scholarly journals Characterization of Organic Acid Metabolism and Expression of Related Genes During Fruit Development of Actinidia eriantha ‘Ganmi 6’

Plants ◽  
2020 ◽  
Vol 9 (3) ◽  
pp. 332
Author(s):  
Zhiqiang Jiang ◽  
Qing Huang ◽  
Dongfeng Jia ◽  
Min Zhong ◽  
Junjie Tao ◽  
...  
Keyword(s):  
Citric Acid ◽  
Organic Acids ◽  
Organic Acid ◽  
High Performance ◽  
Acid Metabolism ◽  
Citrate Synthase ◽  
Transcriptional Level ◽  
Post Translational Modification ◽  
Shikimate Dehydrogenase ◽  
Organic Acid Metabolism

Studies on organic acid metabolism have been mainly concentrated on the fruit, whereas, few have focused on the mechanism of high organic acids content in the fruit of Actinidia eriantha. Fruits of ‘Ganmi 6’ harvested at eleven developmental periods were used as materials. The components and content of organic acids were determined by high-performance liquid chromatography (HPLC) system, the activities of the related enzyme were detected, and gene expression levels were measured by quantitative real-time PCR (qRT-PCR). Components of ascorbic acid (AsA) and eight kinds of organic acids were detected. These results showed that quinic acid and citric acid were the main organic acids in the fruit of ‘Ganmi 6’. Correlation analysis showed that NADP-Quinate dehydrogenase (NADP-QDH), NADP-Shikimate dehydrogenase (NADP-SDH), and Cyt-Aconitase (Cyt-Aco) may be involved in regulating organic acids biosynthesis. Meanwhile, the SDH gene may play an important role in regulating the accumulation of citric acid. In this study, the activities of NADP-SDH, Mit-Aconitase (Mit-Aco), and NAD-Isocitrate dehydrogenase (NAD-IDH) were regulated by their corresponding genes at the transcriptional level. The activity of Citrate synthase (CS) may be affected by post-translational modification. Our results provided new insight into the characteristics of organic acid metabolism in the fruit of A. eriantha.

scholarly journals Identification of key gene networks controlling organic acid and sugar metabolism during watermelon fruit development by integrating metabolic phenotypes and gene expression profiles

2020 ◽  
Vol 7 (1) ◽  
Author(s):  
Muhammad Jawad Umer ◽  
Luqman Bin Safdar ◽  
Haileslassie Gebremeskel ◽  
Shengjie Zhao ◽  
Pingli Yuan ◽  
...  
Keyword(s):  
Organic Acids ◽  
Organic Acid ◽  
Gene Networks ◽  
Molecular Mechanisms ◽  
Acid Metabolism ◽  
Expression Profiles ◽  
Quantitative Polymerase Chain Reaction ◽  
Sugar Metabolism ◽  
Glucose Content ◽  
Organic Acid Metabolism

AbstractThe organoleptic qualities of watermelon fruit are defined by the sugar and organic acid contents, which undergo considerable variations during development and maturation. The molecular mechanisms underlying these variations remain unclear. In this study, we used transcriptome profiles to investigate the coexpression patterns of gene networks associated with sugar and organic acid metabolism. We identified 3 gene networks/modules containing 2443 genes highly correlated with sugars and organic acids. Within these modules, based on intramodular significance and Reverse Transcription Quantitative polymerase chain reaction (RT-qPCR), we identified 7 genes involved in the metabolism of sugars and organic acids. Among these genes, Cla97C01G000640, Cla97C05G087120 and Cla97C01G018840 (r2 = 0.83 with glucose content) were identified as sugar transporters (SWEET, EDR6 and STP) and Cla97C03G064990 (r2= 0.92 with sucrose content) was identified as a sucrose synthase from information available for other crops. Similarly, Cla97C07G128420, Cla97C03G068240 and Cla97C01G008870, having strong correlations with malic (r2 = 0.75) and citric acid (r2 = 0.85), were annotated as malate and citrate transporters (ALMT7, CS, and ICDH). The expression profiles of these 7 genes in diverse watermelon genotypes revealed consistent patterns of expression variation in various types of watermelon. These findings add significantly to our existing knowledge of sugar and organic acid metabolism in watermelon.

