scholarly journals Differential Biodegradation Kinetics of Collagen Membranes for Bone Regeneration

Polymers ◽  
2020 ◽  
Vol 12 (6) ◽  
pp. 1290
Author(s):  
Manuel Toledano ◽  
Samara Asady ◽  
Manuel Toledano-Osorio ◽  
Franklin García-Godoy ◽  
María-Angeles Serrera-Figallo ◽  
...  
Keyword(s):  
Bone Regeneration ◽  
Hydrolytic Degradation ◽  
Phosphate Buffer Solution ◽  
Buffer Solution ◽  
Barrier Membranes ◽  
Time Points ◽  
Equine Pericardium ◽  
Collagen Membranes ◽  
Kinetics Of ◽  
Digital Caliper

Native collagen-based membranes are used to guide bone regeneration; but due to their rapid biodegradation, this treatment is often unpredictable. The purpose of this study was to investigate the biodegradability of natural collagen membranes. Three non-cross-linked resorbable collagen barrier membranes were tested: Derma Fina (porcine dermis), Evolution Standard (equine pericardium) and Duo-Teck (equine lyophilized collagen felt). 10 × 10 mm2 pieces of membranes were submitted to three different degradation procedures: (1) hydrolytic degradation in phosphate buffer solution, (2) enzyme resistance, using a 0.25% porcine trypsin solution, and (3) bacterial (Clostridium histolyticum) collagenase resistance test. Weight measurements were performed with an analytic microbalance. Thickness was measured with a digital caliper. Membranes were analyzed at different time-points, up to 21 d of immersion. A stereomicroscope was used to obtain membranes’ images. ANOVA and Student Newman Keuls were used for mean comparisons (p < 0.05), except when analyzing differences between time-points within the same membrane and solution where pair-wise comparisons were applied (p < 0.001). Derma Fina attained the highest resistance to all degradation challenges. Duo-Teck was the most susceptible membrane to degradation, complete degradation occurred as soon as 8 h. The bacterial collagenase solution performed as the most aggressive test as all membranes presented 100% degradation before 21 d.

scholarly journals Synthesis, Characterization, and Biodegradation Studies of Poly(1,4-cyclohexanedimethylene-adipate-carbonate)s

2014 ◽  
Vol 2014 ◽  
pp. 1-11 ◽  
Author(s):  
Ajay S. Chandure ◽  
Ganesh S. Bhusari ◽  
Suresh S. Umare
Keyword(s):  
Weight Loss ◽  
Enzymatic Degradation ◽  
Hydrolytic Degradation ◽  
Phosphate Buffer Solution ◽  
Film Surface ◽  
Solution Viscosity ◽  
Buffer Solution ◽  
Sem Images ◽  
Titanium Butoxide ◽  
Soil Burial

Aliphatic/alicyclic poly(1,4-cyclohexanedimethylene-adipate-carbonate)s (PCACs) were synthesized by a transesterification from 1,4-cyclohexamethylendimethanol (1,4-CHDM), adipic acid (AA), diethyl carbonate (DEC), and titanium butoxide Ti(OBu)4 as a transesterification catalyst. The synthesized PCACs were characterized by the Fourier transform infrared (FTIR), X-ray diffraction analysis (XRD), solubility, solution viscosity, gel permeation chromatography (GPC), differential scanning calorimetry (DSC), thermogravimetric analysis (TGA), and scanning electron microscope (SEM) for their structural, physical, thermal, and morphological investigation. The structure of synthesized PCACs was confirmed by FTIR. All TGA curves of PCACs shows 10% weight loss above 270°C, and they reveal good thermal stability. Biodegradability of PCACs was investigated by hydrolytic degradation at (pH 7.2 and 11.5), enzymatic degradation using Rhizopus delemar lips at 37°C in phosphate buffer solution (PBS), and soil burial degradation at 30°C. The hydrolytic degradation shows the greater rate of weight loss in PBS at pH-11.5 than pH-7.2. The hydrolytic and soil burial degradation shows faster rate of weight loss as compared to enzymatic degradation. Biodegradation rate of PCACs follows the order: PCAC-20 > PCAC-40 > PCAC-60. SEM images show that degradation occurred all over the film surface, creating holes and cracks. These biodegradable PCACs may be able to replace conventional polymer in the fabrication of packaging film in near future.

scholarly journals Selection of Collagen Membranes for Bone Regeneration: A Literature Review

