scholarly journals Improvement in the Microbial Resistance of Resin-Based Dental Sealant by Sulfobetaine Methacrylate Incorporation

Polymers ◽  
2020 ◽  
Vol 12 (8) ◽  
pp. 1716 ◽  
Author(s):  
Myung-Jin Lee ◽  
Utkarsh Mangal ◽  
Se-Jin Kim ◽  
Yeo-Phil Yoon ◽  
Eun-So Ahn ◽  
...  
Keyword(s):  
Dental Caries ◽  
Flexural Strength ◽  
Research Area ◽  
Cell Cytotoxicity ◽  
Water Contact ◽  
Bacterial Viability ◽  
Cariogenic Bacteria ◽  
Dental Sealant ◽  
L929 Fibroblast ◽  
Protein Adhesion

Prevention of dental caries is a key research area, and improvement of the pit and fissure sealants used for caries prevention has been of particular interest. This report describes results of incorporating a zwitterion, sulfobetaine methacrylate (SB), into photo-polymerized resin-based sealants to enhance resistance to cariogenic bacteria and protein adhesion. Varying amounts (1.5–5 wt%) of SB were incorporated into a resin-based sealant, and the flexural strength, wettability, depth of cure, protein adhesion, bacterial viability, and cell cytotoxicity of the resultant sealants were evaluated. The flexural strength decreased with the increasing SB content, but this decrease was statistically significant only for sealants containing ≥3 wt% SB. Incorporating a zwitterion led to a significant reduction in the water contact angle and protein adhesion. The colony-forming unit count showed a significant reduction in the bacterial viability of S. mutans, which was confirmed with microscopic imaging. Moreover, cell cytotoxicity analysis of SB-modified sealants using an L929 fibroblast showed a cytotoxicity comparable to that of an unmodified control, suggesting no adverse effects on the cellular metabolism upon SB introduction. Hence, we conclude that the addition of 1.5–3 wt% SB can significantly enhance the inherent ability of sealants to resist S. mutans adhesion and prevent dental caries.

Formation of Cariogenic Bacterial Biofilm on Vanillin-Incorporated Resin-Based Dental Sealant

2021 ◽  
Vol 889 ◽  
pp. 107-111
Author(s):  
Boonyanit Thaweboon ◽  
Sroisiri Thaweboon
Keyword(s):  
Dental Caries ◽  
Biofilm Formation ◽  
Antimicrobial Agents ◽  
Antimicrobial Property ◽  
Bacterial Biofilm ◽  
Cell Counting ◽  
Cariogenic Bacteria ◽  
Tooth Structure ◽  
Dental Sealants ◽  
Dental Sealant

Tooth decay or dental caries is an important oral health problem involving people of all age groups. The disease is the outcome of the demineralize process in which aciduric and acidogenic bacteria in a biofilm decompose tooth structure. Dental sealant, a resin material, which is applied on the occlusal pit and fissure surfaces of the teeth as a protective layer has been commonly used to prevent dental caries. However, the microbial effect on food residue is found to be a major cause of microleakage of sealant and secondary caries. Several types of antimicrobial agents were introduced to increase the caries preventive effect of dental sealants. Vanillin, the main component of flavoring agent vanilla, has been found to have antimicrobial property against Gram-positive and Gram-negative bacteria. The objective of this study was to investigate the antimicrobial effect of vanillin-incorporated dental sealant against biofilm formation of cariogenic bacteria. Dental sealant resin samples (Clinpro; 3M ESPE, USA) were prepared in 96-well plate in accordance with the amount of vanillin adding (0%, 0.5%, 1% and 5% vanillin). The cariogenic bacterial suspensions of Streptococcus mutans ATCC 25175 and Lactobacillus casei ATCC 334 were added to saliva-coated samples and incubated at 37°C in 5% CO2 atmosphere for 48 h to allow the biofilm formation. The quantity of vital biofilm was determined by WST Microbial Cell Counting Kit (Dojindo Molecular Technologies, USA) at 460 nm. One-way ANOVA and Tukey’s test were applied to the statistical analysis. A significant inhibitory effect against L. casei biofilm was observed in all vanillin incorporated samples (0.5%, 1% and 5% vanillin) compared with samples without vanillin. The percentage of biofilm reduction was 32-39%. For S. mutans, the suppressive effect was noticed only at >1% vanillin with 18-25% biofilm reduction. In conclusion, the incorporation of vanillin to dental sealants could decrease biofilm formation of cariogenic bacteria (S. mutans and L. casei). The use of dental sealants containing vanillin could be a promising measure to prevent dental caries due to their antibacterial biofilm formation property.

