scholarly journals Introgression of Bacterial Blight Resistance Genes in the Rice Cultivar Ciherang: Response against Xanthomonas oryzae pv. oryzae in the F6 Generation

Plants ◽  
2021 ◽  
Vol 10 (10) ◽  
pp. 2048
Author(s):  
Priya Lal Biswas ◽  
Ujjal Kumar Nath ◽  
Sharmistha Ghosal ◽  
Gayatri Goswami ◽  
Md. Shalim Uddin ◽  
...  
Keyword(s):  
Resistance Genes ◽  
Bacterial Blight ◽  
Single Gene ◽  
Rice Variety ◽  
Rice Cultivar ◽  
Xanthomonas Oryzae ◽  
Yield Potential ◽  
High Yield ◽  
Bacterial Strains ◽  
Broad Spectrum Resistance

Bacterial blight (BB) is caused by Xanthomonas oryzae pv. oryzae and is one of the most important diseases in rice. It results in significantly reduced productivity throughout all rice-growing regions of the world. Four BB resistance genes have been reported; however, introgression of a single gene into rice has not been able to sufficiently protect rice against BB infection. Pyramiding of effective BB resistance genes (i.e., Xa genes) into background varieties is a potential approach to controlling BB infection. In this study, combinations of four BB resistance genes, Xa4, xa5, xa13, and Xa21, were pyramided into populations. The populations were derived from crossing Ciherang (a widespread Indonesian rice variety) with IRBB60 (resistance to BB). Promising recombinants from the F6 generation were identified by scoring the phenotype against three virulent bacterial strains, C5, P6, and V, which cause widespread BB infection in most rice-growing countries. Pyramiding of genes for BB resistance in 265 recombinant introgressed lines (RILs) were confirmed through marker-assisted selection (MAS) of the F5 and F6 generations using gene-specific primers. Of these 265 RILs, 11, 34 and 45 lines had four, three, or two BB resistance genes, respectively. The RILs had pyramiding of two or three resistance genes, with the Xa4 resistance gene showing broad spectrum resistance against Xoo races with higher agronomic performance compared to their donor and recipients parents. The developed BB-resistant RILs have high yield potential to be further developed for cultivation or as sources of BB resistance donor material for varietal improvement in other rice lines.

scholarly journals Marker assisted introgression of bacterial blight resistant gene into submergence tolerance rice variety BRRI dhan52

2017 ◽  
Vol 42 (3) ◽  
pp. 403-411 ◽  
Author(s):  
ME Kabir ◽  
KM Iftekharuddaula ◽  
MAI Khan ◽  
MAK Mian ◽  
NA Ivy
Keyword(s):  
Resistance Genes ◽  
Bacterial Blight ◽  
Flash Flood ◽  
Rice Variety ◽  
High Yield ◽  
Recurrent Parent ◽  
Breeding Lines ◽  
Resistant Gene ◽  
Rainfed Lowland ◽  
Elite Breeding Lines

BRRI dhan52 is a uniquire submergence tolerant rice variety containing prominant genetic background of BR11, a mega rainfed lowland rice (RLR) variety of Bangladesh, but is susceptible to bacterial blight (BB) caused by Xanthomonas oryzae pv. oryzae (Xoo). The variety is considerably popular in the southern part of Bangladesh due to its high yield in flash flood condition and comparatvely medium slender grain. Molecular markers linked to BB resistance genes (Xa genes) and submergence QTL (SUB1) were utilized in a marker-aided selection program to develop elite breeding lines with broad-spectrum resistance to bacterial blight. Sequence tagged site (STS) and simple sequence repeat (SSR) markers were essentially used to detect the genes for BB and submergence as well. In backcross generation, markers closely linked to Xa21 and SUB1 QTL were used to select desirable plants possessing these resistance genes (foreground selection) and microsatellite markers polymorphic between donor and recurrent parent were also used to select plants that have maximum contribution from the recurrent parent genome (background selection). In BC1F1 generation, three best plants consiquently were selected from previously selected ten double heterozygous (Xa21 and SUB1 QTL) plants. The percentage of recipient genome recovery in the best plant 1, 2 and 3 were 78.7%, 75.83% and 75.4%, respectively. Eventually this work illustrates the successful application of marker-assisted breeding for introgression of bacterial blight resistant gene into a rice variety of Bangladesh.Bangladesh J. Agril. Res. 42(3): 403-411, September 2017

scholarly journals Cloning of the rice Xo1 resistance gene and interaction of the Xo1 protein with the defense-suppressing Xanthomonas effector Tal2h