Materials ◽  
2020 ◽  
Vol 13 (3) ◽  
pp. 786 ◽  
Author(s):  
Luca Sbricoli ◽  
Riccardo Guazzo ◽  
Marco Annunziata ◽  
Luca Gobbato ◽  
Eriberto Bressan ◽  
...  
Keyword(s):  
Bone Regeneration ◽  
Clinical Performance ◽  
Guided Bone Regeneration ◽  
Treatment Modalities ◽  
Barrier Membranes ◽  
Collagen Membranes ◽  
Collagen Versus ◽  
The Right ◽  
Grafting Materials ◽  
Selection Of

Several treatment modalities have been proposed to regenerate bone, including guided bone regeneration (GBR) where barrier membranes play an important role by isolating soft tissue and allowing bone to grow. Not all membranes biologically behave the same way, as they differ from their origin and structure, with reflections on their mechanical properties and on their clinical performance. Collagen membranes have been widely used in medicine and dentistry, because of their high biocompatibility and capability of promoting wound healing. Recently, collagen membranes have been applied in guided bone regeneration with comparable outcomes to non-resorbable membranes. Aim of this work is to provide a review on the main features, application, outcomes, and clinical employment of the different types of collagen membranes. Comparisons with non-resorbable membranes are clarified, characteristics of cross-linked collagen versus native collagen, use of different grafting materials and need for membrane fixation are explored in order to gain awareness of the indications and limits and to be able to choose the right membrane required by the clinical condition.

scholarly journals DETECTION OF HYDROPEROXIDES IN SOLUTIONS OF PHOTOOXIDIZED PSORALEN

2019 ◽  
Vol 14 (1) ◽  
pp. 32-38
Author(s):  
V. V. Skarga ◽  
E. V. Nevezhin ◽  
A. А. Matrosov ◽  
V. V. Negrebetsky ◽  
M. V. Malakhov
Keyword(s):  
Quantitative Analysis ◽  
Phosphate Buffer ◽  
Biological Effects ◽  
Xylenol Orange ◽  
Phosphate Buffer Solution ◽  
Buffer Solution ◽  
Uva Irradiation ◽  
Fox Assay ◽  
Kinetics Of ◽  
Ferrous Oxidation

Photooxidized psoralen solutions possess a variety of biological effects, which implementation mechanism may presumably involve hydroperoxides. Here, the hydroperoxide content in photooxidized psoralen solutions was assessed using photometric FOX assay (from Ferrous Oxidation + Xylenol Orange). FOX reagent with 10× content of Xylenol Orange, modified for quantitative analysis of up to 50 μM of hydroperoxides in aqueous phase was used in experiments. During photooxidation of 0.1 mM psoralen in phosphate buffer solution, hydroperoxide production increases with dose of UVA irradiation (~2.5 μM eq. of H2O2 for dose of 252 kJ/m2 and ~11 μM eq. of H2O2 for dose of 1512 kJ/m2) and reaches ~16.5 μM eq. of H2O2 at the highest dose investigated (3024 kJ/m2). A comparison of kinetics of psoralen photolysis and hydroperoxide generation allows us to suggest that generation of hydroperoxide results from the secondary photochemical processes involving psoralen photoproducts, presumably from photoinduced autooxidation of aldehydic photoproducts of psoralen.

In Vitro Biodegradability of Poly(lactic Acid)/Hydroxyapatite Biocomposites Prepared by Solvent-Blending Technique

2012 ◽  
Vol 626 ◽  
pp. 631-635 ◽  
Author(s):  
Mujtahid Kaavessina ◽  
Fitriani Khanifatun ◽  
Imtiaz Ali ◽  
Saeed M. Alzahrani
Keyword(s):  
Lactic Acid ◽  
Hydrolytic Degradation ◽  
Phosphate Buffer Solution ◽  
Weight Fraction ◽  
Poly Lactic Acid ◽  
Buffer Solution ◽  
Before And After ◽  
Micro Holes ◽  
Thermal Stability Of

Poly (lactic acid) was solvent-blended and formed as thin ribbons with different weight fraction of hydroxyapatite, namely 5, 10 and 20wt%. In-vitro biodegradability of biocomposites was performed in phosphate buffer solution (PBS) at 37°C. The presence of hydroxyapatite tended to increase biodegradability of poly (lactic acid) in its biocomposites. Thermal stability of biocomposites was always higher than that neat poly (lactic acid) either before and after hydrolytic degradation tests. After biodegradation tests, some micro-holes and cracks were appeared in the surface morphology of biocomposites as well as the increasing crystallinity occurred.

