scholarly journals Conversion of Wheat Bran to Xylanases and Dye Adsorbent by Streptomyces thermocarboxydus

Polymers ◽  
2021 ◽  
Vol 13 (2) ◽  
pp. 287
Author(s):  
Thi Ngoc Tran ◽  
Chien Thang Doan ◽  
San-Lang Wang
Keyword(s):  
Wheat Bran ◽  
Radical Scavenging Activity ◽  
Low Cost ◽  
Xylanase Activity ◽  
Radical Scavenging ◽  
Culture Conditions ◽  
Major Product ◽  
Growth Effect ◽  
Xylanase Production ◽  
Initial Ph

Agro-byproducts can be utilized as effective and low-cost nutrient sources for microbial fermentation to produce a variety of usable products. In this study, wheat bran powder (WBP) was found to be the most effective carbon source for xylanase production by Streptomyces thermocarboxydus TKU045. The optimal media for xylanase production was 2% (w/v) WBP, 1.50% (w/v) KNO3, 0.05% (w/v) MgSO4, and 0.10% (w/v) K2HPO4, and the optimal culture conditions were 50 mL (in a 250 mL-volume Erlenmeyer flask), initial pH 9.0, 37 °C, 125 rpm, and 48 h. Accordingly, the highest xylanase activity was 6.393 ± 0.130 U/mL, 6.9-fold higher than that from un-optimized conditions. S. thermocarboxydus TKU045 secreted at least four xylanases with the molecular weights of >180, 36, 29, and 27 kDa when cultured on the WBP-containing medium. The enzyme cocktail produced by S. thermocarboxydus TKU045 was optimally active over a broad range of temperature and pH (40–70 °C and pH 5–8, respectively) and could hydrolyze birchwood xylan to produce xylobiose as the major product. The obtained xylose oligosaccharide (XOS) were investigated for 2,2-diphenyl-1-picrylhydrazyl radical scavenging activity and the growth effect of lactic acid bacteria. Finally, the solid waste from the WBP fermentation using S. thermocarboxydus TKU045 revealed the high adsorption of Congo red, Red 7, and Methyl blue. Thus, S. thermocarboxydus TKU045 could be a potential strain to utilize wheat bran to produce xylanases for XOS preparation and dye adsorbent.

scholarly journals Screening of Culture Conditions for Production of Xylanase from Landfill Soil Bacteria

2019 ◽  
Vol 19 (2) ◽  
pp. 470 ◽  
Author(s):  
Siti Nor Amira Rosli ◽  
Rohaida Che Man ◽  
Nasratun Masngut
Keyword(s):  
Carbon Source ◽  
Moisture Content ◽  
Nitrogen Source ◽  
Incubation Period ◽  
Xylanase Activity ◽  
Nitrogen Sources ◽  
Culture Conditions ◽  
Inoculum Size ◽  
Xylanase Production ◽  
Initial Ph

Culture conditions including initial pH media, incubation period, inoculum size, type of carbon source, type of nitrogen source and its concentration, which affect xylanase production were screened via the one-factor-at-a-time approach. The bacteria used in the production of xylanase was isolated from the landfill site at Sg. Ikan, Kuala Terengganu, Malaysia. Three characterizations of the landfill soil were investigated for their moisture content, ash content, and pH. The culture conditions range used in the experimental work were between 6–30 h for the incubation period, with initial pH between 5–9, inoculum size between 1–20% v/v, carbon, nitrogen sources, and nitrogen source concentration between 1–5% w/v. Xylanase activity was estimated using dinitrosalicylic acid (DNS) based on the release of xylose under standard assay conditions. The landfill soil was observed to have pH between pH 3.4–7.2 with a moisture content between 12.4–33.7% and ash ranged between 3.5–4.3%. Results showed that the highest xylanase activity within studied ranges was recorded at 25.91±0.0641 U/mL with 10% (v/v) inoculum size, 1% (w/v) xylose as sole carbon source, mixture of 1% (w/v) peptone and 0.25% (w/v) ammonium sulphate as nitrogen sources, which was carried out at initial pH of 8.0 for 24 h incubation.

scholarly journals Production of Extracellular Xylanase from Intestinal Bacteria in Termite

