth Stages ◽  
Tolerance Index ◽  
Plant Seed ◽  
Salt Tolerance Index

Although salt stress affects all growth stages of a plant, seed germination and seedling growth stages are known to be more sensitive for most plant species. The current research was carried out to find the most effective indicators when screening melons for salt tolerance. For this purpose, a factorial experiment as a completely randomized design was performed in order to investigate the effects of salinity (0, 20, 40, 60 and 80 mM NaCl) on seed germination parameters, hydrogen peroxide content, lipid peroxidation and some antioxidant enzymes (guaiacol peroxidase, ascorbate peroxidase and superoxide dismutase) in five Iranian melon landraces and one melon cultivar ‘Galia’ F1 at early seedling growth stage. The ‘Galia’ F1 was included for comparison as a salt-tolerant cultivar. The data collected from all salinity treatments across six landraces were used for analysis of variance and principal component analysis (PCA) using SPSS software as well as for obtaining Pearson’s correlation coefficients (r) among all physiological parameters. Obtained results indicated that averaged all landraces across all treatments, increased NaCl significantly affected all the parameters measured. In all landraces, salt tolerance index (STI) decreased as the salt concentrations increased. The principal component analysis revealed variations among the traits and determined four main factors that explained 97.53% of the total variance. Simple correlation coefficient analyses showed the existence of significant positive and negative correlations among characteristics. Our study suggests that the salt tolerance index and principal component analysis can be used as accurate and easy indicators when screening melons for salt tolerance.

STUDIES ON SEED DORMANCY, PLANT DEVELOPMENT, AND CHEMICAL CONTROL OF TARTARY BUCKWHEAT (FAGOPYRUM TATARICUM (L.) GAERTN.).: II. GERMINATION, GROWTH, FLOWERING AND SEED PRODUCTION

1958 ◽  
Vol 38 (3) ◽  
pp. 367-373 ◽  
Author(s):  
William H. Vanden Born ◽  
William G. Corns
Keyword(s):  
Seed Germination ◽  
Seed Production ◽  
Plant Development ◽  
Environmental Conditions ◽  
Chemical Control ◽  
Growing Season ◽  
Tartary Buckwheat ◽  
Fagopyrum Tataricum ◽  
Wide Range ◽  
Vegetative Matter

Seed germination, plant development, flowering and seed production of Tartary buckwheat (Fagopyrum tataricum) were observed under various environmental conditions. After-ripened seeds germinated over a wide range of temperatures under laboratory conditions, and in the field produced seedlings whenever tested during the growing season. Shoots were sent up from depths up to 6 inches. Flowering began 5 to 6 weeks after planting, and the first seeds matured 4 to 5 weeks later. Flowering and seed production continued until stopped by frost in the fall. Yields of vegetative matter and seed were recorded. Abnormal seeds produced by buckwheat plants treated with low volatile (LV) 2,4-D were as viable as were normal seeds, and when planted in the field the following year they produced plants which were normal in all respects.

Development of Rutin-rich Noodles Using Trace-rutinosidase Variety of Tartary Buckwheat (Fagopyrum Tataricum Gaertn.) ‘Manten-Kirari’

2019 ◽  
Vol 25 (6) ◽  
pp. 915-920
Author(s):  
Tatsuro Suzuki ◽  
Toshikazu Morishita ◽  
Shigenobu Takigawa ◽  
Takahiro Noda ◽  
Koji Ishiguro
Keyword(s):  
Tartary Buckwheat ◽  
Fagopyrum Tataricum

Diazoxide Modulates Cardiac Hypertrophy by Targeting H2O2 Generation and Mitochondrial Superoxide Dismutase Activity

2020 ◽  
Vol 13 (1) ◽  
pp. 76-83
Author(s):  
Aline Maria Brito Lucas ◽  
Joana Varlla de Lacerda Alexandre ◽  
Maria Thalyne Silva Araújo ◽  
Cicera Edna Barbosa David ◽  
Yuana Ivia Ponte Viana ◽  
...  
Keyword(s):  
Oxidative Stress ◽  
Superoxide Dismutase ◽  
Cardiac Hypertrophy ◽  
Superoxide Dismutase Activity ◽  
Heart Weight ◽  
Pharmacological Intervention ◽  
Wall Thickening ◽  
H2o2 Production ◽  
Mitochondrial Superoxide ◽  
Mitochondrial Superoxide Dismutase