scholarly journals Biological Compatibility of a Polylactic Acid Composite Reinforced with Natural Chitosan Obtained from Shrimp Waste

Materials ◽  
2018 ◽  
Vol 11 (8) ◽  
pp. 1465 ◽  
Author(s):  
Yaret Torres-Hernández ◽  
Gloria Ortega-Díaz ◽  
Lucía Téllez-Jurado ◽  
Nayeli Castrejón-Jiménez ◽  
Alejandro Altamirano-Torres ◽  
...  
Keyword(s):  
Cell Viability ◽  
Polylactic Acid ◽  
Low Cost ◽  
Hydrolytic Degradation ◽  
Phosphate Buffer Solution ◽  
Morphological Characteristics ◽  
Buffer Solution ◽  
Composite Surface ◽  
Biological Compatibility

The aim of this work is to evaluate the effect of chitosan content (1, 3 and 5 wt %) dispersed in polylactic acid (PLA) on the structure and properties of composites. Also, the hydrolytic degradation, and the cell viability and adhesion of human MG-63 osteoblasts are analyzed to determine the composites’ suitability for use in tissue engineering. For the manufacture of the materials, natural chitosan was extracted chemically from shrimp exoskeleton. The composites were fabricated by extrusion, because it is a low-cost process, it is reproducible, and it does not compromise the biocompatibility of the materials. FT-IR and XRD show that the chitosan does not change the polymer structure, and interactions between the composite components are discarded. In vitro degradation tests show that the composites do not induce significant pH changes in phosphate buffer solution due to their low susceptibility to hydrolytic degradation. The adhesion and morphological characteristics of the osteoblasts are evaluated using confocal microscopy and scanning electron microscopy. The cell viability is determined by the MTT assay. Osteoblasts adhesion is observed on the surface of PLA and composites. A higher amount of chitosan, higher number of cells with osteoblastic morphology, and mineralized nodules are observed on the composite surface. The highest metabolic activity is evidenced at 21 days. The results suggest that the Polylactic acid/chitosan composites are potentially suitable for use as a biomaterial.

scholarly journals In Vitro Biodegradation Pattern of Collagen Matrices for Soft Tissue Augmentation

Polymers ◽  
2021 ◽  
Vol 13 (16) ◽  
pp. 2633
Author(s):  
Cristina Vallecillo ◽  
Manuel Toledano-Osorio ◽  
Marta Vallecillo-Rivas ◽  
Manuel Toledano ◽  
Raquel Osorio
Keyword(s):  
Soft Tissue ◽  
Hydrolytic Degradation ◽  
Phosphate Buffer Solution ◽  
Soft Tissue Augmentation ◽  
Buffer Solution ◽  
Collagen Matrices ◽  
Bacterial Collagenase ◽  
Tissue Augmentation ◽  
Tissue Grafts

Collagen matrices have become a great alternative to the use of connective tissue grafts for soft tissue augmentation procedures. One of the main problems with these matrices is their volume instability and rapid degradation. This study has been designed with the objective of examining the degradation of three matrices over time. For this purpose, pieces of 10 × 10 mm2 of Fibro-Gide, Mucograft and Mucoderm were submitted to three different degradation tests—(1) hydrolytic degradation in phosphate buffer solution (PBS); (2) enzyme resistance, using a 0.25% porcine trypsin solution; and (3) bacterial collagenase resistance (Clostridium histolyticum)—over different immersion periods of up to 50 days. Weight measurements were performed with an analytic microbalance. Thickness was measured with a digital caliper. A stereomicroscope was used to obtain the matrices’ images. ANOVA and Student–Newman–Keuls tests were used for mean comparisons (p < 0.05), except when analyzing differences between time-points within the same matrix and solution, where pair-wise comparisons were applied (p < 0.001). Fibro-Gide attained the highest resistance to all degradation challenges. The bacterial collagenase solution was shown to constitute the most aggressive test as all matrices presented 100% degradation before 14 days of storage.