1970 ◽  
Vol 25 (2) ◽  
pp. 123-127
Author(s):  
Muhammad Maqsud Hossain ◽  
M Aftab Uddin ◽  
M Abdul Malek ◽  
SAM Khairul Bashar ◽  
Parvin Noor ◽  
...  
Keyword(s):  
Liquid Medium ◽  
Wheat Bran ◽  
Low Cost ◽  
Xylanase Activity ◽  
Intestinal Bacteria ◽  
Maximum Activity ◽  
Carbon Substrate ◽  
Xylanolytic Activity ◽  
Cultural Conditions ◽  
Initial Ph

Although the involvement of intestinal bacteria in the degradation of lignocellulosic materials is poorly understood xylanolytic activity of termite has been studied extensively. The present study was designed to identify potential xylanolytic bacteria from termite intestine and the isolated bacterium was studied with a view to preparing low cost medium and optimizing the cultural conditions in Dubos liquid medium. The bacterium produced highest activity of the enzyme (0.86 U/ml) on birchwood xylan as carbon substrate. Wheat bran a relatively cheap lignocellulosic material was found as good inducer of the enzyme. When grown on 4% wheat bran in Dubos liquid medium with an initial pH 6.0 under shaking incubation at 200 rpm at 40°C for 24 h, the bacterium produced the maximum activity of the extracellular xylanase (1.5 U/ml). These results strongly indicated that the bacterium from the intestine in termite is able synthesize xylanase activity on various substrate and the enzyme production can be increased by optimizing the overall cultivation parameters. Keywords: Xylanase; Termite intestinal bacteriaDOI: http://dx.doi.org/10.3329/bjm.v25i2.4874 Bangladesh J Microbiol, Volume 25, Number 2, December 2008, pp 123-127

scholarly journals Distribution of Phenolic Acids and Antioxidant Activities of Different Bran Fractions from Three Pigmented Wheat Varieties

2018 ◽  
Vol 2018 ◽  
pp. 1-9 ◽  
Author(s):  
Jinli Zhang ◽  
Yan Ding ◽  
Haizhou Dong ◽  
Hanxue Hou ◽  
Xiansheng Zhang
Keyword(s):  
Phenolic Acids ◽  
Wheat Bran ◽  
Phenolic Acid ◽  
Phenolic Content ◽  
Antioxidant Activities ◽  
Radical Scavenging Activity ◽  
Radical Scavenging ◽  
Total Phenolic ◽  
Scavenging Activity ◽  
Wheat Varieties

Phenolic acid profiles and antioxidant activities of outer bran, coarse bran, and shorts from blue, black, and purple wheat were analyzed. Phenolic acids were mainly in the bound form in pigmented wheat bran fractions. Phenolic acid content decreased in the order of outer bran, coarse bran, and shorts for the three pigmented wheat varieties. HPLC analysis of phenolic extracts demonstrated that the bound form of phenolic acids contained more ferulic, isoferulic, and p-coumaric acids compared to their free counterparts. Among the three pigmented wheat varieties, the bran fractions from blue wheat contained higher bound phenolic acids than the other two pigmented wheat bran fractions, except for purple coarse bran. The blue wheat outer bran had the highest total bound phenolic acid of 3458.71 μg/g while the purple wheat shorts had the lowest of 1730.71 μg/g. The contribution of bound phenolic acids to the total phenolic content and antioxidant activity was significantly higher than that of free phenolic acids. Blue wheat bran fractions had the highest radical scavenging activity against DPPH∙ while those of purple wheat gained the highest ABTS∙+ scavenging activity. High correlations were observed between TPC and radical scavenging capacities for DPPH and ABTS (R2>0.85, P<0.05).

scholarly journals Production and Partial Characterization of an Alkaline Xylanase from a Novel FungusCladosporium oxysporum

2016 ◽  
Vol 2016 ◽  
pp. 1-7 ◽  
Author(s):  
Guo-Qiang Guan ◽  
Peng-Xiang Zhao ◽  
Jin Zhao ◽  
Mei-Juan Wang ◽  
Shu-Hao Huo ◽  
...  
Keyword(s):  
Nitrogen Source ◽  
Wheat Bran ◽  
Internal Transcribed Spacer ◽  
Gene Sequence ◽  
Agricultural Waste ◽  
Xylanase Activity ◽  
Maximum Activity ◽  
Alkaline Proteases ◽  
Xylanase Production

