scholarly journals Supramolecular Cyclodextrin-Based Hydrogels for Controlled Gene Delivery

Polymers ◽  
2019 ◽  
Vol 11 (3) ◽  
pp. 514 ◽  
Author(s):  
Ana Rey-Rico ◽  
Magali Cucchiarini
Keyword(s):  
Gene Delivery ◽  
Delivery Systems ◽  
Controlled Delivery ◽  
Transfer Vectors ◽  
Spatial Presentation ◽  
Temporal And Spatial ◽  
Low Cytotoxicity ◽  
Dna Vectors ◽  
Powerful Strategy

Controlled delivery of gene transfer vectors is a powerful strategy to enhance the temporal and spatial presentation of therapeutic agents in a defined target. Hydrogels are adapted biomaterials for gene delivery capable of acting as a localized depot of genes while maintaining the long term local availability of DNA vectors at a specific location. Supramolecular hydrogels based on cyclodextrins (CDs) have attracted considerable attention as potential biomaterials in a broad range of drug delivery applications. Their unique characteristics of thixotropicity and low cytotoxicity due to their production under mild conditions make them potential candidates to form injectable delivery systems. This work aims to provide an overview of the use of CD-based polypseudorotaxane hydrogels as controlled gene delivery systems for different applications in regenerative medicine.

scholarly journals Hydrogel-Based Controlled Delivery Systems for Articular Cartilage Repair

2016 ◽  
Vol 2016 ◽  
pp. 1-12 ◽  
Author(s):  
Ana Rey-Rico ◽  
Henning Madry ◽  
Magali Cucchiarini
Keyword(s):  
Articular Cartilage ◽  
Cartilage Repair ◽  
Inflammatory Responses ◽  
Delivery Systems ◽  
Controlled Delivery ◽  
Bioactive Molecules ◽  
Therapeutic Action ◽  
Articular Cartilage Repair ◽  
Spatial Presentation ◽  
Temporal And Spatial

Delivery of bioactive factors is a very valuable strategy for articular cartilage repair. Nevertheless, the direct supply of such biomolecules is limited by several factors including rapid degradation, the need for supraphysiological doses, the occurrence of immune and inflammatory responses, and the possibility of dissemination to nontarget sites that may impair their therapeutic action and raise undesired effects. The use of controlled delivery systems has the potential of overcoming these hurdles by promoting the temporal and spatial presentation of such factors in a defined target. Hydrogels are promising materials to develop delivery systems for cartilage repair as they can be easily loaded with bioactive molecules controlling their release only where required. This review exposes the most recent technologies on the design of hydrogels as controlled delivery platforms of bioactive molecules for cartilage repair.

Adeno-Associated Viral Vectors for Gene Transfer and Gene Therapy

1999 ◽  
Vol 380 (6) ◽  
Author(s):  
H. Büeler
Keyword(s):  
Gene Expression ◽  
Gene Therapy ◽  
Gene Transfer ◽  
Gene Delivery ◽  
Viral Vectors ◽  
Delivery Systems ◽  
Adeno Associated Virus ◽  
Viral Genes ◽  
Virus Recombinant

AbstractAdeno-associated virus (AAV) is a defective, non-pathogenic human parvovirus that depends for growth on coinfection with a helper adenovirus or herpes virus. Recombinant adeno-associated viruses (rAAVs) have attracted considerable interest as vectors for gene therapy. In contrast to other gene delivery systems, rAAVs lack all viral genes and show long-term gene expression

scholarly journals Endocrine regulation of long-term enhancement of spermiation in meagre (Argyrosomus regius) with GnRHa controlled-delivery systems

2020 ◽  
Vol 297 ◽  
pp. 113549 ◽  
Author(s):  
Ioannis Fakriadis ◽  
Eugenio Maria Zanatta ◽  
Renata Pontes Dos Santos Fleck ◽  
Daybet Lorena Sena Mateo ◽  
Maria Papadaki ◽  
...  
Keyword(s):  
Delivery Systems ◽  
Controlled Delivery ◽  
Endocrine Regulation ◽  
Argyrosomus Regius

scholarly journals Hydrogel-Based Localized Nonviral Gene Delivery in Regenerative Medicine Approaches—An Overview

Pharmaceutics ◽  
2020 ◽  
Vol 12 (8) ◽  
pp. 752 ◽  
Author(s):  
Natalia Carballo-Pedrares ◽  
Isaac Fuentes-Boquete ◽  
Silvia Díaz-Prado ◽  
Ana Rey-Rico
Keyword(s):  
Wound Healing ◽  
Gene Delivery ◽  
Regenerative Medicine ◽  
Controlled Delivery ◽  
Nonviral Gene Delivery ◽  
Specific Gene ◽  
Target Tissue ◽  
Avant Garde ◽  
Neural Tissues ◽  
Powerful Strategy

Hydrogel-based nonviral gene delivery constitutes a powerful strategy in various regenerative medicine scenarios, as those concerning the treatment of musculoskeletal, cardiovascular, or neural tissues disorders as well as wound healing. By a minimally invasive administration, these systems can provide a spatially and temporarily defined supply of specific gene sequences into the target tissue cells that are overexpressing or silencing the original gene, which can promote natural repairing mechanisms to achieve the desired effect. In the present work, we provide an overview of the most avant-garde approaches using various hydrogels systems for controlled delivery of therapeutic nucleic acid molecules in different regenerative medicine approaches.