2020 ◽  
Author(s):  
Andrew C. Read ◽  
Mathilde Hutin ◽  
Matthew J. Moscou ◽  
Fabio C. Rinaldi ◽  
Adam J. Bogdanove
Keyword(s):  
Resistance Gene ◽  
Resistance Genes ◽  
Bacterial Blight ◽  
Rice Variety ◽  
Susceptible Variety ◽  
Xanthomonas Oryzae ◽  
Physical Interaction ◽  
Bacterial Leaf Streak ◽  
Transcription Activator ◽  
Rice Leaves

AbstractThe Xo1 locus in the heirloom rice variety Carolina Gold Select confers resistance to bacterial leaf streak and bacterial blight, caused by Xanthomonas oryzae pvs. oryzicola and oryzae, respectively. Resistance is triggered by pathogen-delivered transcription activator-like effectors (TALEs) independent of their ability to activate transcription, and is suppressed by variants called truncTALEs common among Asian strains. By transformation of the susceptible variety Nipponbare, we show that one of 14 nucleotide-binding, leucine-rich repeat (NLR) protein genes at the locus, with a zfBED domain, is the Xo1 gene. Analyses of published transcriptomes revealed that the Xo1-mediated response is similar to those of NLR resistance genes Pia and Rxo1 and distinct from that associated with induction of the executor resistance gene Xa23, and that a truncTALE dampens or abolishes activation of defense-associated genes by Xo1. In Nicotiana benthamiana leaves, fluorescently-tagged Xo1 protein, like TALEs and truncTALEs, localized to the nucleus. And, endogenous Xo1 specifically co-immunoprecipitated from rice leaves with a pathogen-delivered, epitope-tagged truncTALE. These observations suggest that suppression of Xo1-function by truncTALEs occurs through direct or indirect physical interaction. They further suggest that effector co-immunoprecipitation may be effective for identifying or characterizing other resistance genes.

scholarly journals Marker-assisted selection for transfer of submergence tolerance, bacterial blight resistance and yield enhancement in the rice backcross derivatives

2020 ◽  
pp. 1288-1294
Author(s):  
Shibani Mohapatra ◽  
Akshaya Kumar Bastia ◽  
Alok Kumar Panda ◽  
Sharat Kumar Pradhan
Keyword(s):  
Resistance Genes ◽  
Bacterial Blight ◽  
Rice Variety ◽  
Yield Component ◽  
Yield Enhancement ◽  
Yield Potential ◽  
Blight Resistance ◽  
Submergence Tolerance ◽  
Bacterial Blight Resistance ◽  
Rice Varieties

Achieving the demand and supply of rice for the increasing global population will be a challenging task beyond 2040. Therefore, the yield potential of popular rice varieties needs to be increased. The production from lowland rice can be enhanced by managing the major biotic and abiotic stresses faced in the ecology. In this work, we have developed the pipelines for gene stacking of submergence tolerance, bacterial blight resistance and yield component QTLs in the popular rice variety ‘Swarna’ by marker-assisted backcross breeding approach. Genotyping results of 796 BC1F1 backcross derived lines showed the presence of GW2, OsSPL14, SCM2 yield component QTLs, Sub1A for submergence tolerance and bacterial blight resistance genes xa5 and Xa21 in the progenies. The four stacked QTLs and two resistance genes were observed in six backcrosses derived progenies only. These progenies will be used in the next generation for further selection and evaluation for enhancing submergence tolerance, yield potential and bacterial blight resistance in Swarna rice variety background.