A new fungusCladosporium oxysporumGQ-3 producing extracellular xylanase was isolated from decaying agricultural waste and identified based on the morphology and comparison of internal transcribed spacer (ITS) rDNA gene sequence.C. oxysporumproduced maximum xylanase activity of 55.92 U/mL with wheat bran as a substrate and NH4Cl as a nitrogen source. Mg2+improvedC. oxysporumxylanase production.Partially purified xylanase exhibited maximum activity at 50°C and pH 8.0, respectively, and showed the stable activity after 2-h treatment in pH 7.0–8.5 or below 55°C. Mg2+enhanced the xylanase activity by 2% while Cu2+had the highest inhibition ratio of 57.9%. Furthermore,C. oxysporumxylanase was resistant to most of tested neutral and alkaline proteases. Our findings indicated thatCladosporium oxysporumGQ-3 was a novel xylanase producer, which could be used in the textile processes or paper/feed industries.

scholarly journals Optimization of alkali-thermostable and cellulase-free eylanase production from Bacillus Sp.

2012 ◽  
Vol 19 ◽  
pp. 7-14
Author(s):  
SCD Sharma ◽  
MS Shovon ◽  
AKM Asaduzzaman ◽  
MG Sarowar Jahan ◽  
T Yeasmin ◽  
...  
Keyword(s):  
Wheat Bran ◽  
Agar Medium ◽  
Xylanase Activity ◽  
Effect Of Temperature ◽  
Bacillus Sp ◽  
Dilution Technique ◽  
Isolation And Identification ◽  
Xylanase Production ◽  
Optimization Of Process Parameters ◽  
Bacterium Bacillus

Context: To analyze the nutritional and physicochemical parameters for the production of alkali-thermostable and cellulase free xylanase from bacteria. Objectives: The aim of this study was to isolation and identification and of alkali-thermostable and cellulase free xylanase producing bacteria from soil as well as optimization of process parameters for xylanase production. Materials and Methods: The bacterium Bacillus sp. was isolated from soil by serial dilution technique on xylan agar medium and identified by morphological and biochemical studies. The production of xylanase was carried out on xylan broth medium and xylanase activity was assayed by dinitrosalicylic acid (DNS) method. The effect of cultural parameters on the production of xylanase was determined by measuring the activity of xylanase. The effect of temperature and pH on the activity of partially purified xylanase as well as substrate specificity of xylanase were examined. Results: The maximum xylanase production (4000 U/L) by a Bacillus sp. was attained when the medium containing 0.5% wheat bran xylan and peptone at pH 8.0 and 50-55°C within 48-60 h. The partially purified xylanase was optimally active at pH 9.0 and 55°C. The xylanase showed high substrate activity towards wheat bran xylan but no activity towards cellulose, carboxymethyl cellulose and starch. Thus the enzyme was alkali-thermostable and cellulase free xylanase. Conclusion: The results obtained in this study suggest that the Bacillus sp. used is highly potential and useful for the production of cellulase free xylanase. DOI: http://dx.doi.org/10.3329/jbs.v19i0.12994 J. bio-sci. 19: 7-14, 2011

scholarly journals Using Lignosellulose Waste as a Xylanase Production Media of Mold Isolated from Rice Straw of Coastal-field

2018 ◽  
Vol 19 (2) ◽  
pp. 117
Author(s):  
Esti Utarti ◽  
S. Siswanto
Keyword(s):  
Quantitative Analysis ◽  
Rice Straw ◽  
Wheat Bran ◽  
Enzyme Activities ◽  
Activity Index ◽  
Xylanase Activity ◽  
Basic Salt ◽  
Xylanase Production ◽  
Aspergillus Foetidus ◽  
Oat Spelt