Use of Lipids, Polymers, and Peptides for Drug Delivery and Targeting to Cancer Cells or Specific Organs

2021 ◽  
pp. 276-289
Author(s):  
Sampan Attri ◽  
Shruti Sharma ◽  
Ujjawal Sharma ◽  
Manjita Srivastava ◽  
Subash C. Sonkar ◽  
...  
Keyword(s):  
Drug Delivery ◽  
Cancer Therapy ◽  
Drug Delivery Systems ◽  
Structural Characteristics ◽  
Delivery Systems ◽  
Chemotherapeutic Agents ◽  
Delivery Methods ◽  
Practical Applications ◽  
Low Cytotoxicity

Cancer has been the most deleterious disease in recent times, and unfortunately its spread is increasing. Systemic treatment with chemotherapeutics remains the conventional way of treating many cancers, despite the serious damage long-term chemotherapy can cause in healthy tissues. Many therapeutic strategies have achieved popular practical applications, but drug delivery systems still face challenges associated with safety, and this has led to the development of safer drug delivery methods composed of biocompatible substances. In this respect, lipid-, polymer-, and peptide-based drug delivery systems have been proposed as safer candidates for cancer therapy. These delivery methods are expected to as biodegradable systems with low cytotoxicity for cancer therapy. Therefore, in this chapter, the authors discuss use of lipids, polymers, and peptides as delivery vehicles for chemotherapeutic agents and their structural characteristics.

Enhanced Stability and Controlled Delivery of MOF Encapsulated Vaccines and Their Immunogenic Response In Vivo

2018 ◽  
Author(s):  
Michael Luzuriaga ◽  
Raymond P. Welch ◽  
Madushani Dharmawardana ◽  
Candace Benjamin ◽  
Shaobo Li ◽  
...  
Keyword(s):  
Viral Vector ◽  
Controlled Delivery ◽  
Conformational Structure ◽  
Spectroscopy Analysis ◽  
Zeolitic Imidazolate Framework ◽  
Structural Conformation ◽  
Depth Study ◽  
Viral Nanoparticle

<div><div><div><p>Vaccines have an innate tendency to lose their structural conformation upon environmental and chemical stressors. A loss in conformation reduces the therapeutic ability to prevent the spread of a pathogen. Herein, we report an in-depth study of zeolitic imidazolate framework-8 (ZIF-8) and its ability to provide protection for a model viral vector against dena- turing conditions. The immunoassay and spectroscopy analysis together demonstrate enhanced thermal and chemical stability to the conformational structure of the encapsulated viral nanoparticle. The long-term biological activity of this virus-ZIF composite was investigated in animal models to further elucidate the integrity of the encapsulated virus, the bio-safety, and immunogenicity of the overall composite. Additionally, histological analysis found no observable tissue damage in the skin or vital organs in mice, following multiple subcutaneous administrations. This study shows that ZIF-based protein composites are strong candidates for improved preservation of proteinaceous drugs, are biocompatible, and capable of controlling the release and adsorption of drugs in vivo.</p></div></div></div>

Recent advances in the analysis full/empty capsid ratio and genome integrity of adeno-associated virus (AAV) gene delivery vectors

2020 ◽  
Vol 20 ◽  
Author(s):  
L. Hajba ◽  
A. Guttman
Keyword(s):  
Gene Delivery ◽  
High Efficiency ◽  
Analytical Techniques ◽  
Viral Gene ◽  
Adeno Associated Virus ◽  
Gene Delivery Vectors ◽  
Empty Capsid ◽  
Purification Methods ◽  
Viral Gene Delivery

: Adeno-associated virus (AAV) is one of the most promising viral gene delivery vectors with long-term gene expression and disease correction featuring high efficiency and excellent safety in human clinical trials. During the production of AAV vectors,there are several quality control (QC)parameters that should be rigorously monitored to comply with clini-cal safety and efficacy. This review gives a short summary of the most frequently used AVV production and purification methods,focusing on the analytical techniques applied to determine the full/empty capsid ratio and the integrity of the encapsidated therapeutic DNA of the products.

Star Shaped Poly(ethylene glycols) Yield Biocompatible Gene Delivery Systems

2015 ◽  
Vol 2 (4) ◽  
pp. 182-195 ◽  
Author(s):  
Jayanant Iemsam-Arng ◽  
Xiao Kong ◽  
Andreas G. Schatzlein ◽  
Ijeoma F. Uchegbu
Keyword(s):  
Gene Delivery ◽  
Delivery Systems ◽  
Ethylene Glycols ◽  
Poly Ethylene
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