scholarly journals Effect of Mixing Ratio of Oppositely Charged Block Copolymers on Polyion Complex Micelles for In Vivo Application

Polymers ◽  
2020 ◽  
Vol 13 (1) ◽  
pp. 5
Author(s):  
Noriko Nakamura ◽  
Yuki Mochida ◽  
Kazuko Toh ◽  
Shigeto Fukushima ◽  
Horacio Cabral ◽  
...  
Keyword(s):  
Physicochemical Characteristics ◽  
Charge Ratio ◽  
Structural Variations ◽  
Polyion Complex ◽  
Functional Capabilities ◽  
The Stability ◽  
Biological Performance ◽  
Complex Micelles ◽  
Oppositely Charged

Self-assembled supramolecular structures based on polyion complex (PIC) formation between oppositely charged polymers are attracting much attention for developing drug delivery systems able to endure harsh in vivo environments. As controlling polymer complexation provides an opportunity for engineering the assemblies, an improved understanding of the PIC formation will allow constructing assemblies with enhanced structural and functional capabilities. Here, we focused on the influence of the mixing charge ratio between block aniomers and catiomers on the physicochemical characteristics and in vivo biological performance of the resulting PIC micelles (PIC/m). Our results showed that by changing the mixing charge ratio, the structural state of the core was altered despite the sizes of PIC/m remaining almost the same. These structural variations greatly affected the stability of the PIC/m in the bloodstream after intravenous injection and determined their biodistribution.

Polysaccharides meet dendrimers to fine-tune the stability and release properties of polyion complex micelles

2019 ◽  
Vol 10 (34) ◽  
pp. 4709-4717 ◽  
Author(s):  
Roi Lopez-Blanco ◽  
Marcos Fernandez-Villamarin ◽  
Sorel Jatunov ◽  
Ramon Novoa-Carballal ◽  
Eduardo Fernandez-Megia
Keyword(s):  
Drug Release ◽  
Delivery Systems ◽  
Polyion Complex ◽  
Polyion Complex Micelles ◽  
The Stability ◽  
Complex Micelles ◽  
Kinetics Of ◽  
Fine Tune

Dendritic-polysaccharide PIC micelles represent promising delivery systems where dendritic rigidity and polysaccharide stiffness synchronize to determine the stability of the micelles, their kinetics of intracellular drug release, and cytotoxicity.

scholarly journals A dendrimer–hydrophobic interaction synergy improves the stability of polyion complex micelles

2017 ◽  
Vol 8 (16) ◽  
pp. 2528-2537 ◽  
Author(s):  
Marcos Fernandez-Villamarin ◽  
Ana Sousa-Herves ◽  
Silvia Porto ◽  
Noelia Guldris ◽  
José Martínez-Costas ◽  
...  
Keyword(s):  
Hydrophobic Interaction ◽  
Polyion Complex ◽  
Polyion Complex Micelles ◽  
The Stability ◽  
Complex Micelles

scholarly journals FORMULATION AND OPTIMIZATION OF NIFEDIPINE LOADED NANOCARRIERS

2021 ◽  
pp. 311-317
Author(s):  
ASHWINI JADHAV ◽  
BINOY VARGHESE CHERIYAN
Keyword(s):  
Particle Size ◽  
Zeta Potential ◽  
Physical Stability ◽  
Entrapment Efficiency ◽  
Tween 80 ◽  
Physicochemical Characteristics ◽  
Good Choice ◽  
The Stability

Objective: The main aim of this study to formulate a nifedipine-loaded nanocarrier for improving solubility and bioavailability. Methods: To improve the solubility of drug, nifedipine-loaded nanocarrier (lipotomes) were prepared by using the film lipid hydration technique. lipotomes were prepared by using tween 80, which is used for increasing solubility and cetyl alcohol for lipophilic environment. Drug excipients interaction determined by FTIR. lipotomes were characterized for particle size, Entrapment efficiency and zeta potential. lipotomes were optimized by using Design-Expert 12 software. Optimized formula further lyophilized by using different cyroproyectant to improve the stability and oral administration of the drug. Results: FTIR shows there was no interaction between formulation ingredients. Mean particle size, entrapment efficiency, zeta potential was determined and found to be 308.1 nm, 96.7%, 20.1mV, respectively. Surface morphology of lipotomes was observed by a scanning electron microscope (SEM). Optimized lipotomes was lyophilized with Mannitol (8% w/v) was the ideal cryoprotectant to retain the physicochemical characteristics of the OLT formulation after lyophilization. Conclusion: Nifedipine loaded nanocarrier was successfully prepared, using film hydration method. Which have good particle size, EE% and zeta potential. After lyophilization no significant changes was observed in particle size with good physical stability, so it could be a good choice for conventional drug delivery system by doing further investigation as in vitro and in vivo study

scholarly journals Polyion complex micelles as vectors in gene therapy – pharmacokinetics and in vivo gene transfer