Hemicellulose is one of lignocellulose waste component, so that xylanase is one of importance enzyme of lignocellulose waste biodegradation. Molds as main decomposer lignosellulose waste has enzyme activities higher than yeast and bacteria. The aim of the research is to find mold that have xylanolitic activity using lignocellulose waste as media production. The research consist of isolations and screening mols from coastal-field of watu Ulo Jember, xylanase production using lignocellulose waste and idntification of mold which has the highes xylanase activity. A total of 66 molds isolated from rice straw in coastal-field of Watu Ulo Jember. There were screened for their xylanase activity. In semiquantitatively screen on Oat Spelt Xylan plate, the result showed that 62 have xilanolytic activities. Based on clearing zone production, isolates ESW A1 (3.2), ESW A5 (3.1), ESW C 16 (3.26), ESW D4 (3.0) and ESW D15 (3.21) have xilanase activity index higher than others. Furthermore, quantitative analysis using wheat bran, rice straw and baggase in basic salt Mandel’s modification media showed that xylanase activity of isolate ESW D4 was higher on rice straw 3% as substrate production with activity 2.66 U/mL. Isolate ESW D4 identified as Aspergillus foetidus so that called as Aspergillus foetidus ESW D4. Keywords: rice straw, coastal-field, Aspergillus foetidus ESW-D

scholarly journals Effects of Growth Medium Variation on the Nutri-Functional Properties of Microalgae Used for the Enrichment of Ricotta

2021 ◽  
Vol 60 (1) ◽  
Author(s):  
Sheyma Khemiri ◽  
Ines Bouchech ◽  
Nadia Berrejeb ◽  
Mondher Mejri ◽  
Issam Smaali ◽  
...  
Keyword(s):  
Functional Properties ◽  
Biochemical Composition ◽  
Radical Scavenging Activity ◽  
Dairy Product ◽  
Nitrogen Deficiency ◽  
Radical Scavenging ◽  
Biomass Productivity ◽  
Culture Conditions ◽  
Growth Media ◽  
Rich Media

Research background. Microalgae represent an emergent sustainable source of bioactive compounds such as antioxidants, vitamins, minerals, and polyunsaturated fatty acids that can ameliorate the nutritional characteristics of foods. The biochemical composition of microalgae could be modulated by varying the culture conditions to enhance the accumulation of biomolecules of interest. The aim of this work is to optimise the nutri-functional properties of two microalgae with potential utility for food-application. Experimental approach. Nannochloropsis gaditana.L2 and Chlorella sp.SM1 were screened for growth, biochemical composition, and radical scavenging activity employing four different growth media (Algal, BG-11, f/2, and Conway) with different nutrients composition. Additionally, the feasibility of using Chlorella sp.SM1 cultivated in BG-11 medium, in a Mediterranean under-investigated dairy product “ricotta cheese” and its effect on the sensory attributes was investigated. Results and conclusions. Nitrate- and phosphate-rich media (BG-11 and Algal) enhanced the biomass productivity. However, the highest lipid productivity (23.10 mg/(L·day); 11.86 mg/(L·day) for SM1 and L2 respectively) and carbohydrates content (34.79 %; 44.84 % for SM1 and L2 respectively) were obtained with the nitrate-deficient f/2 medium. Regardless of the used medium, the lipidic profile of Chlorella sp.SM1 and Nannochloropsis gaditana.L2 remained adequate for different applications with the presence of C16-18 as main fatty acid (>50 %). Significant increase in oleic acid (C18:1) content was recorded in response to nitrogen deficiency, being the highest in SM1 in f/2 medium (34 %). Nitrogen deficiency was also found to enhance phenolic compounds (48.8 GAE/(mg/g); 35.1 GAE/(mg/g) for SM1 and L2 respectively) and carotenoids contents (2.2 mg/g; 2 mg/g for SM1 and L2 respectively). Due to its interesting antioxidant potential, Chlorella sp.SM1 was used to enrich the ricotta cheese product at different concentrations (0.2 %, 1 % and 1.5 %). The sample with 0.2 % was found to give the most appreciated product. Novelty and scientific contribution. This study permitted the production of an innovative ricotta cheese using Chlorella, as a functional ingredient, without altering the manufacturing diagram while maintaining acceptable sensorial characteristics. The biochemical composition of the used strains varied depending on the culture media’s composition, which permitted the accumulation of phytonutrients of interest.

scholarly journals Optimization, Composition, and Antioxidant Activities of Exo- and Intracellular Polysaccharides in Submerged Culture ofCordyceps gracilis(Grev.) Durieu & Mont.