scholarly journals Pyramiding of three bacterial blight resistance genes for broad-spectrum resistance in deepwater rice variety, Jalmagna

Rice ◽  
2015 ◽  
Vol 8 (1) ◽  
Author(s):  
Sharat Kumar Pradhan ◽  
Deepak Kumar Nayak ◽  
Soumya Mohanty ◽  
Lambodar Behera ◽  
Saumya Ranjan Barik ◽  
...  
Keyword(s):  
Resistance Genes ◽  
Broad Spectrum ◽  
Bacterial Blight ◽  
Rice Variety ◽  
Blight Resistance ◽  
Bacterial Blight Resistance ◽  
Deepwater Rice ◽  
Broad Spectrum Resistance

scholarly journals Analisis Molekuler dan Keragaan Agronomis Galur-galur Padi BC1F1 Persilangan Code x qTSN4 dan Code x qDTH8 (Molecular Analysis and Agronomic Performance of BC1F1 Crosses Code x qTSN4 and Code x qDTH8)

2016 ◽  
Vol 11 (1) ◽  
pp. 17
Author(s):  
Tasliah Tasliah ◽  
Ma'sumah Ma'sumah ◽  
Kurniawan R. Trijatmiko ◽  
Joko Prasetiyono
Keyword(s):  
Bacterial Blight ◽  
Rice Variety ◽  
Routine Activity ◽  
Yield Potential ◽  
High Yield ◽  
Recurrent Parent ◽  
Bacterial Blight Resistance ◽  
Plant Materials ◽  
Days To Heading ◽  
Materials Used

<p>Breeding based on molecular marker has become a routine activity in the current rice research. The development of an early<br />maturity of rice variety with high yield is needed to increase national rice production. This study aimed to determine the pattern<br />of alleles for loci controlling total spikelet number and number of days to heading, as well as agronomic performances of the<br />BC1F1 Code x qTSN4 and Code x qDTH8 populations. The study was conducted at the Indonesian Center for Biotechnology and<br />Genetic Resources Research and Development from January to August 2014. The plant materials used were Code (a national<br />variety with bacterial blight resistance gene [Xa7]), IR64-Nils-qTSN4[YP9] (qTSN4 that contains a locus controlling the number of<br />spikelet), IR64-Nils-qDTH8[YP1] (qDTH8 that contains a locus controlling the number of days to heading), BC1F1 Code x qTSN4,<br />and BC1F1 Code x qDTH8. A total of 250 BC1F1 plants of each crosses were selected using molecular markers of RM20582 for Xa7<br />gene, RM17483 and RM6909 for QTL position of qTSN4, RM5556 and RM6838 for QTL position of qDTH8. Based on molecular<br />analysis, there were 63 BC1F1-qTSN4 lines and 65 BC1F1-qDTH8 lines showing heterozygote alleles for qTSN4 or qDTH8 loci and<br />were homozygote for Xa7 locus (HHA pattern). Five plants from each locus target were backcrossed to the recurrent parent,<br />Code, to obtain BC2F1 seeds. The remaining BC1F1 plants were self-pollinated to obtain BC1F2 seeds. Observations on some<br />agronomic characters demontrated that the BC1F1 plants showed higher yield potential than Code and the flowering time of the<br />BC1F1 progenis were also earlier than Code. These results indicated that the yield potential of Code could be improved by<br />introgression of qTSN4 and qDTH8 loci into the Code genome.</p>

Evaluation of resistance of rice genotypes (derived from the cross between HKR-127 and IRBB-60) against bacterial blight caused by Xanthomonas oryzae pv. oryzae

2019 ◽  
Vol 9 (4) ◽  
pp. 142-147
Author(s):  
Kirti Mehta ◽  
Nikita Baliyan ◽  
Rahul Kumar Meena ◽  
Shikha Yashveer
Keyword(s):  
Resistance Genes ◽  
Evaluation System ◽  
Bacterial Blight ◽  
Pathogenic Bacteria ◽  
Rice Variety ◽  
Xanthomonas Oryzae ◽  
Lesion Length ◽  
Bacterial Diseases ◽  
Agricultural Produce ◽  
Rice Genotypes