Gene Therapy ◽  
2002 ◽  
Vol 9 (6) ◽  
pp. 407-414 ◽  
Author(s):  
M Harada-Shiba ◽  
K Yamauchi ◽  
A Harada ◽  
I Takamisawa ◽  
K Shimokado ◽  
...  
Keyword(s):  
Gene Therapy ◽  
Gene Transfer ◽  
Polyion Complex ◽  
Polyion Complex Micelles ◽  
Complex Micelles ◽  
In Vivo Gene Transfer

scholarly journals Mannosylated Chitosan Nanoparticles for Delivery of Antisense Oligonucleotides for Macrophage Targeting

2014 ◽  
Vol 2014 ◽  
pp. 1-17 ◽  
Author(s):  
Gyati Shilakari Asthana ◽  
Abhay Asthana ◽  
Dharm Veer Kohli ◽  
Suresh Prasad Vyas
Keyword(s):  
Antisense Oligonucleotides ◽  
Therapeutic Potential ◽  
Transfection Efficiency ◽  
Chitosan Nanoparticles ◽  
Physicochemical Characteristics ◽  
Charge Ratio ◽  
Raw 264.7 Cells ◽  
Macrophage Targeting

The therapeutic potential of antisense oligonucleotides (ASODN) is primarily dependent upon its safe and efficient delivery to specific cells overcoming degradation and maximizing cellular uptakein vivo. The present study focuses on designing mannosylated low molecular weight (LMW) chitosan nanoconstructs for safe ODNs delivery by macrophage targeting. Mannose groups were coupled with LMW chitosan and characterized spectroscopically. Mannosylated chitosan ODN nanoparticles (MCHODN NPs) were formulated by self-assembled method using variousN/Pratio (moles of amine groups of MCH to phosphate moieties of ODNs) and characterized for gel retardation assay, physicochemical characteristics, cytotoxicity and transfection efficiency, and antisense assay. Complete complexation of MCH/ODN was achieved at charge ratio of 1:1 and above. On increasing theN/Pratio of MCH/ODN, particle size of the NPs decreased whereas zeta potential (ZV) increased. MCHODN NPs displayed much higher transfection efficiency into Raw 264.7 cells (bears mannose receptors) than Hela cells and no significant toxicity was observed at all MCH concentrations. Antisense assay revealed that reduction in lipopolysaccharide (LPS) induced serum TNF-αis due to antisense activity of TJU-2755 ODN (sequence complementary to 3′-UTR of TNF-α). These results suggest that MCHODN NPs are acceptable choice to improve transfection efficiencyin vitroandin vivo.

scholarly journals Complexes of Chromium with β-Glycerophosphate: Their Nature and Properties

1970 ◽  
Vol 25 (3) ◽  
pp. 288-292 ◽  
Author(s):  
Leopoldo J. Anghileri
Keyword(s):  
Physicochemical Characteristics ◽  
Chromium Atom ◽  
The Stability

By ion echange analysis, dialysis and electrophoresis of double labelled (32P and 51Cr) chromic β-glycerophosphate complexes, the stability of the bond β-glycerophosphate-chromium under different conditions of olation and oxolation has been studied. The glycerophosphate can form complexes with 1 or 2 moles per chromium atom. Below pH 6.0 the complexes present cationic characteristics, while between pH 6.0 and pH 8.0 they behave as anions. The oxolation provokes a release of β-glycerophosphate from the complex. The physicochemical characteristics of these complexes are reflected in their behaviour “in vivo” when injected into mice.

scholarly journals Insights into Terminal Sterilization Processes of Nanoparticles for Biomedical Applications

Molecules ◽  
2021 ◽  
Vol 26 (7) ◽  
pp. 2068
Author(s):  
Sergio A. Bernal-Chávez ◽  
María Luisa Del Prado-Audelo ◽  
Isaac H. Caballero-Florán ◽  
David M. Giraldo-Gomez ◽  
Gabriela Figueroa-Gonzalez ◽  
...  
Keyword(s):  
Biomedical Applications ◽  
Microbial Contamination ◽  
Average Particle Size ◽  
Physicochemical Characteristics ◽  
Average Particle ◽  
Comprehensive Overview ◽  
Sterile Filtration ◽  
Nonionizing Radiation ◽  
The Stability

Nanoparticles possess a huge potential to be employed in numerous biomedical purposes; their applications may include drug delivery systems, gene therapy, and tissue engineering. However, the in vivo use in biomedical applications requires that nanoparticles exhibit sterility. Thus, diverse sterilization techniques have been developed to remove or destroy microbial contamination. The main sterilization methods include sterile filtration, autoclaving, ionizing radiation, and nonionizing radiation. Nonetheless, the sterilization processes can alter the stability, zeta potential, average particle size, and polydispersity index of diverse types of nanoparticles, depending on their composition. Thus, these methods may produce unwanted effects on the nanoparticles’ characteristics, affecting their safety and efficacy. Moreover, each sterilization method possesses advantages and drawbacks; thus, the suitable method’s choice depends on diverse factors such as the formulation’s characteristics, batch volume, available methods, and desired application. In this article, we describe the current sterilization methods of nanoparticles. Moreover, we discuss the advantages and drawbacks of these methods, pointing out the changes in nanoparticles’ biological and physicochemical characteristics after sterilization. Our main objective was to offer a comprehensive overview of terminal sterilization processes of nanoparticles for biomedical applications.

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