Adsorption and release kinetics of growth factors on barrier membranes for guided tissue/bone regeneration: A systematic review

2019 ◽  
Vol 100 ◽  
pp. 57-68 ◽  
Author(s):  
Jordi Caballé-Serrano ◽  
Yusra Abdeslam-Mohamed ◽  
Antonio Munar-Frau ◽  
Masako Fujioka-Kobayashi ◽  
Federico Hernández-Alfaro ◽  
...  
Keyword(s):  
Systematic Review ◽  
Growth Factors ◽  
Bone Regeneration ◽  
Release Kinetics ◽  
Barrier Membranes ◽  
Adsorption And Release ◽  
Kinetics Of

scholarly journals The Collagen Origin Influences the Degradation Kinetics of Guided Bone Regeneration Membranes

Polymers ◽  
2021 ◽  
Vol 13 (17) ◽  
pp. 3007
Author(s):  
Marta Vallecillo-Rivas ◽  
Manuel Toledano-Osorio ◽  
Cristina Vallecillo ◽  
Manuel Toledano ◽  
Raquel Osorio
Keyword(s):  
Bone Regeneration ◽  
Degradation Kinetics ◽  
Hydrolytic Degradation ◽  
Guided Bone Regeneration ◽  
Collagen Membrane ◽  
Bacterial Collagenase ◽  
Degradation Tests ◽  
Collagen Membranes ◽  
Different Origins ◽  
Porcine Pericardium

Collagen membranes are currently the most widely used membranes for guided bone regeneration; however, their rapid degradation kinetics means that the barrier function may not remain for enough time to permit tissue regeneration to happen. The origin of collagen may have an important effect on the resistance to degradation. The aim of this study was to investigate the biodegradation pattern of five collagen membranes from different origins: Biocollagen, Heart, Evolution X-fine, CopiOs and Parasorb Resodont. Membranes samples were submitted to different degradation tests: (1) hydrolytic degradation in phosphate buffer saline solution, (2) bacterial collagenase from Clostridium histolyticum solution, and (3) enzyme resistance using a 0.25% porcine trypsin solution. Immersion periods from 1 up to 50 days were performed. At each time point, thickness and weight measurements were performed with a digital caliper and an analytic microbalance, respectively. ANOVA and Student–Newman–Keuls tests were used for comparisons (p < 0.05). Differences between time-points within the same membranes and solutions were assessed by pair-wise comparisons (p < 0.001). The Evolution X-fine collagen membrane from porcine pericardium attained the highest resistance to all of the degradation tests. Biocollagen and Parasorb Resodont, both from equine origin, experienced the greatest degradation when immersed in PBS, trypsin and C. histolyticum during challenge tests. The bacterial collagenase solution was shown to be the most aggressive testing method.

scholarly journals Study of the dissolution kinetics of drugs in solid dosage form with lisinopril and atorvastatin and intestinal permeability to assess their equivalence in vitro

Pharmacia ◽  
2022 ◽  
Vol 69 (1) ◽  
pp. 61-67
Author(s):  
Nataliia Shulyak ◽  
Kateryna Liushuk ◽  
Oksana Semeniuk ◽  
Nadiya Yarema ◽  
Tetyana Uglyar ◽  
...  
Keyword(s):  
Intestinal Permeability ◽  
Dosage Form ◽  
Phosphate Buffer Solution ◽  
Dissolution Kinetics ◽  
Acetate Buffer Solution ◽  
Buffer Solution ◽  
Solid Dosage Form ◽  
Solid Dosage ◽  
Kinetics Of

Atorvastatin and lisinopril are a successful combination for the treatment of patients with chronic heart failure and hypertension. Study of the dissolution kinetics of drugs in solid dosage form with lisinopril and atorvastatin and intestinal permeability to assess their equivalence in vitro were described. In medium with hydrochloric acid pH 1.2, in the medium of acetate buffer solution with a pH of 4.5 and in the medium phosphate buffer solution with a pH of 6.8 for 15 min more than 85% of the active substance passes into solution, hence the dissolution profiles these drugs in these environments are similar, and the drugs in them are “very quickly soluble”. Among the in vitro models that make it possible to assess the degree of absorption of API, the most widely used culture of adenocarcinoma cells of the colon – Caco-2. The development of the analytical methodology and its validation is the final stage of both the dissolution study and the Caco-2 test, as well as the biowaver procedure. It plays the most important role in the reliability of the results for all the above procedures and tests. To study permeability, method LC-MS/MS was developed. According to the obtained results, atorvastatin and lisinopril showed low permeability. The values ​​of recovery of transport of test and control substances through the monolayer of cells of the Caco-2 line indicate that the results of the experiment can be considered reliable. The equivalence of the drugs “Lisinopril”, tablets of 10 mg and “Atorvastatin”, tablets of 10 mg, belongs to class III BCS proven by in vitro studies.

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