scholarly journals Lactobacillus reuteri BM53-1 Produces a Compound That Inhibits Sticky Glucan Synthesis by Streptococcus mutans

2021 ◽  
Vol 9 (7) ◽  
pp. 1390
Author(s):  
Masafumi Noda ◽  
Naho Sugihara ◽  
Yoshimi Sugimoto ◽  
Ikue Hayashi ◽  
Sachiko Sugimoto ◽  
...  
Keyword(s):  
Antibacterial Activity ◽  
Lactic Acid ◽  
Dental Caries ◽  
Lactobacillus Reuteri ◽  
Early Stage ◽  
Pcr Analysis ◽  
Cariogenic Bacteria ◽  
Qrt Pcr ◽  
Actinidia Polygama ◽  
Glucan Synthesis

Cariogenic bacteria, such as Streptococcus (S.) mutans and S. sobrinus, produce insoluble and sticky glucans as a biofilm material. The present study demonstrates that a lactic acid bacterium (LAB) named BM53-1 produces a substance that inhibits the sticky glucan synthesis. The BM53-1 strain was isolated from a flower of Actinidia polygama and identified as Lactobacillus reuteri. The substance that inhibits sticky glucan synthesis does not exhibit antibacterial activity against S. mutans. The cariogenic S. mutans produces glucans under the control of three glucosyltransferase (GTF) enzymes, named GtfB, GtfC, and GtfD. Although GtfB and GtfC produce insoluble glucans, GtfD forms soluble glucans. Through quantitative reverse-transcriptional (qRT)-PCR analysis, it was revealed that the BM53-1-derived glucan-production inhibitor (GI) enhances the transcriptions of gtfB and gtfC genes 2- to 7-fold at the early stage of cultivation. However, that of gtfD was not enhanced in the presence of the GI, indicating that the glucan stickiness produced by S. mutans was significantly weaker in the presence of the GI. Our result demonstrates that Lb. reuteri BM53-1 is useful to prevent dental caries.

scholarly journals Novel lactotransferrin-derived synthetic peptides suppress cariogenic bacteria in vitro and arrest dental caries in vivo

2021 ◽  
Vol 13 (1) ◽  
pp. 1943999
Author(s):  
Junyuan Luo ◽  
Zening Feng ◽  
Wentao Jiang ◽  
Xuelian Jiang ◽  
Yue Chen ◽  
...  
Keyword(s):  
Dental Caries ◽  
Synthetic Peptides ◽  
Cariogenic Bacteria

scholarly journals P878 Are cariogenic bacteria the major risk factor for dental caries in patients with ulcerative colitis?

2018 ◽  
Vol 12 (supplement_1) ◽  
pp. S561-S562
Author(s):  
E Rodrigues ◽  
N Laranjeira ◽  
G Nunes ◽  
R Barosa ◽  
M Patita ◽  
...  
Keyword(s):  
Ulcerative Colitis ◽  
Risk Factor ◽  
Dental Caries ◽  
Major Risk Factor ◽  
Cariogenic Bacteria

scholarly journals Potentials of Mouthwashes in Disinfecting Cariogenic Bacteria and Biofilms Leading to Inhibition of Caries

2012 ◽  
Vol 6 (1) ◽  
pp. 23-30 ◽  
Author(s):  
Takehiro Oyanagi ◽  
Junji Tagami ◽  
Khairul Matin
Keyword(s):  
Ex Vivo ◽  
Secondary Caries ◽  
Bacterial Viability ◽  
Cariogenic Bacteria ◽  
Benzethonium Chloride ◽  
Large Numbers ◽  
Carious Lesions ◽  
Colony Forming Units ◽  
Caries Model ◽  
Phosphate Buffered Saline