Background: Cardiac hypertrophy involves marked wall thickening or chamber enlargement. If sustained, this condition will lead to dysfunctional mitochondria and oxidative stress. Mitochondria have ATP-sensitive K+ channels (mitoKATP) in the inner membrane that modulate the redox status of the cell. Objective: We investigated the in vivo effects of mitoKATP opening on oxidative stress in isoproterenol- induced cardiac hypertrophy. Methods: Cardiac hypertrophy was induced in Swiss mice treated intraperitoneally with isoproterenol (ISO - 30 mg/kg/day) for 8 days. From day 4, diazoxide (DZX - 5 mg/kg/day) was used in order to open mitoKATP (a clinically relevant therapy scheme) and 5-hydroxydecanoate (5HD - 5 mg/kg/day) or glibenclamide (GLI - 3 mg/kg/day) were used as mitoKATP blockers. Results: Isoproterenol-treated mice had elevated heart weight/tibia length ratios (HW/TL). Additionally, hypertrophic hearts had elevated levels of carbonylated proteins and Thiobarbituric Acid Reactive Substances (TBARS), markers of protein and lipid oxidation. In contrast, mitoKATP opening with DZX avoided ISO effects on gross hypertrophic markers (HW/TL), carbonylated proteins and TBARS, in a manner reversed by 5HD and GLI. Moreover, DZX improved mitochondrial superoxide dismutase activity. This effect was also blocked by 5HD and GLI. Additionally, ex vivo treatment of isoproterenol- induced hypertrophic cardiac tissue with DZX decreased H2O2 production in a manner sensitive to 5HD, indicating that this drug also acutely avoids oxidative stress. Conclusion: Our results suggest that diazoxide blocks oxidative stress and reverses cardiac hypertrophy. This pharmacological intervention could be a potential therapeutic strategy to prevent oxidative stress associated with cardiac hypertrophy.

scholarly journals Salinity Effects on Gene Expression, Morphological, and Physio-Biochemical Responses of Stevia rebaudiana Bertoni In Vitro

Plants ◽  
2021 ◽  
Vol 10 (4) ◽  
pp. 820
Author(s):  
Clara Azzam ◽  
Sudad Al-Taweel ◽  
Ranya Abdel-Aziz ◽  
Karim Rabea ◽  
Alaa Abou-Sreea ◽  
...  
Keyword(s):  
Salt Tolerance ◽  
Biochemical Parameters ◽  
Large Scale ◽  
Stevia Rebaudiana ◽  
Multiple Shoots ◽  
High Salt ◽  
Stress Factors ◽  
Stevia Rebaudiana Bertoni ◽  
Nacl Concentration

Stevia rebaudiana Bertoni is a little bush, which is cultivated on a large scale in many countries for medicinal purposes and used as a natural sweetener in food products. The present work aims to conduct a protocol for stevia propagation in vitro to produce and introduce Stevia rebaudiana plants as a new sweetener crop to Egyptian agriculture. To efficiently maximize its propagation, it is important to study the influence of stress factors on the growth and development of Stevia rebaudiana grown in vitro. Two stevia varieties were investigated (Sugar High A3 and Spanti) against salt stress. Leaves were used as the source of explants for callus initiation, regeneration, multiplication and rooting. Some stress-related traits, i.e., photosynthetic pigments, proline contents, and enzyme activity for peroxidase (POD), polyphenol oxidase (PPO), and malate dehydrogenase (MDH) were studied. Murashig and Skoog (MS) medium was supplemented with four NaCl concentrations: 500, 1000, 2000, and 3000 mgL−1, while a salt-free medium was used as the control. The data revealed that salinity negatively affected all studied characters: the number of surviving calli, regeneration%, shoot length, the number of multiple shoots, number of leaf plantlets−1, number of root plantlets−1, and root length. The data also revealed that Sugar High A3 is more tolerant than Spanti. The total chlorophyll content decreased gradually with increasing NaCl concentration. However, the opposite was true for proline content. Isozyme’s fractionation exhibited high levels of variability among the two varieties. Various biochemical parameters associated with salt tolerance were detected in POD. Namely, POD4, POD6, POD 9 at an Rf of 0.34, 0.57, and 0.91 in the Sugar High A3 variety under high salt concentration conditions, as well as POD 10 at an Rf of 0.98 in both varieties under high salt concentrations. In addition, the overexpression of POD 5 and POD 10 at Rf 0.52 and 0.83 was found in both varieties at high NaCl concentrations. Biochemical parameters associated with salt tolerance were detected in PPO (PPO1, PPO2 and PPO4 at an Rf of 0.38, 0.42 and 0.62 in the Sugar High A3 variety under high salt concentrations) and MDH (MDH 3 at an Rf of 0.40 in both varieties at high salt concentrations). Therefore, these could be considered as important biochemical markers associated with salt tolerance and could be applied in stevia breeding programs (marker-assisted selection). This investigation recommends stevia variety Sugar High A3 to be cultivated under salt conditions.

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