Rice crop is susceptible to various bacterial diseases and one such commonly known disease is Bacterial Blight (BB) caused by the pathogenic bacteria Xanthomonas oryzae pv. oryzae (Xoo). Rice variety HKR-127 is widely accepted by rice farmers, users and buyers in Haryana (India) owing to its high agricultural produce, medium lanky grains, and outstanding cooking characteristics, however, HKR-127 exhibits less endurance to BB. The aim of the study conducted at CCS Haryana Agricultural University was to investigate the genetic potential of BC3F2 and BC3F3 pyramided rice genotypes (cross HKR-127 x IRBB -60) having resistance genes (Xa21, xa13, and xa5). These genotypes were tested for virulence against BB under artificial conditions using Clip method of artificial inoculation. On average, five leaves per plant were inoculated and visually scored after 14 days. Disease reaction rating was done on a 0-9 scale of the standard evaluation system (SES) for rice. Rice genotypes with all the three genes exhibited relatively low mean lesion length compared to single or double combinations thus establishing higher resistance of threegene genotypes to BB. The lines obtained in our study can be used as genetic resources for BB resistance in breeding programs that will be paving the way for an environmentally-friendly means to achieve a better disease management. Moreover, the success will facilitate future efforts to transfer combinations of BB resistance genes into other preferred rice cultivars. 

scholarly journals Dissection of broad-spectrum resistance of the Thai rice variety Jao Hom Nin conferred by two resistance genes against rice blast

Rice ◽  
2017 ◽  
Vol 10 (1) ◽  
Author(s):  
Chaivarakun Chaipanya ◽  
Mary Jeanie Telebanco-Yanoria ◽  
Berlaine Quime ◽  
Apinya Longya ◽  
Siripar Korinsak ◽  
...  
Keyword(s):  
Resistance Genes ◽  
Broad Spectrum ◽  
Rice Blast ◽  
Rice Variety ◽  
Broad Spectrum Resistance

scholarly journals The Role of a Host-Induced Arginase of Xanthomonas oryzae pv. oryzae in Promoting Virulence on Rice

2019 ◽  
Vol 109 (11) ◽  
pp. 1869-1877
Author(s):  
Yuqiang Zhang ◽  
Guichun Wu ◽  
Ian Palmer ◽  
Bo Wang ◽  
Guoliang Qian ◽  
...  
Keyword(s):  
Biofilm Formation ◽  
Bacterial Blight ◽  
Wild Type Strain ◽  
Bacterial Pathogen ◽  
Extracellular Polysaccharide ◽  
Rice Cultivar ◽  
Xanthomonas Oryzae ◽  
Wild Type ◽  
Bacterial Blight Of Rice

The plant bacterial pathogen Xanthomonas oryzae pv. oryzae causes bacterial blight of rice, which is one of the most destructive rice diseases prevalent in Asia and parts of Africa. Despite many years of research, how X. oryzae pv. oryzae causes bacterial blight of rice is still not completely understood. Here, we show that the loss of the rocF gene caused a significant decrease in the virulence of X. oryzae pv. oryzae in the susceptible rice cultivar IR24. Bioinformatics analysis demonstrated that rocF encodes arginase. Quantitative real-time PCR and Western blot assays revealed that rocF expression was significantly induced by rice and arginine. The rocF deletion mutant strain showed elevated sensitivity to hydrogen peroxide, reduced extracellular polysaccharide (EPS) production, and reduced biofilm formation, all of which are important determinants for the full virulence of X. oryzae pv. oryzae, compared with the wild-type strain. Taken together, the results of this study revealed a mechanism by which a bacterial arginase is required for the full virulence of X. oryzae pv. oryzae on rice because of its contribution to tolerance to reactive oxygen species, EPS production, and biofilm formation.

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