Objectives:The aim of this study was to compare the effects of certain commercially available mouthwashes on cariogenic bacteria and biofilms, following the acquisition of inhibition potentials of caries.Materials and Methods:Mouthwashes containing I) chlorhexidine gluconate (CHG; 0.0005% w/v), II) benzethonium chloride (BTC; 0.01% w/v), III) an essential oil (Listerine), and IV) povidone-iodine (PVP-I; 0.035% w/v) were tested on planktonic cariogenic bacteria, biofilms, and an ex vivo caries model. Bacterial aliquots were inoculated with each solution separately and vortexed for 10 seconds at room temperature. Bacterial viability was subsequently investigated by fluorescence microscopy (FM) after staining with a BacLight viability kit and the number of colony-forming units (CFUs) was counted. Similarly, mouthwash solutions were applied to artificial cariogenic biofilms, and bacterial viability of the biofilms was investigated as stated above. Inhibition potentials of two selected mouthwashes of carious lesions were investigated using biofilm-induced caries and a secondary caries model. In all steps, a phosphate-buffered saline (PBS) solution was included as a control.Results:Planktonic cariogenic bacteria and bacteria embedded in biofilms were killed in remarkably large numbers with Listerine and PVP-I treatment compared to PBS and other gargles. CFU counts also showed significant reduction after treatment with Listerine and PVP-I compared to other solutions (P<0.05). Listerine also displayed significant (P<0.05) inhibition effects in preventing the progression of demineralization.Conclusion:Bactericidal potencies of the mouthwashes varied significantly, suggesting that mouthwashes like Listerine can be useful for the prevention of caries and secondary caries.

scholarly journals In VitroAntibacterial Activity of Pomegranate Juice and Peel Extracts on Cariogenic Bacteria

2017 ◽  
Vol 2017 ◽  
pp. 1-7 ◽  
Author(s):  
Gianmaria Fabrizio Ferrazzano ◽  
Elisa Scioscia ◽  
Daniela Sateriale ◽  
Gabiria Pastore ◽  
Roberta Colicchio ◽  
...  
Keyword(s):  
Dental Caries ◽  
Clinical Isolate ◽  
Inhibitory Activity ◽  
Punica Granatum ◽  
Pomegranate Juice ◽  
Tooth Decay ◽  
Growth And Survival ◽  
Cariogenic Bacteria ◽  
Etiologic Agents

Aim. To evaluate the antimicrobial activity of hydroalcoholic extracts of pomegranate (Punica granatumL.) peel and juice, against the microorganisms considered the main etiologic agents of dental caries.Methods. The values of the minimum inhibitory concentration (MIC) and the minimum bactericidal concentration (MBC) were determined againstStreptococcus mutansClarke ATCC® 25175™ strain andRothia dentocariosaclinical isolate.Results. Peel extracts inhibit effectively the growth and survival ofS. mutansATCC 25175 strain andR. dentocariosaclinical isolate with MIC and MBC values of 10 μg/μl and 15 μg/μl, respectively. Furthermore, the pomegranate juice extract showed high inhibitory activity againstS. mutansATCC 25175 strain with a MIC value of 25 μg/μl and a MBC value of 40 μg/μl, whereas, againstR. dentocariosa, it has displayed a moderate inhibitory activity, with MIC and MBC values of 20 μg/μl and 140 μg/μl, respectively.Conclusions.In vitromicrobiological tests demonstrate that the hydroalcoholic extracts of pomegranate juice and peel are able to contrast the main cariogenic bacteria involved in tooth decay. Although being preliminary data, our results suggest that pomegranate polyphenolic compounds could represent a good adjuvant for the prevention and treatment of dental caries.

scholarly journals The estimation of the viable count of mutans streptococcus in waterpipe smokers and cigarette smokers

2021 ◽  
Vol 33 (3) ◽  
pp. 23-29
Author(s):  
Ausama A Fathallh ◽  
Maha A Mahmood
Keyword(s):  
Dental Caries ◽  
Tobacco Consumption ◽  
Viable Count ◽  
Selective Medium ◽  
Cigarette Smokers ◽  
Cariogenic Bacteria ◽  
Mutans Streptococcus ◽  
The World ◽  
Dmft Index ◽  
Unstimulated Saliva