scholarly journals Changes in the Content of Organic Acids and Expression Analysis of Citric Acid Accumulation-Related Genes during Fruit Development of Yellow (Passiflora edulis f. flavicarpa) and Purple (Passiflora edulis f. edulis) Passion Fruits

2021 ◽  
Vol 22 (11) ◽  
pp. 5765
Author(s):  
Xiaoxue Zhang ◽  
Xiaoxia Wei ◽  
Muhammad Moaaz Ali ◽  
Hafiz Muhammad Rizwan ◽  
Binqi Li ◽  
...  
Keyword(s):  
Citric Acid ◽  
Organic Acids ◽  
Organic Acid ◽  
Fruit Development ◽  
Nutritive Value ◽  
Citrate Synthase ◽  
Passion Fruit ◽  
Mature Stage ◽  
Acid Biosynthesis ◽  
Passiflora Edulis

Organic acids are key components that determine the taste and flavor of fruits and play a vital role in maintaining fruit quality and nutritive value. In this study, the fruits of two cultivars of passion fruit Yellow (Passiflora edulis f. flavicarpa) and purple (Passiflora edulis f. edulis) were harvested at five different developmental stages (i.e., fruitlet, green, veraison, near-mature and mature stage) from an orchard located in subtropical region of Fujian Province, China. The contents of six organic acids were quantified using ultra-performance liquid chromatography (UPLC), activities of citric acid related enzymes were determined, and expression levels of genes involved in citric acid metabolism were measured by quantitative real-time PCR (qRT-PCR). The results revealed that citric acid was the predominant organic acid in both cultivars during fruit development. The highest citric acid contents were observed in both cultivars at green stage, which were reduced with fruit maturity. Correlation analysis showed that citrate synthase (CS), cytosolic aconitase (Cyt-ACO) and cytosolic isocitrate dehydrogenase (Cyt-IDH) may be involved in regulating citric acid biosynthesis. Meanwhile, the PeCS2, PeACO4, PeACO5 and PeIDH1 genes may play an important role in regulating the accumulation of citric acid. This study provides new insights for future elucidation of key mechanisms regulating organic acid biosynthesis in passion fruit.

The effects of biotin deficiency on organic acid metabolism: Increase in propionyl coenzyme A-related organic acids in biotin-deficient rats

Metabolism ◽  
1993 ◽  
Vol 42 (11) ◽  
pp. 1392-1397 ◽  
Author(s):  
Y-Y. Liu ◽  
Y. Shigematsu ◽  
A. Nakai ◽  
Y. Kikawa ◽  
M. Saito ◽  
...  
Keyword(s):  
Organic Acids ◽  
Organic Acid ◽  
Acid Metabolism ◽  
Coenzyme A ◽  
Biotin Deficiency ◽  
Organic Acid Metabolism

The Routine Investigation of Urinary Organic Acids in Selected Paediatric Patients: Results over a 2 1/2-Year Period

1984 ◽  
Vol 21 (1) ◽  
pp. 45-50 ◽  
Author(s):  
M J Bennett ◽  
Anne Green ◽  
R J Pollitt ◽  
E Worthy
Keyword(s):  
Metabolic Acidosis ◽  
Lactic Acidosis ◽  
Organic Acids ◽  
Organic Acid ◽  
Acid Metabolism ◽  
Diagnostic Yield ◽  
Inborn Errors ◽  
Routine Investigation ◽  
Urinary Organic Acids ◽  
Organic Acid Metabolism

Over a 2 1/2-year period 13 patients with inborn errors of organic acid metabolism, excluding undifferentiated lactic acidosis, have been diagnosed in our laboratories. The diagnostic yield in patients who had not previously been investigated by organic acid chromatography was 1 in 25, the majority of cases having presented with metabolic acidosis. A larger number of non-specific abnormalities were also detected. This type of investigation is beset with pitfalls and is extremely labour intensive.

Disorders of Organic Acid Metabolism

1988 ◽  
Vol 48 ◽  
pp. 78-78
Author(s):  
L. Dorland ◽  
M. Duran ◽  
J. B. C. de Klerk ◽  
F. J. Van Sprang ◽  
S. K. Wadman
Keyword(s):  
Organic Acid ◽  
Acid Metabolism ◽  
Organic Acid Metabolism
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