2015 ◽  
Vol 2015 ◽  
pp. 1-8 ◽  
Author(s):  
Sapan Kumar Sharma ◽  
Nandini Gautam ◽  
Narender Singh Atri
Keyword(s):  
Submerged Culture ◽  
Antioxidant Activities ◽  
Radical Scavenging Activity ◽  
Radical Scavenging ◽  
Reducing Power ◽  
Monosaccharide Composition ◽  
Culture Conditions ◽  
Scavenging Activity ◽  
Orthogonal Experiments ◽  
Nitrogen Ratio

Under present experiments, EPS and IPS production, monosaccharide composition, and antioxidant activities ofC. graciliswere studied for the first time under submerged culture conditions. Effect of different factors on polysaccharides production was studied by orthogonal experiments using one-factor-at-a-time method. Incubation of culture in the medium with capacity 200 mL (675.12 ± 5.01 and 385.20 ± 5.01 mg/L), rotation speed 150 rpm (324.62 ± 3.32 and 254.62 ± 4.62 mg/L), 6-day culture incubation time (445.24 ± 1.11, 216.60 ± 1.71 mg/L), pH 6.0 (374.81 ± 2.52 and 219.45 ± 2.59 mg/L), and temperature 23°C (405.24 ± 1.11 and 215.60 ± 1.71 mg/L) produced higher EPS and IPS, respectively. Maximum EPS and IPS production was observed in the medium supplemented with glucose as a carbon source (464.82 ± 2.12 and 264.42 ± 2.62 mg/L) and yeast extract as a nitrogen source (465.21 ± 3.11 and 245.17 ± 3.24 mg/L), respectively. Carbon to nitrogen ratio for maximum EPS and IPS production was observed as 10 : 1 (395.29 ± 2.15 and 235.62 ± 1.40 mg/L), respectively. Glucose was found to be the major monosaccharide (62.15 ± 7.33%). Both EPS and IPS ofC. gracilisshowed significant DPPH radical scavenging activity, ABTS radical scavenging activity, reducing power, and iron chelating activity.

scholarly journals Spectroscopic characterization of thiol adducts formed in the reaction of 4-methylcatechol with DPPH in the presence of N-acetylcysteine

2018 ◽  
Vol 9 (4) ◽  
pp. 386-393
Author(s):  
Masaki Ichitani ◽  
Hisako Okumura ◽  
Yugo Nakashima ◽  
Hitoshi Kinugasa ◽  
Mitsunori Honda ◽  
...  
Keyword(s):  
Radical Scavenging Activity ◽  
Direct Reduction ◽  
Radical Scavenging ◽  
Spectroscopic Characterization ◽  
Major Product ◽  
Preparative Hplc ◽  
Thiol Compounds ◽  
Activity Enhancement ◽  
Dpph Radical Scavenging

Nucleophiles such as thiol compounds have enhancing effects on the 2,2-diphenyl-1-picrylhydrazyl (DPPH) radical scavenging activities of polyphenols. Several authors have suggested that regeneration of the catechol structure from o-quinone plays a key role in enhanced radical scavenging activity. We therefore explored the reaction of 4-methyl catechol (MC) with DPPH in the presence of N-acetylcysteine (NACys) to clarify the mechanism underlying activity enhancement. Four types of NACys adducts were isolated and purified by preparative HPLC after the reactions reached equilibrium and their structures were characterized spectroscopically using UV-Vis absorption, NMR, and LC-MS. Oxidation of MC using a periodate resin and subsequent reaction with NACys were also studied. LC-MS analyses revealed that a mono-NACys adduct is produced as the major product in the reaction of MC quinone with NACys, and direct reduction by NACys occurs in reactions with NACys MC quinones.

Antimutagenic and radical scavenging activity of wheat bran

2009 ◽  
Vol 37 (1) ◽  
pp. 45-55 ◽  
Author(s):  
L. Brindzová ◽  
M. Zalibera ◽  
T. Jakubík ◽  
M. Mikulášová ◽  
M. Takácsová ◽  
...  
Keyword(s):  
Wheat Bran ◽  
Radical Scavenging Activity ◽  
Radical Scavenging ◽  
Scavenging Activity
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