Background: Waterpipe and cigarette are two products of tobacco consumption, tobacco use has detrimental effects on the oral cavity, numerous studies around the world have reported a significant relationship between smoking and increase dental caries and viable count of cariogenic bacteria, Materials and Methods: unstimulated saliva was collected from 84 subjects and divided equally into three groups waterpipe smokers, cigarette smokers, and non-smokers all of the participants are adult male aged between 25-60 years, dental caries was measured by use DMFT index, while S.mutans and S.sobrinus were isolated by using a selective medium SB 20M (Sugar bacitracin-20 modified) agar Results: this present study showed a significant (p≤0.01). higher DMFT, DT, MT, and FT among cigarette smokers group than both waterpipe smokers and non-smokers groups. The viable count of S mutans was significantly (p≤0.01).higher in the cigarette smokers group followed by the waterpipe smokers group and then the non-smoker's group, while the viable count of S sobrinus showed no statistical differences (P>0.05) between groups. The correlation of DMFT with S.mutans, and S sobrinus count, were significantly positive (p ≤0.05), in the cigarette smokers group only Conclusion: Dental caries increase in cigarette smokers, where the DMFT and S mutans viable count are less affected by waterpipe than cigarette smoking, a positive correlation is found between DMFT and .mutans streptococcus count only in cigarette smokers

scholarly journals Uji Konsentrasi Hambat Minimum Ekstrak Daun Gedi (Abelmoschus manihot L.) terhadap Pertumbuhan Bakteri Streptococcus Mutans

e-GIGI ◽  
2018 ◽  
Vol 6 (1) ◽  
Author(s):  
Helen N. Sekeon ◽  
Heriyannis Homenta ◽  
Michael A. Leman
Keyword(s):  
Dental Caries ◽  
Streptococcus Mutans ◽  
Leaf Extract ◽  
Inhibitory Concentration ◽  
Inhibitory Effect ◽  
Tooth Surface ◽  
Control Group ◽  
Control Group Design ◽  
Cariogenic Bacteria ◽  
Group Design

Abstract: Streptococcus mutans is the most common bacterium that causes dental caries due to its ability to ferment carbohydrates into acid resulting in the decreased pH on the tooth surface. Prevention of dental caries could be achieved by inhibiting the growth of cariogenic bacteria. Various efforts to control and prevent the cariogenic bacteria include the usage of herbal ingredients; one of them is gedi leaves (Abelmoschus manihot L.). These gedi leaves contain bioactive compounds such as flavonoids, alkaloids, steroids, and saponins. This study was aimed to prove that gedi leaf extract had inhibitory effect on the growth of S.mutans and to obtain the minimum inhibitory concentration (MIC) of this extract on the growth of S. mutans. This was a true experimental design with a randomized pretest-posttest control group design. Gedi leaf extract was obtained by maceration method in 96% ethanol. The results showed that gedi leaf extract had an antibacterial effect on the growth of S. mutans. We used turbidimetry, UV-Vis spectrophotometer, and two times of treatment to obtain the MIC of gedi leaf extract on Streptococcus mutans which was 6.25%. Conclusion: Gedi leaf extract could inhibit the growth of S. mutans with a MIC of 6.25%.Keywords: dental caries, gedi leaf extract (Abelmoschus manihot L.), Streptococcus mutans Abstrak: Streptococcus mutans merupakan bakteri yang paling banyak menyebabkan karies gigi karena bakteri ini berkemampuan memfermentasi karbohidrat menjadi asam yang berakibat turunnya pH pada permukaan gigi. Pencegahan karies gigi dapat dicapai dengan menghambat pertumbuhan bakteri kariogenik. Berbagai upaya dilakukan untuk mengen-dalikan dan mencegah bakteri kariogenik, antara lain dengan menggunakan bahan herbal; salah satunya yaitu tanaman gedi (Abelmoschus manihot L.). Daun gedi mengandung senyawa bioaktif antara lain flavonoid, alkaloid, steroid, dan saponin. Penelitian ini bertujuan untuk membuktikan efek inhibisi ekstrak daun gedi terhadap pertumbuhan S. mutans dan mendapatkan konsentrasi hambat minimum (KHM) ekstrak daun gedi terhadap pertumbuhan bakteri S. mutans. Jenis penelitian ini ialah eksperimental murni dengan randomized pretest-posttest control group design. Ekstrak daun gedi dibuat dengan metode maserasi dengan menggunakan etanol 96%. Hasil penelitian menunjukkan bahwa ekstrak daun gedi (Abelmoschus manihot L.) memiliki efek antibakteri dalam menghambat pertumbuhan bakteri Streptococcus mutans. Dengan menggunakan metode turbidimetri dan spektrofotometer UV-Vis dalam 2 (dua) kali perlakuan maka diperoleh KHM ekstrak daun gedi (Abelmoschus manihot L.) terhadap bakteri Streptococcus mutans terdapat pada konsentrasi 6,25%. Simpulan: Ekstrak daun gedi dapat meghambat pertumbuhan Streptococcus mutans dengan KHM pada konsentrasi 6,25%.Kata kunci: karies gigi, ekstrak daun gedi (Abelmoschus manihot L.), Streptococcus mutans

scholarly journals ARE CARIOGENIC BACTERIA THE MAJOR RISK FACTOR TO DENTAL CARIES IN PATIENTS WITH ULCERATIVE COLITIS?

2019 ◽  
Vol 56 (2) ◽  
pp. 118-123
Author(s):  
Eduardo RODRIGUES ◽  
Nuno LARANJEIRA ◽  
Gonçalo NUNES ◽  
Lídia ROQUE-RAMOS ◽  
Ana VIEIRA ◽  
...  
Keyword(s):  
Dental Caries ◽  
Disease Activity ◽  
Eating Habits ◽  
Salivary Flow ◽  
Buffering Capacity ◽  
Mutans Streptococci ◽  
Flow Rates ◽  
Cariogenic Bacteria ◽  
High Prevalence ◽  
Lactobacillus Spp

ABSTRACT BACKGROUND: High prevalence of dental caries in ulcerative colitis (UC) has been attributed to diet and changes in salivary environment. OBJECTIVE: We aimed to characterize the prevalence of dental caries, salivary flow rates, salivary buffering capacity and cariogenic bacteria counts of Mutans streptococci and Lactobacillus spp and to evaluate their relationship with drug therapy, disease activity and duration. METHODS: A cross-sectional study was performed with UC patients followed in a tertiary center. Participants were submitted to a questionnaire (including demographic data, oral hygiene, eating habits) and a clinical observation with assessment of plaque index and Decayed, Missing and Filled Teeth index. Unstimulated/stimulated saliva was collected. Medical records, disease activity (Partial Mayo Score) and disease duration were collected. Laboratory data included salivary flow rates, salivary buffering capacity (CRT® buffer) and cariogenic bacteria count (Mutans streptococci and Lactobacillus spp) in saliva using the CRT® bacteria test (results: high or low counts). RESULTS: Thirty UC patients were recruited. Oral hygiene routines were daily teeth brushing once or more (96.7%) and fluoride toothpaste (73.3%). Decayed, Missing and Filled Teeth index (mean 16.17±6.428) was not affected by the frequency of soft drinks, cakes, sweets and sugars between meals (P>0.2). Long-term disease showed a trend towards higher prevalence of caries (P=0.06). Most presented normal salivary flow rates, unstimulated (73.3%) and stimulated (60.0%), and high salivary buffering capacity (66.7%). Any association was found with age, gender, disease activity, disease duration and drug therapy. High Mutans streptococci and low Lactobacillus spp count were observed in 73.3% and 60% of patients, respectively. Patients with active disease (100%) and longer duration (88.9%) displayed higher Mutans streptococci count. CONCLUSION: The prevalence of dental caries observed in UC patients was significant and did not seem to be influenced by their eating habits. The high prevalence of Mutans streptococci count may be a major risk factor for dental caries and may be looked as part of the UC dysbiosis. Dental care of UC patients should be planned according with this microbiota variation.

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